BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,833	+C	94.8%	intergenic (‑235/+80)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,833	1	.→C	94.8%	20.7 ‑4.4 ‑0.4	173	intergenic (‑235/+80)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (68/96): ref base (0/9): total (68/105)
Fisher's exact test strand distribution p-value = 1.24e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.81e-01

Only 100 of 268 total aligned reads displayed.


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.G.CCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928


  aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.c.g.cc                                                                                                  <  2:2079230/101‑1 (MQ=255)


  aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAACCCCCGCGGCGC.GGAA.G.c.g.cc                                                                                                  >  2:2741721/1‑101 (MQ=38)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.c.g.ccc                                                                                                 <  2:4392935/101‑2 (MQ=255)


       aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.c.g.cccgcca                                                                                             <  1:2662312/101‑6 (MQ=255)


        cggttttcCCCCGCCCGTGTCGCGACAGCGTTCCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.GCC.C.CCGCCAt                                                                                             <  2:1704092/97‑1 (MQ=21)


            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGC.GCAA.A.C.g.ccccccctgggg                                                                                        >  1:4474341/1‑94 (MQ=25)


            ttttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATgggc                                                                                         <  1:4731909/101‑1 (MQ=255)


             tttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGGGGCCCGCCCCGGCCGCGC.CGAA.G.CGG.CCGCCAAtggcg                                                                                        >  2:2960301/1‑101 (MQ=21)


              ttCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCCCCCCCGCGGCCC.GAAA.G.C.c.ccccccccgggcgg                                                                                      >  1:6175297/1‑92 (MQ=37)


               tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCACGCCGGCGGCGC.GGAA.G.A.g.ccccccccggggggg                                                                                     >  2:5453215/1‑91 (MQ=25)


               tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATgggcggg                                                                                      <  1:39924/101‑1 (MQ=255)


               cccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATgggcggg                                                                                      <  1:984438/100‑1 (MQ=255)


                  ccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATgggcggggcg                                                                                   <  2:6535867/101‑1 (MQ=255)


                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGC.GACA.A.C.GCCCGCCCCGGgcgggggggg                                                                                 >  1:6731333/1‑101 (MQ=18)


                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGCGC.GAAA.G.C.GCCCGCCACGGggggggcggg                                                                                 >  1:2247455/1‑101 (MQ=21)


                    cgatctgtCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGgcggggcggg                                                                                 <  1:2801239/96‑1 (MQ=18)


                     cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGgggcgggc                                                                                <  1:723656/101‑1 (MQ=255)


                      ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGC.GCAA.G.C.GCCCGCCCCGGgcggggcggggg                                                                               >  1:4186841/1‑99 (MQ=21)


                        gtgtCGCGACAGCGTTGCAGAGACCGCATCCCAAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGGGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.Ct                                                                            <  2:5804379/101‑1 (MQ=18)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCGGCGGCGC.GAAAGG.C.G.CCGCCCCGGGCGGGGCGGgcg.cg                                                                            >  2:815420/1‑100 (MQ=21)


                         ggtCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTg                                                                           <  2:2009227/100‑1 (MQ=255)


                           tCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGGGAGAGGCCAGCCCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGc                                                                         <  1:5397011/101‑1 (MQ=17)


                               gACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGGGCCCAGGCCCGGCCGCGCGGAAC.G.G.G.CCGCCAAGGGGGGGGGGGGGG.CCGGGC.gcg                                                                    >  1:3280265/1‑99 (MQ=16)


                                aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CCGGCC.GGgg                                                                   >  1:4242445/1‑101 (MQ=18)


                                aCAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGTGGGGGGGGGG.GCGGCC.GAgg                                                                   >  1:4374788/1‑101 (MQ=18)


                                   gCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.cc                                                               <  2:5630000/101‑1 (MQ=255)


                                   gCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.cc                                                               <  2:3356467/101‑1 (MQ=255)


                                      ttGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCttt                                                            <  1:3257112/101‑1 (MQ=255)


                                      ttGCAAAGCCCGCACCCCCAGGCGCGCGAGGGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCttt                                                            <  1:2458928/101‑1 (MQ=17)


                                      ttGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCttt                                                            <  1:1159958/101‑1 (MQ=17)


                                      ttGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCttt                                                            <  2:1218117/101‑1 (MQ=255)


                                      tcccAAAGACCGCACCCCCAAGCGCGGGAGAGCCCAGCCCCCACCCCCGGCGGCGC.GAAA.GCC.C.CCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCttt                                                            <  1:4095527/98‑1 (MQ=14)


                                         cAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CGGGGC.GAGGG.CCCTTggg                                                         >  2:533092/1‑101 (MQ=17)


                                          aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCCTGGGCGGGGCGGGGG.CCGGCC.CCGGG.GCCTTgggg                                                        >  2:524673/1‑101 (MQ=17)


                                          aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCCTGGGCGGGGCGGGCG.CCGGCC.GGGGG.CCCTTgggg                                                        >  1:4340045/1‑101 (MQ=17)


                                          aaaGACCGCACCCACAAGCGGGGAGGAGGCCAGCGCCCAGCCCCGGGGGCGG.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTgggg                                                        <  2:4054571/101‑1 (MQ=14)


                                           aaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CCGGCC.CAGGG.GCttttcggc                                                       >  1:2205004/1‑96 (MQ=17)


                                             gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCCTGGGCGGGGCGGGGGCCGGGCC.ACGGG.CCTTTCGGGcc                                                      >  2:6014123/1‑101 (MQ=17)


                                             gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CGGGCC.GAGGG.GCCTTGGGGccc                                                     >  1:4222111/1‑101 (MQ=14)


                                             gACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CCGGCCGGGGGG.CCTTTGGGGcc                                                      >  1:5208073/1‑101 (MQ=17)


                                             gACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGccc                                                     <  2:6162743/101‑1 (MQ=255)


                                              cccGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCACCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGcccc                                                    <  1:6660422/100‑1 (MQ=17)


                                               ccGCACCCCCAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGccccc                                                   <  2:3029378/101‑1 (MQ=17)


                                               ccGCACCCCCAAGCGCGCGGGAGGCCGGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGccccc                                                   <  1:4967204/101‑1 (MQ=14)


                                                cccttCCCCAAGCGCGCGAGAGGCCGGCCCCCGCCCCGGGCGGCGC.AAAA.G.CTC.CCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGT.CCTTTGGGGCCCCcg                                                  <  2:5929870/97‑1 (MQ=14)


                                                cccacccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCcg                                                  <  1:371989/96‑1 (MQ=255)


                                                cccacccccAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCcg                                                  <  1:2866860/96‑1 (MQ=17)


                                                 gCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGC.GAAA.G.A.GCCCGCCATGGGCGGGGGGGGCG.CAGGCC.GAGGG.GCGTTTGGGCCCCcgg                                                 >  1:1535352/1‑101 (MQ=17)


                                                 gCACCCCCAGGCGCGCGAGGGCCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCcgg                                                 <  2:3279356/101‑1 (MQ=17)


                                                   aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGGGGGCG.CCGGCC.GGGGG.GCTTTGGGGGCCCcggcg                                               >  1:1005811/1‑101 (MQ=17)


                                                   aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCCTTGGGGCCCCccgcg                                               >  2:1981286/1‑101 (MQ=17)


                                                      cccagccgcgggAGAGCCCAGCCCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTc                                            <  2:3527152/95‑1 (MQ=14)


                                                      cccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CNNNNN.GGGGG.CCTTTGGGGCCCCccgcgggc                                            >  1:3596075/1‑99 (MQ=17)


                                                       ccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCa                                           <  1:5729092/101‑1 (MQ=255)


                                                        cAAGCGTGACAGAGGCCTGCGCCCAGCCCCCGCGGCGC.GGAA.G.CGG.CCGCCATGGGCGGGGCGGGGG.GCGGGC.GAGGG.GCGTTCGGGCGCCcgcgggcggg                                          >  2:6678897/1‑93 (MQ=9)


                                                        cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CTGGCC.CAGGGCCCTTTGGGGCCCCcgcgggccg                                           >  2:1835573/1‑94 (MQ=17)


                                                        cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAg                                          <  2:2238997/101‑1 (MQ=255)


                                                        cAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.A.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCCTTGGGGGCCCCCGCCGTCAg                                          >  2:3227728/1‑101 (MQ=17)


                                                         aggcgcgtGGGAGCCAAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAgg                                         <  2:3108563/99‑1 (MQ=14)


                                                         acgcgcgcGAGAGGCCAGCGCCCTGCGCGGGCGGCGC.GAAG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCGGGCGGTCAgg                                         <  2:4625931/99‑1 (MQ=14)


                                                         aaGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.GCTTTGGGGGCCCcggcgggccgg                                         >  1:3120229/1‑96 (MQ=17)


                                                              cgcgAGGGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGAt                                    <  2:3703075/101‑1 (MQ=255)


                                                                cgAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CTGGCC.GAGGG.GCCTTGGGGCCCCCCGCGGGCAGGGGGGTCt                                  >  1:2930090/1‑101 (MQ=14)


                                                                cgAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CTGGCC.GAGGG.CCCTTGGGGCCCCCGGCGGGCCGGGGGGTCt                                  >  1:1434651/1‑101 (MQ=14)


                                                                cgAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.A.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.CAGGG.CCTTTGGGGGCCCCCGCGGGCAGGGGGGTCt                                  >  2:2181149/1‑101 (MQ=17)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                 <  2:2534772/101‑1 (MQ=255)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGGCCGGGGGGTCTg                                 >  1:1770961/1‑101 (MQ=17)


                                                                 gagaGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGGCCGGGGGGTCTg                                 >  1:6969616/1‑101 (MQ=17)


                                                                 gagaGCCCAGCGCCCAGCCCCGGCGGCGC.GAAC.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                 <  2:3899133/101‑1 (MQ=17)


                                                                  agagGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGGG.CTGGCC.GAGGG.CCCTTGGGGGCCCCGGCGGGCCGGGGGGTCTGt                                >  2:2439247/1‑101 (MQ=14)


                                                                  agagGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.GCTTTGGGGGCCCCGGCGGTCCGGGGGGTccgg                                >  2:2366314/1‑98 (MQ=14)


                                                                  agacGCCACCCCCCCGCCCGGGCGGCGC.TATC.G.C.GCCCGCCGATGGCGGGGCGGGCG.CAGGCC.GAGGG.CCTTT.GGGCCCTCGGCGGGCAGGGGGaaatgct                               <  2:4736170/101‑7 (MQ=17)


                                                                   gagGCCGGCGCCCAGCCCGGGGGGCGC.GAGG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                               <  1:5575579/101‑1 (MQ=17)


                                                                   gagGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGGCCCCGGCGGGCAGGGGGGTCTGtt                               >  2:5579642/1‑101 (MQ=17)


                                                                    gggCCAGCGCCCAGCCCCGGCGGCGC.GAAG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                              <  2:6484836/100‑1 (MQ=255)


                                                                     gccccgcgcCCGGCCCGGGCGCGGC.GACC.G.C.GCCCGCCGTGGGCGGGGCGGGCG.CTGGCC.GAGGT.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                             <  2:5311268/96‑1 (MQ=17)


                                                                      gCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcg                            >  2:952450/1‑101 (MQ=255)


                                                                        cAGCGCCCAGCCCCGGCGGCGC.GAAA.GCC.C.CCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          <  2:4233890/101‑1 (MQ=255)


                                                                        cAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                          <  1:211852/101‑1 (MQ=255)


                                                                          gcgcCCAGCCCGGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATg                        <  2:1559982/101‑1 (MQ=255)


                                                                           cccccAGCCCCGGGGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                       <  1:3843796/99‑1 (MQ=255)


                                                                             cccAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  2:2929981/101‑1 (MQ=255)


                                                                             cccAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                     <  1:6208317/101‑1 (MQ=255)


                                                                              ccggCCCGGGCGGGGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcg                    <  2:5739254/98‑1 (MQ=18)


                                                                              ccAGCCCCGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcg                    >  2:2767299/1‑101 (MQ=255)


                                                                               cggCCCGGGCGGCGC.AAAC.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  1:1333877/99‑1 (MQ=18)


                                                                               cacccccGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                   <  2:6905436/98‑1 (MQ=255)


                                                                                   cccGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  1:4308856/101‑1 (MQ=255)


                                                                                   cccGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGNTGCGCATGGCCGCGTcc               >  2:1078643/1‑100 (MQ=255)


                                                                                   cccGGCGGCGC.GAAA.C.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa               <  2:6217868/101‑1 (MQ=255)


                                                                                    ccGGCGGCGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGGCAGGGGGATCTGTTGCGCATGGCCGCGTCAg              >  1:1887321/1‑101 (MQ=255)


                                                                                     cggcggCGC.GCAG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAGGGCCGCGTCAgc             <  1:6293777/101‑1 (MQ=18)


                                                                                      ggcggcGC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg            >  1:3787027/1‑101 (MQ=255)


                                                                                       gcggcgC.GAAG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcgc           <  2:6730912/101‑1 (MQ=255)


                                                                                       gcggcgC.GAAA.G.C.GCCCGCCATGGGCGGGGCGGGTG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGAATGGTTGCGGATGGCCGCGTTAgcgc           >  1:1044694/1‑101 (MQ=17)


                                                                                        cggcgC.GAAG.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          <  1:6054639/101‑1 (MQ=255)


                                                                                         cgatctgAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgcc          <  2:4186282/95‑1 (MQ=21)


                                                                                           cgc.gAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCCGCGCcgcg       >  1:513453/1‑101 (MQ=255)


                                                                                           cgc.gAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCcgcg       >  2:376642/1‑101 (MQ=255)


                                                                                               gAAA.G.C.GCCCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc    >  2:6066440/1‑101 (MQ=255)


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGC.GAAA.G.C.G.CCGCCATGGGCGGGGCGGGCG.CTGGCC.GAGGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCAA  >  NC_014034/1867733‑1867928

Base quality scores:


ATCG

< 3 ≤


ATCG

< 12 ≤


ATCG

< 20 ≤


ATCG

< 32 ≤


ATCG

< 41 ≤


ATCG