BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,964	+A	11.5%	coding (846/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,964	2	.→A	11.5%	243.1 ‑2.3 ‑0.6	524	coding (846/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/26): ref base (209/255): total (243/281)
Fisher's exact test strand distribution p-value = 9.95e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.49e-01

Only 100 of 557 total aligned reads displayed.


AAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1396864‑1397064


aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                                                         <  1:2678895/101‑1 (MQ=255)


   ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..Cgg                                                                                                    >  2:122390/1‑100 (MQ=255)


     ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg.g                                                                                                 >  1:1651645/1‑101 (MQ=255)


      gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.Gt                                                                                                <  1:1014352/101‑1 (MQ=255)


       tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTc                                                                                               >  1:2188660/1‑101 (MQ=255)


        ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCg                                                                                              >  2:4008051/1‑101 (MQ=255)


          cgagaTAGCCCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCt                                                                                            <  2:3160027/100‑1 (MQ=255)


          agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCt                                                                                            >  1:148271/1‑101 (MQ=255)


                aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                      >  1:3136302/1‑101 (MQ=255)


                aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                      >  2:2476662/1‑101 (MQ=255)


                  aCCGCGCGGCCCGCCGCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcgc                                                                                    <  2:6443827/101‑1 (MQ=255)


                            ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:3973994/91‑1 (MQ=0)


                            cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GGCGCTCCCCCCGCGGGGGCGTgg                                                                          >  1:5705615/1‑101 (MQ=255)


                             tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  1:6623937/10‑101 (MQ=0)


                             attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:4989459/11‑101 (MQ=0)


                             attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:335455/11‑101 (MQ=0)


                              gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:5626826/10‑101 (MQ=9)


                               tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  2:3516712/9‑101 (MQ=2)


                               tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:1279405/9‑101 (MQ=9)


                               gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  1:4768588/94‑1 (MQ=2)


                               gcggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:2210960/94‑1 (MQ=2)


                               cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:6812646/101‑1 (MQ=255)


                                ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:3216650/94‑1 (MQ=2)


                                ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:4822804/101‑1 (MQ=255)


                                ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:7086899/101‑1 (MQ=255)


                                caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:6233245/8‑101 (MQ=9)


                                 ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:3392758/96‑1 (MQ=2)


                                 ccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:2951849/100‑1 (MQ=255)


                                 aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:1954229/7‑101 (MQ=9)


                                   aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  2:2154608/101‑1 (MQ=255)


                                    tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:3884219/3‑101 (MQ=2)


                                    gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  >  1:6151766/1‑101 (MQ=255)


                                    actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:5521182/4‑101 (MQ=9)


                                     cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                 <  2:4640997/99‑2 (MQ=2)


                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                >  2:2687303/1‑100 (MQ=255)


                                      cGTCGCTCCCCCCGCCGGGCGGTGGATCCAAACA.GCC..GCTCG.AC.CGCA.TCGCACCG.GGTCC.CCG..CGCC.GCCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                                 <  2:2319531/101‑2 (MQ=2)


                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  >  2:3620972/1‑101 (MQ=2)


                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                               <  2:6108605/101‑4 (MQ=2)


                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                               >  1:6979913/1‑98 (MQ=2)


                                       gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  1:1169098/101‑1 (MQ=2)


                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                              <  1:23042/101‑1 (MQ=255)


                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                              <  1:222824/101‑1 (MQ=255)


                                        ccGCTCCCCCCGGCGGGGCGTGGATCGAAACA.CCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                              <  2:2533431/100‑1 (MQ=255)


                                        ccGCTCCCCCCCGGGGGGCGTGGACCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                              <  2:1098792/100‑1 (MQ=255)


                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                >  2:1992733/1‑100 (MQ=2)


                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                              >  1:854282/1‑97 (MQ=2)


                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                             <  1:6895816/101‑6 (MQ=2)


                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                               >  2:2069492/1‑99 (MQ=2)


                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                             >  2:1907558/1‑96 (MQ=2)


                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                            >  1:2853839/1‑101 (MQ=255)


                                          gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                             >  2:6216243/1‑96 (MQ=9)


                                            tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatcgga                                                           >  1:3202270/1‑93 (MQ=2)


                                            tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccac                                                           >  1:2496554/1‑94 (MQ=2)


                                             ccccccGGGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                          >  1:5982194/1‑93 (MQ=9)


                                             ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                         >  2:893597/1‑92 (MQ=2)


                                             ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                         >  2:744687/1‑92 (MQ=2)


                                             ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                          <  2:140157/101‑9 (MQ=9)


                                             ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                          >  1:5840841/1‑93 (MQ=9)


                                              cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                        <  1:960121/101‑1 (MQ=255)


                                               ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                       <  2:148268/101‑1 (MQ=255)


                                               ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                        <  2:5507177/101‑11 (MQ=2)


                                               ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                        >  1:6429325/1‑91 (MQ=2)


                                                cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                         <  1:2098123/101‑6 (MQ=0)


                                                cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                         <  2:287294/101‑6 (MQ=0)


                                                 ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                        <  1:244059/101‑7 (MQ=0)


                                                 ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                        <  2:7025323/101‑7 (MQ=0)


                                                  cgggggggCGTGGATCGAAACC.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAACAACAGCTCGTCGtt                                                    <  1:5072431/98‑1 (MQ=38)


                                                  cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                                    <  1:4362662/101‑1 (MQ=255)


                                                     ggggcCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                                 <  2:5015596/101‑1 (MQ=255)


                                                       gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                               <  2:6670335/101‑1 (MQ=255)


                                                       gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                               <  1:340206/101‑1 (MQ=255)


                                                         gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                             >  1:5332705/1‑101 (MQ=255)


                                                           gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTCGTCGTTGGTCAGCCg                                           >  2:5755535/1‑101 (MQ=255)


                                                           gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                           >  2:4231118/1‑101 (MQ=255)


                                                           gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                           >  1:4452202/1‑101 (MQ=255)


                                                            tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                          >  2:1671299/1‑101 (MQ=255)


                                                             ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                         >  1:3051146/1‑101 (MQ=255)


                                                              gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                        <  2:595570/101‑1 (MQ=255)


                                                               aTCGAACCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                       <  1:1852150/101‑1 (MQ=255)


                                                                 cGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                     >  1:3796274/1‑101 (MQ=255)


                                                                 cGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                     >  2:4089102/1‑101 (MQ=255)


                                                                   aaaCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                                   <  2:1333742/101‑1 (MQ=255)


                                                                      cA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                                <  2:3056437/101‑1 (MQ=255)


                                                                       a.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTAGTCAGCCGGACGATGTCCGt                               >  1:5631395/1‑101 (MQ=255)


                                                                                  g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                      <  2:5839984/101‑1 (MQ=255)


                                                                                  g.gC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                      <  2:5300338/101‑1 (MQ=255)


                                                                                       cGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc                   <  1:4186446/101‑1 (MQ=255)


                                                                                          a.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg                >  2:4707696/1‑101 (MQ=255)


                                                                                            tCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg               >  1:2782708/1‑101 (MQ=255)


                                                                                             cG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg              >  1:462086/1‑101 (MQ=255)


                                                                                             cG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg              >  2:154021/1‑101 (MQ=255)


                                                                                                   t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg          <  2:4946475/101‑1 (MQ=255)


                                                                                                     tctgC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCTCTCCCCCCGCGGGGGCGTgg         <  1:6380426/98‑1 (MQ=255)


                                                                                                        gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc      <  1:3621509/101‑1 (MQ=255)


                                                                                                        gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:3933235/101‑1 (MQ=255)


                                                                                                        gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:6525617/101‑1 (MQ=255)


                                                                                                         c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  2:4425667/101‑1 (MQ=255)


                                                                                                         c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  2:3820389/101‑1 (MQ=255)


                                                                                                         c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  1:1600350/101‑1 (MQ=255)


                                                                                                            tG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  1:5259699/101‑1 (MQ=255)


AAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1396864‑1397064

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG