BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	89.1%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	89.1%	103.3 ‑0.8 ‑0.9	313	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (113/166): ref base (16/18): total (129/184)
Fisher's exact test strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.24e-01

Only 100 of 333 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.ACCCTCGGGGCTGCGG.T.A.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  1:7009051/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  1:2298711/1‑101 (MQ=255)


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcc                                                                                              >  1:1459776/1‑101 (MQ=255)


      ggcGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACAC.Ggccca                                                                                            <  2:1633686/99‑3 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggccca                                                                                            <  1:4455495/101‑3 (MQ=255)


       aCGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaa                                                                                           <  1:5221834/101‑4 (MQ=255)


         ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaacc                                                                                         <  1:1548856/101‑6 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccctc                                                                                      <  1:1018757/101‑9 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccctc                                                                                      <  1:5135463/101‑9 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Ggcccaaccctc                                                                                      <  2:5124810/101‑9 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTTAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGTGGGTCCACTCGGCCCAACCCTCgg                                                                                     >  2:4272579/1‑101 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCgg                                                                                     <  2:1997132/101‑1 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGAGCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGGGAACTCGGCCCAACCCTCgg                                                                                     >  2:2236201/1‑101 (MQ=255)


                ccGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCgggg                                                                                   >  1:5536046/1‑101 (MQ=255)


                ccGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCgggg                                                                                   >  1:1943051/1‑101 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCt                                                                                 >  1:6053769/1‑101 (MQ=255)


                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTg                                                                                <  2:4555715/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGc                                                                               <  2:4718637/101‑1 (MQ=255)


                     ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCg                                                                              <  2:3348388/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.t                                                                           <  2:6267170/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.t                                                                           >  2:803577/1‑101 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.t                                                                           >  2:6227324/1‑101 (MQ=255)


                        ttttCGGTCCAGCGCAGNGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.a                                                                         <  2:941926/101‑1 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.ag                                                                        <  1:278631/101‑2 (MQ=255)


                          ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.agc                                                                       <  1:4591741/101‑3 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.agcgc                                                                     <  2:6029048/101‑5 (MQ=255)


                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.agcgct                                                                    >  2:56002/1‑96 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTc                                                                   <  2:6430832/101‑1 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTc                                                                   <  2:2367207/101‑1 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCgg                                                                 <  1:6240631/101‑1 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCgg                                                                 <  1:3526471/101‑1 (MQ=255)


                                ccAGCCCAGGGGGAGGGTGATCGGGGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCgg                                                                 >  2:7027772/1‑101 (MQ=255)


                                 cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCATCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCggg                                                                <  1:3663971/101‑1 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGc                                                               >  2:5234988/1‑101 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGc                                                               <  2:3036088/101‑1 (MQ=255)


                                   gcgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCg                                                              >  2:1499148/1‑101 (MQ=255)


                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGt                                                             <  2:440607/101‑1 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGtt                                                            >  2:5951038/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.TCG.CGCTCGGGCGtttt                                                          <  1:5639518/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGtttt                                                          >  1:2114523/1‑101 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTc                                                         <  2:5785071/101‑1 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCg                                                        <  2:1171816/101‑1 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCg                                                        >  1:4036584/1‑101 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCgg                                                       <  2:2619671/101‑1 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATc                                                    >  1:1773203/1‑101 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCa                                                   <  2:6883029/101‑1 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCa                                                   <  2:3884823/101‑1 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCa                                                   <  2:121452/101‑1 (MQ=255)


                                                 tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGt                                                >  2:3823060/1‑101 (MQ=255)


                                                 tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGt                                                >  2:566306/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGtt                                               <  2:1458423/101‑1 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:6648538/1‑101 (MQ=255)


                                                      ggCGCGGCATGCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACTCCCGGGGCGGAGGAG.A.AGCTCGGGCGATTTCGGATCAAATTCCTc                                           >  2:3293189/1‑101 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:39136/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:580170/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGAGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  1:5951342/101‑1 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         >  1:2703878/1‑101 (MQ=255)


                                                         gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                        <  1:4108443/101‑1 (MQ=255)


                                                         gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                        <  2:2998277/101‑1 (MQ=255)


                                                          cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  1:2556598/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  1:791440/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  2:2443793/1‑101 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  1:676299/101‑1 (MQ=255)


                                                                ccTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                 >  1:1334998/1‑101 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                <  1:4895323/101‑1 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                <  1:222067/101‑1 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                <  1:6529456/101‑1 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:1886925/1‑101 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:1060168/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  2:3255401/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.TCG.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:4866432/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            >  2:2712147/1‑101 (MQ=255)


                                                                      ccGTGACGATCTTCTTGCTGCTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           <  1:4377347/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         >  1:2989163/1‑101 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACTCGGCCCAATCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        <  1:3372922/101‑1 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        >  1:4662091/1‑101 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        >  1:7105638/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:986446/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       >  2:3132060/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:4029831/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGCGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:5603351/101‑1 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  2:1344389/101‑1 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     >  2:3619833/1‑101 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  1:3195843/101‑1 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    <  2:6692815/101‑1 (MQ=255)


                                                                              aTCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   >  2:613936/1‑101 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  >  2:540388/1‑101 (MQ=255)


                                                                                cACCTTGCGGGGGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACCAGCAGGTCCTCGTCATCGGCGa                 >  2:3487777/1‑101 (MQ=255)


                                                                                 aCCTTGCGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.G.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                >  2:3461228/1‑101 (MQ=255)


                                                                                       cGGGTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc          >  1:3014319/1‑101 (MQ=255)


                                                                                        gggTGGTGAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         <  1:1808979/101‑1 (MQ=255)


                                                                                            ggtgAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  1:5030475/1‑101 (MQ=255)


                                                                                            ggtgAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:6626562/1‑101 (MQ=255)


                                                                                            ggtgAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:2873284/1‑101 (MQ=255)


                                                                                            ggtgAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     <  2:4661852/101‑1 (MQ=255)


                                                                                              tgAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg   <  1:6445449/101‑1 (MQ=255)


                                                                                               gAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  1:3823223/101‑1 (MQ=255)


                                                                                               gAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  >  1:4705288/1‑101 (MQ=255)


                                                                                               gAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  2:2666657/101‑1 (MQ=255)


                                                                                               gAACTCGGCCCAACCCTCGGGGCTGCGG.T.AGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc  >  1:1512012/1‑101 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.ACCCTCGGGGCTGCGG.T.A.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG