Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 1100.4 290coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (120/168):  ref base (0/2):  total (120/170)

Only 100 of 317 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCCCGG  >  NC_014034/1948775‑1948970
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ttttCGGTCCAGCGCAGNGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTa                                                                                                    <  2:941926/101‑1 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTag                                                                                                   <  2:6238714/101‑2 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTag                                                                                                   >  2:474491/1‑100 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTag                                                                                                   <  1:278631/101‑2 (MQ=255)
  ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTagc                                                                                                  <  2:3185758/101‑3 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTagcgct                                                                                               >  2:118440/1‑96 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                                              <  1:4698701/101‑1 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                                              <  2:6430832/101‑1 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                                              <  2:2367207/101‑1 (MQ=255)
        ccAGCCCAGGGGGAGGGTGATCGGGGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCgg                                                                                            >  2:7027772/1‑101 (MQ=255)
           gcgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCg                                                                                         >  2:1499148/1‑101 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                        <  2:440607/101‑1 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                       >  2:2146315/1‑101 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                       <  1:4071337/101‑1 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                       <  1:4325488/101‑1 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                     >  1:2114523/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                     <  2:264050/101‑1 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGgttt                                                                                     >  2:5013496/1‑101 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                    <  2:5785071/101‑1 (MQ=255)
                 ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTAGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttttgg                                                                                   >  1:3888518/1‑99 (MQ=255)
                  gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                                                  <  2:2619671/101‑1 (MQ=255)
                  gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                                                  <  1:1604498/101‑1 (MQ=255)
                    gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                                                >  1:5967814/1‑101 (MQ=255)
                    gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                                                >  1:1460313/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                               <  2:5977325/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                              <  2:3884823/101‑1 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                                           >  2:566306/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                          <  2:1458423/101‑1 (MQ=255)
                          gaTCGGCGCGGCATACTTGACCGTGACGATCACCTTGCGGGTGGTGACCTAG.GCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                          >  2:4660744/1‑101 (MQ=255)
                           aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTc                                                                         >  1:2280658/1‑101 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                       <  2:5309468/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                      >  2:5443858/1‑101 (MQ=255)
                               gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                                                     <  1:580170/101‑1 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                   <  2:1755752/101‑1 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                   <  2:2998277/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                                  <  1:1117828/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                                  <  1:2556598/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                                  <  1:4019637/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                                  >  1:4614508/1‑101 (MQ=255)
                                   ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                                                 >  1:660925/1‑101 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                                <  2:3591109/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACTAACa                                                              <  2:2705284/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                              >  1:3052861/1‑101 (MQ=255)
                                        ccTTGGCCCTGGCTATCACCTTGCGGGTTGTGAACT.CGGCCCAACCCTCGGGGCTGCGTTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAgg                                                            <  2:3289147/101‑1 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                           <  1:4895323/101‑1 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                          <  2:3351854/101‑1 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                          >  2:1886925/1‑101 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                          <  1:5235254/101‑1 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                         <  1:3008816/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                        >  1:7017901/1‑101 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                        >  1:4523767/1‑101 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                       >  1:1040622/1‑101 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                       <  1:3613189/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                       <  1:6215358/101‑1 (MQ=255)
                                              ccGTGCCGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTGCTCGACGAACAGGTCCTCg                                                      <  2:3797895/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                    >  1:2989163/1‑101 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                    <  1:5076952/101‑1 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                   >  1:4662091/1‑101 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                   >  1:7105638/1‑101 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                   <  1:803601/101‑1 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                                  >  1:5634892/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                                  <  1:56003/101‑1 (MQ=255)
                                                   aCGATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                                                 <  2:1031116/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                               <  2:6692815/101‑1 (MQ=255)
                                                      aTCACCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                                              >  2:613936/1‑101 (MQ=255)
                                                         aCCTTGCGGGTGGTGAANT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                           <  2:660905/101‑1 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                           >  2:3461228/1‑101 (MQ=255)
                                                          ccTTGCGGGTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg                                          <  1:2167060/101‑1 (MQ=255)
                                                                gggTGGTGAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                    <  1:1808979/101‑1 (MQ=255)
                                                                  gtggtgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt                                  <  2:468599/101‑1 (MQ=255)
                                                                   tggtgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg                                 <  1:6352478/101‑1 (MQ=255)
                                                                    ggtgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                                >  1:3756322/1‑101 (MQ=255)
                                                                    ggtgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                                >  2:2873284/1‑101 (MQ=255)
                                                                     gtgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGAt                               <  2:1334947/101‑1 (MQ=255)
                                                                      tgAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGCTg                              >  2:4189829/1‑101 (MQ=255)
                                                                       gAACT.CGGCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc                             >  1:1512012/1‑101 (MQ=255)
                                                                          actcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGccc                           <  1:6658355/91‑1 (MQ=255)
                                                                           ctcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGcccc                          <  2:5210073/92‑1 (MQ=255)
                                                                           ctcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGcccc                          <  1:551814/92‑1 (MQ=255)
                                                                            tcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                         <  1:315069/93‑1 (MQ=255)
                                                                            tcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                         >  2:2297933/9‑101 (MQ=255)
                                                                            tcggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                         <  2:3234021/93‑1 (MQ=255)
                                                                             cggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                        <  2:3958586/94‑1 (MQ=255)
                                                                              ggcccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGc                       >  1:1248202/7‑101 (MQ=255)
                                                                               ncnn.NCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCa                     <  1:6136239/100‑1 (MQ=255)
                                                                               gcccaaCCCGCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                      <  1:540400/96‑1 (MQ=255)
                                                                                cccaaCCCTCTGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCCGCGAAAGCGGCGGTGATGCCCAAGcta                     >  2:4596704/5‑99 (MQ=255)
                                                                                cccaaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCa                     <  2:1404255/97‑1 (MQ=255)
                                                                                   aaCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGc                  >  1:6780615/2‑101 (MQ=255)
                                                                                      ccTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTgg               <  2:6053457/101‑1 (MQ=255)
                                                                                      ccTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTgg               >  2:3666380/1‑101 (MQ=255)
                                                                                      ccTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTgg               >  2:6781458/1‑101 (MQ=255)
                                                                                        tCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTc             <  2:2538732/101‑1 (MQ=255)
                                                                                         cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg            >  2:6919931/1‑101 (MQ=255)
                                                                                         cGGGGCTGCGGTAGCGCTCGGGCGTTTTCGAATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg            >  2:1658067/1‑101 (MQ=255)
                                                                                           gggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt          >  1:2035520/1‑101 (MQ=255)
                                                                                             gCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc        <  1:3343558/101‑1 (MQ=255)
                                                                                             gCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc        <  1:3461352/101‑1 (MQ=255)
                                                                                              cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg       <  2:2820790/101‑1 (MQ=255)
                                                                                               tGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcgc      >  2:3078508/1‑101 (MQ=255)
                                                                                                      |                                                                                                 
TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCCCGG  >  NC_014034/1948775‑1948970

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG