Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,939 +A 10.6% coding (821/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9391.→A10.6% 221.0 ‑1.6 ‑0.8519coding (821/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/34):  ref base (221/243):  total (242/277)
Fisher's exact test strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.67e-01

Only 100 of 561 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCT  >  NC_014034/1396839‑1397039
                                                                                                      |                                                                                                              
 cgccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                               <  1:757969/99‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                               <  1:4871680/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                               >  2:573262/1‑101 (MQ=255)
  tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                              <  1:1677331/101‑1 (MQ=255)
  cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                              <  2:898066/100‑1 (MQ=255)
      cccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCg.g                                                                                                         <  1:5811132/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc.c                                                                                                      <  1:4807718/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc.c                                                                                                      <  2:4234873/101‑1 (MQ=255)
           gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                   >  1:4086434/1‑101 (MQ=255)
             gggCGTTGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..Tc                                                                                               <  2:5095732/101‑1 (MQ=255)
                 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCt                                                                                           >  2:4186251/1‑101 (MQ=255)
                 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCt                                                                                           >  2:6047217/1‑101 (MQ=255)
                  tGGATCTAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCt.t                                                                                         <  2:5943423/101‑1 (MQ=255)
                   ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCC.GCGCG.GC.CGCA..TCGGCtcc                                                                                         >  2:2295174/1‑99 (MQ=255)
                   ggATCGAAACCGGTCCAGAGATAGACCGCGCGCACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.Tc                                                                                        <  1:3402864/101‑1 (MQ=255)
                    gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.Tc.c                                                                                      <  2:6844051/101‑1 (MQ=255)
                    gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.Tc.c                                                                                      <  1:1314197/101‑1 (MQ=255)
                        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.Ct                                                                                 <  2:1777542/101‑1 (MQ=255)
                             cGGTACAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTg                                                                          >  2:1644415/1‑101 (MQ=255)
                               gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.Gt                                                                       <  1:1014352/101‑1 (MQ=255)
                                tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                      <  2:2187321/101‑1 (MQ=255)
                                   agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCt                                                                   >  1:148271/1‑101 (MQ=255)
                                      gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccc                                                                >  1:1145102/1‑101 (MQ=255)
                                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccc                                                               <  2:1337654/101‑1 (MQ=255)
                                        tagacccgccgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                              <  2:3794856/92‑1 (MQ=255)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                             <  2:2706940/101‑1 (MQ=255)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                             <  1:891177/101‑1 (MQ=255)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                             >  1:3363613/1‑101 (MQ=255)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                             >  2:702831/1‑101 (MQ=255)
                                          gACCGCGCGGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                            <  2:5670868/101‑1 (MQ=255)
                                                gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                      <  2:2060205/101‑1 (MQ=255)
                                                 cgGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCCGGGGc                                                     <  2:3514505/101‑1 (MQ=255)
                                                   gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CCC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCgg                                                   >  2:2152926/1‑100 (MQ=255)
                                                      tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                 >  2:1404911/10‑101 (MQ=0)
                                                      attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:3878794/91‑1 (MQ=0)
                                                        tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  2:6433906/93‑1 (MQ=2)
                                                        tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  2:1244526/93‑1 (MQ=2)
                                                        cctcGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:2742438/101‑1 (MQ=255)
                                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  2:3216650/94‑1 (MQ=2)
                                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                             >  1:1936314/8‑101 (MQ=2)
                                                         cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  2:4607307/7‑101 (MQ=2)
                                                         cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGG.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  2:2634859/7‑101 (MQ=0)
                                                          tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGTGGCGTGGATCGa                                            <  2:103894/101‑1 (MQ=255)
                                                          ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:1230615/96‑1 (MQ=2)
                                                          ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:6287605/96‑1 (MQ=2)
                                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:3669236/101‑1 (MQ=255)
                                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  1:2200210/1‑101 (MQ=255)
                                                           acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            <  1:2400198/96‑1 (MQ=9)
                                                            ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:161251/98‑1 (MQ=2)
                                                            ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:5204680/98‑1 (MQ=2)
                                                            ggtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG..AGTGCG.TCTGGA.ACA..CGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:5024580/97‑1 (MQ=2)
                                                            cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          <  1:223214/97‑1 (MQ=2)
                                                             gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         >  1:6151766/1‑101 (MQ=255)
                                                              gcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         <  1:6676257/100‑1 (MQ=2)
                                                              ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         >  2:3368841/3‑101 (MQ=9)
                                                               cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                       <  1:3125862/98‑1 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                      <  2:4965321/101‑4 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                      <  2:5790450/101‑4 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                       <  2:6528541/101‑3 (MQ=9)
                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                       <  1:2852264/101‑3 (MQ=2)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                      <  1:440013/101‑4 (MQ=9)
                                                                 ccGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                        <  1:5689420/100‑1 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                       >  1:6984598/1‑100 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                    <  2:1130326/101‑1 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                    <  1:6895816/101‑6 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                   <  1:2640560/101‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGcc                                  >  2:1852078/1‑100 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                  <  2:4050620/101‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatcgga                                  >  1:3202270/1‑93 (MQ=2)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                 >  1:6443168/1‑101 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                  >  1:5472416/1‑94 (MQ=9)
                                                                      ccccccGGGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                 >  1:5982194/1‑93 (MQ=9)
                                                                      ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                   <  2:4688640/101‑3 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CC.AGGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                   <  2:3173614/101‑3 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCGCCCGCGGGGGCGTGGATCGAAACATCAGcccc                                >  1:4741080/1‑98 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                >  1:4220069/1‑101 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                <  2:248111/101‑10 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                 <  2:1984855/101‑5 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaagat                                 >  1:7083844/1‑97 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                               >  2:2440326/1‑91 (MQ=2)
                                                                         cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                <  2:287294/101‑6 (MQ=0)
                                                                         cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                <  1:2098123/101‑6 (MQ=0)
                                                                          ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                               <  2:553732/101‑7 (MQ=0)
                                                                           cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                           <  1:4538050/101‑1 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                         <  1:5591954/101‑1 (MQ=255)
                                                                              gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                        >  1:6383036/1‑101 (MQ=255)
                                                                               ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                       >  1:3801482/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                      <  1:5312042/101‑1 (MQ=255)
                                                                                gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                      <  2:6670335/101‑1 (MQ=255)
                                                                                    gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                  >  1:3933410/1‑101 (MQ=255)
                                                                                      ggATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                >  2:2983762/1‑101 (MQ=255)
                                                                                      ggATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                >  1:3051146/1‑101 (MQ=255)
                                                                                        aTCGAACCA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg              <  1:1852150/101‑1 (MQ=255)
                                                                                           gAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg           >  2:1512950/1‑101 (MQ=255)
                                                                                           gAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg           <  2:61725/101‑1 (MQ=255)
                                                                                            aaaCA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt          >  2:749848/1‑101 (MQ=255)
                                                                                             aaCA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc         <  1:2476756/101‑1 (MQ=255)
                                                                                               cA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg       <  2:5332438/101‑1 (MQ=255)
                                                                                               cA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg       <  2:3056437/101‑1 (MQ=255)
                                                                                                    cT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc   >  2:3561792/1‑101 (MQ=255)
                                                                                                      |                                                                                                              
GCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCT  >  NC_014034/1396839‑1397039

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG