Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,414 +A 0.8% intergenic (‑193/‑765) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4142.→A 0.8% 3.7 0.0 ‑0.0396intergenic (‑193/‑765)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (173/220):  total (174/222)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.15e-01

Only 100 of 407 total aligned reads displayed.

ACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434314‑1434510
                                                                                                                                                                                                           
  aGGCTGCTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGt                                                                                                <  2:3436997/101‑1 (MQ=255)
   ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTc                                                                                               <  1:2804939/101‑1 (MQ=255)
     ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGc                                                                                             <  1:1926842/101‑1 (MQ=255)
          ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccc                                                                                        <  2:6569046/101‑1 (MQ=255)
           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                                                       >  2:3780074/1‑101 (MQ=255)
           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                                                       <  1:5531817/101‑1 (MQ=255)
             ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCcg                                                                                     <  1:705157/101‑1 (MQ=255)
                ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgg                                                                                  >  1:6141915/1‑101 (MQ=255)
                  aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                >  2:6475435/1‑101 (MQ=255)
                  aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                <  2:934304/101‑1 (MQ=255)
                   acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggggg                                                                               >  2:1590042/1‑101 (MQ=255)
                    cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                              <  2:2123587/101‑1 (MQ=255)
                    cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                              <  1:6475829/101‑1 (MQ=255)
                       cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:673571/101‑1 (MQ=255)
                       cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  1:384804/1‑101 (MQ=255)
                       cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:1250495/101‑1 (MQ=255)
                        tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGGgg                                                                          >  1:4546577/1‑101 (MQ=255)
                         gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:5237209/1‑101 (MQ=255)
                          gtCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:7106054/100‑1 (MQ=255)
                           tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:6803676/101‑1 (MQ=255)
                            gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  2:4183652/9‑101 (MQ=2)
                            ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:116341/101‑1 (MQ=255)
                             ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  1:3238747/1‑101 (MQ=255)
                               gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:898962/101‑1 (MQ=255)
                               gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:3620328/1‑101 (MQ=255)
                               gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:6948446/1‑101 (MQ=255)
                               ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:719225/6‑101 (MQ=2)
                               ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  2:2682317/6‑101 (MQ=2)
                                tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:6789447/97‑1 (MQ=2)
                                ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:1894252/101‑1 (MQ=255)
                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  2:2888078/1‑101 (MQ=255)
                                 atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:2985970/4‑101 (MQ=2)
                                  gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  1:1682638/1‑101 (MQ=255)
                                  gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  2:1498930/1‑101 (MQ=255)
                                  gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACCCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:5003609/99‑1 (MQ=2)
                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:4269048/100‑1 (MQ=2)
                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:5083996/100‑1 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                                                >  2:6297963/1‑99 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                             <  2:3538276/101‑1 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                            >  1:99368/1‑101 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                            >  2:6103555/1‑101 (MQ=255)
                                      cGCTCCCCCCCCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACACGGAt                                                            <  1:5080372/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGTATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGCAtt                                                           <  1:4362406/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  1:2532258/101‑1 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                             >  2:1757276/1‑96 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                          <  2:5671156/101‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            <  2:703277/101‑7 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            <  1:3137469/101‑7 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATAGatcggaagag                                                            >  2:5121922/1‑92 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                         <  1:862636/101‑1 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            <  2:4995827/100‑7 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  1:2144518/1‑94 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  1:2582013/1‑94 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  2:2788609/1‑94 (MQ=2)
                                          ccccccGCGGGGGCGCGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           <  2:6621693/101‑8 (MQ=0)
                                            ccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                         <  1:2682413/101‑10 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                         <  1:4183847/101‑10 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                         <  2:2313384/101‑10 (MQ=2)
                                              ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  2:744805/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  1:4294939/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                                  >  2:5881235/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 >  1:4457251/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 >  1:2017044/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:4814754/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:6186440/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:4096684/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:3145628/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                                             <  2:3882484/101‑1 (MQ=255)
                                                        gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          >  2:3214461/1‑101 (MQ=255)
                                                        gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          >  1:1572355/1‑101 (MQ=255)
                                                        gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          <  1:1009773/101‑1 (MQ=255)
                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  1:2263065/101‑1 (MQ=255)
                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         >  2:2018148/1‑101 (MQ=255)
                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  1:5819563/101‑1 (MQ=255)
                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         >  2:5284857/1‑101 (MQ=255)
                                                          ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        <  1:5909661/101‑1 (MQ=255)
                                                           gATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                                       >  1:4862087/1‑101 (MQ=255)
                                                             tCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                     >  2:6220441/1‑101 (MQ=255)
                                                             tCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                     >  1:3538084/1‑101 (MQ=255)
                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  2:5366881/1‑101 (MQ=255)
                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  2:2523626/101‑1 (MQ=255)
                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  1:1369623/1‑101 (MQ=255)
                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:560352/101‑1 (MQ=255)
                                                                aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  >  2:4361934/1‑101 (MQ=255)
                                                                 aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  1:6301926/101‑1 (MQ=255)
                                                                 aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  1:6667307/101‑1 (MQ=255)
                                                                  aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                >  2:6844019/1‑101 (MQ=255)
                                                                          ccG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                          <  2:4461709/101‑1 (MQ=255)
                                                                           cG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         <  1:4751847/101‑1 (MQ=255)
                                                                                 tG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGCCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  1:3706436/101‑1 (MQ=255)
                                                                                  g.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  1:2888187/101‑1 (MQ=255)
                                                                                  g.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  2:6525053/101‑1 (MQ=255)
                                                                                  g.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   >  2:6692241/1‑101 (MQ=255)
                                                                                     ttCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  2:3358562/1‑101 (MQ=255)
                                                                                            gCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg           >  1:5330902/1‑101 (MQ=40)
                                                                                               g.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  1:5437215/101‑1 (MQ=37)
                                                                                                 cGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:5237475/101‑1 (MQ=35)
                                                                                                    cAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:3434363/1‑101 (MQ=32)
                                                                                                     aaCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  2:1642921/101‑1 (MQ=31)
                                                                                                      aCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:3924600/101‑1 (MQ=11)
                                                                                                                                                                                                           
ACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434314‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG