Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,823,740 +C 100% intergenic (+25/‑148) RCAP_rcc01687 → / → RCAP_rcc01688 HK97 family phage major capsid protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,823,7401.→C100.0% 94.0 268intergenic (+25/‑148)RCAP_rcc01687/RCAP_rcc01688HK97 family phage major capsid protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (156/80):  ref base (1/30):  total (158/110)
Fisher's exact test strand distribution p-value = 4.03e-12
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-03

Only 100 of 314 total aligned reads displayed.

CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835
                                                                                                      |                                                                                               
 tATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c                                                                                                >  1:124327/1‑101 (MQ=255)
 tATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.c                                                                                                >  1:728231/1‑101 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGTTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.cc                                                                                               <  1:6436581/101‑1 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.cc                                                                                               >  2:5965393/1‑101 (MQ=255)
   tGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.ccc                                                                                              <  1:2171047/101‑1 (MQ=255)
    gCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.cccc                                                                                             <  2:3098612/101‑2 (MQ=255)
    gCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.cccc                                                                                             <  1:5523764/101‑2 (MQ=255)
     cGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.ccccg                                                                                            <  1:5299804/101‑3 (MQ=255)
      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.ccccgg                                                                                           <  2:2544032/101‑4 (MQ=255)
      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.ccccgg                                                                                           <  2:3915644/101‑4 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTgc                                                                                        >  1:5153196/1‑101 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTgc                                                                                        >  1:2382844/1‑101 (MQ=255)
           aGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTgcc                                                                                       >  1:6106587/1‑101 (MQ=255)
            gCGGGTGGGCGGGGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTgccg                                                                                      <  1:1866706/101‑1 (MQ=255)
                 tGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGCTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGt                                                                                 >  2:5540858/1‑101 (MQ=255)
                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGtt                                                                                <  2:6717898/101‑1 (MQ=255)
                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTc                                                                               <  1:5246047/101‑1 (MQ=255)
                   ggcggcGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTc                                                                               <  2:6559606/101‑1 (MQ=255)
                    ggggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCg                                                                              <  2:2512042/99‑1 (MQ=255)
                    gcggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCg                                                                              >  2:1215788/1‑101 (MQ=255)
                        cgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCgg                                                                          <  1:6975921/101‑1 (MQ=255)
                         gATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGt                                                                         >  1:2543797/1‑101 (MQ=255)
                          aTGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTc                                                                        >  1:1180888/1‑101 (MQ=255)
                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTcg                                                                       <  1:5840094/101‑1 (MQ=255)
                           tGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTcg                                                                       >  1:5897008/1‑101 (MQ=255)
                            gTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTcgg                                                                      >  1:4545693/1‑101 (MQ=255)
                              cAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGGA.AGCCCGGCTGCCGCCGGGTCGCCGGGcgggg                                                                    >  2:2273949/1‑100 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  1:1010765/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  1:3399460/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCgggcgggc                                                                >  2:540123/1‑101 (MQ=255)
                                   aTTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGggcgggcg                                                               >  2:1474522/1‑101 (MQ=255)
                                    tttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGgcgggcgc                                                              <  2:4868748/101‑1 (MQ=255)
                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcggg                                                           >  1:445692/1‑101 (MQ=255)
                                        gccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcgggc                                                          >  1:373313/1‑101 (MQ=255)
                                        gccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcgggc                                                          >  1:3505735/1‑101 (MQ=255)
                                        gccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGgcgggcgcgggc                                                          <  2:6466322/101‑1 (MQ=255)
                                         cggcgTTCAAGTTGGTGAAGTTCGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCt                                                         <  1:1788867/99‑1 (MQ=255)
                                          cgcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCtt                                                        >  1:6210875/1‑101 (MQ=255)
                                           gcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTc                                                       >  2:4285151/1‑101 (MQ=255)
                                            cgATCAAGCTTTTGAAGTTTGCCGCCTCGTAACGGGGCGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                      <  1:3131548/101‑1 (MQ=255)
                                            cgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                      >  1:6742242/1‑101 (MQ=255)
                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgg                                                    <  2:233743/101‑1 (MQ=255)
                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgc                                                    >  2:3385678/1‑100 (MQ=255)
                                                   gCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCt                                               <  2:834317/101‑1 (MQ=255)
                                                    cTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTg                                              >  1:4213209/1‑101 (MQ=255)
                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGc                                             <  1:892020/101‑1 (MQ=255)
                                                     ggtggAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGc                                             <  1:2242026/97‑1 (MQ=255)
                                                      gTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGcc                                            >  1:6559412/1‑101 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                           >  1:3967949/1‑101 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          >  2:1922629/1‑101 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          >  2:6794735/1‑101 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          <  1:4953652/101‑1 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                          >  1:3461945/1‑101 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                      >  2:4155986/1‑101 (MQ=255)
                                                             tttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgc                                     >  1:7051194/1‑101 (MQ=255)
                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   <  1:2663365/101‑1 (MQ=255)
                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   >  1:3764250/1‑101 (MQ=255)
                                                               tGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctc                                   >  2:3820065/1‑101 (MQ=255)
                                                                gccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctcc                                  >  1:3856157/1‑101 (MQ=255)
                                                                gccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctcc                                  <  1:1659564/101‑1 (MQ=255)
                                                                 ccgccTCGTAGCGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccc                                 <  2:1444075/101‑1 (MQ=255)
                                                                 ccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccc                                 >  1:6308792/1‑101 (MQ=255)
                                                                 ccgccTCGGAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccc                                 <  2:2102081/101‑1 (MQ=255)
                                                                  cgccTCGTAACGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                                <  2:4955632/101‑1 (MQ=255)
                                                                  cgccCCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGCCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                                <  1:5107740/101‑1 (MQ=255)
                                                                   gccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctc                               <  2:372067/101‑1 (MQ=255)
                                                                    ccTCTTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              <  2:628383/101‑1 (MQ=255)
                                                                    ccTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccctcc                              >  2:6993580/1‑101 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTCGCTGCTCCCtccg                             >  1:4026136/1‑101 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                             <  1:2180151/101‑1 (MQ=255)
                                                                       cGTAACGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtc                           <  1:2993768/101‑1 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          <  2:4535296/101‑1 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          >  2:3531342/1‑101 (MQ=255)
                                                                        ctagcGGGCGGGGAAGGGCCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                          <  2:1985571/97‑1 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        <  2:5471229/101‑1 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                        >  1:2882952/1‑101 (MQ=255)
                                                                            cGGGCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGt                      <  2:2882843/101‑1 (MQ=255)
                                                                            cGGGCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGGCCGAgg                      >  2:3198424/1‑100 (MQ=255)
                                                                             gggGGGTGAGGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                     <  1:97903/101‑1 (MQ=255)
                                                                             gggCGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTg                     >  1:1571500/1‑101 (MQ=255)
                                                                               gCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGc                   <  1:3600850/101‑1 (MQ=255)
                                                                               gCGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGc                   <  2:5274373/101‑1 (MQ=255)
                                                                                cGGTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCg                  <  1:5690445/101‑1 (MQ=255)
                                                                                cGGTGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCg                  >  1:5985333/1‑101 (MQ=255)
                                                                                  gTGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGt                <  2:1799802/101‑1 (MQ=255)
                                                                                   tGAAGGGGCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg               <  2:1589964/101‑1 (MQ=255)
                                                                                   tGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg               >  1:5005546/1‑101 (MQ=255)
                                                                                   tGAAGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTg               >  2:1647898/1‑101 (MQ=255)
                                                                                     aaGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGGGCg             >  1:3250507/1‑101 (MQ=255)
                                                                                      aGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgg            >  1:4083672/1‑101 (MQ=255)
                                                                                      aGGGGCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCgg            >  2:1048413/1‑101 (MQ=255)
                                                                                       ggggCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCCGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggg           <  2:3449974/101‑1 (MQ=255)
                                                                                         ggCCGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggggg         >  1:6198230/1‑100 (MQ=255)
                                                                                          gCCGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTc        <  1:6888188/101‑1 (MQ=255)
                                                                                           ccGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt       <  1:815214/101‑1 (MQ=255)
                                                                                           ccGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt       <  2:7134746/101‑1 (MQ=255)
                                                                                            cGGGGGCACC.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg      <  1:6819950/101‑1 (MQ=255)
                                                                                            cGGGGGCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg      >  1:1460018/1‑101 (MQ=255)
                                                                                             gggggCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGGGCGGGGTCTgc     >  1:4782085/1‑101 (MQ=255)
                                                                                               gggCA.ACCCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTgcgc   >  2:5864209/1‑101 (MQ=255)
                                                                                                      |                                                                                               
CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGCA.A.CCCGGCTGCCGCCGGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCGCC  >  NC_014034/1823640‑1823835

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG