Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,483 +T 12.0% coding (1365/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4831.→T12.0% 209.2 0.0 ‑0.6424coding (1365/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/22):  ref base (213/160):  total (242/182)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.53e-01

Only 100 of 454 total aligned reads displayed.

GGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGG  >  NC_014034/1397383‑1397583
                                                                                                       |                                                                                                        
 gggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.c                                                                                                         >  1:5267588/1‑101 (MQ=255)
 gggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.c                                                                                                         <  1:3800416/101‑1 (MQ=255)
  ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                                        <  1:4928321/101‑1 (MQ=255)
  ggCGTGGATCGAAACTCTTCAATGGAATTGGGAAAACCGTCCGCGACATGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.TCCACGCAA.cc                                                                                                        <  2:697292/101‑1 (MQ=37)
   gCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCg                                                                                                       <  2:5404642/101‑1 (MQ=255)
    cGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGc                                                                                                      >  1:4742898/1‑101 (MQ=255)
       ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                                   >  2:521290/1‑101 (MQ=255)
        gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTg                                                                                                  >  2:86262/1‑101 (MQ=255)
         aTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGc                                                                                                 <  2:6636854/101‑1 (MQ=255)
         aTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGc                                                                                                 <  2:2372716/101‑1 (MQ=255)
          tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                                >  1:6125087/1‑101 (MQ=255)
           cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                                               >  2:4326089/1‑101 (MQ=255)
             aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                             >  2:2405396/1‑101 (MQ=255)
             aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                             >  1:7033111/1‑101 (MQ=255)
              aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcaccaa                                                                                            >  2:5992405/1‑100 (MQ=255)
               aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                           <  1:1758260/101‑1 (MQ=255)
                ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                          >  2:5517244/1‑101 (MQ=255)
                  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...Gt                                                                                     >  2:3206767/1‑101 (MQ=255)
                  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...Gt                                                                                     >  1:3342470/1‑101 (MQ=255)
                  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGACCTGCACCACCG...Gt                                                                                     <  1:4189578/101‑1 (MQ=255)
                   ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTc                                                                                    >  1:2866665/1‑101 (MQ=255)
                   ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCGGCACCACCG...GTc                                                                                    <  1:6356429/101‑1 (MQ=255)
                     cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGc                                                                                  >  1:5127065/1‑101 (MQ=255)
                      aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCt                                                                                 >  1:6825502/1‑101 (MQ=255)
                      aaTGGACATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCt                                                                                 <  2:758771/101‑1 (MQ=255)
                       aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.c                                                                               >  1:361845/1‑101 (MQ=255)
                        tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.cc                                                                              <  1:5783717/101‑1 (MQ=255)
                         ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.ccc                                                                             >  1:1454355/1‑101 (MQ=255)
                           atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.ccccc                                                                           <  2:7051313/101‑1 (MQ=255)
                             atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcg                                                                         >  2:5846690/1‑101 (MQ=255)
                               ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcgcg                                                                       >  2:4870127/1‑101 (MQ=255)
                               ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcgcg                                                                       >  1:392166/1‑101 (MQ=255)
                                 cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCggg                                                                     >  1:5973596/1‑101 (MQ=255)
                                  aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCgggg                                                                    >  1:1242215/1‑101 (MQ=255)
                                  aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCgggg                                                                    <  1:3578413/101‑1 (MQ=255)
                                   aaaACGTCCGCGACATGTCGCTCCCCCCGCGGGGGGGGGGATCGGAAAACCG.TCATC.ACCACGCAA.ACGCCCCGCACCACCC...GGCGCC.CCCCCCCCcgggg                                                                   >  2:3775184/1‑101 (MQ=255)
                                    aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGc                                                                  <  1:10128/101‑1 (MQ=255)
                                    aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGc                                                                  >  1:4000109/1‑101 (MQ=255)
                                      ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGt                                                                <  2:6103343/101‑1 (MQ=255)
                                       cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTg                                                               <  2:1212178/101‑1 (MQ=255)
                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                              >  1:1813906/1‑101 (MQ=255)
                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                              >  1:210091/1‑101 (MQ=255)
                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                              >  2:4028131/1‑101 (MQ=255)
                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                              <  2:1607763/101‑1 (MQ=255)
                                           gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGa                                                             >  1:2674346/9‑101 (MQ=0)
                                           gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGa                                                             >  2:3649821/9‑101 (MQ=0)
                                           cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATc                                                           >  2:2534000/1‑101 (MQ=255)
                                            gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                          <  2:5434877/101‑1 (MQ=255)
                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                        >  2:6342075/1‑101 (MQ=255)
                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                        <  2:1912911/101‑1 (MQ=255)
                                              cccgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                          <  2:4105243/96‑1 (MQ=2)
                                              accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGGGGAtag                                                          >  2:2979050/6‑99 (MQ=0)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacg                                                     >  2:3945302/1‑100 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacaac                                                   >  1:3115698/1‑101 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.TGCGC.GCCATGAAT.TCATCGAGCACCATCGAGCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAaaca                                                     >  2:1753864/1‑101 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGGGGATCGAaacggtgtc                                                >  1:4403783/1‑95 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                               <  1:162487/101‑8 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.ACCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                               >  1:4865185/1‑94 (MQ=2)
                                                          cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                              <  2:4452985/101‑9 (MQ=2)
                                                          cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGAGCGTGGATCGAaacggtgtcca                                              >  1:327743/1‑93 (MQ=2)
                                                           ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                           <  2:6268954/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGATACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGCAACAACCCGTTAATg                                          <  1:5627054/101‑1 (MQ=38)
                                                              cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                        <  2:2745487/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                      >  2:5341879/1‑101 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                      >  1:876238/1‑101 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                      <  2:2026408/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                      <  2:4558488/101‑1 (MQ=255)
                                                                    ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcgt                                  >  1:1536991/1‑99 (MQ=255)
                                                                    ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                  <  2:4646524/101‑1 (MQ=255)
                                                                     gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                 >  2:2194936/1‑101 (MQ=255)
                                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                <  2:1152650/101‑1 (MQ=255)
                                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                >  1:1160417/1‑101 (MQ=255)
                                                                       gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                               >  1:6506329/1‑101 (MQ=255)
                                                                         ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                             >  2:288276/1‑101 (MQ=255)
                                                                         ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                             >  2:3251479/1‑101 (MQ=255)
                                                                         ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCACGGCCCGCCTCAgcg                             <  2:1383374/101‑1 (MQ=255)
                                                                          gATCGAAACACCG.TCATC.ACCACGCAA.CGGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGCGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                            >  1:6283762/1‑101 (MQ=255)
                                                                          gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                            <  2:3049428/101‑1 (MQ=255)
                                                                           aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                           <  1:6639031/101‑1 (MQ=255)
                                                                           aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                           >  1:5395714/1‑101 (MQ=255)
                                                                             cGAAACACCG.TCATC.CCGACGCAA.CCGCCCTGCACCACCG...GACGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCAGATCATGGCCCGCCGCAGcgccgc                         >  2:5671130/1‑101 (MQ=38)
                                                                             cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                         <  2:3468031/101‑1 (MQ=255)
                                                                              gAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                        >  2:6779249/1‑101 (MQ=255)
                                                                                 acacCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTg                     >  2:418906/1‑101 (MQ=255)
                                                                                     cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                 <  1:2470117/101‑1 (MQ=255)
                                                                                      g.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                <  1:6906728/101‑1 (MQ=255)
                                                                                      g.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                <  1:2607001/101‑1 (MQ=255)
                                                                                      g.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                >  1:4684006/1‑101 (MQ=255)
                                                                                        tcatc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCg               >  1:7131550/1‑101 (MQ=255)
                                                                                         catc.aCCACGCAC.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc              >  1:5078247/1‑101 (MQ=255)
                                                                                         catc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc              >  1:989294/1‑101 (MQ=255)
                                                                                          atc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTAGCt             >  2:2267810/1‑101 (MQ=255)
                                                                                             ccccacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc           <  2:508273/99‑1 (MQ=255)
                                                                                              cccccGCAA.CCGCCCTGCCCCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc          <  2:4802018/97‑1 (MQ=255)
                                                                                              cccacGCAA.CCGCCCTGCACCACCG...GTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGCCGCTcc           <  1:5517534/100‑1 (MQ=255)
                                                                                                cacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc        <  2:385467/101‑1 (MQ=255)
                                                                                                cacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc        >  1:3512865/1‑101 (MQ=255)
                                                                                                 acGCAA.CCGCCCTGCCCCGCCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc       <  1:2264302/101‑1 (MQ=255)
                                                                                                    cAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg    <  2:6447937/101‑1 (MQ=255)
                                                                                                     aa.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCgg   >  1:2018806/1‑101 (MQ=255)
                                                                                                       |                                                                                                        
GGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGG  >  NC_014034/1397383‑1397583

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG