BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,997,981	+G	97.8%	intergenic (‑46/‑119)	RCAP_rcc01844 ← / → RCAP_rcc01845	sensor histidine kinase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,997,981	1	.→G	97.8%	16.8 ‑1.1 ‑1.0	406	intergenic (‑46/‑119)	RCAP_rcc01844/RCAP_rcc01845	sensor histidine kinase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (206/191): ref base (3/6): total (209/197)
Fisher's exact test strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.05e-01

Only 100 of 443 total aligned reads displayed.


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTTCC  >  NC_014034/1997881‑1998078


gCAGAATCGGAAACGGTAACGTCTCGGCCAATGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTc                                                                                                    <  1:3881227/101‑1 (MQ=255)


 cAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGACACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.g                                                                                                  >  2:4516087/1‑101 (MQ=255)


  aGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGCTGACGAATCCGCCATTTTTCGGGCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gg                                                                                                 >  2:4779232/1‑101 (MQ=255)


    aaTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gggg                                                                                               >  2:4119271/1‑100 (MQ=255)


     aTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.gggga                                                                                              >  1:6931741/1‑99 (MQ=255)


        ggAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaaac                                                                                           <  2:3599322/101‑3 (MQ=255)


        ggAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGgaaac                                                                                           <  2:6208935/101‑3 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACg                                                                                           >  2:220845/1‑101 (MQ=255)


          aaaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGa                                                                                          >  2:292403/1‑101 (MQ=255)


          aaaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGa                                                                                          >  1:1559171/1‑101 (MQ=255)


           aaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGAt                                                                                         <  1:5372592/101‑1 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAc                                                                                       >  2:3244874/1‑101 (MQ=255)


              ggTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAcc                                                                                      <  1:2496530/101‑1 (MQ=255)


               gTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATAccc                                                                                     <  1:275666/101‑1 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCa                                                                                    <  2:5961855/101‑1 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCaa                                                                                   >  1:5728897/1‑101 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCaa                                                                                   <  1:1159316/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                                 >  2:1119897/1‑101 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                                 >  1:4657517/1‑101 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGt                                                                                 >  2:6096161/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGtt                                                                                >  2:1323902/1‑101 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGtt                                                                                >  1:1243925/1‑101 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTc                                                                               >  2:1489065/1‑101 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTc                                                                               <  1:5867668/101‑1 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTc                                                                               >  1:3375095/1‑101 (MQ=255)


                     tctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTc                                                                               >  2:264932/1‑101 (MQ=255)


                      cccGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCa                                                                              <  2:6813257/99‑1 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCg                                                                        <  2:3683064/101‑1 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                      >  2:7134011/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCa                                                                      >  1:2985312/1‑101 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCAt                                                                     <  2:1161908/101‑1 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCAt                                                                     <  2:2407091/101‑1 (MQ=255)


                                gagaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCCAGTTCAAGGCCGCATg                                                                    >  1:5297546/1‑101 (MQ=255)


                                gagaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATg                                                                    <  1:4560552/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                                 <  1:7106331/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                                 <  1:5573001/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                                 >  1:568577/1‑101 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                                 >  2:5081451/1‑101 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                                <  1:3724286/101‑1 (MQ=255)


                                    ttgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaa                                                                >  1:6880711/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                               >  2:3976967/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                               >  1:2826567/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                               >  1:1748014/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                               >  1:2237452/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                              >  1:2036439/1‑101 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                              >  1:4873802/1‑101 (MQ=255)


                                         gccGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCa                                                           <  2:5447309/99‑1 (MQ=255)


                                         gACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCa                                                           <  1:19621/101‑1 (MQ=255)


                                            gAATCCGCCATTTTTCGGTCACCCTGACATATCCAATTCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGNNNggg                                                        >  1:2323642/1‑101 (MQ=255)


                                            gAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggg                                                        >  1:3585094/1‑101 (MQ=255)


                                              aTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCGAAGTTCAAGGCCGCATGCAAAAGCCAggggg                                                      >  1:6330893/1‑101 (MQ=255)


                                              aTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggggg                                                      >  2:3423705/1‑101 (MQ=255)


                                                ccGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGc                                                    >  2:6666259/1‑101 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                  >  2:1445427/1‑101 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                  <  1:285594/101‑1 (MQ=255)


                                                  gCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                  >  1:6028029/1‑101 (MQ=255)


                                                   ccATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTaa                                                 <  2:5543110/101‑1 (MQ=255)


                                                   ccATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTaa                                                 <  2:3797981/101‑1 (MQ=255)


                                                    cATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                                <  2:3299640/101‑1 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                                >  1:5126411/1‑101 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                           <  2:1895478/101‑1 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                           <  2:6439508/101‑1 (MQ=255)


                                                          tCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTg                                          >  1:4220751/1‑101 (MQ=255)


                                                           cGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGc                                         >  1:3097586/1‑101 (MQ=255)


                                                           cGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGc                                         <  1:3472309/101‑1 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                        <  2:2443130/101‑1 (MQ=255)


                                                            ggTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCt                                        >  1:83550/1‑101 (MQ=255)


                                                             gTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTg                                       >  1:5952906/1‑101 (MQ=255)


                                                             gTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTg                                       <  1:1452397/101‑1 (MQ=255)


                                                              tccgatctaCATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGa                                      <  2:4672350/93‑1 (MQ=255)


                                                              tCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGa                                      <  1:1353525/101‑1 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                     >  1:5824273/1‑101 (MQ=255)


                                                                aCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAt                                    >  1:4350226/1‑101 (MQ=255)


                                                                aCCCTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCAGCCAAAAGCCAGGGGGGATGATCTTTTGCTGagt                                    >  2:5692888/1‑99 (MQ=255)


                                                                 cccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCTGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAAtt                                   >  1:5093757/1‑101 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                  >  1:4025564/1‑101 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                  <  2:5728595/101‑1 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                  <  1:917845/101‑1 (MQ=255)


                                                                   cTGACATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcg                                 >  1:1009919/1‑101 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                              >  2:1589310/1‑101 (MQ=255)


                                                                       cATATCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGa                             >  1:4512346/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                            >  1:2392999/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                            >  2:5589853/1‑101 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                          <  2:4247026/101‑1 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                          >  1:2949830/1‑101 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                          >  1:6134237/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                       <  1:1323950/101‑1 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                       >  2:6329819/1‑101 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                     <  1:6257443/101‑1 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                   <  1:3180770/101‑1 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                   <  2:6257221/101‑1 (MQ=255)


                                                                                  cTTGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCa                  >  2:4291558/1‑101 (MQ=255)


                                                                                   ttGACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAt                 >  1:644701/1‑101 (MQ=255)


                                                                                     gACCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGa               >  2:1691590/1‑101 (MQ=255)


                                                                                      aCCCGTGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGGTGTTGTCATGAg              >  2:5841434/1‑101 (MQ=255)


                                                                                      aCCCGAGCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAg              <  2:3115936/101‑1 (MQ=255)


                                                                                           ggCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATg         <  2:3012211/100‑1 (MQ=255)


                                                                                            gCAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATgg        <  2:203616/101‑1 (MQ=255)


                                                                                             cAAGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATggg       >  1:6644516/1‑101 (MQ=255)


                                                                                               aGCTTCGGGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGtt     <  2:85766/101‑1 (MQ=255)


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTC.GGGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTTCC  >  NC_014034/1997881‑1998078

Base quality scores:


ATCG

< 3 ≤


ATCG

< 26 ≤


ATCG

< 32 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG