Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 +C 2.1% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7851.→C 2.1% 13.2 ‑0.3 ‑0.5329intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (171/151):  total (174/155)
Fisher's exact test strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.19e-01

Only 100 of 342 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGAC  >  NC_014034/1740690‑1740882
                                                                                                  |                                                                                                 
 cGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAgg                                                                                              >  2:3605878/1‑101 (MQ=255)
 cGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAgg                                                                                              >  1:3991444/1‑101 (MQ=255)
    gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGa                                                                                           >  2:5214799/1‑101 (MQ=255)
     gtCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGAt                                                                                          <  1:7100173/100‑1 (MQ=255)
      tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATg                                                                                         >  1:412880/1‑101 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGc                                                                                        <  1:6341906/101‑1 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGc                                                                                        <  1:235909/101‑1 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGc                                                                                        >  1:3553053/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGcc                                                                                       >  1:1152790/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGcc                                                                                       >  1:3798921/1‑101 (MQ=255)
         aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCt                                                                                      <  1:5644367/101‑1 (MQ=255)
          gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCCCTGCCCCCGC.CGAGGCGATGCCTc                                                                                     <  2:2568959/101‑1 (MQ=255)
             gACGGAAGAACTGCTTCTGGACTATCTCGAAAGCAAATACGCCGGGATCTACCGGCTTTGAGGGC.GAGGGGGCGCGGCCCCCGG.CGAGGCGATGCCTCGGc                                                                                  <  2:5438496/101‑1 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                  >  2:5622123/1‑101 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                  >  1:7136552/1‑101 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCCCGGc                                                                                  >  2:1494581/1‑101 (MQ=255)
              aCGGAAGAACCGCTTCTGGACCATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                                 <  2:6398227/101‑1 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                               >  1:1064164/1‑101 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                               >  2:1951975/1‑101 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                               <  1:4265184/101‑1 (MQ=255)
                 gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGccccc                                                                              >  1:6258935/1‑101 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                             >  1:2577552/1‑101 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                             <  1:6647599/101‑1 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                            >  2:6240787/1‑101 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                           <  2:680104/101‑1 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                          >  2:1200429/1‑101 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                         >  2:712773/1‑101 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                         >  1:3070104/1‑101 (MQ=255)
                       ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                        <  1:4633864/101‑1 (MQ=255)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                      <  2:1645560/101‑1 (MQ=38)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                      <  2:1333741/101‑1 (MQ=38)
                           ttggggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGGTAttt                                                                    <  2:3516000/97‑1 (MQ=255)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                    >  2:1063673/1‑101 (MQ=38)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                    >  1:6455786/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                   >  1:2814790/1‑101 (MQ=38)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                             <  1:4382467/101‑1 (MQ=38)
                                   tATCTGGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.TAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                            <  2:1912627/101‑1 (MQ=37)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                            <  1:294006/101‑1 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                            <  2:4302467/101‑1 (MQ=38)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                        <  1:5499331/101‑1 (MQ=37)
                                       ccGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                        <  2:5974609/100‑1 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                       <  1:1952047/101‑1 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                       >  2:6823305/1‑101 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                       >  1:5747516/1‑101 (MQ=37)
                                         gAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                      <  2:3946780/101‑1 (MQ=37)
                                          aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                     >  2:2958889/1‑101 (MQ=37)
                                          aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                     >  1:2046554/1‑101 (MQ=37)
                                          aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                     >  1:5459807/1‑101 (MQ=37)
                                            aGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                   >  1:4903131/1‑101 (MQ=37)
                                             gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                  <  2:5922371/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCCAGATGAAAGGCac                                                 >  2:2355527/1‑100 (MQ=37)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                                 >  2:1015217/1‑101 (MQ=37)
                                               aaaTCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                                <  1:3456864/101‑1 (MQ=37)
                                               aaaTACGCCGCGCTCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                                >  1:5174635/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                                >  1:2997105/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                                >  1:2169657/1‑101 (MQ=37)
                                                   ccgccgcGATCTCCCGGCTTTGGGGGCGGG.GGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                                            <  1:2472304/100‑1 (MQ=25)
                                                   aCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                                            >  1:4298896/1‑101 (MQ=37)
                                                   aCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCCGGATACTTCGGGCAAGGAGaaaaggaacgggg                                            >  1:3266011/1‑91 (MQ=255)
                                                    cgccgcGTTCTCCCGGCTTTGAGGGC.GAGGGGGCTCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGtt                                           <  1:3925885/101‑1 (MQ=25)
                                                    cgccgcGATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGtt                                           <  1:407781/101‑1 (MQ=38)
                                                       cgcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCa                                        <  1:6787144/101‑1 (MQ=37)
                                                       cgcgATCGACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCa                                        <  2:5086048/101‑1 (MQ=37)
                                                        gcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                       >  1:452359/1‑101 (MQ=37)
                                                        gcgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                       >  2:1582695/1‑101 (MQ=37)
                                                         cgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                                      <  1:1448338/101‑1 (MQ=37)
                                                         cgATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                                      >  2:1440340/1‑101 (MQ=37)
                                                          gATCTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                     >  1:80734/1‑101 (MQ=37)
                                                             cTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                  <  2:2483771/101‑1 (MQ=25)
                                                             cTACCGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                  >  2:3006070/1‑101 (MQ=25)
                                                               cccGGCTTGGGGGCC.GAGGGGGCGCCCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                                <  2:2150701/100‑1 (MQ=18)
                                                                ccGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGGTGAAAGGCATCCGGTTTCATCGTGCTTc                               >  2:6886102/1‑101 (MQ=25)
                                                                 gcgCTTTGGGGGC.GG.GGGGGCCCGCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                              <  2:6935103/99‑1 (MQ=21)
                                                                 cGGCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                              <  1:5986547/101‑1 (MQ=25)
                                                                  ggCTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                             >  1:5267819/1‑101 (MQ=25)
                                                                  ggCTTGGGGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                             <  1:694358/101‑1 (MQ=21)
                                                                    cTTTGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                           >  2:773750/1‑101 (MQ=25)
                                                                     tttGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                          >  2:2906446/1‑101 (MQ=25)
                                                                      ttGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                         <  1:1808467/101‑1 (MQ=25)
                                                                      ttGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                         <  2:5747214/101‑1 (MQ=25)
                                                                       tGAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                        <  2:5853230/101‑1 (MQ=25)
                                                                        gAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                       >  2:4607596/1‑101 (MQ=25)
                                                                        gAGGGC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                       >  1:5940830/1‑101 (MQ=25)
                                                                          ggggggggggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                      <  1:6095381/93‑1 (MQ=21)
                                                                          ggggcgggggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                      <  2:5311026/93‑1 (MQ=21)
                                                                           ggC.GAGGGGGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg                    >  1:4832887/1‑101 (MQ=21)
                                                                              cgagggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggg                  >  2:6449074/5‑101 (MQ=255)
                                                                               gagggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg                 >  2:6174720/4‑101 (MQ=255)
                                                                               gagggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg                 >  2:3154954/4‑101 (MQ=255)
                                                                               gaggcggCGCTGCCCTCTC.CCAGGCGATGCGTCGCCCCCCCCGGCTATGTCGGGCAAGATGAAGCGCATCCTGGTTCCTCTTGCTTCAAATACCCCggggt                 >  2:3319532/6‑100 (MQ=255)
                                                                                 gggggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa               >  1:6737664/2‑101 (MQ=255)
                                                                                 gggggCCCTGCCCCCGC.CGAGGCGATGCCTTGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa               <  1:502058/100‑1 (MQ=21)
                                                                                   gggCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt             <  2:2814688/101‑1 (MQ=255)
                                                                                    ggCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTc            <  2:2197704/101‑1 (MQ=255)
                                                                                       gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg         >  1:1020827/1‑101 (MQ=255)
                                                                                        cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc        >  1:766654/1‑101 (MQ=255)
                                                                                          gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg      >  2:6747909/1‑101 (MQ=255)
                                                                                             ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg    >  2:2687101/1‑101 (MQ=255)
                                                                                             ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg    >  1:2722755/1‑101 (MQ=255)
                                                                                              cgtg.cgAGGCGATGCCTCGGCACCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGAc  <  2:4882635/98‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGGCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGAC  >  NC_014034/1740690‑1740882

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG