Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,369 +C 100% intergenic (+31/+117) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,3691.→C100.0% 536.1 168intergenic (+31/+117)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (30/138):  ref base (0/0):  total (30/138)

Only 100 of 173 total aligned reads displayed.

GCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.G.GGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGG  >  NC_014034/1843276‑1843460
                                                                                                |                                                                                             
  gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                                       <  2:4261965/101‑1 (MQ=255)
   cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTc                                                                                      <  2:5146594/101‑1 (MQ=255)
     cGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCAc                                                                                    <  1:3940300/101‑1 (MQ=255)
      gAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCAcc                                                                                   <  1:4204954/101‑1 (MQ=255)
       aGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCAccc                                                                                  <  2:4623998/101‑1 (MQ=255)
        gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCAcccc                                                                                 <  1:5469264/101‑1 (MQ=255)
           gAACGGCCTTGTCGAAGCCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                                              <  2:6525908/101‑1 (MQ=255)
           gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                                              <  2:2571034/101‑1 (MQ=255)
           gAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCg                                                                              <  1:6521692/101‑1 (MQ=255)
             aCGGGCTTGTCGAAGACCCCGAATACATCGGCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCggg                                                                            <  2:1018011/101‑1 (MQ=37)
                gCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGata                                                                         <  1:2006060/101‑1 (MQ=38)
                gCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGata                                                                         <  1:6141740/101‑1 (MQ=38)
                 ccTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGatat                                                                        <  2:1331318/101‑1 (MQ=38)
                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                                     <  2:5908834/101‑1 (MQ=37)
                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                                     <  2:5433353/101‑1 (MQ=37)
                    tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                                     <  1:1237748/101‑1 (MQ=37)
                     gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                                    <  1:437346/101‑1 (MQ=37)
                     gTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTc                                                                    <  1:2477935/101‑1 (MQ=37)
                       cGAAGACCCCGATTACATCGTCAAGGCCACGAGCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGc                                                                  <  1:3542899/101‑1 (MQ=25)
                        gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGcc                                                                 <  2:2327595/101‑1 (MQ=37)
                        gAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGcc                                                                 <  1:5252293/101‑1 (MQ=37)
                         aaGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCa                                                                >  2:3220400/1‑101 (MQ=37)
                          aGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCaa                                                               <  2:6967424/101‑1 (MQ=37)
                           gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                                              <  2:3886733/101‑1 (MQ=37)
                           gACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                                              <  2:6379330/101‑1 (MQ=37)
                             ccccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGt                                                            <  2:2326367/101‑1 (MQ=37)
                             ccccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGt                                                            <  1:4585916/101‑1 (MQ=37)
                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                           <  1:7134057/101‑1 (MQ=37)
                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                           <  1:1743227/101‑1 (MQ=37)
                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                           <  1:103594/101‑1 (MQ=37)
                              cccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTg                                                           <  1:1854045/101‑1 (MQ=37)
                               ccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGa                                                          <  2:4067762/101‑1 (MQ=25)
                               ccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGa                                                          <  2:571276/101‑1 (MQ=25)
                                cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                         <  2:4326248/101‑1 (MQ=25)
                                cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                         <  1:2696716/101‑1 (MQ=25)
                                 gATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaaa                                                        <  2:1442090/101‑1 (MQ=25)
                                  aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCTCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAt                                                       <  1:6349999/101‑1 (MQ=25)
                                  aTTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGGGAAAt                                                       >  1:5688595/1‑101 (MQ=25)
                                    tACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATgg                                                     <  2:374482/101‑1 (MQ=25)
                                     aCATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCTCGCGATATTTTCGCCAAGGTGAAATGGa                                                    >  2:1559120/1‑101 (MQ=37)
                                      cATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                                   <  1:5452971/101‑1 (MQ=25)
                                      cATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaa                                                   <  2:1442046/101‑1 (MQ=25)
                                       aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                                  <  1:6758901/101‑1 (MQ=25)
                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                                 <  1:700108/101‑1 (MQ=25)
                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                                 <  2:2541877/101‑1 (MQ=25)
                                        tCGTCAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                                 <  2:3081318/101‑1 (MQ=25)
                                          gTCAAGGCCACGATCGAGTGACACGGGCGCA.GAGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAgg                                               <  2:5248648/101‑1 (MQ=37)
                                            cAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                                             <  1:1084019/101‑1 (MQ=25)
                                            cAAGGCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                                             <  1:583123/101‑1 (MQ=25)
                                             aaGGCCACGATCGAGTGACACGGGCGCAGG.GGGGGTCTGCCCCCCCCCCC.TGCGCATTCACCCCCCCCGCCATTTTCTCCCCGGGGAAAAGGAAGAggggg                                            >  2:6779181/1‑99 (MQ=255)
                                               ggCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                          <  1:3135314/101‑3 (MQ=25)
                                                gCCACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctc                                         <  2:6156286/101‑4 (MQ=25)
                                                 ccACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                        <  1:1643300/101‑5 (MQ=25)
                                                 ccACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                        <  2:5726708/101‑5 (MQ=25)
                                                 ccACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                        <  1:2089543/101‑5 (MQ=25)
                                                  cACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                                       <  1:6629021/101‑6 (MQ=25)
                                                  cACGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                                       <  2:6009499/101‑6 (MQ=25)
                                                  cACGATCGAGTGACACGGGCCCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgcaccg                                       <  1:5617727/101‑6 (MQ=21)
                                                    cGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                     <  1:3029757/101‑1 (MQ=25)
                                                    cGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                     <  1:5447806/101‑1 (MQ=25)
                                                    cGATCGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                     <  2:3201131/101‑1 (MQ=25)
                                                     gagcGAGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.g                                   <  2:6362063/98‑1 (MQ=21)
                                                          aGTGACACGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                              <  2:5287485/101‑3 (MQ=21)
                                                               cacGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCt                          <  2:2149331/101‑1 (MQ=21)
                                                               cacGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCt                          <  2:3174256/101‑1 (MQ=21)
                                                                acGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                         <  2:757064/101‑1 (MQ=21)
                                                                acGGGCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCtt                         <  1:4876964/101‑1 (MQ=21)
                                                                  gggCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                       <  2:373887/101‑1 (MQ=21)
                                                                  gggCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                       <  1:2985399/101‑1 (MQ=21)
                                                                  gggCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                       <  1:929904/101‑1 (MQ=21)
                                                                  gggCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc                       <  1:6893035/101‑1 (MQ=21)
                                                                   ggCGCAGG.GGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCa                      <  1:1586057/101‑1 (MQ=21)
                                                                    ggcacagcgggcCTCTG.CCCCCCTCTGCTTCGCATTCACCCCCCTGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCa                      <  1:3294720/93‑1 (MQ=19)
                                                                     cgcagg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATc                    <  1:1711077/96‑1 (MQ=21)
                                                                      gcagg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCt                   <  1:3491802/97‑1 (MQ=21)
                                                                       cagg.ggtgCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt                  <  1:112860/98‑1 (MQ=21)
                                                                       cagg.ggggCTCTG.CCCCCCTCTGCTGCGTATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt                  <  1:4971157/98‑1 (MQ=21)
                                                                       cagg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt                  <  1:1397215/98‑1 (MQ=21)
                                                                       cagg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt                  <  1:2083366/98‑1 (MQ=21)
                                                                        agg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTg                 <  1:6988301/99‑1 (MQ=21)
                                                                         gg.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc                >  1:3971653/2‑101 (MQ=21)
                                                                          g.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc               >  2:4886391/1‑101 (MQ=21)
                                                                          g.ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGcc               >  2:4384627/1‑101 (MQ=21)
                                                                            ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca              <  1:6909500/101‑1 (MQ=25)
                                                                            ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca              >  2:6052641/1‑101 (MQ=25)
                                                                            ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca              >  1:279651/1‑101 (MQ=25)
                                                                             gggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac             >  2:3274840/1‑101 (MQ=25)
                                                                             gggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac             <  2:1804542/101‑1 (MQ=25)
                                                                               gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa           <  1:777667/101‑1 (MQ=25)
                                                                               gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa           >  1:4549645/1‑101 (MQ=25)
                                                                                ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa          >  2:6874696/1‑101 (MQ=25)
                                                                                  tctgccccccTCTGCTGCGCATTCACCCTCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAat         <  1:5970356/97‑1 (MQ=37)
                                                                                    tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatat       <  2:5322042/99‑1 (MQ=25)
                                                                                    tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAatat       >  1:5049345/3‑101 (MQ=25)
                                                                                      ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATcc     >  2:5055256/1‑101 (MQ=37)
                                                                                      ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATcc     <  2:2790387/101‑1 (MQ=37)
                                                                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATccc    >  2:6376845/1‑101 (MQ=37)
                                                                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATccc    <  1:6119968/101‑1 (MQ=37)
                                                                                         cccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCgg  <  2:7043267/101‑1 (MQ=37)
                                                                                         cccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACAAATATCCCgg  >  1:4854253/1‑101 (MQ=38)
                                                                                                |                                                                                             
GCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.G.GGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAATATCCCGG  >  NC_014034/1843276‑1843460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG