Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,737,122 +A 1.1% intergenic (+63/‑228) RCAP_rcc01606 → / → RCAP_rcc01607 tRNA/rRNA methyltransferase, SpoU family/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,737,1221.→A 1.1% 4.4 ‑1.4 ‑0.0265intergenic (+63/‑228)RCAP_rcc01606/RCAP_rcc01607tRNA/rRNA methyltransferase, SpoU family/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (146/116):  total (149/116)
Fisher's exact test strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.47e-01

Only 100 of 309 total aligned reads displayed.

ACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACGGT  >  NC_014034/1737022‑1737222
                                                                                                      |                                                                                                       
aCGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAcc.gccg                                                                                                          <  1:4606018/101‑1 (MQ=255)
  gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCg.gg                                                                                                       <  2:489279/101‑1 (MQ=255)
  gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCg.gg                                                                                                       >  1:100898/1‑101 (MQ=255)
  gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCg.gg                                                                                                       >  1:1219971/1‑101 (MQ=255)
    gATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCg.gggg                                                                                                     >  2:5541185/1‑101 (MQ=255)
    gATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCg.gggg                                                                                                     <  1:6450530/101‑1 (MQ=255)
     aTGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGc                                                                                                    >  1:4784730/1‑101 (MQ=255)
     aTGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGc                                                                                                    <  1:4061487/101‑1 (MQ=255)
     aTGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGc                                                                                                    >  2:1022822/1‑101 (MQ=255)
     aTGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGc                                                                                                    >  1:2790091/1‑101 (MQ=255)
      tGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGcc                                                                                                   <  1:5512666/101‑1 (MQ=255)
        ttGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCa                                                                                                 <  2:5371913/101‑1 (MQ=255)
        ttGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCa                                                                                                 <  1:5770616/101‑1 (MQ=255)
         tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCaa                                                                                                >  2:191009/1‑101 (MQ=255)
         tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCaa                                                                                                >  2:3554415/1‑101 (MQ=255)
         tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCaa                                                                                                >  1:1517000/1‑101 (MQ=255)
          gCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCaaa                                                                                               >  2:3446774/1‑101 (MQ=255)
           cGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAg                                                                                              <  1:3185919/101‑1 (MQ=255)
             gCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGc                                                                                            >  1:3055062/1‑101 (MQ=255)
              cAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGcc                                                                                           >  2:77705/1‑101 (MQ=255)
               aGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGccc                                                                                          <  2:2473212/101‑1 (MQ=255)
                gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCt                                                                                         >  1:6313542/1‑101 (MQ=255)
                gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCt                                                                                         >  2:4459043/1‑101 (MQ=255)
                gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCt                                                                                         <  2:5811628/101‑1 (MQ=255)
                gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCt                                                                                         <  2:6302450/101‑1 (MQ=255)
                 cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTc                                                                                        <  2:1344270/101‑1 (MQ=255)
                  ttttGCCACCGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCg                                                                                       <  2:2282588/101‑1 (MQ=255)
                       cgcCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCgcacag                                                                                  >  2:154739/1‑96 (MQ=255)
                         ccAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAgcg                                                                                >  1:3241015/1‑101 (MQ=255)
                           aGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGcgcc                                                                              >  2:4809804/1‑101 (MQ=255)
                            gCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                             >  2:3139546/1‑101 (MQ=255)
                            gCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                             <  1:4396356/101‑1 (MQ=255)
                             cGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGcgcccg                                                                            >  2:657900/1‑101 (MQ=255)
                               ggATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGcgcccgcc                                                                          >  2:1091272/1‑101 (MQ=255)
                                 aTTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                                        <  2:5176761/101‑1 (MQ=255)
                                     aGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgc.cc                                                                   >  1:3852863/1‑101 (MQ=255)
                                       gTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAACGGCCCTCGGCCAGCGCCcgccccgc.ccct                                                                 >  1:2313662/1‑99 (MQ=255)
                                       gTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.t                                                                >  1:1073500/1‑101 (MQ=255)
                                       gTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.t                                                                >  2:723053/1‑101 (MQ=255)
                                        cggcagcaccTGACTGAGGCCATCGCCGAGGTCACGGGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tg                                                               <  2:6741235/92‑1 (MQ=14)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggc                                                             >  1:5330340/1‑101 (MQ=255)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggc                                                             <  2:6406342/101‑1 (MQ=255)
                                           aCGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggcg                                                            <  2:5141500/101‑1 (MQ=255)
                                           aCGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggcg                                                            >  2:5723924/1‑101 (MQ=255)
                                            cGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggcgg                                                           >  2:1208121/1‑101 (MQ=255)
                                             ggATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.Tggcggg                                                          >  1:3450394/1‑101 (MQ=255)
                                                tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGgcgc                                                       <  1:7057894/101‑1 (MQ=255)
                                                tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGgcgc                                                       <  2:3662650/101‑1 (MQ=255)
                                                 cggACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCt                                                      <  2:796383/99‑1 (MQ=255)
                                                 cTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCt                                                      >  2:4593262/1‑101 (MQ=255)
                                                 cTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCt                                                      >  2:4089976/1‑101 (MQ=255)
                                                  tGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCtt                                                     <  2:7027832/101‑1 (MQ=255)
                                                   gACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTtc                                                    >  1:2229238/1‑101 (MQ=255)
                                                   gACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCCtc                                                    >  1:6470832/1‑101 (MQ=255)
                                                    aCCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGTCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTtct                                                   <  1:2109693/101‑1 (MQ=255)
                                                     ccGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTtctc                                                  >  2:1713839/1‑101 (MQ=255)
                                                      cGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCCTCTc.g                                                >  1:1785083/1‑101 (MQ=255)
                                                      cGAGGCCACCGCCGAGGTCCCGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.g                                                <  1:837761/101‑1 (MQ=255)
                                                       gAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.gc                                               >  1:5468237/1‑101 (MQ=255)
                                                       gAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.gc                                               >  1:3107834/1‑101 (MQ=255)
                                                        aGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGGGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.gcc                                              >  2:5245681/1‑101 (MQ=255)
                                                            cATTGCCGAGGTCCGGCGCAACAGTGCGGCAAAGACCGGCGG.GGGGCCCAAAGGCCCCCGGGCAGCGCCCGCCCCCC.CCC.CCGCGGGGGCCTCTt.ggccgc                                           >  2:3212896/1‑101 (MQ=21)
                                                            cATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.gcccgcc                                          <  1:2553864/101‑1 (MQ=255)
                                                            cATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTc.gcccgcc                                          <  2:1127412/101‑1 (MQ=255)
                                                               cGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCggg                                       <  2:1659963/101‑1 (MQ=255)
                                                                gCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCgggg                                      >  2:5272752/1‑101 (MQ=255)
                                                                 ccGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGc                                     <  1:4231381/101‑1 (MQ=255)
                                                                  cGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGcc                                    >  1:5209128/1‑100 (MQ=255)
                                                                   gAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGGGCTTCTC.GCCCGCCGGGGCAg                                   >  2:4172511/1‑101 (MQ=255)
                                                                    aGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCCCCCGGGCAgg                                  >  2:4215747/1‑101 (MQ=255)
                                                                     ggTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAggg                                 >  2:5021027/1‑100 (MQ=255)
                                                                        cACGCGCGACATTGGCCCCAGGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGcc                              <  2:2876056/101‑1 (MQ=255)
                                                                         aCGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCg                             <  1:3554551/101‑1 (MQ=255)
                                                                           gcgcgACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCCGGGCAGGCGCCGGc                           >  2:1850616/1‑101 (MQ=255)
                                                                             gcgACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGccc                         >  2:4311889/1‑100 (MQ=255)
                                                                             gcgACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCct                         <  1:4139403/101‑1 (MQ=255)
                                                                               gaaaTTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCctct                       <  1:289864/98‑1 (MQ=255)
                                                                               gACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTTTC.CCCCCCCGGGGGAGGGGCCGGGctcc                       >  1:3297200/1‑100 (MQ=17)
                                                                               gACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCctct                       <  2:3063231/101‑1 (MQ=255)
                                                                                 cATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGt                     <  1:5653844/101‑1 (MQ=255)
                                                                                  aTTTGCCCCAAGACC.GCCGAGGGCCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCGCCACTGGCGGGCGCTTTTCTCCCCCCCCGGGGTGGCGGCGGCctc                        >  2:4648776/1‑101 (MQ=18)
                                                                                  aTTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGGGCTTCTC.GCCCGCCCGGGGGGGGGCCGGCCCCCGtt                    >  1:875036/1‑101 (MQ=14)
                                                                                   ttGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTg                   >  1:2385653/1‑101 (MQ=255)
                                                                                    tGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCCGGGCAGGCGCCGGCCCCTGGTgc                  >  2:3917099/1‑101 (MQ=255)
                                                                                       ccccATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCt               >  2:6235995/1‑101 (MQ=255)
                                                                                        cccATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCCCTGTTGCGCtt              >  2:3237339/1‑101 (MQ=255)
                                                                                         ccATGCCC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTg             <  2:6039488/101‑1 (MQ=255)
                                                                                         ccATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTg             <  1:6112850/101‑1 (MQ=255)
                                                                                          cATGCCC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGa            <  1:508942/101‑1 (MQ=255)
                                                                                          cATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGa            <  1:7095541/101‑1 (MQ=255)
                                                                                           aTGCCC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGAt           <  1:5995856/101‑1 (MQ=255)
                                                                                            tgccc.gccg.GGGGCCAAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATc          <  2:2229143/98‑1 (MQ=255)
                                                                                            ggccc.gccg.GGGGCCCAAAGTCCCTCGGCCAGCCCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATc          <  2:2313567/98‑1 (MQ=255)
                                                                                             gccc.gccg.GGGGCCCAAAGGCCCTCGGCCAGCTCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCg         <  1:4866109/99‑1 (MQ=255)
                                                                                              aCC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCCCTGTTGCGCTTGATCGa        >  1:2589781/1‑101 (MQ=255)
                                                                                                  gccg.GGGGCCAAAAGGCCCTGGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAAc     <  2:2615898/101‑1 (MQ=255)
                                                                                                   ccg.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACg    <  1:730280/101‑1 (MQ=255)
                                                                                                   ccg.GGGCCCCAAGGCCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACg    <  2:6550759/101‑1 (MQ=255)
                                                                                                    cg.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACgg   >  2:4235198/1‑101 (MQ=255)
                                                                                                    cg.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGGTGCGCTTGATCGAAACgg   >  1:1958537/1‑101 (MQ=255)
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ACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACC.GCCG.GGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGC.CCA.TGGCGGGCGCTTCTC.GCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACGGT  >  NC_014034/1737022‑1737222

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG