Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,965,279 | +C | 89.5% | intergenic (+47/+45) | bdhA → / ← RCAP_rcc01818 | 3‑hydroxybutyrate dehydrogenase/hypothetical protein |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,965,279 | 1 | .→C | 89.5% | 13.1 ‑0.9 ‑0.8 | 57 | intergenic (+47/+45) | bdhA/RCAP_rcc01818 | 3‑hydroxybutyrate dehydrogenase/hypothetical protein |
Reads supporting (aligned to +/- strand): new base (30/21): ref base (5/1): total (35/22) | ||||||||||
Fisher's exact test strand distribution p-value = 3.89e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.76e-01 |
AGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCGGC > NC_014034/1965183‑1965374
|
aGATCACCTGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.C.CCC..c.c.c.c > 1:3578392/1‑101 (MQ=38)
aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCG..c.c.c.ccg > 2:3923592/1‑99 (MQ=255)
aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.G.CCG..c.c.c.ccc > 1:7067367/1‑99 (MQ=255)
cACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.c.c.cccgg > 1:749567/1‑97 (MQ=37)
ggCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGGGGC.T.CCG..c.c.c.cccgacccc > 1:6959951/1‑93 (MQ=25)
cACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.c.c.ccccccccccc > 1:538156/1‑91 (MQ=25)
acgacgATTTCTGTGGATGGCGGCTGGTCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.TACCGC.C.C.C.CGGAccccga > 2:3786489/1‑99 (MQ=25)
acgacgATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.C.C.CGGACCCCCgc > 2:3259254/1‑100 (MQ=37)
gttgtttgcTGTGGGGGGGGGGTGGGCGGCGCTGTGAGGTTTTCCGCCGGGGGGCAGG.CCCGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAgg < 2:4813308/93‑1 (MQ=2)
acgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.CCGCCC.C.C.CGGAccccccggg > 1:4109494/1‑97 (MQ=21)
aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.C.C.CGGCcccccccgggtt > 2:3678283/1‑94 (MQ=21)
aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.GCGC.C.C.C.CCGGCCCCCGAgcacgt > 2:2282712/1‑96 (MQ=21)
ttCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CCGGCCCCCGAGGGATTTc > 2:2108575/1‑101 (MQ=25)
tgtggggggggcggcGGGACGGCGGTGGGAGAGATTCCGCCGGGGGGCACC.CCGGGGGCGCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCa < 2:1663383/92‑1 (MQ=9)
cTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCGCCC..GGGGGC.C.CCC..C.C.C.CCGGCCCCCCCGGGAATTCAg > 2:2444425/1‑101 (MQ=21)
gtgGATGGCGGCTGTACGGCGCTGTGAGAGATTTCGCCTCGGGACAAG.CCCGAGGCGACCCGCCC..GGGGGG.C.CCGC.C.C.C.CGGCCCCCCGGGGAGTTTCAgc > 1:6289584/1‑100 (MQ=16)
gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGGAAG.CCCGAGGCGACCCG.CC..CGGGGCGC.CCC..C.C.C.CCGGCCCCCGAGGGGGTTCAGGc > 2:6601945/1‑101 (MQ=19)
gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCGC.C.C.C.CGGGCCCCCCCGGGATTTCAGGc > 2:6753129/1‑101 (MQ=21)
gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCaagg > 2:3279957/1‑98 (MQ=25)
tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCGCCC..GGGGGT.C.CCC..C.C.C.CCGGCCCCCCAGGGGTTTCAgggc > 1:7049404/1‑99 (MQ=16)
tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGGCCCCCGAGGGATTTCAGGCa > 2:6582998/1‑101 (MQ=25)
aTGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.G.CCGC.C.C.C.CGGACCCCCCGGGGATTTTAGGCAAGa > 2:3130774/1‑101 (MQ=21)
gggcggcGGGGCGGCGCGGGGAGAGTTTCCGCCGGGGGGCACC.CCCGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGAt < 1:5512047/100‑1 (MQ=9)
ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCAAGATGaaa > 2:1148386/1‑101 (MQ=21)
ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CCGGCCCCCGAGGGATTTCAGGCAAGATGaaa > 2:6997681/1‑101 (MQ=21)
ggggcgggCGCTGGGGGGGCTTCCGCCCCGGGCCACC.CCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAaga < 1:5668433/95‑1 (MQ=2)
cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAaga > 1:3332131/1‑101 (MQ=21)
cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCAAGATGAAaga > 1:1063692/1‑101 (MQ=21)
ggggcGGCGCTGGGAGGGATTCCGCGCGGGGGCACG.CCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAagag < 2:5528325/97‑1 (MQ=9)
ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.C.C.CGGCCCCCCCGGGGATTTTCGGCAGAATGaagagg > 2:6055765/1‑97 (MQ=9)
ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGGCCCCCCAGGGATTTCAGGCAAGATGAAAGAgg > 2:1966842/1‑101 (MQ=21)
ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.CCGC.C.C.C.CGGCCCCCCGGGGGATTTCAGGCAAGATGAAAGAgg > 2:1135338/1‑101 (MQ=21)
gggCGCGGGGAGAGGTGCCGCCGGGGGGGAAG.CCCGGGGCGCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGgc < 1:5970606/100‑1 (MQ=12)
cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.C.CCGC.C.C.C.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAggggg > 1:6749966/1‑100 (MQ=21)
cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAAAGGGgc > 2:2052166/1‑101 (MQ=21)
cTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAgggggggcca > 1:5379402/1‑95 (MQ=21)
cTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGGCCCCCGCGGGATTTCAGGCAAGATGAAAGAGGGGGGCTCa > 2:1104549/1‑101 (MQ=21)
tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGCCC.C.C.CGGACCCCC.AGGGATTTCAGGGAAGATGAAAGAGGGgggcgccg > 2:5590010/1‑97 (MQ=17)
tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAg > 1:2814987/1‑101 (MQ=21)
tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.ACGC.C.C.C.CCGACCCCCGAGGTATTTCAGGCAAGATGCAAGAGGGgcgcggag > 2:4226006/1‑97 (MQ=21)
gtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.C.C.CCGGCCCCCCAGGGATTTCAGGCAAGATGAAAAAGGGGGGCTCAgg > 1:6693854/1‑101 (MQ=18)
tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCGC.C.C.C.CGGACCCCCCAGGGCATTCAGGAAAAAAGAAAAAAGGGGGGTCCggc > 2:6207568/1‑101 (MQ=9)
tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCCAGATGAAAGAGGGGCGCTCCggg > 2:4330509/1‑100 (MQ=21)
tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGGGGC.T.CCG..CGC.C.CGGACCCCCGAGGGATTTCACGCTAAGTGAAAGATGGGGGCTctcga > 1:1201134/1‑97 (MQ=16)
gagagaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcg > 1:1122943/1‑101 (MQ=21)
agagaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGCGGC.T.CCGC.C.C.C.CGGCCACCCGCGGTTTTTGCGGGAAGATGAAAGAGGGGCGCTCCggccg > 1:3070346/1‑99 (MQ=16)
agaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcgggc > 2:2265389/1‑99 (MQ=21)
ttcgcCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGGCCCCCCAGGGATTTCCGGCAAGATGAAAGAGGGGCGCTCAGGGGGCcttct > 1:3203575/3‑101 (MQ=21)
cccccggggggCAAGCCCCGGGCCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCcttct < 2:5922423/94‑1 (MQ=18)
gcCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.TCCCC..C.C.C.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCttcttcc > 2:1645568/1‑100 (MQ=25)
gggggcCAGG.CCCGCGGCCCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgtcg < 1:7001062/99‑1 (MQ=18)
ggggCAAG.CCCGAGGCGACCCG.CC..GGGGGG.TCCCC..C.C.C.CCGGCCCCCCAGGGATTTCAGGCAAAATGAAAGAAGGGGGCTCCGGCGGGCCTCTTCtccccc > 2:1956425/1‑101 (MQ=12)
ggggCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCgccgcc > 2:3301308/1‑101 (MQ=255)
gggCAAG.CCCGAGGCGACCCG.CCG.GGGGGC.C.CCC..C.C.C.CCGGCCCCCCAGGGGTTTCCGGCAAGATGAAAGAGGGGCGCTCCGGCGGGCCTCTTCgccgccc > 2:5456036/1‑100 (MQ=19)
ggCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGCCC.C.C.CGGACCCCC.AGGGATTTCAGGCAAGATGCAAGAGGGGCGCTCAGGCGGCCCTCTTCgccgccct > 1:6737006/1‑99 (MQ=21)
gccccccagAGGCGCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTg < 1:4226201/93‑1 (MQ=21)
aaG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.C.C.CCGGCCCCCCCGGGATTTTCGGGCAGAAGGAAGAGGGGGGCGCCGGGGGGCCTCTTCGCCCCCCTGcc > 1:1672209/1‑101 (MQ=0)
ccgcgcggCGCCCCG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCg < 2:4632253/95‑1 (MQ=25)
ccggcgcccccccG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCgg < 2:7012487/91‑1 (MQ=25)
cggggcccccccG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgt < 1:4330731/92‑1 (MQ=25)
cGAGGCGACCCG.CC..GGGGGC.TCCCC..C.C.C.CCGACCCCCGAGGGATTTCAGGCCAGAAGAAAGAAGGGGGCCCAGGCGGGCCTCTTCTCCGCCCTGccccggg > 2:2743228/1‑97 (MQ=12)
gcgcccccccG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtgt < 2:2081807/94‑1 (MQ=25)
gAGGCGACCCG.CC..GGGGGC.TCCCC..C.C.C.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCCGGCGGCCCTCCTCCGCGCCCTGCCCggggc > 2:6781701/1‑98 (MQ=12)
cccccccG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc < 1:7017370/97‑1 (MQ=25)
ccccG.CC..GGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg < 1:4682194/100‑1 (MQ=255)
ccccG.CC..GGGGGC.T.CCG..C.C.CGCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg < 1:5607254/100‑1 (MQ=255)
ccg.cc..gGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct < 1:2490659/101‑1 (MQ=255)
ccg.cc..gGGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct < 1:60196/101‑1 (MQ=255)
cg.cc..ggGGGC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt < 2:4999147/101‑1 (MQ=255)
cgggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttctt < 2:6866606/98‑1 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGGCCGGTGTCTCGGGCTTCTTg > 1:4376058/3‑101 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg > 2:4448708/3‑101 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg > 1:5424354/3‑101 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg > 1:7056432/3‑101 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg > 2:5813367/3‑101 (MQ=255)
gggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg < 1:99470/99‑1 (MQ=255)
gggggC.T.CCG..C.CGC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTg > 1:4143678/3‑101 (MQ=255)
ggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGt > 1:1973075/2‑101 (MQ=255)
ggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGt > 1:7097575/2‑101 (MQ=255)
ggggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGt > 1:5256579/2‑101 (MQ=255)
gggC.T.GCGC.C.C.C.CGGGCCCCCGAGGTATTTCAGGCAATATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTc > 1:6856674/1‑101 (MQ=255)
gggC.T.CCGC.C.C.C.CGGACCCCCGAGGCATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTc > 1:4110734/1‑101 (MQ=255)
ggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCg < 2:6605791/101‑1 (MQ=255)
ggC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCg < 2:311260/101‑1 (MQ=255)
ggC.T.CCGC.C.C.C.CGGACCCCCGAGGCATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTCCGCCGCCTCGGCCGGTGGCTCTGCCTTCTTGTCg > 2:1183511/1‑101 (MQ=25)
gC.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCgg > 1:6614943/1‑101 (MQ=255)
c.T.CCGC.C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCGGc > 2:5636074/1‑101 (MQ=255)
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AGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.C.C.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCTTGTCGGC > NC_014034/1965183‑1965374
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 15 ≤
ATCG
< 25 ≤
ATCG
< 33 ≤
ATCG
< 41 ≤
ATCG
|