Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,867 +T 1.4% intergenic (+679/‑252) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8671.→T 1.4% 14.0 0.0 ‑0.0443intergenic (+679/‑252)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (211/226):  total (214/229)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.59e-01

Only 100 of 480 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395767‑1395967
                                                                                                     |                                                                                                             
 cTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg.c                                                                                                              >  2:1270132/1‑101 (MQ=255)
     ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGGGTGGATCGAAACTGCg.ccgc.c                                                                                                         >  1:5173274/1‑101 (MQ=255)
       ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                       <  1:590738/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                      <  1:2421494/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                      <  1:4760016/101‑1 (MQ=255)
         gcgGGGGCGTGGATTGAAAGCCGCGGGGACGGTGGGCAGTGAAAGCGGCGGTGGACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.CCGC.CTATc                                                                                                     <  2:1917150/101‑1 (MQ=16)
            ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                                  >  1:1821690/1‑101 (MQ=255)
             gggCGTTGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAGCTGCG.CCGC.CTATCGCAt                                                                                                 <  1:6723444/101‑1 (MQ=255)
             gggCGTGGATCGAAACGTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                 <  2:4559426/101‑1 (MQ=255)
             gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                 <  2:714684/101‑1 (MQ=255)
             gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.ACGC.CTATCGCAt                                                                                                 <  1:4542491/101‑1 (MQ=255)
              ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                                <  2:534464/101‑1 (MQ=255)
               gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                               <  1:4055940/101‑1 (MQ=255)
                cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                              >  1:4431916/1‑101 (MQ=255)
                cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                              <  2:5069139/101‑1 (MQ=255)
                 gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                             >  1:1375471/1‑101 (MQ=255)
                     aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                      <  1:6006397/101‑1 (MQ=255)
                        gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                   >  2:4209174/1‑101 (MQ=255)
                         aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                                  >  2:6606208/1‑101 (MQ=255)
                         aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgagc                                                                                  >  2:939758/1‑101 (MQ=255)
                            ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgc.c.gc                                                                             <  2:5394707/101‑1 (MQ=255)
                                      gAGTTGCCAGTTATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTc                                                                <  2:5688779/101‑1 (MQ=255)
                                           gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTcccccc                                                           >  1:3455718/1‑101 (MQ=255)
                                           gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTcccccc                                                           <  2:2963274/101‑1 (MQ=255)
                                            ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                          <  2:557807/101‑1 (MQ=255)
                                              aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcggg                                                        >  1:3886385/1‑99 (MQ=255)
                                                  aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCggggg                                                    >  2:5188918/1‑101 (MQ=255)
                                                   tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCTGGGGc                                                   <  1:5637625/101‑1 (MQ=255)
                                                   tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGc                                                   >  2:2481263/1‑101 (MQ=255)
                                                   tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGc                                                   >  2:413786/1‑101 (MQ=255)
                                                     gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGt                                                 >  2:1004433/1‑101 (MQ=255)
                                                      aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTg                                                <  2:6837355/101‑1 (MQ=255)
                                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:6316965/91‑1 (MQ=9)
                                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:3250742/91‑1 (MQ=9)
                                                       gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:2665653/11‑101 (MQ=9)
                                                        gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  2:4661403/1‑101 (MQ=255)
                                                        gCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAGACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:5085521/101‑1 (MQ=255)
                                                        gCCGTGGACGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  2:1707508/1‑101 (MQ=255)
                                                        ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:966489/92‑1 (MQ=9)
                                                         caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCGCC.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1768585/93‑1 (MQ=2)
                                                         caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:2379022/93‑1 (MQ=9)
                                                          gcccggcGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:1118135/95‑1 (MQ=2)
                                                          cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGC.CATCGAG.C.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  2:4040267/7‑101 (MQ=0)
                                                          aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:1563305/8‑101 (MQ=9)
                                                           gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  2:4813314/1‑101 (MQ=255)
                                                           gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:1492773/1‑101 (MQ=255)
                                                           gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.CCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:3635253/1‑101 (MQ=255)
                                                            cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  2:3621060/96‑1 (MQ=9)
                                                            ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  2:5117400/5‑101 (MQ=2)
                                                            ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:6999633/97‑1 (MQ=2)
                                                             ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:1930642/101‑1 (MQ=255)
                                                             ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  2:1366227/5‑101 (MQ=9)
                                                             cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:4892822/98‑1 (MQ=2)
                                                              tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:3828038/4‑101 (MQ=9)
                                                              ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:1100936/3‑101 (MQ=2)
                                                              gACGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:145695/101‑1 (MQ=255)
                                                              gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:343072/1‑101 (MQ=255)
                                                               gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:1155281/100‑1 (MQ=2)
                                                               atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:3108723/99‑1 (MQ=9)
                                                               atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  1:3583044/3‑101 (MQ=9)
                                                                tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      <  2:3554508/100‑2 (MQ=9)
                                                                tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      >  2:5975960/2‑100 (MQ=9)
                                                                tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      >  1:4688879/2‑100 (MQ=9)
                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                      >  2:3250990/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                     >  1:285072/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                     >  1:5553267/1‑101 (MQ=255)
                                                                  tCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                    <  1:6178001/101‑1 (MQ=255)
                                                                  ccGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                    <  1:2110095/100‑1 (MQ=255)
                                                                   cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                   >  1:3744026/1‑101 (MQ=9)
                                                                   cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                   <  2:3951784/101‑1 (MQ=9)
                                                                   cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                   <  2:1659801/101‑1 (MQ=255)
                                                                     ctcgcgccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.CTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                 <  2:5855268/95‑1 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                 <  2:2026815/101‑1 (MQ=255)
                                                                      tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                <  2:1050617/98‑2 (MQ=9)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                >  2:2631060/1‑101 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACTGCG.CCCC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                              <  1:3309835/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgc                             <  1:2019990/101‑5 (MQ=9)
                                                                         ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                             <  2:6411699/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                             <  1:4209369/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                           <  1:3811152/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                              <  2:6021689/101‑10 (MQ=2)
                                                                             gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                         <  1:7043461/101‑9 (MQ=2)
                                                                               gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                       <  2:6124289/101‑11 (MQ=2)
                                                                               gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                       >  1:5411603/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     <  1:2534940/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     <  1:6521361/101‑1 (MQ=255)
                                                                                 gggCGCTGATCGAACCTGCC.CCGC.CTGCCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                     <  2:6725519/101‑3 (MQ=25)
                                                                                  ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                    >  2:4402061/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                    <  1:1652063/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                   <  1:1002962/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATGGCCTACGTc                  >  1:6844405/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAACGGCG.CCGC.CTAGCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                  <  2:2234096/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                 >  1:2986004/1‑101 (MQ=255)
                                                                                      tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                >  1:5784231/1‑101 (MQ=255)
                                                                                       ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg               >  1:1436211/1‑101 (MQ=255)
                                                                                       ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg               >  1:6454286/1‑101 (MQ=255)
                                                                                             aaaCTGCT.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg         <  2:5621019/101‑1 (MQ=255)
                                                                                               aCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGAc       >  2:3361285/1‑101 (MQ=255)
                                                                                                cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt      >  2:788534/1‑101 (MQ=255)
                                                                                                    g.ccgc.cTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc  <  1:401760/101‑1 (MQ=255)
                                                                                                     |                                                                                                             
GCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395767‑1395967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG