Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,177 +C 92.7% intergenic (‑105/+120) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1771.→C92.7% 74.6 ‑1.1 ‑1.6368intergenic (‑105/+120)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (165/176):  ref base (16/11):  total (181/187)
Fisher's exact test strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.58e-02

Only 100 of 395 total aligned reads displayed.

GCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGC  >  NC_014034/1696075‑1696276
                                                                                                       |                                                                                                    
gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCgg                                                                                                       >  1:1651226/1‑101 (MQ=255)
  tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.cc                                                                                                    >  2:4673267/1‑100 (MQ=255)
   cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.cca                                                                                                   <  1:48206/101‑3 (MQ=255)
   cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.cca                                                                                                   <  2:1007923/101‑3 (MQ=255)
   cctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.cca                                                                                                   <  2:4915306/101‑3 (MQ=255)
   cctccAAAAGTGCCTGAATTGAACGTCTGTCTAACTACGAATCCTTGCACCAGTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.cca                                                                                                   <  2:5885917/101‑3 (MQ=255)
    ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.ccaa                                                                                                  >  1:7119664/1‑98 (MQ=255)
       ccAAAGTGCCTGGATTGAACGGCTGTTTAAGTTCGAATCCTTGCACGAATGTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTc                                                                                                <  1:7009135/101‑1 (MQ=255)
       ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTc                                                                                                >  1:3898638/1‑101 (MQ=255)
        cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTca                                                                                               >  2:3812334/1‑100 (MQ=255)
        cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTca                                                                                               <  2:718586/101‑2 (MQ=255)
         aaaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTcac                                                                                              <  2:3595689/101‑3 (MQ=255)
          aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCA.cc                                                                                           <  1:6262574/101‑1 (MQ=255)
            gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCA.cccc                                                                                         >  2:5659132/1‑100 (MQ=255)
              gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCA.CCCcgg                                                                                       <  1:4670649/101‑1 (MQ=255)
              gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCA.CCCcgg                                                                                       >  1:5647293/1‑101 (MQ=37)
                 tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGan                                                                                     >  1:6392415/1‑100 (MQ=37)
                 tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCACTTCA.CCCcgggat                                                                                    >  2:2766800/1‑98 (MQ=25)
                     ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGatgttt                                                                                 >  1:5738109/1‑96 (MQ=255)
                     ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGatgttt                                                                                 <  1:6195232/101‑6 (MQ=25)
                       gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTgg                                                                               <  1:6634658/101‑1 (MQ=255)
                       gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTgg                                                                               >  1:1965379/1‑101 (MQ=25)
                       gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTgg                                                                               >  2:6899964/1‑101 (MQ=25)
                         aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGc                                                                             >  2:4148034/1‑101 (MQ=255)
                           gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                           >  2:1113242/1‑101 (MQ=255)
                           gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                           <  2:3199618/101‑1 (MQ=25)
                           gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCGTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                           >  2:5253111/1‑101 (MQ=255)
                            tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaa                                                                          >  1:5846125/1‑101 (MQ=255)
                             cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                         <  2:5511460/101‑2 (MQ=255)
                              tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                        >  1:904090/1‑99 (MQ=255)
                              tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                        >  2:1524776/1‑99 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                       >  1:1014122/1‑98 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                       >  2:372922/1‑98 (MQ=255)
                               gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                       >  1:4130634/1‑98 (MQ=255)
                                tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                      >  2:1427762/1‑97 (MQ=19)
                                tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                      <  1:6135653/101‑5 (MQ=255)
                                tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                      >  2:786928/1‑97 (MQ=19)
                                tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTAAC.CCCGGGATGTTTGGGCCaagctg                                                                      >  1:4044957/1‑97 (MQ=19)
                                 ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCCC.CCCGGGATGTTTGGGCCaaggtga                                                                     <  2:4059024/101‑6 (MQ=18)
                                 ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                     <  2:2676532/101‑6 (MQ=16)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                    <  1:4910130/101‑7 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                    <  2:5148971/101‑7 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                    >  1:2859564/1‑95 (MQ=255)
                                  tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCATTTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                    <  2:1657252/101‑7 (MQ=12)
                                   aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                   >  2:2015217/1‑94 (MQ=16)
                                   aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                   >  1:1810263/1‑94 (MQ=16)
                                    aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                <  1:3224680/101‑1 (MQ=16)
                                      ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                              >  1:2912908/1‑99 (MQ=12)
                                      ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                              >  2:4200366/1‑99 (MQ=12)
                                       tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                             <  2:4503532/101‑4 (MQ=12)
                                        cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                            <  1:177470/101‑5 (MQ=255)
                                         gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                           >  1:1055208/1‑96 (MQ=12)
                                         gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                           <  2:1789753/101‑6 (MQ=255)
                                           aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                         <  2:1278717/101‑8 (MQ=255)
                                           aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                         >  1:146185/1‑94 (MQ=255)
                                             ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                       >  1:1552018/1‑92 (MQ=255)
                                             ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                       <  2:3000454/101‑10 (MQ=9)
                                             ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                       <  2:1856816/101‑10 (MQ=9)
                                                  cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                <  1:4461651/101‑1 (MQ=255)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                               <  2:258116/101‑1 (MQ=9)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                               <  2:4812976/101‑1 (MQ=9)
                                                   aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                               <  1:2227602/101‑1 (MQ=9)
                                                    cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                              <  2:2430718/101‑1 (MQ=255)
                                                     gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                             >  1:5537181/1‑101 (MQ=9)
                                                         tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                         >  1:4950128/1‑101 (MQ=255)
                                                          ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                        <  1:2712860/101‑1 (MQ=255)
                                                          ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                        >  2:221816/1‑101 (MQ=255)
                                                          ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                        >  1:2166478/1‑101 (MQ=255)
                                                            gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                      <  1:1278188/101‑1 (MQ=9)
                                                            gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                      <  1:6900318/101‑1 (MQ=9)
                                                            gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                      <  2:3222771/101‑1 (MQ=9)
                                                             cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                     <  1:5265399/101‑1 (MQ=255)
                                                              aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                    <  2:1601832/101‑1 (MQ=255)
                                                               aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                   <  2:6718758/101‑1 (MQ=9)
                                                               aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                   >  1:3758197/1‑101 (MQ=255)
                                                                ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                  <  2:6378909/101‑1 (MQ=255)
                                                                ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                  <  1:472323/101‑1 (MQ=255)
                                                                 gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                 >  1:3365817/1‑101 (MQ=9)
                                                                   ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                               >  2:615866/1‑101 (MQ=12)
                                                                    gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                              <  2:5072329/101‑1 (MQ=255)
                                                                         agaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                           >  2:6032570/11‑101 (MQ=9)
                                                                          gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                          >  2:4754991/10‑101 (MQ=16)
                                                                           aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                         >  1:5381011/9‑101 (MQ=12)
                                                                            ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                        <  1:1145609/94‑1 (MQ=255)
                                                                            ggggcgctGCACCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                        <  1:5078984/94‑1 (MQ=255)
                                                                               gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                     <  2:2354298/97‑1 (MQ=255)
                                                                                 gctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                   >  1:3612692/3‑101 (MQ=16)
                                                                                    gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                >  2:3503464/1‑101 (MQ=19)
                                                                                    gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                <  2:5752235/101‑1 (MQ=19)
                                                                                       ccTCCTGGCCTTGCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc             <  2:2874219/101‑1 (MQ=16)
                                                                                        cTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg            <  2:656112/101‑1 (MQ=19)
                                                                                            tGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc        >  1:2414465/1‑101 (MQ=255)
                                                                                             ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg       >  2:4358918/1‑101 (MQ=255)
                                                                                             ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg       >  2:2921575/1‑101 (MQ=255)
                                                                                              gCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCACGCCGCGCCgc      >  1:2292141/1‑100 (MQ=255)
                                                                                               ccTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa     >  1:3095794/1‑101 (MQ=255)
                                                                                                cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg    >  2:958249/1‑101 (MQ=255)
                                                                                                cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg    <  1:4252521/101‑1 (MQ=19)
                                                                                                  ttCGGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc  <  1:2810206/101‑1 (MQ=25)
                                                                                                  ttCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc  <  2:1055173/101‑1 (MQ=19)
                                                                                                       |                                                                                                    
GCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGC  >  NC_014034/1696075‑1696276

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG