BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,135,137	+G	98.6%	intergenic (‑131/+80)	RCAP_rcc01974 ← / ← RCAP_rcc01975	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,135,137	1	.→G	98.6%	5.9 ‑1.0 ‑0.7	359	intergenic (‑131/+80)	RCAP_rcc01974/RCAP_rcc01975	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (176/178): ref base (4/1): total (180/179)
Fisher's exact test strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.17e-01

Only 100 of 406 total aligned reads displayed.


AATGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GGG.CCAAAGTTCAAAG.G.C.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCG  >  NC_014034/2135037‑2135231


aaggTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGa                                                                                                      <  2:1597096/98‑1 (MQ=255)


aaTGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGa                                                                                                      >  1:6024838/1‑101 (MQ=255)


aaTGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGa                                                                                                      >  1:4256386/1‑101 (MQ=255)


 aTGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.g                                                                                                    >  1:3489033/1‑101 (MQ=255)


  tGTTGCGATAAGCGCAATTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg                                                                                                   <  2:6471705/101‑1 (MQ=255)


  tGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.gg                                                                                                   >  2:5131951/1‑101 (MQ=255)


  tGTGGCGATAAGCGCAACTTCTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTCA.gg                                                                                                   <  1:6850926/101‑1 (MQ=255)


   gTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggg                                                                                                  >  2:3975516/1‑101 (MQ=255)


   gTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggg                                                                                                  >  2:2058162/1‑101 (MQ=255)


   gTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggg                                                                                                  >  1:922349/1‑101 (MQ=255)


     tGCGATAAGCGCAATTTGTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggggc                                                                                                <  1:2025429/101‑3 (MQ=255)


     tGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggggc                                                                                                >  2:2506834/1‑99 (MQ=255)


       cGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.ggggcca                                                                                              >  1:3679465/1‑97 (MQ=255)


         aTAAGCGCAATTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCaaa                                                                                            <  2:5371145/101‑1 (MQ=255)


         aTAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCaaa                                                                                            >  2:484600/1‑101 (MQ=255)


         aTAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCaaa                                                                                            >  2:1041578/1‑101 (MQ=255)


          tAAGCGCAACTTTTGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAg                                                                                           <  1:3798880/101‑1 (MQ=255)


           aaGCGCAATTTATGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGt                                                                                          <  1:3372101/101‑1 (MQ=255)


            aGCGCAACTTAGGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGtt                                                                                         <  2:2442714/101‑1 (MQ=255)


             gcgcAATTTAGGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTc                                                                                        <  1:5682405/101‑1 (MQ=255)


             gcgcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTc                                                                                        >  1:5547265/1‑101 (MQ=255)


             gcgaattttATGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGTGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTc                                                                                        <  2:303129/94‑1 (MQ=255)


               gcAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCaa                                                                                      <  1:372203/101‑1 (MQ=255)


                cAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCaaa                                                                                     >  2:6027765/1‑101 (MQ=255)


                 aatttATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAg                                                                                    <  2:214330/98‑1 (MQ=255)


                  aCTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAg.g                                                                                  >  1:3553769/1‑101 (MQ=255)


                   cTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGGTCAAAG.G.c                                                                                >  2:621241/1‑101 (MQ=255)


                    ttATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c                                                                              >  1:17991/1‑101 (MQ=255)


                      aTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.cc                                                                           >  2:7117525/1‑100 (MQ=255)


                      aTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.cc                                                                           >  1:2114473/1‑100 (MQ=255)


                      aTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.cc                                                                           <  2:4187588/101‑2 (MQ=255)


                        ggTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.ccgc                                                                         <  2:4279837/101‑4 (MQ=255)


                         gTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.ccgct                                                                        <  2:5540308/101‑5 (MQ=255)


                          tCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.ccgctt                                                                       >  1:2290413/1‑96 (MQ=255)


                           cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCttt                                                                       >  2:7009649/1‑101 (MQ=255)


                           cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.c.ccgattt                                                                      >  2:2638074/1‑95 (MQ=255)


                           cAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGCGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCttt                                                                       <  1:3644385/101‑1 (MQ=255)


                            aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTc                                                                      <  1:3210403/101‑1 (MQ=255)


                              gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GAC.C.CGCTTTCCa                                                                    >  1:1046101/1‑101 (MQ=255)


                                cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGc                                                                  >  1:4614118/1‑101 (MQ=255)


                                cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGc                                                                  <  1:1012090/101‑1 (MQ=255)


                                  ttAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCgg                                                                >  2:9275/1‑101 (MQ=255)


                                     aaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGc                                                             >  2:4642718/1‑101 (MQ=255)


                                      aTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGcc                                                            >  2:167187/1‑101 (MQ=255)


                                         gCGTTTATCGCATCTGTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAg                                                         <  2:19773/101‑1 (MQ=255)


                                           gTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGAGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.C.CGCGGTTTCCACCGGGGCCAAggg                                                       >  2:3281823/1‑101 (MQ=255)


                                            tttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCCAAGTTCAACG.G.CAC.CGCTTTCCATCGGGGCCAAgggg                                                      >  2:4392810/1‑101 (MQ=255)


                                            tttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAgggg                                                      >  2:5897433/1‑101 (MQ=255)


                                             ttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGc                                                     >  1:6098848/1‑101 (MQ=255)


                                              tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcg                                                    >  2:617523/1‑101 (MQ=255)


                                              tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcg                                                    <  1:1358084/101‑1 (MQ=255)


                                                tCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcgac                                                  <  2:6863106/101‑1 (MQ=255)


                                                tCGCAACGTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcgac                                                  <  1:3280322/101‑1 (MQ=255)


                                                 cGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcgacg                                                 >  1:1420813/1‑101 (MQ=255)


                                                  gCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGcgacga                                                <  2:2234857/101‑1 (MQ=255)


                                                   cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCgacgac                                               <  1:4839166/101‑1 (MQ=255)


                                                   cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCgacgac                                               <  1:6460807/101‑1 (MQ=255)


                                                       tttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTg                                           >  2:812112/1‑101 (MQ=255)


                                                        ttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGa                                          <  1:1898628/101‑1 (MQ=255)


                                                          gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                        >  2:5210590/1‑101 (MQ=255)


                                                           cTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAgg                                       <  2:1410003/101‑1 (MQ=255)


                                                              ccGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaa                                    <  2:3716330/101‑1 (MQ=255)


                                                              ccGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGCGAATGCaa                                    >  1:6911759/1‑101 (MQ=255)


                                                              ccGCGTCGATTCGTGAAAGCTTAGACACGCGGGGTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaa                                    <  2:2306721/101‑1 (MQ=255)


                                                               cgcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaaa                                   <  2:4251406/101‑1 (MQ=255)


                                                                gcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAg                                  <  1:7125087/101‑1 (MQ=255)


                                                                 cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                 <  1:29732/101‑1 (MQ=255)


                                                                    cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                              >  1:5932116/1‑101 (MQ=255)


                                                                      aTTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTg                            >  2:5043429/1‑101 (MQ=255)


                                                                       ttCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                           >  2:3529264/1‑101 (MQ=255)


                                                                       ttCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                           <  2:1893368/101‑1 (MQ=255)


                                                                        tCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTc                          <  1:5334294/101‑1 (MQ=255)


                                                                         cGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCg                         >  1:3824782/1‑101 (MQ=255)


                                                                         cGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCg                         >  1:2871787/1‑101 (MQ=255)


                                                                          gTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGc                        >  1:119983/1‑101 (MQ=255)


                                                                           tGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCt                       <  2:4104523/101‑1 (MQ=255)


                                                                            ggAAGCTTCGACACGCGGTCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                      <  2:5691933/101‑1 (MQ=255)


                                                                            ggAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                      >  1:2491156/1‑101 (MQ=255)


                                                                            ggAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                      >  1:4333446/1‑101 (MQ=255)


                                                                             gAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                     >  2:3199821/1‑101 (MQ=255)


                                                                             gAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTAc                     <  1:3255094/101‑1 (MQ=255)


                                                                              aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                    <  2:6682615/101‑1 (MQ=255)


                                                                              aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                    <  1:3160367/101‑1 (MQ=255)


                                                                              aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                    >  1:4434643/1‑101 (MQ=255)


                                                                                gCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGa                  <  1:2358382/101‑1 (MQ=255)


                                                                                gCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGa                  >  2:1364966/1‑101 (MQ=255)


                                                                                  ttCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                <  1:6616808/101‑1 (MQ=255)


                                                                                  ttCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                >  1:6366575/1‑101 (MQ=255)


                                                                                   tCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCt               <  2:3824619/101‑1 (MQ=255)


                                                                                     gACGCGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg             <  1:6259797/101‑1 (MQ=255)


                                                                                     gACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg             <  1:3905928/101‑1 (MQ=255)


                                                                                         cgcgGGCTTTGA.GGGTCCTAAGTTCAAAGAC.C.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGatat         <  2:4902779/101‑1 (MQ=255)


                                                                                          gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata        <  1:4911240/101‑1 (MQ=255)


                                                                                          gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata        >  1:5144205/1‑101 (MQ=255)


                                                                                          gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata        <  1:6809647/101‑1 (MQ=255)


                                                                                            gggCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt      <  2:5954782/101‑1 (MQ=255)


                                                                                            gggCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt      >  2:3627287/1‑101 (MQ=255)


                                                                                              gCTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAATGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc    <  2:6911405/101‑1 (MQ=255)


                                                                                               cTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc   <  1:2624225/101‑1 (MQ=255)


                                                                                               cTTTGAGGGG.CCAAAGTTCAAAG.GCC.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc   >  2:1219603/1‑101 (MQ=255)


AATGTTGCGATAAGCGCAACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GGG.CCAAAGTTCAAAG.G.C.C.CGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCG  >  NC_014034/2135037‑2135231

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG