Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,692 +T 5.5% intergenic (+504/‑427) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6921.→T 5.5% 74.4 ‑0.4 ‑0.0379intergenic (+504/‑427)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/9):  ref base (186/172):  total (198/181)
Fisher's exact test strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 405 total aligned reads displayed.

TCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395592‑1395792
                                                                                                        |                                                                                                    
tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTTCATGTAGTCGGCG.G..GCAGTTCGa                                                                                                       >  2:832486/1‑101 (MQ=255)
 cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.g                                                                                                     >  1:3232103/1‑101 (MQ=255)
  gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.Gc                                                                                                    >  2:1132333/1‑101 (MQ=255)
  gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.Gc                                                                                                    >  2:2596562/1‑101 (MQ=255)
    aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                                  >  1:137468/1‑101 (MQ=255)
       ccccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAg                                                                                               <  2:3839211/99‑1 (MQ=255)
       caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAATTCGA.GCCGCAg                                                                                               <  1:5076069/101‑1 (MQ=255)
         ccTTGCGGATTGTCACTGGCGCGGGGCAAATCGTCGCACCCCACGCGGGGACGAGAATCGAAACATCTGAATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTc                                                                                             <  2:1269876/101‑1 (MQ=255)
          cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCg                                                                                            <  1:6530957/101‑1 (MQ=255)
           ttGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGc                                                                                           >  2:6102644/1‑101 (MQ=255)
             gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                         <  1:4569561/101‑1 (MQ=255)
               ggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTccc                                                                                       >  1:1891353/1‑101 (MQ=255)
                  ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                                    >  1:2060978/1‑101 (MQ=255)
                   tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCACTCGCTCCCCCcg                                                                                   <  1:6268872/101‑1 (MQ=255)
                   tGTCACTTTCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                                   <  1:2435227/101‑1 (MQ=255)
                   tGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                                   <  2:5631423/101‑1 (MQ=255)
                         tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                                             <  2:6528603/101‑1 (MQ=255)
                          ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                            >  1:1844897/1‑101 (MQ=255)
                          ttCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGTATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                            <  2:4977442/101‑1 (MQ=255)
                                 tctgAAGCGGCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:1805845/98‑1 (MQ=255)
                                 tcactcgagccGCTCCCCCCGGGGGGGCGTGGTGCAAAACATCGGCTTGTCCGCTTCGAA..CCTGGTCGC.GTCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  2:2322139/91‑1 (MQ=2)
                                 ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:3071928/101‑1 (MQ=255)
                                 ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  1:5195755/1‑101 (MQ=255)
                                 gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  2:4224277/95‑1 (MQ=2)
                                   cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  2:3502662/1‑101 (MQ=255)
                                    gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  2:2845132/4‑101 (MQ=2)
                                    gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  2:4055764/1‑101 (MQ=255)
                                    gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:461645/1‑101 (MQ=255)
                                    gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:105771/1‑101 (MQ=255)
                                     aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:1652002/1‑101 (MQ=255)
                                     aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:5418038/1‑101 (MQ=255)
                                      tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:5078936/2‑101 (MQ=2)
                                      tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  1:6690887/2‑101 (MQ=2)
                                      tgCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCGCCGCGGGGGCGTGGATCGagc                                                                 >  2:5848258/2‑99 (MQ=2)
                                        cggcGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCTGGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                              <  2:6816205/98‑1 (MQ=25)
                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTG.GCAGGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGAGCGAAAcc                                                               >  2:6877772/1‑100 (MQ=2)
                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                              >  2:2073894/1‑101 (MQ=255)
                                         gTCGCCCCCCCCGCGGGCGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                              <  1:349066/101‑3 (MQ=0)
                                          tCGCGCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                            <  1:2304127/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                           >  1:5692807/1‑101 (MQ=255)
                                              tCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGCCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                        <  1:832510/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                       >  1:98547/1‑101 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                       <  1:144901/101‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                    >  2:1574722/1‑101 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                                   <  1:6005930/101‑1 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                                   >  2:1213817/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                           <  1:159662/101‑1 (MQ=255)
                                                            tgtagATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGCATCTGGCGCAAACg                                          >  1:6837709/5‑101 (MQ=255)
                                                             gTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                         >  2:470439/1‑101 (MQ=255)
                                                             gTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                         >  1:4252927/1‑101 (MQ=255)
                                                             gTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                         >  2:1461481/1‑101 (MQ=255)
                                                              tGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                        >  1:1264640/1‑101 (MQ=255)
                                                              gggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                        <  2:6298452/100‑1 (MQ=255)
                                                              gggATCGAAACATCGGCATGGAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                        <  1:1677431/100‑1 (MQ=255)
                                                               ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       >  2:4514241/1‑101 (MQ=255)
                                                               ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       <  2:2487973/101‑1 (MQ=255)
                                                               ggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                       >  1:4476913/1‑101 (MQ=255)
                                                                gATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACTGGTATGGATCTGGCGCAAACGGaaa                                      >  1:4557292/1‑101 (MQ=255)
                                                                gATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                      >  1:557819/1‑101 (MQ=255)
                                                                 aTCGAAACATCGGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                     <  2:6381990/101‑1 (MQ=255)
                                                                  tCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt                                    >  2:6261544/1‑101 (MQ=255)
                                                                   cGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                   >  2:6994369/1‑101 (MQ=255)
                                                                   cGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                   >  1:5962233/1‑101 (MQ=255)
                                                                   cGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACAGatcgga                                   >  2:3967692/1‑96 (MQ=255)
                                                                     aaaCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                                 >  1:2963383/1‑101 (MQ=255)
                                                                      aaCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                <  1:3987715/101‑1 (MQ=255)
                                                                      aaCATCTGCATGTAGGCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                <  1:1107614/101‑1 (MQ=255)
                                                                      aaCATCTGCATGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                <  1:4309389/101‑1 (MQ=255)
                                                                       aCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                               <  2:2916430/101‑1 (MQ=255)
                                                                       aCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                               <  2:4609199/101‑1 (MQ=255)
                                                                        cttCTGCATGTAGTCGGCG.G..GCAGTCCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                              <  1:2564746/99‑1 (MQ=255)
                                                                        cATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                              >  1:6370234/1‑101 (MQ=255)
                                                                         aTCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                             >  1:6627045/1‑101 (MQ=255)
                                                                           cggCATGTAGCCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                           <  2:3642552/99‑1 (MQ=255)
                                                                           cTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGGCTGtcgtcg                           >  2:4103895/1‑101 (MQ=255)
                                                                           cTGCAGGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                           <  2:3415184/101‑1 (MQ=255)
                                                                            tGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                          >  2:5899082/1‑101 (MQ=255)
                                                                            tGCATGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                          <  2:4830589/101‑1 (MQ=255)
                                                                             gCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtagatcgg                         >  1:1195874/1‑94 (MQ=255)
                                                                             gCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                         >  2:1930570/1‑101 (MQ=255)
                                                                               aTGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                       >  1:6720632/1‑101 (MQ=255)
                                                                               aTGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                       <  1:7010128/101‑1 (MQ=255)
                                                                                tattgtgggcg.g..ACAGTCCGA.GCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccc                      <  2:3199969/94‑1 (MQ=37)
                                                                                 gTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                     >  1:2189865/1‑101 (MQ=255)
                                                                                   aggcggcg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   <  2:2801481/98‑1 (MQ=255)
                                                                                   agccggcg.g..GCGGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   <  1:4809300/98‑1 (MQ=255)
                                                                                   aGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   >  2:6751660/1‑101 (MQ=255)
                                                                                   aGTCGGCG.G..GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   <  2:1863771/101‑1 (MQ=255)
                                                                                    tgggcggcg..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                   <  2:6665166/93‑1 (MQ=255)
                                                                                         cg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg             >  2:3157127/1‑101 (MQ=255)
                                                                                         cg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg             <  1:4921565/101‑1 (MQ=255)
                                                                                               gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCg          <  1:4054116/101‑1 (MQ=255)
                                                                                                cAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt         <  2:5195490/101‑1 (MQ=255)
                                                                                                cAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt         >  1:3737528/1‑101 (MQ=255)
                                                                                                 aGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGGGTg        >  1:3297035/1‑101 (MQ=255)
                                                                                                 aGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg        >  1:5317281/1‑101 (MQ=255)
                                                                                                    gggA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt     <  1:6155760/99‑1 (MQ=255)
                                                                                                     ggA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc    <  1:3895112/100‑1 (MQ=255)
                                                                                                     cGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc    >  1:2678117/1‑101 (MQ=255)
                                                                                                      gA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  2:137465/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
TCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395592‑1395792

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG