BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,047,090	+C	100%	coding (20/879 nt)	potH1 ←	polyamine ABC transporter permease PotH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,047,090	1	.→C	100.0%	86.7	359	coding (20/879 nt)	potH1	polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand): new base (140/189): ref base (23/7): total (163/196)
Fisher's exact test strand distribution p-value = 4.13e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.67e-02

Only 100 of 378 total aligned reads displayed.


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                               >  2:6618682/1‑101 (MQ=255)


  acgaTGGTCACCAGCGGGGCCGCCAGCACCAGAACGGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.ccg                                                                                             <  2:1611995/101‑3 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           >  1:4683291/1‑100 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                           >  1:3925635/1‑100 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggt                                                                                          >  1:3743241/1‑99 (MQ=255)


      tGGTCCCCAGCGGGCCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggtt                                                                                         <  2:6392359/101‑4 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGGACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                        >  2:1402988/1‑97 (MQ=255)


         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgga                                                                                      <  2:321121/101‑7 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     >  1:3689209/1‑94 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                     <  1:256932/101‑8 (MQ=255)


           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgggga                                                                                    >  1:790462/1‑93 (MQ=255)


            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGAt                                                                                    >  1:6429398/1‑101 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                  >  1:5155477/1‑101 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                  >  2:1014000/1‑101 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                  >  2:1317185/1‑101 (MQ=255)


               gCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGcc                                                                                 <  2:3861743/101‑1 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                <  1:6602075/101‑1 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                <  1:1651223/101‑1 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                >  2:6762361/1‑101 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                >  2:5940231/1‑101 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                <  2:5450994/101‑1 (MQ=255)


                cGGGCCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                                <  2:3276828/101‑1 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGAc                                                                              >  1:4937064/1‑101 (MQ=255)


                   ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACa                                                                             <  2:4679839/101‑1 (MQ=255)


                     ccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATa                                                                           <  2:5512932/101‑1 (MQ=255)


                      cgccAGCAACAGACCTGCAACGATCAGCGTTGGGCTGCCCATGAGCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTCGAGATGCCGACCTAc                                                                          <  1:5624125/101‑1 (MQ=255)


                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAct                                                                         >  2:2478498/1‑101 (MQ=255)


                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAct                                                                         <  2:1860423/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                        >  2:1971104/1‑101 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                       >  2:4597953/1‑101 (MQ=255)


                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                     <  2:1761413/101‑1 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                    >  2:3114585/1‑101 (MQ=255)


                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                                    <  1:310210/101‑1 (MQ=255)


                             taCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                   <  1:1513768/100‑1 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               <  1:4313968/101‑1 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                               <  2:1465448/101‑1 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGACGCCGACATACTCTATTct.cc                                                               <  1:3169997/101‑1 (MQ=255)


                                  accTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                             <  2:2144415/99‑3 (MQ=255)


                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                            <  1:2900859/101‑4 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                           <  1:4074500/101‑5 (MQ=255)


                                     tGCATAGATCAGCGTTGGCCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctcag                                                          <  2:2923327/101‑6 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          <  1:2055767/101‑1 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          <  2:4499891/101‑1 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          >  1:327930/1‑101 (MQ=255)


                                       cATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg                                                         <  2:2063514/101‑1 (MQ=255)


                                        aTAGACCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATCCTCTATTCTCCCTCAgcgc                                                        <  2:2991242/101‑1 (MQ=255)


                                         tAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcg                                                       >  2:5126152/1‑100 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgt                                                      <  1:2364573/101‑3 (MQ=255)


                                           gATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACACTATTCTCCCTCAgcgcgtc                                                     >  1:4375521/1‑98 (MQ=255)


                                            aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtct                                                    >  2:625124/1‑97 (MQ=255)


                                            aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtct                                                    >  2:4027604/1‑97 (MQ=255)


                                             tCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   >  2:718198/1‑96 (MQ=255)


                                             tCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  1:6697984/101‑6 (MQ=255)


                                              ccgtgTTGGGGTGGCCGTGAACCAGCCTCTGGCCGCCCCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  2:6901793/97‑1 (MQ=255)


                                              cAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  2:4120824/101‑1 (MQ=255)


                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  1:1408452/101‑1 (MQ=255)


                                               aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.g                                                >  2:1221434/1‑101 (MQ=255)


                                               aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.g                                                >  1:1888572/1‑101 (MQ=255)


                                                  gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                             >  1:6307340/1‑98 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  2:1562136/1‑96 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggagcg                                           >  2:2286369/1‑96 (MQ=255)


                                                     gggCGGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGt                                           <  1:7033994/101‑1 (MQ=255)


                                                       gCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:859117/101‑1 (MQ=255)


                                                       gCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:1932646/101‑1 (MQ=255)


                                                       gCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:5032508/101‑1 (MQ=255)


                                                        cTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTg                                        <  1:112283/101‑1 (MQ=255)


                                                        cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTg                                        >  1:6174066/1‑101 (MQ=255)


                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  2:1730437/1‑101 (MQ=255)


                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  1:3605045/1‑101 (MQ=255)


                                                          gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      >  1:4981288/1‑101 (MQ=255)


                                                            ccATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    <  2:1652241/101‑1 (MQ=255)


                                                             cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                   >  1:4585341/1‑101 (MQ=255)


                                                               tGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                 <  2:2655720/101‑1 (MQ=255)


                                                                gacccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                                <  1:6806966/98‑1 (MQ=255)


                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              >  2:2340486/1‑101 (MQ=255)


                                                                   ccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTg                             <  1:5276770/101‑1 (MQ=255)


                                                                       gcGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                         <  2:341590/100‑1 (MQ=255)


                                                                        cGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        <  1:180483/101‑1 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACGTACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  2:423199/101‑1 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  2:5718775/1‑101 (MQ=255)


                                                                           tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     <  2:4845442/101‑1 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                   <  1:6075417/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  1:1965193/101‑1 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  1:3879268/1‑101 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  1:6515166/1‑101 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                >  1:3461962/1‑101 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              <  2:5344428/101‑1 (MQ=255)


                                                                                   tCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag             <  2:3560169/101‑1 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            >  2:5311219/1‑101 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            >  2:6977492/1‑101 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga            <  1:1023887/101‑1 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCTCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          <  1:5342904/101‑1 (MQ=255)


                                                                                        gcgcgcCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        >  2:2752249/1‑101 (MQ=255)


                                                                                        gcgcgcCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa        <  1:2949779/101‑1 (MQ=255)


                                                                                         cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:4130475/96‑1 (MQ=255)


                                                                                          gcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      <  2:4726460/97‑1 (MQ=255)


                                                                                            gcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggg    >  1:4729491/3‑101 (MQ=255)


                                                                                              ccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:5918136/101‑1 (MQ=255)


                                                                                              ccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:6994220/101‑1 (MQ=255)


                                                                                              ccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:6873009/101‑1 (MQ=255)


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:


ATCG

< 3 ≤


ATCG

< 21 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG