Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,833 +A 83.1% intergenic (‑599/‑303) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8331.→A83.1% 137.1 ‑1.4 ‑0.9254intergenic (‑599/‑303)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (110/101):  ref base (27/16):  total (137/117)
Fisher's exact test strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.22e-01

Only 100 of 271 total aligned reads displayed.

GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913
                                                                                                        |                                                                                   
gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgc                                                                                      >  2:1336603/1‑101 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                     >  2:2865171/1‑100 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                     >  2:3101394/1‑100 (MQ=255)
  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                    >  2:4078434/1‑99 (MQ=255)
    tGTTTACCGCCCATTAGCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                 <  2:6979467/101‑2 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                 >  1:451751/1‑100 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                 >  2:707244/1‑100 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                 <  2:4506342/101‑2 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGca                                                                                 <  1:5404943/101‑2 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  1:2420459/101‑5 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              >  2:807310/1‑97 (MQ=255)
        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                             >  2:431747/1‑96 (MQ=255)
        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                             >  1:1536499/1‑96 (MQ=255)
         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGACGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgt                                                                            >  2:3559038/1‑95 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           >  1:6968874/1‑94 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           >  1:4342288/1‑94 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  1:2737644/1‑93 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         >  2:3423709/1‑92 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         >  1:2075904/1‑92 (MQ=255)
             cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttcc                                                                        >  2:1873370/1‑91 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGc                                                                   >  2:1164463/1‑101 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGc                                                                   <  1:3834869/101‑1 (MQ=255)
                      ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCa                                                                  >  1:703780/1‑101 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  2:1046571/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  1:3494541/101‑1 (MQ=255)
                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATc                                                                >  1:55458/1‑101 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                               <  2:5968914/101‑1 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                             >  2:3896350/1‑99 (MQ=255)
                           tccGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                             >  2:5003126/3‑99 (MQ=255)
                            tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            >  2:4650544/1‑101 (MQ=255)
                             cGGTGCGCGGACTTCTGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGG.TTCCGAACCGGCATCATTCt                                                           >  1:6849718/1‑101 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:4556369/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          >  1:6680575/1‑101 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         <  2:3315535/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         <  2:2115182/101‑1 (MQ=255)
                                  cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  1:3350733/101‑1 (MQ=255)
                                    cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  2:5464071/101‑1 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  2:1220521/101‑1 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:4784942/101‑2 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:3892644/1‑99 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATACTGAGCCGCAAGC.GTTTCCGAACCGGCACCATTCTTGG.ccccccgcc                                              >  1:2289632/1‑97 (MQ=255)
                                           cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                             >  1:5707677/1‑101 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCNAACCGGCATCATTCTTGGCCCCCCGCCggg                                            >  1:1830671/1‑101 (MQ=255)
                                             gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                           >  1:1446727/1‑101 (MQ=255)
                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAGTCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  1:296172/101‑1 (MQ=255)
                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          >  1:6701534/1‑101 (MQ=255)
                                               gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACa                                         <  2:5780601/101‑1 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        >  1:3101699/1‑101 (MQ=255)
                                                  gcgtcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  2:7034543/101‑1 (MQ=255)
                                                   cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATcc                                     >  2:3355835/1‑100 (MQ=255)
                                                   cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     >  2:5293833/1‑101 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  1:4361903/1‑101 (MQ=255)
                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:2557015/101‑1 (MQ=255)
                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:2353243/101‑1 (MQ=255)
                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:4267528/101‑1 (MQ=255)
                                                     gcggGGCTCTTGCGATCCTGCCTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   <  2:3270200/101‑1 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGCCAATCACgg                                  >  2:3783305/1‑101 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  1:5333443/1‑101 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 <  1:2538871/101‑1 (MQ=255)
                                                        gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  1:2169722/101‑1 (MQ=255)
                                                        gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                >  1:951935/1‑101 (MQ=255)
                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  1:5286064/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgc                              <  1:807334/101‑1 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgc                              <  1:788446/101‑1 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  2:3636696/1‑101 (MQ=255)
                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          >  1:1150520/1‑101 (MQ=255)
                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          >  2:4577346/1‑101 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:4043376/1‑101 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:1278584/101‑1 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  2:744068/101‑1 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:6752134/1‑101 (MQ=255)
                                                                  gATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  1:472671/1‑101 (MQ=255)
                                                                   aTCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     >  2:4722271/1‑101 (MQ=255)
                                                                     ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:502833/101‑1 (MQ=255)
                                                                     ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  1:6481669/1‑101 (MQ=255)
                                                                        gCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  2:1682982/1‑101 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  1:2798817/101‑1 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:1569043/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:5876851/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:1053895/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  1:4057267/1‑101 (MQ=255)
                                                                           ttccgggATTGGAGACAATGCCGCGCCGCAAGC.GTTTCCGACGCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:5163703/96‑1 (MQ=255)
                                                                            ttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  2:496968/101‑1 (MQ=255)
                                                                            ttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  1:6102767/1‑101 (MQ=255)
                                                                            ttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGCGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  1:3701888/101‑1 (MQ=255)
                                                                              ccGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  1:441431/1‑101 (MQ=255)
                                                                              ccGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          <  2:2645881/101‑1 (MQ=255)
                                                                              ccGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          <  2:6680233/101‑1 (MQ=255)
                                                                                ggATTGGCGACATTCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:6285415/1‑101 (MQ=255)
                                                                                ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        <  2:6285092/101‑1 (MQ=255)
                                                                                ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:5651655/1‑101 (MQ=255)
                                                                                ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        <  1:1683043/101‑1 (MQ=255)
                                                                                 gATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  1:896960/1‑101 (MQ=255)
                                                                                 gATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:6029468/101‑1 (MQ=255)
                                                                                  aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  2:6513017/1‑101 (MQ=255)
                                                                                  aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:3805292/101‑1 (MQ=255)
                                                                                   ttGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:1873442/101‑1 (MQ=255)
                                                                                    tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:1632572/101‑1 (MQ=255)
                                                                                     ggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  2:6432814/101‑1 (MQ=255)
                                                                                     ggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  2:2413804/101‑1 (MQ=255)
                                                                                                        |                                                                                   
GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG