Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 94.4% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G94.4% 32.4 ‑2.0 ‑0.2232coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (82/136):  ref base (2/11):  total (84/148)
Fisher's exact test strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.86e-01

Only 100 of 256 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGGGCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462
                                                                                                       |                                                                                              
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.c                                                                                                 >  2:4631193/1‑101 (MQ=255)
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGGGGT.c                                                                                                 >  2:3200026/1‑101 (MQ=255)
 gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cg                                                                                                <  1:1309005/101‑2 (MQ=255)
 gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cg                                                                                                <  2:206400/101‑2 (MQ=255)
  ggCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cgc                                                                                               <  1:220711/100‑3 (MQ=255)
    cGGCAGCGTCGGCGGTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cgcgc                                                                                             <  1:6537786/101‑5 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cgcgcg                                                                                            >  1:5246669/1‑96 (MQ=255)
       cAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.cgcgcgcg                                                                                          >  2:705311/1‑94 (MQ=255)
            tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCtt                                                                                   <  1:5506997/101‑1 (MQ=255)
            tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCtt                                                                                   >  1:209334/1‑101 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCttt                                                                                  >  2:248394/1‑101 (MQ=255)
              ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTg                                                                                 >  1:5202888/1‑101 (MQ=255)
               gCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGc                                                                                <  1:5763240/101‑1 (MQ=255)
                 gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCt                                                                              <  1:1082564/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCtt                                                                             >  2:1186527/1‑101 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCtt                                                                             <  2:3955919/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGG.CGCG.CG.CGGGCTTTGCCtt                                                                             >  1:4958744/1‑101 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTg                                                                            <  1:642250/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCTCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCCTgg                                                                           <  1:6840089/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:6088117/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:4902777/1‑101 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        >  1:5642920/1‑101 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:3032734/101‑1 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCTGCTTTGGGCGCGGTCGATGT.CGGGCATGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:6324624/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGAGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCgg                                                                     >  2:3449135/1‑100 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:1669697/101‑1 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:892049/101‑1 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:5353239/101‑1 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:3303608/1‑101 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  1:2746196/101‑1 (MQ=255)
                               ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:110459/1‑101 (MQ=255)
                                gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               >  2:6357256/1‑101 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCCGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              >  2:2749513/1‑101 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  2:2983078/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:3586525/1‑101 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTGGATGTGG.GGCAGGGGGCGGGGGTGG.GG.CG.CGGGCGTTGCCTTGGGGTGGGGGCGCTTACgg                                                          >  2:2274655/1‑101 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         <  1:1959084/101‑1 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  1:2835330/101‑1 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  2:6735412/101‑1 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                      <  2:5660380/101‑1 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                      <  1:850069/101‑1 (MQ=255)
                                          gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  1:5802565/101‑1 (MQ=255)
                                          gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:3353634/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  2:6201619/1‑101 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  1:3097782/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  1:2609630/1‑101 (MQ=255)
                                              ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  2:4181719/101‑1 (MQ=255)
                                              ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 >  2:5430916/1‑101 (MQ=255)
                                               gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  2:4999282/101‑1 (MQ=255)
                                               gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGCTGGT.CGCG.CG.CGGGCTTTGCATTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4642168/101‑1 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               >  2:6440243/1‑101 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  1:2336519/101‑1 (MQ=255)
                                                   aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:4679169/1‑101 (MQ=255)
                                                   aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            <  1:1842029/101‑1 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  1:5944218/1‑101 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  2:506706/1‑101 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           <  1:4072920/101‑1 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  2:4864399/1‑101 (MQ=255)
                                                     aTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  2:3555544/101‑1 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  2:5537120/101‑1 (MQ=255)
                                                         tcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  1:379252/101‑1 (MQ=255)
                                                         tcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  1:2456487/101‑1 (MQ=255)
                                                         tcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  1:1755175/101‑1 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:1038665/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:3758704/1‑101 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:1427367/101‑1 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:6909414/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   >  2:5316025/1‑101 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  2:5818599/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:5394685/101‑1 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:1021364/1‑101 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  2:2180194/101‑1 (MQ=255)
                                                              gCTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 >  2:4296402/1‑101 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  1:1173201/101‑1 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  1:3318012/101‑1 (MQ=255)
                                                                tttGGGCGCGGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  1:4014242/1‑101 (MQ=255)
                                                                      cgcgGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGACTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         <  1:7071285/101‑1 (MQ=255)
                                                                       gcgGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  1:4161833/1‑101 (MQ=255)
                                                                        cgGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2368097/101‑1 (MQ=255)
                                                                        cgGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:604144/101‑1 (MQ=255)
                                                                        cgGTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       >  2:2513553/1‑101 (MQ=255)
                                                                         ggTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1766191/101‑1 (MQ=255)
                                                                         ggTCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:4416347/1‑101 (MQ=255)
                                                                           tCGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    <  2:1023745/101‑1 (MQ=255)
                                                                            cGATGT.CGGGCAGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  2:1583049/101‑1 (MQ=255)
                                                                                  tcgggcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:2444349/93‑1 (MQ=255)
                                                                                    gggcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:4583727/7‑101 (MQ=255)
                                                                                      gcagggggCGGTGGT.CTCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:5913675/97‑1 (MQ=255)
                                                                                      gcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          >  1:6706309/5‑101 (MQ=255)
                                                                                      gcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:4066790/97‑1 (MQ=255)
                                                                                       cagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  1:613519/98‑1 (MQ=255)
                                                                                       cagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  2:2095785/4‑101 (MQ=255)
                                                                                       cagggggCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  1:3392724/4‑101 (MQ=255)
                                                                                        agggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  2:6570033/99‑1 (MQ=255)
                                                                                           gggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     >  2:681727/1‑101 (MQ=255)
                                                                                           gggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:4416125/101‑1 (MQ=255)
                                                                                            ggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  1:628233/101‑1 (MQ=255)
                                                                                            ggCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    >  1:1350242/1‑101 (MQ=255)
                                                                                              cGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:4535160/101‑1 (MQ=255)
                                                                                              cGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:1795207/101‑1 (MQ=255)
                                                                                                       |                                                                                              
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGGGCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG