Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,326 +G 100% intergenic (‑19/+36) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3261.→G100.0% 1283.5 343intergenic (‑19/+36)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (173/167):  ref base (0/1):  total (173/170)

Only 100 of 392 total aligned reads displayed.

TGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.GGGC.GGGGT.GT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974226‑1974419
                                                                                                      |                                                                                                
tGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGtt                                                                                                   >  2:2433929/1‑101 (MQ=255)
 ggCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGCTCGAGGGGGCGATCTTGATGGAACGGACGAAGGTCATGTCGGTCTCCTGA.GGGGGT.g                                                                                                 >  1:735449/1‑101 (MQ=255)
 ggCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.g                                                                                                 >  1:3386238/1‑101 (MQ=255)
 ggCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.g                                                                                                 >  2:6743906/1‑101 (MQ=255)
    cTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.gggg                                                                                              >  1:4946962/1‑100 (MQ=255)
      ggATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.ggggcg                                                                                            >  1:2925006/1‑98 (MQ=255)
       gATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.ggggcgg                                                                                           >  1:1138516/1‑97 (MQ=255)
         tttCACGGCCGAAATCGGTGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGC.gggg                                                                                         <  2:5867148/101‑1 (MQ=255)
         tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGGCATGTCGGTCGCCTGA.GGGGTTGGGGC.gggg                                                                                         >  1:4360804/1‑101 (MQ=255)
           tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGC.gggggg                                                                                       >  2:1118818/1‑99 (MQ=255)
           tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGCCATGTCGGTCTCCTGA.GGGGTTGGGGC.gggggg                                                                                       >  2:664997/1‑99 (MQ=255)
            cACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGAGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGC.ggggggg                                                                                      >  2:2230119/1‑98 (MQ=255)
             aCGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGC.gggggtgt                                                                                     <  2:1398810/101‑5 (MQ=255)
                 ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GT.cccc                                                                                <  1:3330542/101‑1 (MQ=255)
                  cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GT.ccccc                                                                               <  2:2008229/101‑1 (MQ=255)
                   gAAATCGTCGAAGTCGGCAGAAAGTATCGACGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GT.cccccc                                                                              <  1:3103601/101‑2 (MQ=255)
                   gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GT.cccccc                                                                              >  2:4569355/1‑100 (MQ=255)
                     aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GT.ccccccgc                                                                            <  2:5890227/101‑4 (MQ=255)
                      aTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GT.ccccccgcc                                                                           >  2:3940739/1‑97 (MQ=255)
                        cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCcccgcccg                                                                          <  2:638786/101‑1 (MQ=255)
                           cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGAt                                                                       <  2:1289069/101‑1 (MQ=255)
                           cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGAt                                                                       <  1:1028022/101‑1 (MQ=255)
                            gAAGTCGGCAGAAAGGGTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGGGG.GGGCGGGGGTGGC.CCCCCGCCCGGATa                                                                      >  2:7053654/1‑101 (MQ=255)
                            gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATa                                                                      >  1:7012992/1‑101 (MQ=255)
                             aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAg                                                                     <  1:5551839/101‑1 (MQ=255)
                             aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAg                                                                     >  1:5416396/1‑101 (MQ=255)
                              aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGc                                                                    <  1:1647327/101‑1 (MQ=255)
                              aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGc                                                                    <  1:949386/101‑1 (MQ=255)
                              aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGc                                                                    >  1:4247021/1‑101 (MQ=255)
                              aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGc                                                                    >  2:3007898/1‑101 (MQ=255)
                               gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCa                                                                   >  2:3124606/1‑101 (MQ=255)
                               gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCa                                                                   <  1:4161361/101‑1 (MQ=255)
                                 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCAcg                                                                 <  1:6040424/101‑2 (MQ=255)
                                 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGagacgccg                                                                 >  2:2304835/1‑94 (MQ=255)
                                 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCAcg                                                                 >  2:118990/1‑100 (MQ=255)
                                   gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCAcggc                                                               <  2:4687749/101‑4 (MQ=255)
                                    cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCAcggcg                                                              >  2:4610681/1‑97 (MQ=255)
                                     aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGt                                                            >  2:4039294/1‑101 (MQ=255)
                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          >  1:2407445/1‑101 (MQ=255)
                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          >  1:5126532/1‑101 (MQ=255)
                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          <  1:2257531/101‑1 (MQ=255)
                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          <  1:2304930/101‑1 (MQ=255)
                                       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCA.CGGCGTTc                                                          >  1:6915634/1‑101 (MQ=255)
                                           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  1:3878206/101‑1 (MQ=255)
                                           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  2:6129341/101‑1 (MQ=255)
                                           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  1:2686818/101‑1 (MQ=255)
                                            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                     >  1:831367/1‑100 (MQ=255)
                                            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                     >  2:3716551/1‑100 (MQ=255)
                                              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                   <  2:4285146/101‑1 (MQ=255)
                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGttcccg                                                  >  2:4988790/1‑97 (MQ=255)
                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  2:2680601/1‑101 (MQ=255)
                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:5302125/1‑101 (MQ=255)
                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:5899390/1‑101 (MQ=255)
                                               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCCTTCAGTTCACg                                                  >  2:2877642/1‑101 (MQ=255)
                                                aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                 >  1:5639184/1‑101 (MQ=255)
                                                 gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                <  2:5301859/101‑1 (MQ=255)
                                                  ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                               <  2:336241/101‑1 (MQ=255)
                                                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                              <  2:5956944/101‑1 (MQ=255)
                                                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                              >  2:1143103/1‑101 (MQ=255)
                                                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             <  1:1203992/101‑1 (MQ=255)
                                                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             <  1:5037416/101‑1 (MQ=255)
                                                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             >  2:865029/1‑101 (MQ=255)
                                                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                            <  1:1848130/101‑1 (MQ=255)
                                                       gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          >  2:1562335/1‑101 (MQ=255)
                                                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagagga                                         >  1:5210979/1‑99 (MQ=255)
                                                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                         >  1:2561170/1‑101 (MQ=255)
                                                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                         >  1:1470274/1‑101 (MQ=255)
                                                           ttGATGGAACGGTCGACGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      <  1:5866272/101‑1 (MQ=255)
                                                           ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      >  1:1993337/1‑101 (MQ=255)
                                                           ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      >  1:5745803/1‑101 (MQ=255)
                                                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                     >  2:3520145/1‑101 (MQ=255)
                                                             gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                    >  2:3891284/1‑101 (MQ=255)
                                                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                   >  2:6218734/1‑101 (MQ=255)
                                                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 >  1:632232/1‑101 (MQ=255)
                                                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                >  2:4460158/1‑101 (MQ=255)
                                                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                >  2:5149703/1‑101 (MQ=255)
                                                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                               >  2:5540950/1‑101 (MQ=255)
                                                                   aCGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                              <  1:6623877/101‑1 (MQ=255)
                                                                    tccgacggggTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                             >  2:2168043/9‑101 (MQ=255)
                                                                     ggTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                            <  2:4724035/101‑1 (MQ=255)
                                                                     ggTCGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                            >  1:3015911/1‑101 (MQ=255)
                                                                        cGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         <  1:129036/101‑1 (MQ=255)
                                                                        cGAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         >  1:21703/1‑101 (MQ=255)
                                                                         gAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        <  2:3757866/101‑1 (MQ=255)
                                                                         gAAGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGG.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        >  2:4189658/1‑101 (MQ=255)
                                                                           aGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                      <  1:4569594/101‑1 (MQ=255)
                                                                           aGGTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                      >  2:6893289/1‑101 (MQ=255)
                                                                            ggTCATGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                     <  1:4734648/101‑1 (MQ=255)
                                                                                aTGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                 <  2:5766850/101‑1 (MQ=255)
                                                                                 tGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTgc                >  2:761678/1‑100 (MQ=255)
                                                                                 tGTCGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                >  2:1895813/1‑101 (MQ=255)
                                                                                    cGGTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCgg             <  1:6427499/101‑1 (MQ=255)
                                                                                     ggTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc            <  2:4423631/101‑1 (MQ=255)
                                                                                     ggTCTCCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc            >  2:4742729/1‑101 (MQ=255)
                                                                                         tcCTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        >  2:5668189/1‑101 (MQ=255)
                                                                                          ccTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt       <  1:6219053/101‑1 (MQ=255)
                                                                                           cTGA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      <  2:3576696/101‑1 (MQ=255)
                                                                                             gA.GGGGTTGGGGCGGGGGT.GTCCCCCCGCCCGGGTAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg    >  1:3708747/1‑101 (MQ=255)
                                                                                                ggggTTGGGGCGGGGGT.GTCCCCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  >  1:4469764/1‑101 (MQ=255)
                                                                                                ggggTTGGGGCGGGGGT.GTCCCCCCGCCCGGACAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  <  1:4858438/101‑1 (MQ=255)
                                                                                                      |                                                                                                
TGGCCTGGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGA.GGGGTT.GGGC.GGGGT.GT.CCCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974226‑1974419

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG