Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,979,921 +G 2.0% intergenic (‑37/+47) tkt1 ← / ← cbbP transketolase/phosphoribulokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,979,9211.→G 2.0% 9.5 ‑4.3 ‑0.1302intergenic (‑37/+47)tkt1/cbbPtransketolase/phosphoribulokinase
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (144/152):  total (150/152)
Fisher's exact test strand distribution p-value = 1.43e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.24e-01

Only 100 of 338 total aligned reads displayed.

CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021
                                                                                                     |                                                                                                      
ccatcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAgcggc                                                                                                         >  1:7091944/1‑101 (MQ=255)
    cGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.c                                                                                                   <  2:4377354/101‑1 (MQ=255)
     gCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.cc                                                                                                  <  1:5298896/101‑1 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ccc                                                                                                 <  1:6628455/101‑1 (MQ=255)
       cAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCg                                                                                                <  1:1870902/101‑1 (MQ=255)
         gcgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCggg                                                                                              >  2:6295080/1‑100 (MQ=255)
          cgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTg                                                                                             <  1:2652834/101‑1 (MQ=255)
           gcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.ccccggcg                                                                                            >  1:4436350/1‑96 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggcc                                                                                         <  1:694572/101‑1 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggcc                                                                                         <  2:4781618/101‑1 (MQ=255)
               ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGG.CCCGGGgggcg                                                                                         >  2:2071891/1‑100 (MQ=255)
               ggATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.GCCCGGgggccg                                                                                        >  2:512304/1‑101 (MQ=255)
                 aTGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGggggcggg                                                                                      >  1:3103575/1‑101 (MQ=255)
                   ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTgggccgggcc                                                                                    <  1:603698/101‑1 (MQ=255)
                    gCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGgggccgggcc                                                                                    >  2:1641319/1‑101 (MQ=255)
                    gCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCt                                                                                   <  1:1789424/101‑1 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTc                                                                                  <  2:2957972/101‑1 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTc                                                                                  <  1:28466/101‑1 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGc                                                                                <  1:6361423/101‑1 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGc                                                                                <  1:4500332/101‑1 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGTgggccgggcccccc                                                                                >  1:5812859/1‑97 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCGCGGCCTccc                                                                                >  2:6602849/1‑99 (MQ=255)
                         ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCa                                                                              <  2:192794/101‑1 (MQ=255)
                           ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAAt                                                                            <  2:4433432/101‑1 (MQ=255)
                           ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGCC.GGG.CCCGGTGGGCCGGGCCTCGCCAAt                                                                            <  1:6283574/101‑1 (MQ=255)
                               ccGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGGCTCGCCCATGTC.t                                                                       >  2:32762/1‑101 (MQ=255)
                                cgcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.tt                                                                      <  1:159816/101‑1 (MQ=255)
                                cgcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.tt                                                                      <  2:1990591/101‑1 (MQ=255)
                                cgcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.tt                                                                      <  2:6353172/101‑1 (MQ=255)
                                     ttCCTGCGCCATAGCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAAt                                                                 <  2:4044302/101‑1 (MQ=255)
                                       ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGCCTCGCCCATGTC.ttagatcgg                                                               >  1:2408080/1‑94 (MQ=255)
                                       ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTACCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCg                                                               <  1:3371116/101‑1 (MQ=255)
                                        cTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGt                                                              <  1:5472258/101‑1 (MQ=255)
                                        cTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGt                                                              <  2:3076619/101‑1 (MQ=255)
                                        cTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCCACGTC.CTTaaaatgt                                                              >  2:6851793/1‑97 (MQ=255)
                                         cgcgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGtt                                                             <  2:2825895/100‑1 (MQ=255)
                                           cgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTcc                                                           <  2:1013865/101‑1 (MQ=255)
                                           cgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTcc                                                           <  1:5124643/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCg                                                          <  2:391951/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCg                                                          <  2:4719499/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCg                                                          <  2:554488/101‑1 (MQ=255)
                                             ccATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCgg                                                         <  2:5011871/101‑1 (MQ=255)
                                             ccATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCgg                                                         <  2:2133940/101‑1 (MQ=255)
                                             ccATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGCCTCGCCAACGGC.TTGAAAACGTTcccg                                                         >  2:4293493/1‑99 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGGCGGGCCCCGCCCATGTC.TTTGAATCCTTCCCGt                                                        >  1:3903565/1‑101 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGGCCCGCCCATGTCTTTGAAATCTTCCCgg                                                         >  2:5293703/1‑101 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGGCCCGCCCATGTC.TTTAAAACGTTCCGGtt                                                       >  1:2000870/1‑101 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGCCCCGCCCATGTC.TTTAAATCCTTCCgggt                                                       >  1:6706178/1‑99 (MQ=255)
                                                tatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTc                                                      <  1:2110314/101‑1 (MQ=255)
                                                tatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGGCCCGCCCATTGT.TTTAAAACGGTCCgggtt                                                      >  2:3506359/1‑98 (MQ=255)
                                                tatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGGCTCGCCCATGTC.TTTAAATCCTTCCGGTTc                                                      >  1:6162791/1‑101 (MQ=255)
                                                   cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAgg                                                   <  1:4960426/101‑1 (MQ=255)
                                                     agaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGCCTCGCCCATGTC.TTTGAAACGTTCCGGTTCAgggc                                                 >  2:28665/1‑99 (MQ=255)
                                                     agaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGCCTCGCCAATGTC.TTTAAATCGTTTCGGGTCAGGcc                                                 >  2:5637807/1‑101 (MQ=255)
                                                       aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGCCCCCCCCAAGTC.TTTAAACTGTTCCGGGTTAGGcccc                                               >  1:3431042/1‑100 (MQ=255)
                                                           ttCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCcgcgcg                                           <  2:3037905/101‑1 (MQ=255)
                                                           ttCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGCGGGCGGGACCCGCCCCACTC.TTGAACACGGCCCGGGTCAGGGCcacgcg                                           >  2:4951396/1‑101 (MQ=255)
                                                            tCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCCATGTC.TTGAAATCGTTCCGGGTTAGGCCcccgcgc                                          >  1:4920370/1‑101 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGTGGGCCGGGCCTCGCCAATGTC.TTTAAAACGTTCCGGGTGACGCCcccgcggcg                                        >  2:1206001/1‑98 (MQ=255)
                                                               tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGAc                                       <  1:4991402/101‑1 (MQ=255)
                                                               tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGAc                                       <  1:426280/101‑1 (MQ=255)
                                                               tGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGAc                                       <  1:1206049/101‑1 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCCATGGC.TTGAAATCGTTCCGGGTTAGGCCCGCGCGCGACg                                      >  1:2701635/1‑101 (MQ=255)
                                                                  ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGc                                    <  1:32763/101‑1 (MQ=255)
                                                                  ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCCGGGGGCCGGGCCTCGCCCATGTC.TTGAAATCGTTCCGGGTCAGGCCCGCGCGCGGCGGc                                    >  1:6501/1‑101 (MQ=255)
                                                                     ctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCtt                                 <  2:1388284/101‑1 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCTTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  2:5771995/101‑1 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  2:2428228/101‑1 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  1:4031969/101‑1 (MQ=255)
                                                                       cctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTcg                               <  1:4590563/101‑1 (MQ=255)
                                                                       cctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTcg                               >  2:3188141/1‑101 (MQ=255)
                                                                        ctccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTcgc                              <  1:4400964/101‑1 (MQ=255)
                                                                        ctccCTGATGAGCCTTTCGTCCGAACGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTcgc                              <  2:7033148/101‑1 (MQ=255)
                                                                         tccCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCCATGTC.TTGAAATCGTTCCGGTTCAGGGCCGCGCGCGGCGGCCTTcgcg                             >  2:5682157/1‑101 (MQ=255)
                                                                          cccTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCgcgc                            <  2:6669866/101‑1 (MQ=255)
                                                                          cccTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGGCGGGCCTCGCCCATGTC.TTTAAATCGTTCCGGTTCAGGCCCGCGCGCGGCGGGCTTCccgc                            >  1:2185699/1‑101 (MQ=255)
                                                                          cccTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCgcgc                            >  1:157668/1‑101 (MQ=255)
                                                                            cTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAc                          <  1:4859313/101‑1 (MQ=255)
                                                                            cTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGGCGGCCTTCGCGCAc                          >  1:3864102/1‑101 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAcc                         <  1:1478696/101‑1 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAcc                         >  2:6868941/1‑101 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCCGGCCTCGCCAATGTC.TTTAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAcc                         >  2:2527774/1‑101 (MQ=255)
                                                                              gatgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCa                        <  2:6245856/101‑1 (MQ=255)
                                                                               ttccgatcttCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAg                       <  2:3891524/93‑1 (MQ=255)
                                                                                tgaGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCCCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGGCTTCGCGCCcccca                      >  1:4165339/1‑98 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGGC.GGG.CCCCGTGGGCCGGGCCCCGCCAATGTC.TTGAAATCGTTCCGGCTCAGGCCCCGGCGCGGCGGCCTTCGCGCACCAGAc                     >  2:6748805/1‑101 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGGC.GGG.CCCCGTGGGCCGGGCCCCGCCAATGTC.TTGAAATCGTTCCCGGTCAGGCCCCCGCGCGGCGGACTTtgcggccccgac                     >  2:720411/1‑93 (MQ=255)
                                                                                  aGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCCATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGGCGGCCTTCGCGCACCAGACg                    >  1:565095/1‑101 (MQ=255)
                                                                                  aGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGGCGGCCCTCGCGCACCCGACg                    >  2:3637370/1‑101 (MQ=255)
                                                                                   gcttttCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGc                   <  2:4424171/98‑1 (MQ=255)
                                                                                    ccTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCt                  <  2:4901969/101‑1 (MQ=255)
                                                                                    ccTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCCCGCCAATGTC.TTGAAAACGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCCGACGCt                  >  1:6868917/1‑101 (MQ=255)
                                                                                     cTTTCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGCCGGCCTTCGCGCACCAGACGCTc                 >  2:540459/1‑101 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGGCTCGCCCATGTC.TTGAAATCGTTCCGGTTCAGGGCCGCGCGCGACGGCCTTCGCGCACCCGACGCTcc                >  2:6061577/1‑100 (MQ=255)
                                                                                        tCGTCCGAGCGGC.GGG.CCCGGGGGGCCGGGCCCCGCCAATGTC.TTGAAATCGTTCCGGGTCAGGCCCGCGCGCGGCGGCCTTCGCGCCCCAGACGCTCGAt              >  1:4749858/1‑101 (MQ=255)
                                                                                          gTCCGAGCGGC.GGG.CCCGGTGGTCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCa            <  2:2645508/101‑1 (MQ=255)
                                                                                              gAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcgg        >  1:3641460/1‑101 (MQ=255)
                                                                                                 cggc.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcggcgg     >  1:1081206/1‑100 (MQ=255)
                                                                                                  ggc.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    >  1:7132041/1‑101 (MQ=255)
                                                                                                    c.ggG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAg  >  1:1347417/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGG.CCCGGTGGGCCGGGCCTCGCCAATGTC.TTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG