Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,871 +G 1.3% intergenic (+683/‑248) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8711.→G 1.3% 8.6 0.0 ‑0.1451intergenic (+683/‑248)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (214/231):  total (217/234)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.77e-01

Only 100 of 475 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395772‑1395971
                                                                                                                                                                                                             
ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGGGTGGATCGAAACTGCgccgcc                                                                                                          >  1:5173274/1‑101 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                                                                         <  2:1103467/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                        <  1:1922053/101‑1 (MQ=255)
   cgcgGGGGCGGGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                       <  2:6369910/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                                                                      >  2:5361509/1‑101 (MQ=255)
       ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                   >  1:1821690/1‑101 (MQ=255)
       ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                   >  1:4007822/1‑101 (MQ=255)
        gggCGTTGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAGCTGCGCCGCCTATCGCAt                                                                                                  <  1:6723444/101‑1 (MQ=255)
        gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                  <  2:714684/101‑1 (MQ=255)
          gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                <  1:4055940/101‑1 (MQ=255)
          gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                <  1:4884305/101‑1 (MQ=255)
           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                               >  2:5857493/1‑101 (MQ=255)
           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                               >  1:4431916/1‑101 (MQ=255)
            gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                              >  1:1375471/1‑101 (MQ=255)
                 tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                                      >  1:2124028/1‑101 (MQ=255)
                  cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                     <  2:5093212/101‑1 (MQ=255)
                  cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                     <  2:2018305/101‑1 (MQ=255)
                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                    <  2:1381072/101‑1 (MQ=255)
                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                    >  2:4209174/1‑101 (MQ=255)
                    aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                   >  2:6606208/1‑101 (MQ=255)
                    aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgagc                                                                                   >  2:939758/1‑101 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.Gt                                                                         >  2:5723030/1‑101 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.Gt                                                                         <  2:4048292/101‑1 (MQ=255)
                               gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGc                                                                      >  1:1968097/1‑101 (MQ=255)
                                  aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcc                                                                   <  2:1272347/101‑1 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                               <  2:2963274/101‑1 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                               >  2:5992330/1‑101 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                               >  1:3455718/1‑101 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                               <  1:2435171/101‑1 (MQ=255)
                                        cAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCcgc                                                             >  1:5070080/1‑101 (MQ=255)
                                         aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCcggg                                                            >  1:3886385/1‑99 (MQ=255)
                                              tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCTGGGGc                                                       <  1:5637625/101‑1 (MQ=255)
                                              tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGc                                                       >  2:413786/1‑101 (MQ=255)
                                              tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGc                                                       >  2:2481263/1‑101 (MQ=255)
                                                gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                     >  2:1004433/1‑101 (MQ=255)
                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:4520520/101‑1 (MQ=255)
                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  2:6837355/101‑1 (MQ=255)
                                                  gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  1:3250742/91‑1 (MQ=9)
                                                  gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                   >  2:2665653/11‑101 (MQ=9)
                                                   cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                     <  2:5580573/93‑1 (MQ=2)
                                                   ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  1:966489/92‑1 (MQ=9)
                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                    >  2:5462037/8‑101 (MQ=2)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:1768585/93‑1 (MQ=2)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 >  1:3109116/9‑101 (MQ=9)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  2:2379022/93‑1 (MQ=9)
                                                     gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:4605778/95‑1 (MQ=2)
                                                     aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  2:1563305/8‑101 (MQ=9)
                                                     aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:5118312/94‑1 (MQ=9)
                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCCCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:3635253/1‑101 (MQ=255)
                                                       tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:5753739/1‑101 (MQ=255)
                                                         tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            >  2:3828038/4‑101 (MQ=9)
                                                         tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                            >  2:3183540/4‑101 (MQ=9)
                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:5879403/3‑101 (MQ=2)
                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:1100936/3‑101 (MQ=2)
                                                         gACGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:145695/101‑1 (MQ=255)
                                                         gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            >  2:343072/1‑101 (MQ=255)
                                                          gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  1:1155281/100‑1 (MQ=2)
                                                          atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:3583044/3‑101 (MQ=9)
                                                          aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:6453250/101‑1 (MQ=255)
                                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          <  2:3554508/100‑2 (MQ=9)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                         >  1:6804491/1‑101 (MQ=255)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                         >  1:285072/1‑101 (MQ=255)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGac                                        >  2:472775/1‑100 (MQ=255)
                                                              cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                       >  1:3744026/1‑101 (MQ=9)
                                                              cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                       <  2:1659801/101‑1 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                    >  2:2631060/1‑101 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACTGCGCCCCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                  <  1:3309835/101‑1 (MQ=255)
                                                                   accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                  >  2:1042178/2‑98 (MQ=9)
                                                                    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                 <  1:4209369/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                <  2:3609009/101‑6 (MQ=9)
                                                                     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                <  1:1480303/101‑1 (MQ=255)
                                                                      ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                               <  1:3811152/101‑1 (MQ=255)
                                                                      ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                  <  2:6021689/101‑10 (MQ=2)
                                                                       cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                              <  2:700176/101‑8 (MQ=2)
                                                                        gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                             <  1:7043461/101‑9 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                           <  2:6124289/101‑11 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                           <  2:1899004/101‑11 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                           >  1:5411603/1‑101 (MQ=255)
                                                                          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                           <  1:6053588/101‑1 (MQ=255)
                                                                           ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                          >  1:5566782/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                         <  1:2534940/101‑1 (MQ=255)
                                                                            gggCGCTGATCGAACCTGCCCCGCCTGCCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                         <  2:6725519/101‑3 (MQ=25)
                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                        >  2:4402061/1‑101 (MQ=255)
                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                        <  1:1652063/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                       <  1:1002962/101‑1 (MQ=255)
                                                                               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                      >  2:4003795/1‑101 (MQ=255)
                                                                                gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                     >  1:2986004/1‑101 (MQ=255)
                                                                                 tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                    >  1:650511/1‑101 (MQ=255)
                                                                                  ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                   >  1:1436211/1‑101 (MQ=255)
                                                                                       gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAggg              >  1:4735180/1‑99 (MQ=255)
                                                                                       gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg              <  1:5998090/101‑1 (MQ=255)
                                                                                        aaaCTGCTCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg             <  2:5621019/101‑1 (MQ=255)
                                                                                        aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg             <  2:2189770/101‑1 (MQ=255)
                                                                                          aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGAc           >  2:3361285/1‑101 (MQ=255)
                                                                                           cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt          >  2:788534/1‑101 (MQ=255)
                                                                                            tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg         >  2:6440369/1‑101 (MQ=255)
                                                                                            tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg         >  2:5217067/1‑101 (MQ=255)
                                                                                                 cgccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc    <  1:4661642/101‑1 (MQ=255)
                                                                                                  gccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCCACGTCGGGCAGTGGACTggccact   >  2:1726214/1‑96 (MQ=255)
                                                                                                   ccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc  <  2:4818445/101‑1 (MQ=255)
                                                                                                                                                                                                             
CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395772‑1395971

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG