Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,917 +A 1.5% intergenic (‑170/+23) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,9171.→A 1.5% 10.9 ‑2.7 ‑0.2336intergenic (‑170/+23)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (183/148):  total (188/148)
Fisher's exact test strand distribution p-value = 6.95e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.19e-01

Only 100 of 369 total aligned reads displayed.

GACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTCGGT  >  NC_014034/1734818‑1735017
                                                                                                      |                                                                                                    
gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                                       <  1:5503760/101‑1 (MQ=255)
gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                                       <  1:6284748/101‑1 (MQ=255)
 gcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                                     <  2:5106193/100‑1 (MQ=255)
    ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                                                  >  1:4127672/1‑101 (MQ=255)
     tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                                                >  2:6542554/9‑101 (MQ=2)
      ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                                                >  1:3651629/1‑101 (MQ=255)
      ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                                                <  1:6792831/101‑1 (MQ=255)
       cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                               <  2:1533982/101‑1 (MQ=255)
       acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                                              >  2:4536527/7‑99 (MQ=2)
        cggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                                             >  1:4349599/6‑98 (MQ=2)
           gaGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                                                           <  1:5308266/100‑1 (MQ=255)
           cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                                                           <  1:2486505/101‑1 (MQ=255)
            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCGCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGAGCGGGGc                                                                                          <  2:7081224/101‑1 (MQ=17)
            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGCTGCGGGGc                                                                                          >  1:3307709/1‑101 (MQ=255)
             ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                                         <  2:5398582/101‑1 (MQ=255)
             ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                                         >  2:2357502/1‑101 (MQ=255)
               ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                                                       <  1:449014/101‑1 (MQ=255)
                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCGTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcag                                                                                   >  1:743191/1‑99 (MQ=255)
                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                                                   >  2:631181/1‑101 (MQ=255)
                    gtgg.CCCCCGTTCTGCGGACCCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                                 <  2:2190466/98‑1 (MQ=255)
                    gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                                  >  1:7001833/6‑101 (MQ=255)
                    gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                                  >  1:4311078/6‑101 (MQ=255)
                       gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                               <  1:1790270/99‑1 (MQ=255)
                       gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                               <  2:3067296/99‑1 (MQ=255)
                        ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                                              <  1:4949540/100‑1 (MQ=255)
                         cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                             <  2:884048/101‑1 (MQ=255)
                            ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                                          <  2:6824629/101‑1 (MQ=255)
                             cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                                         <  2:1734063/101‑1 (MQ=255)
                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        >  2:714239/1‑101 (MQ=255)
                               tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                                       >  1:970283/1‑101 (MQ=255)
                                ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                                                      >  2:6446578/1‑101 (MQ=255)
                                  tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                                                    >  1:131277/1‑101 (MQ=255)
                                   gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                                                   <  1:855291/101‑1 (MQ=255)
                                    cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                                  >  1:1036477/1‑101 (MQ=255)
                                     ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                                 >  2:4907478/1‑101 (MQ=255)
                                     ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                                 >  2:2666804/1‑101 (MQ=255)
                                      gACTCCCCCCGGGATATTTATGGCAAGATGAATCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                                                <  2:329526/101‑1 (MQ=255)
                                      gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                                                >  2:4121879/1‑101 (MQ=255)
                                       gcTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                                               <  1:1151313/100‑1 (MQ=255)
                                          ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                            <  1:1651951/101‑1 (MQ=255)
                                           cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                           <  2:3719405/101‑1 (MQ=255)
                                                  gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                                    >  1:443999/1‑101 (MQ=255)
                                                   atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                                   >  1:2019414/1‑101 (MQ=255)
                                                    tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                                  >  1:5847727/1‑101 (MQ=255)
                                                     atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                                                 >  1:3831748/1‑101 (MQ=255)
                                                      tttATGGCAAGATGAAGCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                                <  2:5124737/101‑1 (MQ=255)
                                                      tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                                >  2:4724777/1‑101 (MQ=255)
                                                      tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                                >  1:2769623/1‑101 (MQ=255)
                                                      tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                                >  1:6918291/1‑101 (MQ=255)
                                                       ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                               >  1:4671920/1‑101 (MQ=255)
                                                        tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                              <  1:1328464/101‑1 (MQ=255)
                                                         aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                             <  1:511001/101‑1 (MQ=255)
                                                         aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                             >  2:4348950/1‑101 (MQ=255)
                                                           ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                                           >  2:1087349/1‑101 (MQ=255)
                                                            gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                          >  1:3490581/1‑101 (MQ=255)
                                                             cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                                         >  1:1631014/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  2:5898339/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  1:1896496/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  1:5934203/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  1:4164879/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGCGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  2:5838506/1‑101 (MQ=255)
                                                              aaGATGAAACTGC.GGCCTTCATCTAGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  2:3402552/1‑101 (MQ=255)
                                                               aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga                                       >  2:1970109/1‑101 (MQ=255)
                                                                gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                                      >  2:5764057/1‑101 (MQ=255)
                                                                gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                                      <  1:6519421/101‑1 (MQ=255)
                                                                 aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                                     >  1:2299060/1‑101 (MQ=255)
                                                                 aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAAATGACAAGATCCGCGGCCCGGTTTTCGCcgagg                                     >  2:2260117/1‑99 (MQ=255)
                                                                  tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga                                    >  1:6614373/1‑101 (MQ=255)
                                                                    aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                                  >  1:2880758/1‑101 (MQ=255)
                                                                      aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                                <  1:714259/101‑1 (MQ=255)
                                                                      aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                                <  2:5040016/101‑1 (MQ=255)
                                                                       cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg                               >  2:3596538/1‑101 (MQ=255)
                                                                        tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                              <  2:131274/101‑1 (MQ=255)
                                                                          c.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcgc                            >  1:4763867/1‑101 (MQ=255)
                                                                             gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                          <  2:3848218/101‑1 (MQ=255)
                                                                              ccTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                         >  2:6623013/1‑101 (MQ=255)
                                                                              ccTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg                         >  1:6369806/1‑101 (MQ=255)
                                                                                 tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc                      <  2:4074190/101‑1 (MQ=255)
                                                                                 tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc                      >  2:6202053/1‑101 (MQ=255)
                                                                                  cATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg                     <  1:573314/101‑1 (MQ=255)
                                                                                   aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg                    <  2:3340627/101‑1 (MQ=255)
                                                                                       tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg                >  1:3863290/1‑101 (MQ=255)
                                                                                        gCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt               <  1:3967199/101‑1 (MQ=255)
                                                                                        gCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt               <  1:3112169/101‑1 (MQ=255)
                                                                                         ccctccatacCCC.GGGGTGCGGTGCAGACCACCTCGCAACCGAAGCTGACAAGACCCGCGCCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc              <  1:1525370/95‑1 (MQ=255)
                                                                                          cATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCg             <  2:6995907/101‑1 (MQ=255)
                                                                                            tAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt           >  1:3549057/1‑101 (MQ=255)
                                                                                            tAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt           <  1:1005401/101‑1 (MQ=255)
                                                                                            tAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCCCCCGTTCGGCAGTACGtt           >  2:281059/1‑101 (MQ=255)
                                                                                             aaaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGttt          >  1:5387439/1‑101 (MQ=255)
                                                                                              aaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtttt         <  2:2631063/101‑1 (MQ=255)
                                                                                               atatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTc        >  2:548608/1‑101 (MQ=255)
                                                                                                tatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTcc       >  2:937456/1‑101 (MQ=255)
                                                                                                 atCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCt      <  2:6045060/101‑1 (MQ=255)
                                                                                                 atCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCt      >  2:3508903/1‑101 (MQ=255)
                                                                                                 atCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCt      >  2:318297/1‑101 (MQ=255)
                                                                                                 atCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCt      >  2:122917/1‑101 (MQ=255)
                                                                                                 atCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCt      <  2:6784909/101‑1 (MQ=255)
                                                                                                  tCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTc     <  1:2137191/101‑1 (MQ=255)
                                                                                                   ccc.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGGCGTTTTCCTCg    >  1:4637044/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTCGGT  >  NC_014034/1734818‑1735017

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG