BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,748,575	+G	100%	intergenic (‑279/‑119)	RCAP_rcc01616 ← / → gppA	hypothetical protein/guanosine‑5'‑triphosphate,3'‑diphosphate pyrophosphatase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,748,575	1	.→G	100.0%	43.8	196	intergenic (‑279/‑119)	RCAP_rcc01616/gppA	hypothetical protein/guanosine‑5'‑triphosphate,3'‑diphosphate pyrophosphatase
Reads supporting (aligned to +/- strand): new base (159/21): ref base (0/16): total (159/37)
Fisher's exact test strand distribution p-value = 1.07e-13
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.67e-01

Only 100 of 253 total aligned reads displayed.


CTTGCCCTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAGGGGGT  >  NC_014034/1748475‑1748670


cTTGCCCTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGc.c.c                                                                                                        >  1:5669575/1‑101 (MQ=255)


  tGCCCTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gg                                                                                                    >  2:2167140/1‑101 (MQ=255)


   gCCCTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..ggg                                                                                                   >  2:4706851/1‑101 (MQ=255)


    cccTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gggg                                                                                                  <  2:1565300/101‑1 (MQ=255)


    cccTGCGCGGCGGAGCCTCCGGCGGAGGGATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGGGGCCGCAGGC.C.C..tggg                                                                                                  >  2:6660897/1‑101 (MQ=255)


      cTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gggg.gg                                                                                               <  2:3219417/101‑2 (MQ=255)


       tGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gggg.ggc                                                                                              <  1:1394352/101‑3 (MQ=255)


        gcgcGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gggg.ggca                                                                                             <  2:1264198/101‑4 (MQ=255)


         cgcgGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..gggg.ggcag                                                                                            <  1:1978120/101‑5 (MQ=255)


           cggcggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAg.cg                                                                                         <  2:3140538/101‑1 (MQ=255)


            ggcggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAg.cg..c                                                                                      >  2:4993864/1‑101 (MQ=255)


              cggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..ccc                                                                                    <  2:1508793/101‑1 (MQ=255)


              cggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..ccc                                                                                    <  1:1797374/101‑1 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CCG.CG..cccc                                                                                   >  2:874712/1‑101 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..cccc                                                                                   <  2:5459551/101‑1 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..cccc                                                                                   >  2:1772441/1‑101 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..cccc                                                                                   <  2:5601506/101‑1 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCC.GGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..ccccc                                                                                  <  2:4141289/101‑1 (MQ=255)


               ggAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGCGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..cccc                                                                                   >  1:3331819/1‑101 (MQ=255)


                gAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..ccccc                                                                                  >  1:3960451/1‑101 (MQ=255)


                  gCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCg                                                                                >  1:6268181/1‑101 (MQ=255)


                        ggcggAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGc                                                                          >  2:4366083/1‑101 (MQ=255)


                         gcggAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCt                                                                         >  1:2049094/1‑101 (MQ=255)


                          cggAGGTTTTTGGGGCAAGATGAACCGGCCCCCCTCCTTCTTCCCTAAAATACCCGGGGGGGGGGCCGCGGCC.CGG..GGGG.G.CAG.CC..CCCCCCGCCTTGCtt                                                                        <  2:2249765/101‑1 (MQ=255)


                          cggAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCtt                                                                        >  2:4564192/1‑101 (MQ=255)


                           ggAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTc                                                                       >  1:30798/1‑101 (MQ=255)


                           ggAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTGGCTTc                                                                       >  1:996106/1‑101 (MQ=255)


                            gAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCg                                                                      >  2:5492009/1‑101 (MQ=255)


                            gAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCg                                                                      >  1:5245756/1‑101 (MQ=255)


                              ggTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGc                                                                    >  2:4907674/1‑101 (MQ=255)


                               gTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGcc                                                                   >  2:5350433/1‑101 (MQ=255)


                               gTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGcc                                                                   >  2:388660/1‑101 (MQ=255)


                                tATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCt                                                                  >  2:711156/1‑101 (MQ=255)


                                 aTTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCtt                                                                 >  2:5936163/1‑101 (MQ=255)


                                 aTTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCtt                                                                 >  1:6751988/1‑101 (MQ=255)


                                  tttAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTg                                                                >  2:6197532/1‑101 (MQ=255)


                                  ttgaGGGCAAGATGAAGCGGTCCCCCTTCTTCTTCCCTAAAATACCCGGGGGGGGGGCCGCGGCC.CGG..GGGG.G.CAG.CC..CCCCCCGCCTTGCTTCGGCCTTg                                                                <  2:2565179/98‑1 (MQ=255)


                                    tAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCg                                                              >  1:305499/1‑101 (MQ=255)


                                    tAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCg                                                              >  1:5756075/1‑101 (MQ=255)


                                    tAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCg                                                              >  2:3834364/1‑101 (MQ=255)


                                    tAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCg                                                              >  1:7107812/1‑101 (MQ=255)


                                      gggCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGAc                                                            >  1:3177723/1‑101 (MQ=255)


                                       ggCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACg                                                           >  2:1239487/1‑101 (MQ=255)


                                        gCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGt                                                          >  1:1256866/1‑101 (MQ=255)


                                         cAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTc                                                         >  1:5374882/1‑101 (MQ=255)


                                          aaGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCa                                                        >  2:1288502/1‑101 (MQ=255)


                                          aaGAGGAACCGGCCCCCCTTCATCTTCCCAAAAATCCCCGGGGGGGGGCCCCCGGGC.CGG..GGGG.G.CCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCa                                                        <  1:5121817/101‑1 (MQ=255)


                                          aaGACGAAGCGGAGCCCCTCCATCTTCCCACAAATCTCCCGGGGGGGGGCCCGAGGC.CGG..GGGG.G.CCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCa                                                        <  2:6826222/101‑1 (MQ=255)


                                            gATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACg                                                      >  2:6475389/1‑101 (MQ=255)


                                            gATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACg                                                      >  2:1160208/1‑101 (MQ=255)


                                             aTGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGa                                                     >  2:4782376/1‑101 (MQ=255)


                                              tGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGaa                                                    >  1:6117948/1‑101 (MQ=255)


                                              tGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGaa                                                    >  1:2692234/1‑101 (MQ=255)


                                               gAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAAc                                                   >  1:4048993/1‑101 (MQ=255)


                                                aaccGGTCCCCCTTCCTCTGTCCTCAAATCCCCGGGGGGGGGCCCGCAGGC.CGG..GGGG.G.CAG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGCACt                                                  <  1:5596561/98‑1 (MQ=255)


                                                 aaCGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACtt                                                 >  2:5690386/1‑101 (MQ=255)


                                                  aCGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTg                                                >  1:5789632/1‑101 (MQ=255)


                                                   cGGTGCCCCTTCATCTTGCCTCAAATATCCCGTGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGt                                               >  1:430824/1‑101 (MQ=255)


                                                    gggcccccTGCTTCTTCCCTAAAATCCCCGGGGGGGGGGCCGCCGGCGG.G..GGGG.G.CGG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTc                                              <  1:4483655/97‑1 (MQ=255)


                                                    ggTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTc                                              >  1:1332267/1‑101 (MQ=255)


                                                      tGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCAt                                            <  2:2009757/101‑1 (MQ=255)


                                                       cccccTTCCTCTTGCCAAAAATCCCCGGGGGGGGGGCCGCGGGC.CGG..GGGG.G.CGC.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATc                                           <  1:6523043/100‑1 (MQ=255)


                                                        ccctttctccTTGCCACAATTCTCCGGGGGGGGGGCCGCGGGC.CGG..GGGG.G.CCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCg                                          <  2:655706/92‑1 (MQ=255)


                                                           cTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGgc                                       >  1:5941524/1‑101 (MQ=255)


                                                            ttCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCGTGCGACGTCACGAACTTGTCATCGGgcg                                      >  1:4969644/1‑101 (MQ=255)


                                                             tCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGgcgc                                     >  2:2107733/1‑101 (MQ=255)


                                                              cctCTTCCCTAAATTACCCGGGGGGGAGCCCGAGGGC.CGG..GGGG.G.CCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGGACTTGTCATCGGGCGCt                                    <  2:1972182/99‑1 (MQ=255)


                                                              cATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCt                                    >  2:2867325/1‑101 (MQ=255)


                                                                 cTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGa                                 >  1:1073744/1‑101 (MQ=255)


                                                                   tGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAAc                               >  2:3463587/1‑101 (MQ=255)


                                                                    gCCTCAAATACCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAAcc                              <  1:6938440/101‑1 (MQ=255)


                                                                    cccTCAAATCCCCGGGGGGGGGGCCGCAGCC.C.CG.GGGG.G.CAG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAAcc                              <  2:1103701/100‑1 (MQ=255)


                                                                     ccTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTGACGAACTTGTCATCGGGCGCTTGAACCt                             >  1:6187480/1‑101 (MQ=255)


                                                                      cTCAAATCCCCGGGGGGGAGGCCGCAGGC.CGG..GGGG.G.CCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCtt                            <  1:1706770/101‑1 (MQ=255)


                                                                      cTCAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCtt                            >  1:6398739/1‑101 (MQ=255)


                                                                      cTCAAATACCGGGGGGGGGGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCtt                            <  2:4330352/101‑1 (MQ=255)


                                                                       ccaaaatccccGGGGGGGGGCCCGCGGC.C.CGGGGGG.G.CCC.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCtt                            <  1:1071390/93‑1 (MQ=255)


                                                                        cAAATATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCg                          >  2:355890/1‑101 (MQ=255)


                                                                         aaaTATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCgg                         >  1:4600290/1‑101 (MQ=255)


                                                                          aaTATCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGc                        >  1:7936/1‑101 (MQ=255)


                                                                           ataccggggggggGGCCCGCGGGC.CGG..GGGG.G.CGG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGcc                       <  1:2835299/94‑1 (MQ=255)


                                                                             atCCCGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGcccc                     >  1:2739816/1‑101 (MQ=255)


                                                                              cccgggggggCGGCCGCGGCC.CGG..GGGG.G.CGC.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGccccc                    <  2:5226858/97‑1 (MQ=255)


                                                                               gcgggggggGGGCCGGCGGG.C.C..GGGG.G.CCC.CCCCCCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGccccc                    <  1:365424/98‑1 (MQ=255)


                                                                                cgggggggGGGCCGCGGGC.CGG..GGGG.G.CCC.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGccccccc                  <  2:6059287/99‑1 (MQ=255)


                                                                                ccGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGccccccc                  <  2:4048817/101‑1 (MQ=255)


                                                                                 gggggggGGGCCGGCGGC.CGG..GGGG.G.CCGCCC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGccccccc                  <  1:2241690/100‑1 (MQ=255)


                                                                                 cGGGGGGAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGACCCTCCGGCCCCCCcg                 >  2:3314625/1‑101 (MQ=39)


                                                                                  ggggggAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCcgc                >  1:6895017/1‑101 (MQ=255)


                                                                                  ggggggAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCCCCTGGCTTCGCCCTTGCACCGCCATAAATTTTTAACCGGCGGCTTG.ACCCTCGGcccccccccc               >  2:5273633/1‑98 (MQ=255)


                                                                                    ggggAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCgcgc              >  2:1841921/1‑101 (MQ=255)


                                                                                     gggAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCt             >  1:2555186/1‑101 (MQ=255)


                                                                                       gAGGCCGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTAc           >  2:3205172/1‑101 (MQ=255)


                                                                                       gAGCCCGCGGGC.C.GG.GGGG.G.AAG.CG..CCCGCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTAc           <  2:1211809/101‑1 (MQ=255)


                                                                                         gccggCAGCC.C.C..GGGGTG.CCC.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCt         <  2:358972/97‑1 (MQ=255)


                                                                                          gCCGCGGGC.C.G..GGGG.GCCCG.CC..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTa        <  2:2739714/101‑1 (MQ=255)


                                                                                            cGCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAgg      >  2:4590644/1‑101 (MQ=255)


                                                                                             gCAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAggg     >  1:2223658/1‑101 (MQ=255)


                                                                                              cAGGC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAgggg    >  1:2759963/1‑101 (MQ=255)


                                                                                                ggC.C.CG.GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAGGGGGt  >  1:156745/1‑101 (MQ=255)


CTTGCCCTGCGCGGCGGAGCCTCCGGCGGAGGTATTTAGGGCAAGATGAAACGGTGCCCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCGCAGGC.C.C..GGGG.G.CAG.CG..CCCCCCGCCTTGCTTCGGCCTTGCGACGTCACGAACTTGTCATCGGGCGCTTGAACCTTCGGCCCCCCCGCGCTACCTAGGGGGT  >  NC_014034/1748475‑1748670

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG