Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,381 +G 15.9% intergenic (‑160/‑798) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3812.→G15.9% 231.7 ‑0.4 ‑0.2439intergenic (‑160/‑798)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (31/39):  ref base (176/193):  total (207/232)
Fisher's exact test strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.99e-01

Only 100 of 460 total aligned reads displayed.

ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAG  >  NC_014034/1434281‑1434481
                                                                                                     |                                                                                                         
aTGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                            >  1:6543757/1‑101 (MQ=255)
 tGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..g                                                                                                         <  1:6329910/101‑1 (MQ=255)
   gggTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtc                                                                                                       >  2:1396331/1‑101 (MQ=255)
      tataTGCACCGCTTACCGTGGGTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                    <  1:2208821/101‑1 (MQ=255)
      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                    <  2:4792275/101‑1 (MQ=255)
      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                    <  1:4184516/101‑1 (MQ=255)
      tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                    <  2:389230/101‑1 (MQ=255)
       atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCg                                                                                                   >  1:7122873/1‑101 (MQ=255)
       atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCg                                                                                                   <  2:6244499/101‑1 (MQ=255)
          tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctc                                                                                               >  2:1141820/1‑101 (MQ=255)
                gcgtACAGTGGATCGAAACAGGCTGCTTTAGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                        <  2:2769436/98‑1 (MQ=255)
                gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                        <  2:2024821/101‑1 (MQ=255)
                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                      <  2:302093/101‑1 (MQ=255)
                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                      >  2:1644450/1‑101 (MQ=255)
                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                      <  2:6191022/101‑1 (MQ=255)
                  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                      >  2:2262970/1‑101 (MQ=255)
                    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACg.g                                                                                   >  2:4907801/1‑101 (MQ=255)
                      cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                 >  1:5671446/1‑101 (MQ=255)
                       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                >  1:1368280/1‑101 (MQ=255)
                        tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.c                                                                              >  2:2437170/1‑101 (MQ=255)
                         ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.Cg                                                                             >  2:2066066/1‑101 (MQ=255)
                          gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                            >  2:5436943/1‑101 (MQ=255)
                           aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTc                                                                           >  2:3702677/1‑101 (MQ=255)
                            tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCa                                                                          <  1:6057020/101‑1 (MQ=255)
                             cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCaa                                                                         <  2:5007392/101‑1 (MQ=255)
                              gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCCAc                                                                        >  2:966617/1‑101 (MQ=255)
                              gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                        <  1:323162/101‑1 (MQ=255)
                              gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                        >  2:4324066/1‑101 (MQ=255)
                               gaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                       <  2:6956184/100‑1 (MQ=255)
                               aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                       <  2:2816395/101‑1 (MQ=255)
                                aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                      >  1:369489/1‑101 (MQ=255)
                                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                     <  1:2751098/101‑1 (MQ=255)
                                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                     <  1:3017210/101‑1 (MQ=255)
                                  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                    <  1:482311/101‑1 (MQ=255)
                                   aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGt                                                                   <  2:6482037/101‑1 (MQ=255)
                                     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCg                                                                 >  2:2603395/1‑101 (MQ=255)
                                     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCg                                                                 >  1:5595044/1‑101 (MQ=255)
                                     gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAAG.GCTG.CGTCAACCTTGTCg                                                                 >  2:5301108/1‑101 (MQ=255)
                                      ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGc                                                                <  1:1926842/101‑1 (MQ=255)
                                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccc                                                            >  1:7015105/1‑101 (MQ=255)
                                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                          >  2:3780074/1‑101 (MQ=255)
                                                   aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                   >  2:6683170/1‑101 (MQ=255)
                                                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                <  1:2610468/101‑1 (MQ=255)
                                                           gtCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:7106054/100‑1 (MQ=255)
                                                           gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                              <  1:829676/91‑1 (MQ=2)
                                                             gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:4183652/9‑101 (MQ=2)
                                                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:6883974/1‑101 (MQ=255)
                                                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGag                                           >  1:315830/8‑100 (MQ=2)
                                                                 tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  1:6760724/5‑101 (MQ=2)
                                                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                     <  1:6475090/101‑1 (MQ=255)
                                                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                     <  2:1894252/101‑1 (MQ=255)
                                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                    >  1:1642815/1‑100 (MQ=255)
                                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                    >  1:3511915/1‑101 (MQ=255)
                                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                    >  2:2888078/1‑101 (MQ=255)
                                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                       >  1:3867631/4‑101 (MQ=2)
                                                                   tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:1821071/99‑1 (MQ=2)
                                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                   <  1:4793256/101‑1 (MQ=255)
                                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                   >  1:5097329/1‑101 (MQ=255)
                                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                   <  1:3586684/101‑1 (MQ=255)
                                                                   gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAacac                                   >  1:4492464/1‑101 (MQ=255)
                                                                   gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACCCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:5003609/99‑1 (MQ=2)
                                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                     <  2:5083996/100‑1 (MQ=2)
                                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                     <  1:4269048/100‑1 (MQ=2)
                                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                     <  2:5966639/100‑1 (MQ=2)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                  <  1:985333/101‑4 (MQ=2)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                               >  2:6103555/1‑101 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              <  1:5430169/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              <  2:4430132/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                              <  1:2532258/101‑1 (MQ=255)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                >  2:5114667/1‑96 (MQ=2)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                             >  2:6389878/1‑101 (MQ=255)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                             <  2:5671156/101‑1 (MQ=255)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                               <  2:5783081/101‑7 (MQ=2)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                            <  1:2055830/101‑1 (MQ=255)
                                                                          cccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                               <  2:1549792/100‑7 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGTGTGGATCGAAacgctgctg                              <  2:3255055/101‑8 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                           >  2:6570723/1‑101 (MQ=255)
                                                                           ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                           >  2:524063/1‑101 (MQ=255)
                                                                            cccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                             <  1:2841235/101‑9 (MQ=2)
                                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                           <  1:4411515/101‑11 (MQ=0)
                                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                           <  1:5299602/101‑11 (MQ=0)
                                                                               ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                       <  1:4294939/101‑1 (MQ=255)
                                                                               ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                       <  2:744805/101‑1 (MQ=255)
                                                                                cgcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                      <  1:5032315/101‑1 (MQ=255)
                                                                                 gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTC.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                     >  2:3455053/1‑101 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                    <  1:3814164/101‑1 (MQ=255)
                                                                                     gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                 >  2:4096684/1‑101 (MQ=255)
                                                                                     gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                 >  2:3145628/1‑101 (MQ=255)
                                                                                     gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                 >  2:1758530/1‑101 (MQ=255)
                                                                                        cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCAGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa              <  1:6673968/101‑1 (MQ=255)
                                                                                         gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc             <  1:1009773/101‑1 (MQ=255)
                                                                                          tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa            <  1:5819563/101‑1 (MQ=255)
                                                                                               cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt       <  1:6146444/101‑1 (MQ=255)
                                                                                               cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt       >  2:3590540/1‑101 (MQ=255)
                                                                                               cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt       >  1:1554035/1‑101 (MQ=255)
                                                                                                gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt      >  1:888368/1‑101 (MQ=255)
                                                                                                 aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg     <  1:6238234/101‑1 (MQ=255)
                                                                                                  aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc    >  1:2337575/1‑101 (MQ=255)
                                                                                                  aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc    <  1:1141868/101‑1 (MQ=255)
                                                                                                   aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa   >  2:6844019/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
ATGGGGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAG  >  NC_014034/1434281‑1434481

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG