Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,155 +C 3.6% intergenic (+28/+149) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,1550.→C 3.6% 32.2 0.0 ‑0.7357intergenic (+28/+149)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (154/190):  total (160/197)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.11e-01

Only 100 of 406 total aligned reads displayed.

GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGC  >  NC_014034/2044055‑2044251
                                                                                                                                                                                                     
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtt                                                                                                 <  2:5232228/101‑1 (MQ=255)
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtt                                                                                                 <  1:173195/101‑1 (MQ=255)
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtt                                                                                                 <  2:5058218/101‑1 (MQ=255)
  cGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttt                                                                                                <  2:1403236/101‑1 (MQ=255)
   gAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttt                                                                                               >  1:3901707/1‑101 (MQ=255)
   gAAGATCGTGCGTCGGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttt                                                                                               <  2:630905/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                              >  1:5501987/1‑101 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCttttt                                                                                              <  1:4978362/101‑1 (MQ=255)
     aGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttc                                                                                             <  1:1002970/101‑2 (MQ=255)
       aTCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttcat                                                                                           >  1:6702296/1‑98 (MQ=255)
        tCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttcatt                                                                                          <  2:6589710/101‑5 (MQ=255)
        tCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCtttttcatt                                                                                          <  2:7090770/101‑5 (MQ=255)
         cGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTc                                                                                        >  2:3624038/1‑101 (MQ=255)
         cGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTc                                                                                        <  1:342020/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                       <  1:4176655/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                       >  1:6253364/1‑101 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                       <  2:4803293/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                       >  1:2325305/1‑101 (MQ=255)
           tGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGt                                                                                      <  2:6424359/101‑1 (MQ=255)
            gCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTg                                                                                     <  2:2960707/101‑1 (MQ=255)
                cTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGa                                                                                 >  1:5701373/1‑101 (MQ=255)
                cTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGa                                                                                 >  2:2389921/1‑101 (MQ=255)
                  gcgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAAt                                                                               <  2:5476328/101‑1 (MQ=255)
                   cgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATg                                                                              <  2:3405227/101‑1 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                             <  1:1923164/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                            <  1:3851004/101‑1 (MQ=255)
                      aaGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaaa                                                                           <  1:2212979/101‑1 (MQ=255)
                      aaGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaaa                                                                           >  2:252942/1‑101 (MQ=255)
                         cGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTc                                                                        <  2:3261005/101‑1 (MQ=255)
                          ggCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                       <  1:6758103/101‑1 (MQ=255)
                             ttCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCt                                                                    >  2:4106831/1‑101 (MQ=255)
                             ttCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCt                                                                    >  1:1401212/1‑101 (MQ=255)
                              tCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTc                                                                   >  1:4691070/1‑101 (MQ=255)
                               cTGGACCCGCACGAGCGCAATCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                  <  1:5625686/101‑1 (MQ=255)
                                 ggACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccc                                                                >  2:5374519/1‑101 (MQ=255)
                                  gACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTccccc                                                               <  2:4246110/101‑1 (MQ=255)
                                   aCCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcccccc                                                              >  2:5144906/1‑101 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                             <  2:729522/101‑1 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                             <  1:2282914/101‑1 (MQ=255)
                                       gCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGa                                                          <  1:2882671/101‑1 (MQ=255)
                                        cACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGat                                                         >  1:4452220/1‑101 (MQ=255)
                                        cACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGat                                                         <  2:2827688/101‑1 (MQ=255)
                                         aCGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGata                                                        <  2:6115542/101‑1 (MQ=255)
                                           gAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAtt                                                      >  2:1201763/1‑101 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                     <  1:5348862/101‑1 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                     <  1:2390015/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGGCGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                    <  2:1805960/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                    <  1:3624187/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                    <  2:4875955/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGACGACAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                    <  2:3901544/101‑1 (MQ=255)
                                              cncAAACGCGCCTCGCGNTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGa                                                   <  2:229022/101‑1 (MQ=255)
                                               gcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAg                                                  >  1:7017511/1‑101 (MQ=255)
                                               gcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAg                                                  <  2:6752409/101‑1 (MQ=255)
                                                 aaaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGc                                                <  2:7005650/101‑1 (MQ=255)
                                                  aaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCa                                               <  2:4496350/101‑1 (MQ=255)
                                                  aaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCa                                               <  2:1577770/101‑1 (MQ=255)
                                                  aaCGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCa                                               <  2:1092982/101‑1 (MQ=255)
                                                    tgcttcTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAg                                             <  2:5266787/96‑1 (MQ=255)
                                                     gcgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGa                                            <  2:1318231/101‑1 (MQ=255)
                                                      cgcCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGAt                                           <  2:3852601/101‑1 (MQ=255)
                                                      ccgatctcgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGAt                                           <  1:4887655/94‑1 (MQ=39)
                                                        ccTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTGGAGGCAAGATaa                                         <  1:6320934/101‑1 (MQ=255)
                                                             cgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACt                                    <  1:1587565/101‑1 (MQ=255)
                                                             cgcTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACt                                    <  2:6222172/101‑1 (MQ=255)
                                                              gcgcGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                   <  1:3311264/98‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                  <  2:857448/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                  >  1:4022242/1‑101 (MQ=255)
                                                                tCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGc                                 <  1:1697077/101‑1 (MQ=255)
                                                                tCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGc                                 >  2:4533385/1‑101 (MQ=255)
                                                                 cGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                >  1:6376631/1‑101 (MQ=255)
                                                                 cGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                <  1:116444/101‑1 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                              >  1:3156786/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                              >  1:5899519/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                              >  1:5278953/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                              >  1:5221038/1‑101 (MQ=255)
                                                                    aaTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAgg                             <  1:353122/101‑1 (MQ=255)
                                                                     aTAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCTAGgt                            >  2:3757860/1‑101 (MQ=255)
                                                                      tAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtg                           >  1:2583718/1‑101 (MQ=255)
                                                                      tAAGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtg                           >  1:1276250/1‑101 (MQ=255)
                                                                       aaGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                          >  2:5789811/1‑101 (MQ=255)
                                                                       aaGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                          >  2:6004586/1‑101 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                         <  1:5790113/101‑1 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                         <  2:2076411/101‑1 (MQ=255)
                                                                        aGACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                         >  2:566380/1‑101 (MQ=255)
                                                                         gACGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttc                        >  2:5906706/1‑101 (MQ=255)
                                                                          aCGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttct                       <  2:4353361/101‑1 (MQ=255)
                                                                          aCGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttct                       <  1:2075304/101‑1 (MQ=255)
                                                                          aCGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttct                       <  1:6102942/101‑1 (MQ=255)
                                                                           cGTCAAAAGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttctt                      <  2:1710454/101‑1 (MQ=255)
                                                                                aaaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCt                 >  2:2361141/1‑101 (MQ=255)
                                                                                 aaGGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCtt                >  1:817293/1‑101 (MQ=255)
                                                                                     ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg            <  1:2656961/101‑1 (MQ=255)
                                                                                     ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg            >  2:1502911/1‑101 (MQ=255)
                                                                                       ggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGCGTTCTTCATCTTTCTGCa          >  1:682025/1‑101 (MQ=255)
                                                                                       cgatcttgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGc           <  2:3072148/93‑1 (MQ=255)
                                                                                         ctctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaaa        <  1:5447323/101‑1 (MQ=255)
                                                                                          tctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAg       >  2:6854175/1‑101 (MQ=255)
                                                                                             tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCagatcg     >  1:3072261/3‑96 (MQ=255)
                                                                                              gcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAcc    >  1:6527090/2‑101 (MQ=255)
                                                                                              gcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAcc    <  1:5120706/100‑1 (MQ=255)
                                                                                                                                                                                                     
GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGC  >  NC_014034/2044055‑2044251

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 40 ≤  ATCG