Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,098,581 +C 1.6% coding (48/1491 nt) RCAP_rcc01939 → polysaccharide biosynthesis protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,098,5811.→C 1.6% 4.5 ‑3.0 ‑0.4251coding (48/1491 nt)RCAP_rcc01939polysaccharide biosynthesis protein family
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (115/132):  total (119/132)
Fisher's exact test strand distribution p-value = 4.92e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.77e-01

Only 100 of 304 total aligned reads displayed.

TGCTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCC..GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCA  >  NC_014034/2098481‑2098678
                                                                                                      |                                                                                                   
    ttCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGG.cccc                                                                                                 >  2:2346453/1‑101 (MQ=255)
     tCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCC..g                                                                                              <  2:3896699/101‑1 (MQ=255)
       gAAGGCGGTCGGGCCCCTTCCCGCCCCCCGCCGAGTATAGGGGGCAGTGGCAGCCCGTGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCC..ggc                                                                                            <  2:3258571/101‑3 (MQ=255)
        aaGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGGGGCGGC.GGT.CCCC..gggc                                                                                           >  2:6958490/1‑98 (MQ=255)
          ggCGGTCTGGCGCCTTCACGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCC..ggctcc                                                                                         <  1:4987907/101‑6 (MQ=255)
             ggTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGcc                                                                                       >  1:4250600/1‑101 (MQ=255)
              gTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGG.CCCCG.GCCCCGccc                                                                                      >  2:1654007/1‑101 (MQ=255)
               tCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCg                                                                                     <  2:179966/101‑1 (MQ=255)
                cTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCgg                                                                                    >  2:6900530/1‑101 (MQ=255)
                    cgcCTTCACGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCa                                                                                <  2:487917/101‑1 (MQ=255)
                    cgcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCa                                                                                <  2:4453286/101‑1 (MQ=255)
                     gcCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAg                                                                               <  1:1937529/101‑1 (MQ=255)
                       cTTCACGGCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGcc                                                                             <  2:4033560/101‑1 (MQ=255)
                          cACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCCGCcgcg                                                                          >  1:1723486/1‑101 (MQ=255)
                            cGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGG.CCCCG.GCTCCGCCCGGGCCAGCCCCGGa                                                                        >  1:4616783/1‑101 (MQ=255)
                             ggCCCCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAc                                                                       <  1:1904167/101‑1 (MQ=255)
                             ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCCGCCGCGGAc                                                                       >  1:132289/1‑101 (MQ=255)
                             ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAc                                                                       <  2:889359/101‑1 (MQ=255)
                             ggCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAc                                                                       <  2:5329550/101‑1 (MQ=255)
                              gCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcg                                                                      <  1:1677096/101‑1 (MQ=255)
                               cccccGCACAGTATGGGGGCCAGTGGCACCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcgg                                                                     <  2:4251730/99‑1 (MQ=255)
                               cACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcgg                                                                     >  2:590399/1‑101 (MQ=255)
                                  ccGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGG.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgc                                                                  >  1:5270232/1‑101 (MQ=255)
                                   cGCACATTAGAGGGGACAGTGGCAGCCCAGGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcg                                                                 <  2:2301765/101‑1 (MQ=255)
                                     cacaGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcggg                                                               >  1:296142/1‑100 (MQ=255)
                                     cacaGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcggc                                                               >  1:3647570/1‑101 (MQ=255)
                                       caGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcggggc                                                             >  1:5155232/1‑101 (MQ=255)
                                        ggTATGGGGGCCAGCGGCCCCCCACGACCCGCTATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcggcgcg                                                            <  1:681355/100‑1 (MQ=255)
                                          tataGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGTCCCCC..GCTCCGCCCGGGCCAGCCGCGGGcggcgcggcgcggg                                                          >  1:247788/1‑100 (MQ=255)
                                          tataGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGAcggcgcggcgcggc                                                          >  1:7113303/1‑101 (MQ=255)
                                           ataGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCCGCCGCGGCcggcgcggcgcggcg                                                         >  1:4092019/1‑101 (MQ=255)
                                             aGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGGcggcgcggcgcggccgg                                                       >  2:2027190/1‑98 (MQ=255)
                                             aGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCgcggcggg                                                       >  1:2545647/1‑100 (MQ=255)
                                               gggACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCgcggcggcgc                                                     >  2:782404/1‑100 (MQ=255)
                                                ggACAGTGGCAGCCCATGCCCCGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGAt                                                    <  1:6260518/101‑1 (MQ=255)
                                                ggACAGTGGCACCCCATGCCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGAt                                                    <  2:4200440/101‑1 (MQ=255)
                                                 gACAGGGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATc                                                   <  1:6077386/101‑1 (MQ=255)
                                                  gaggTGCCACCCCATTACCAGCGATTCGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCt                                                  <  2:3334192/98‑1 (MQ=255)
                                                  aCAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGTCt                                                  >  1:4901289/1‑101 (MQ=255)
                                                   cAGTGGCACCCCATGCCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCtt                                                 <  1:1603730/101‑1 (MQ=255)
                                                    aGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGG.CCCCG.GCTCCGCCCCGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGCCTTc                                                >  2:1342205/1‑101 (MQ=255)
                                                    aGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCCCCCCGGCGGC.GGG.CCCCCGGCCCCCCCCGGGCC.GCCGCGGGCGGCGCGGGGGGGCGGCGGTCTTc                                                >  1:5347452/1‑101 (MQ=255)
                                                    aGTGCCACCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTc                                                <  1:2788044/101‑1 (MQ=255)
                                                       ggCAGCCCATGCCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGTGGCGCGGCGGCGATCTTCGcc                                             <  2:3747238/101‑1 (MQ=255)
                                                       ggCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGCGGGC.CCCC..GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCAGCGATccacgac                                             >  2:4520372/1‑95 (MQ=255)
                                                       ggCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGcc                                             >  2:2875875/1‑101 (MQ=255)
                                                       ggCACCCCATGCCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGcc                                             <  1:6604900/101‑1 (MQ=255)
                                                        gCAGCCCATGACCAGCGATTTCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCt                                            <  1:2346548/101‑1 (MQ=255)
                                                         cAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGGTCTTCGCCtt                                           >  2:5069653/1‑101 (MQ=255)
                                                         cAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCtt                                           <  1:893733/101‑1 (MQ=255)
                                                          aGCCCATTCCCGGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCttt                                          <  2:6018651/101‑1 (MQ=255)
                                                             ccaggcccggCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                       <  2:5210570/92‑1 (MQ=255)
                                                             ccATGCCCAGCGTTTGCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                       <  1:4150379/101‑1 (MQ=255)
                                                             ccATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTc                                       >  2:6751588/1‑101 (MQ=255)
                                                             caaTGACCGGCGATGACCCCCCCCCCGCCGCGGCGGC.GGC.CCCCG.GCTCCGCCCGGGCTAGCGGCGGACGGCGCGGCTCGGCGGCGATCTTCGCCTTTGTc                                       <  1:5782749/99‑1 (MQ=255)
                                                              catgccaggCGATTGCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      <  2:6502078/93‑1 (MQ=255)
                                                              cATGCCCAGCGTTTTCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      <  2:5594973/101‑1 (MQ=255)
                                                              cATGCCCAGCGTTTTCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      <  1:5913385/101‑1 (MQ=255)
                                                              cATGCCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      <  2:5801319/101‑1 (MQ=255)
                                                              cATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      >  1:4857836/1‑101 (MQ=255)
                                                              cATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcg                                      >  1:1807929/1‑101 (MQ=255)
                                                              cATGACCAGCGATTTGCCCCCCCCCCCCGCGGCGGC.GGG.CCCCG.GCTCCCCCCGGGCCAGCCGCGGACGGCGCCGCGCGGCGGGGGTCTTCGCCctttttc                                      >  2:1116823/1‑97 (MQ=255)
                                                               aTGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcgc                                     >  1:2239882/1‑101 (MQ=255)
                                                                 gACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcgcgc                                   >  1:563625/1‑101 (MQ=255)
                                                                  aCCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTcgcgcg                                  >  1:3348104/1‑101 (MQ=255)
                                                                   ccAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCgg                                 >  2:2486709/1‑101 (MQ=255)
                                                                    cAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGGGGCGGTCTTCGCCTTTGTCGCGCggg                                >  1:5976679/1‑101 (MQ=255)
                                                                      gcctttcccccccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCg                              <  2:6462467/93‑1 (MQ=255)
                                                                       cGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCgc                             >  1:3569370/1‑100 (MQ=255)
                                                                        gttttGCCCCCCCCGGCCGCGGCGTG.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGAc                            <  1:4317360/99‑1 (MQ=255)
                                                                        gATTCGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGAc                            <  2:3667239/101‑1 (MQ=255)
                                                                         ttttCCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                           <  1:3965983/100‑1 (MQ=255)
                                                                         tttcgCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACg                           <  2:5874580/97‑1 (MQ=255)
                                                                          tttGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCCTTGTCGCGCCGGCGACgc                          >  2:2673844/1‑101 (MQ=255)
                                                                          tttGCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCCCCTTTGTCGCGCGGGCGCCgc                          >  1:7020627/1‑101 (MQ=255)
                                                                           ttccccccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgca                         <  2:4616544/98‑1 (MQ=255)
                                                                           tgccccccccccGCCGCGGCGGC.GGT.CCCCG.GCCCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgca                         <  2:7112929/98‑1 (MQ=255)
                                                                             gCCCCCCCCCGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcagc                       >  1:4508150/1‑101 (MQ=255)
                                                                              cccccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACgcagca                      <  2:4975521/101‑1 (MQ=255)
                                                                               ccccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGCCGcagcag                     >  1:6881344/1‑101 (MQ=255)
                                                                               ccccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGcagcag                     <  1:3781432/101‑1 (MQ=255)
                                                                                cccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCcgcagc                    >  1:3186837/1‑101 (MQ=255)
                                                                                cccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCagcagc                    <  1:2845500/101‑1 (MQ=255)
                                                                                 ccccccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCt                   <  1:1145800/101‑1 (MQ=255)
                                                                                    cccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTc                >  2:6572415/1‑101 (MQ=255)
                                                                                    cccGCCGCGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTc                <  1:4423284/101‑1 (MQ=255)
                                                                                       gccgcGGCGGC.GGC.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGAc             <  1:2717153/101‑1 (MQ=255)
                                                                                        ccgcGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGTCGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGAcc            >  2:1207337/1‑101 (MQ=255)
                                                                                        ccgcGGCGGC.GGT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGAcc            <  1:4719119/101‑1 (MQ=255)
                                                                                           cggcggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCttt         <  2:4702947/101‑1 (MQ=255)
                                                                                           cggcggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCttt         <  2:2757426/101‑1 (MQ=255)
                                                                                             gcggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGt       <  2:3026479/101‑1 (MQ=255)
                                                                                             gcggc.ggC.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGt       <  1:5498124/101‑1 (MQ=255)
                                                                                              gggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtc      <  1:2496759/100‑1 (MQ=255)
                                                                                               ggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtca     >  1:3848934/1‑101 (MQ=255)
                                                                                               ggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtca     >  2:2451199/1‑101 (MQ=255)
                                                                                               ggc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtca     <  2:4117432/101‑1 (MQ=255)
                                                                                                gc.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcat    <  2:6250972/101‑1 (MQ=255)
                                                                                                 c.ggT.CCCCG.GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatc   <  1:1089781/101‑1 (MQ=255)
                                                                                                   ggT.CCCCG.GCTCCGCCCGGGGCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGtcatca  >  1:6380237/1‑101 (MQ=255)
                                                                                                      |                                                                                                   
TGCTTTCGAAGGCGGTCTGGCGCCTTCACGGCACCCGCACAGTATAGGGGACAGTGGCAGCCCATGACCAGCGATTTGCCCCCCCCCGCCGCGGCGGC.GGT.CCCC..GCTCCGCCCGGGCCAGCCGCGGACGGCGCGGCGCGGCGGCGATCTTCGCCTTTGTCGCGCGGGCGACGCAGCAGCTTTCGACCTTTGTCATCA  >  NC_014034/2098481‑2098678

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG