Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,614,014 +C 1.3% intergenic (+177/+50) rnd2 → / ← uvrD ribonuclease D/DNA helicase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,614,0141.→C 1.3% 4.2 ‑2.1 ‑0.4300intergenic (+177/+50)rnd2/uvrDribonuclease D/DNA helicase II
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (149/147):  total (149/151)
Fisher's exact test strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.63e-01

Only 100 of 318 total aligned reads displayed.

GGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCTT  >  NC_014034/1613914‑1614114
                                                                                                      |                                                                                                    
  ctgccccccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.At                                                                                                    <  1:6331297/95‑1 (MQ=255)
   ccgcgcCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATg                                                                                                   <  1:5981216/99‑1 (MQ=255)
   cTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATg                                                                                                   <  2:1406364/101‑1 (MQ=255)
    tGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgc                                                                                                  <  2:1637479/101‑1 (MQ=255)
        cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgggc                                                                                              <  2:774603/101‑1 (MQ=255)
        cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgggc                                                                                              <  1:1432633/101‑1 (MQ=255)
        cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgggc                                                                                              <  2:3492074/101‑1 (MQ=255)
        cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgggc                                                                                              <  1:2418113/101‑1 (MQ=255)
          cgccccgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgggcgg                                                                                            <  1:3527343/101‑1 (MQ=255)
             cccgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGa                                                                                         <  1:1941851/101‑1 (MQ=255)
               cgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGCCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGaaa                                                                                       <  1:5018192/101‑1 (MQ=255)
                gccATCGGCGGGCGCATTCGCGCCGCCGTGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAg                                                                                      <  1:4976017/101‑1 (MQ=255)
                gccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAg                                                                                      <  2:2324684/101‑1 (MQ=255)
                 cccatggcgggcgCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCCCCGGCTTTGGTG.GGGCCGGCggggggag                                                                                      >  2:4378672/6‑97 (MQ=18)
                 ccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTTTGTTGCGCTTTGGGGAACGGTCTTG.CCGCGGCTGTTGCG.GTGCGGGCGGGGGAAgg                                                                                     >  2:7081268/1‑101 (MQ=18)
                 ccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCCCGGCTGTTGCG.ATGCTGGCGGGGAAAgg                                                                                     <  1:6273870/101‑1 (MQ=255)
                  cATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGc                                                                                    <  1:440921/101‑1 (MQ=255)
                  cATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGc                                                                                    <  1:4890762/101‑1 (MQ=255)
                     cggcggGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGt                                                                                 <  2:5750713/101‑1 (MQ=255)
                      ggcgggcgCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTg                                                                                <  1:809685/101‑1 (MQ=255)
                          ggcgCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCt                                                                            >  1:719105/1‑101 (MQ=255)
                              cATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCt                                                                        <  1:2848497/101‑1 (MQ=255)
                              cATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCt                                                                        >  2:3866755/1‑101 (MQ=255)
                              cATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCt                                                                        <  2:2658179/101‑1 (MQ=255)
                               aTTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTg                                                                       >  1:268942/1‑101 (MQ=255)
                                ttCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGa                                                                      >  2:6039762/1‑101 (MQ=255)
                                tccgcgCCGCGGGGGGGGGCAAAAGGCCTCTGTCGTGCTTGGGGAAACGGTCTTG.GCACAGGTGATGCGCCAGCGGGCGGGGAAAGGCAGTGCCTTTcccg                                                                       <  1:2035181/99‑3 (MQ=25)
                                   gcgcCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                                                                   <  2:2154596/101‑1 (MQ=255)
                                     gccgccGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                                                                 >  2:4969857/1‑101 (MQ=255)
                                     gccgccGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                                                                 >  2:3631642/1‑101 (MQ=255)
                                      ccgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGcc                                                                <  2:5101236/101‑1 (MQ=255)
                                      ccgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGcc                                                                <  1:6942217/101‑1 (MQ=255)
                                       cgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                                                               <  1:1776872/101‑1 (MQ=255)
                                       cgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                                                               <  1:5479626/101‑1 (MQ=255)
                                        gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                              <  1:867787/101‑1 (MQ=255)
                                        gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                              <  2:5592079/101‑1 (MQ=255)
                                        gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                              >  2:6302267/1‑101 (MQ=255)
                                        gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                              <  2:834243/101‑1 (MQ=255)
                                         ccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGa                                                             >  2:2773634/1‑101 (MQ=255)
                                          cgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGaa                                                            >  1:315519/1‑101 (MQ=255)
                                          cgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGaa                                                            >  2:1173655/1‑101 (MQ=255)
                                           ggggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAGAGGCAGTGCCCTTCCTGATCCGCCCGAAc                                                           >  1:4502289/1‑101 (MQ=255)
                                            gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                          >  2:3732027/1‑101 (MQ=255)
                                            gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                          <  1:3429841/101‑1 (MQ=255)
                                               cGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                       <  2:6782203/101‑1 (MQ=255)
                                               cGGGACGCACGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                       <  1:3284922/101‑1 (MQ=255)
                                                 ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                     >  2:2527650/1‑101 (MQ=255)
                                                 ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                     >  1:1075198/1‑101 (MQ=255)
                                                     gCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg                                                 >  1:3939329/1‑101 (MQ=255)
                                                      cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                >  1:1635144/1‑101 (MQ=255)
                                                      cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                >  2:5836551/1‑101 (MQ=255)
                                                      cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                >  1:5769358/1‑101 (MQ=255)
                                                       aGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa                                               <  1:3178672/101‑1 (MQ=255)
                                                       aGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa                                               >  1:589228/1‑101 (MQ=255)
                                                        ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                              >  2:4565582/1‑101 (MQ=255)
                                                         gCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg                                             <  2:3184118/101‑1 (MQ=255)
                                                           cTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc                                           >  1:5189375/1‑101 (MQ=255)
                                                            tttGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa                                          >  1:1562855/1‑101 (MQ=255)
                                                             ttgttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAc                                         >  2:5187050/1‑101 (MQ=255)
                                                              tgttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcg                                        >  2:3201458/1‑101 (MQ=255)
                                                               gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                       >  2:2385768/1‑101 (MQ=255)
                                                               gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                       <  2:3065717/101‑1 (MQ=255)
                                                               gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                       >  2:4843928/1‑101 (MQ=255)
                                                               gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgc                                       >  2:6393736/1‑100 (MQ=255)
                                                                    gcTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTcc                                  <  1:1532991/101‑1 (MQ=255)
                                                                     cTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                 <  2:2008701/101‑1 (MQ=255)
                                                                     cTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                 <  1:1152250/101‑1 (MQ=255)
                                                                      ttGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAg                                >  1:2153025/1‑101 (MQ=255)
                                                                       tGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGc                               >  2:2489000/1‑101 (MQ=255)
                                                                        ggggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGGAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGGACGGCACGTGGTCCCGcc                              >  2:2234681/1‑101 (MQ=21)
                                                                        ggggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGcc                              >  2:6572565/1‑101 (MQ=255)
                                                                        ggggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGcc                              >  2:1743340/1‑101 (MQ=255)
                                                                        ggggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGcc                              <  1:2738089/101‑1 (MQ=255)
                                                                         gggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGccc                             <  2:4652972/101‑1 (MQ=255)
                                                                             aaCGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcg                         >  1:2560091/1‑101 (MQ=255)
                                                                             aaCGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcg                         >  2:5295311/1‑101 (MQ=255)
                                                                               cGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                       <  1:5261043/101‑1 (MQ=255)
                                                                               cGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                       <  1:1376331/101‑1 (MQ=255)
                                                                                ggTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggcg                      >  2:4790054/1‑101 (MQ=255)
                                                                                  tCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCggg                    >  1:6456406/1‑101 (MQ=255)
                                                                                   cTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGt                   >  2:918337/1‑101 (MQ=255)
                                                                                    ttG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGtt                  <  1:1337638/101‑1 (MQ=255)
                                                                                     tG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGttt                 <  2:1937726/101‑1 (MQ=255)
                                                                                     tG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGttt                 >  1:5585062/1‑101 (MQ=255)
                                                                                      g.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTc                <  2:5057018/101‑1 (MQ=255)
                                                                                        ccGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa               <  2:572529/101‑1 (MQ=255)
                                                                                        ccGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa               <  1:3752896/101‑1 (MQ=255)
                                                                                         cgcgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAg              <  1:6636453/101‑1 (MQ=255)
                                                                                          gcgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAgg             <  2:6223195/101‑1 (MQ=255)
                                                                                           cgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGa            >  1:5805797/1‑101 (MQ=255)
                                                                                            ggCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGaa           >  1:4299129/1‑101 (MQ=255)
                                                                                              cTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAcc         <  2:4200072/101‑1 (MQ=255)
                                                                                               tGTTGCG.ATTCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAccc        >  1:5382209/1‑101 (MQ=255)
                                                                                               tGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAAccc        >  1:975779/1‑101 (MQ=255)
                                                                                               tGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAAccc        >  1:6809412/1‑101 (MQ=255)
                                                                                                  tGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAACCCGGc     >  1:4051242/1‑101 (MQ=255)
                                                                                                    cG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCt   <  2:4080340/101‑1 (MQ=255)
                                                                                                    cG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCt   <  2:5425930/101‑1 (MQ=255)
                                                                                                    cG.ATCCCGGCGGGGAAACGCAGTGCCCGTCCTGATCCGCGCGAACCCGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCt   <  2:6930761/101‑1 (MQ=255)
                                                                                                     g.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGCTTCAGGAACCCGGCCtt  >  2:5643319/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCTT  >  NC_014034/1613914‑1614114

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG