Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,867,831 +C 3.0% intergenic (‑233/+82) RCAP_rcc01729 ← / ← RCAP_rcc01730 pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,867,8311.→C 3.0% 4.9 ‑2.9 ‑0.4132intergenic (‑233/+82)RCAP_rcc01729/RCAP_rcc01730pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (68/60):  total (68/64)
Fisher's exact test strand distribution p-value = 5.26e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.17e-01

Only 100 of 274 total aligned reads displayed.

AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.G.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867731‑1867928
                                                                                                       |                                                                                                       
aGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.g                                                                                                          <  1:6038684/101‑1 (MQ=255)
aGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.g                                                                                                          >  2:29181/1‑101 (MQ=255)
  gAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGAGAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGC.AA.g.c.g                                                                                                      >  1:3703224/1‑101 (MQ=38)
    aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAACCCCCGCGGCGCGG.AA.G.c.g.cc                                                                                                   >  2:2741721/1‑101 (MQ=38)
     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGCGCGA.AA.G.c.g.ccc                                                                                                  >  2:5788926/1‑100 (MQ=255)
     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.c.g.ccc                                                                                                  <  1:2830668/101‑2 (MQ=255)
     aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGC.AA.G.c.g.ccc                                                                                                  >  1:2619550/1‑100 (MQ=255)
      aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCCGCGGCGCGG.AA.G.g.g.cccc                                                                                                 >  1:3279472/1‑99 (MQ=37)
      aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCCCCCCCCGCGGCGCGA.AA.A.c.g.cccc                                                                                                 >  1:194716/1‑99 (MQ=37)
         aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.c.g.cccgcca                                                                                              <  1:2662312/101‑6 (MQ=255)
          cggttttcCCCCGCCCGTGTCGCGACAGCGTTCCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.GCC.C.CCGCCA.t                                                                                             <  2:1704092/97‑1 (MQ=21)
              ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggc                                                                                         <  1:4731909/101‑1 (MQ=255)
               ttcccccGCCCGTGTCGCGACAGCGTGGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggcg                                                                                        <  2:5618132/98‑1 (MQ=21)
                ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCCGCCCCCGCGGCGCGA.AA.G.C.GCCCGCCC.Tgggcgg                                                                                       >  1:2263566/1‑101 (MQ=21)
                gcttccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCCCCA.Tcggcgg                                                                                       >  2:6036298/5‑101 (MQ=21)
                 tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggcggg                                                                                      <  1:39924/101‑1 (MQ=255)
                 cccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggcggg                                                                                      <  1:984438/100‑1 (MQ=255)
                  ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggcgggg                                                                                     <  2:5314008/101‑1 (MQ=255)
                   cagcccggtgtGGCTACAGCTTGGCAAAGCCCGCATCACCAAGCACGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.C.C.GCCCGCCA.Tgggcggggc                                                                                    <  2:5535593/94‑1 (MQ=14)
                    ccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.Tgggcggggcg                                                                                   <  2:6535867/101‑1 (MQ=255)
                      gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.CA.A.C.GCCCGCCC.CGGgcgggggggg                                                                                 >  1:6731333/1‑101 (MQ=18)
                      gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAACCCAGCCCCCGCGGCGCGACAA.G.G.C.CCGCCC.Cgcgcggggcgcg                                                                                 >  1:3108676/1‑99 (MQ=255)
                       cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGgggcgggc                                                                                <  1:723656/101‑1 (MQ=255)
                       cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGG.AA.G.C.GCCCGCCC.CGGgcggggggggg                                                                                >  2:2426452/1‑100 (MQ=18)
                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCt                                                                             <  2:5048669/101‑1 (MQ=255)
                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGA.AAGG.C.G.CCGCCC.CGGGCGGGGCGGgcgcg                                                                             >  2:815420/1‑100 (MQ=21)
                             tCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGGGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGc                                                                          <  1:5397011/101‑1 (MQ=17)
                               gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AA.G.C.GCCCGCCA.CGGGCGGGGGGGGGGCCGGccc                                                                        >  1:6485166/1‑100 (MQ=18)
                                cgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.Ga                                                                      <  2:568518/101‑1 (MQ=255)
                                  aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGTGGGGGGGGGGGCGGCC.GA.gg                                                                   >  1:4374788/1‑101 (MQ=18)
                                   cAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGG.AA.G.C.GCCCGCCC.TGGGCGGGGCGGGGGGCGGCC.GA.ggg                                                                  >  2:4116217/1‑101 (MQ=18)
                                   cAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AA.G.C.G.ACCCCACTGGGGGGGGCGGGGGCCgggccgggg                                                                    >  1:325525/1‑94 (MQ=18)
                                     gCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCACCCCCGCGGCGCGA.AA.A.C.GCCCCCCA.CGGGCGGGGCGGGGGCCGGGC.GA.GGG.cc                                                               >  1:4234085/1‑101 (MQ=21)
                                     gCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.cc                                                               <  2:3356467/101‑1 (MQ=255)
                                     gCGTTGAAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.cc                                                               <  2:3054145/101‑1 (MQ=255)
                                       gTTGCAAAGCCCGCAGCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCtt                                                             <  1:4080951/101‑1 (MQ=17)
                                       gTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCACCCCCGGCGGCGCGA.AA.G.C.GCCCGCCC.TGGGCGGGGCGGGGGCCTGCC.GG.ggggcct                                                              >  1:5949840/1‑97 (MQ=17)
                                        ttGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCttt                                                            <  1:1159958/101‑1 (MQ=17)
                                        ttGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCttt                                                            <  2:1218117/101‑1 (MQ=255)
                                         tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGAGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.AGGGCGGGGGGGGGGCGCGGC.GA.GGG.GCCTTg                                                           >  2:2029677/1‑101 (MQ=17)
                                           cAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTggg                                                         <  2:6291129/101‑1 (MQ=255)
                                            aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCC.TGGGCGGGGCGGGCGCCGGCC.GG.GGG.CCCTTgggg                                                        >  1:4340045/1‑101 (MQ=17)
                                             aaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGGGCCGGCC.CA.GGG.GCttttcggc                                                       >  1:2205004/1‑96 (MQ=17)
                                             aaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCCTTTGGGc                                                       >  2:4460298/1‑101 (MQ=17)
                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.AA.A.C.GCCCGCCA.TGGGCGGGGCGGGCGCCGGCC.GC.GGG.CCCTTTGGGcc                                                      >  1:4277810/1‑101 (MQ=14)
                                               gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCGGGCC.GA.GGG.GCCTTGGGGccc                                                     >  1:4222111/1‑101 (MQ=14)
                                               gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCCGGCCGGG.GGG.CCTTTGGGGcc                                                      >  1:5208073/1‑101 (MQ=17)
                                               gACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGccc                                                     <  2:6162743/101‑1 (MQ=255)
                                                gcggCATCCCCAAGGGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCGGGCC.GA.GGG.CCTTTGGGGccaa                                                    <  2:3624882/98‑3 (MQ=17)
                                                 ccGCACCCCCAAGCGCGCGGGAGGCCGGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGccccc                                                   <  1:4967204/101‑1 (MQ=14)
                                                  cccttCCCCAAGCGCGCGAGAGGCCGGCCCCCGCCCCGGGCGGCGCAA.AA.G.CTC.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGT.CCTTTGGGGCCCCcg                                                  <  2:5929870/97‑1 (MQ=14)
                                                   gCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.AA.G.A.GCCCGCCA.TGGGCGGGGGGGGCGCAGGCC.GA.GGG.GCGTTTGGGCCCCcgg                                                 >  1:1535352/1‑101 (MQ=17)
                                                     aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGGGGGCGCCGGCC.GG.GGG.GCTTTGGGGGCCCcggcg                                               >  1:1005811/1‑101 (MQ=17)
                                                       ccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGGCCCccgcggg                                             >  1:3762250/1‑100 (MQ=17)
                                                       ccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.CA.GGGCCCTTTGGGGCCCCcgcggg                                              >  2:5410705/1‑97 (MQ=17)
                                                        cccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCNNNNN.GG.GGG.CCTTTGGGGCCCCccgcgggc                                            >  1:3596075/1‑99 (MQ=17)
                                                          cAAGCGTGACAGAGGCCTGCGCCCAGCCCCCGCGGCGCGG.AA.G.CGG.CCGCCA.TGGGCGGGGCGGGGGGCGGGC.GA.GGG.GCGTTCGGGCGCCcgcgggcggg                                          >  2:6678897/1‑93 (MQ=9)
                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGGGCTGGCC.CA.GGGCCCTTTGGGGCCCCcgcgggccg                                           >  2:1835573/1‑94 (MQ=17)
                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAg                                          <  2:2238997/101‑1 (MQ=255)
                                                          cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.CA.GGG.CCTTTTGGGGCCCcggcgggccg                                          >  2:3486492/1‑97 (MQ=17)
                                                            aGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GAGGGG.CGTTTGGGGCCCCC.GCGGGCAggg                                        >  2:4309332/1‑101 (MQ=17)
                                                                cgcgGGAGCCCAGCGCCCGCCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGAt                                    <  2:1950508/101‑1 (MQ=17)
                                                                  ggagcgcccAGCGCCCCGCCCCGGCGGCGCGA.AG.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                  <  2:4038829/94‑1 (MQ=14)
                                                                  cgAGCGGCCAGCGCCCCGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                  <  2:325521/101‑1 (MQ=14)
                                                                  cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGGGCTGGCC.GA.GGG.GCCTTGGGGCCCCCCGCGGGCAGGGGGGTCt                                  >  1:2930090/1‑101 (MQ=14)
                                                                  cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGGGCTGGCC.GA.GGG.CCCTTGGGGCCCCCGGCGGGCCGGGGGGTCt                                  >  1:1434651/1‑101 (MQ=14)
                                                                  cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGGCCCCGGCGGGCAGGGGGGTCt                                  >  1:2900321/1‑101 (MQ=17)
                                                                   gagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                 <  2:2534772/101‑1 (MQ=255)
                                                                   gagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGGCCGGGGGGTCTg                                 >  1:1770961/1‑101 (MQ=17)
                                                                   gagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGGCCGGGGGGTCTg                                 >  1:6969616/1‑101 (MQ=17)
                                                                   gagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCCGCGGGCCGGGGGGTCTg                                 >  2:3027033/1‑101 (MQ=17)
                                                                   gagaGCCCAGCGCCCAGCCCCGGCGGCGCGA.AC.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                 <  2:3899133/101‑1 (MQ=17)
                                                                     gagGCCGGCGCCCAGCCCGGGGGGCGCGA.GG.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  1:5575579/101‑1 (MQ=17)
                                                                       ggCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                             <  2:2159258/101‑1 (MQ=255)
                                                                       gcccAGCGCCCGCCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                             <  1:4460520/99‑1 (MQ=17)
                                                                        gCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcg                            >  2:952450/1‑101 (MQ=255)
                                                                         ccggcgcCCAGCCCCGGCGGCGCGA.AA.GCC.C.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcgc                           <  2:5268826/98‑1 (MQ=18)
                                                                          cAGCGCCCAGCCCCGGCGGCGCGA.AA.GCC.C.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          <  2:4233890/101‑1 (MQ=255)
                                                                          cAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          <  1:211852/101‑1 (MQ=255)
                                                                          cAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGAGCa                          >  2:3204914/1‑101 (MQ=255)
                                                                           aGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                         >  2:2957958/1‑101 (MQ=255)
                                                                            gcgcCCCGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:2263471/101‑1 (MQ=255)
                                                                            gcgcCCAGCCCGGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:1559982/101‑1 (MQ=255)
                                                                             cccccAGCCCCGGGGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                       <  1:3843796/99‑1 (MQ=255)
                                                                              gcCCAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGc                      >  2:6010562/1‑101 (MQ=255)
                                                                               cccAGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  1:6208317/101‑1 (MQ=255)
                                                                                 cggCCCGGGCGGCGCAA.AC.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  1:1333877/99‑1 (MQ=18)
                                                                                 cacccccGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  2:6905436/98‑1 (MQ=255)
                                                                                  aGCCCCGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                  >  2:2006370/1‑101 (MQ=255)
                                                                                     cccGGCGGCGCGA.AC.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:2759339/101‑1 (MQ=255)
                                                                                     cccGGCGGCGCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGNTGCGCATGGCCGCGTcc               >  2:1078643/1‑100 (MQ=255)
                                                                                     cccGGCGGCGCGA.AA.C.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:6217868/101‑1 (MQ=255)
                                                                                       cggcggCGCGC.AG.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAGGGCCGCGTCAgc             <  1:6293777/101‑1 (MQ=18)
                                                                                         gcggcgCGA.AG.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcgc           <  2:6730912/101‑1 (MQ=255)
                                                                                         gcggcgCGA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGTGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGAATGGTTGCGGATGGCCGCGTTAgcgc           >  1:1044694/1‑101 (MQ=17)
                                                                                          cgccgcgA.AA.GCC.C.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          <  2:4660962/98‑1 (MQ=21)
                                                                                             cgcgA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCCGCGCcgcg       >  1:513453/1‑101 (MQ=255)
                                                                                             cgcgA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCcgcg       >  2:376642/1‑101 (MQ=255)
                                                                                                gA.AA.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccagatcg    >  1:4186477/1‑95 (MQ=21)
                                                                                                 a.aa.G.C.GCCCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa   >  1:6143559/1‑101 (MQ=255)
                                                                                                       |                                                                                                       
AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.AA.G.C.G.CCGCCA.TGGGCGGGGCGGGCGCTGGCC.GA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867731‑1867928

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG