Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,135,154 +C 98.8% intergenic (‑148/+63) RCAP_rcc01974 ← / ← RCAP_rcc01975 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,135,1541.→C98.8% 3.0 ‑1.1 ‑1.9346intergenic (‑148/+63)RCAP_rcc01974/RCAP_rcc01975hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (155/187):  ref base (3/1):  total (158/188)
Fisher's exact test strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.23e-02

Only 100 of 392 total aligned reads displayed.

ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GGG.CCAAAGTTCAAAG.G.C.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249
                                                                                                       |                                                                                                
 cTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c                                                                                                 >  2:5361863/1‑101 (MQ=255)
    aTGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.ccc                                                                                             >  2:5834107/1‑100 (MQ=255)
      ggTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.cccgc                                                                                           >  2:5984517/1‑98 (MQ=255)
      ggTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.cccgc                                                                                           <  2:4279837/101‑4 (MQ=255)
       gTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.cccgct                                                                                          >  2:6524486/1‑97 (MQ=255)
        tCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.c.cccgctt                                                                                         <  2:4514992/101‑6 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCa                                                                                      >  2:1430907/1‑101 (MQ=255)
             ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAg                                                                                     >  2:799463/1‑101 (MQ=255)
              cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGc                                                                                    >  1:4614118/1‑101 (MQ=255)
              cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGc                                                                                    >  2:2131766/1‑101 (MQ=255)
              cATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGc                                                                                    <  1:1012090/101‑1 (MQ=255)
                 tAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCggg                                                                                 <  1:2318403/101‑1 (MQ=255)
                 tAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.G.CGCCGCTTTCCAGCggg                                                                                 >  2:6185351/1‑101 (MQ=255)
                  aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCgggg                                                                                >  1:326556/1‑101 (MQ=255)
                    aTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGcc                                                                              >  1:6098941/1‑101 (MQ=255)
                         gTTTATCGCTACTTTGCTGCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAggg                                                                         <  2:1655623/101‑1 (MQ=255)
                         gTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAggg                                                                         >  2:4964476/1‑101 (MQ=255)
                            tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGcg                                                                      <  1:1358084/101‑1 (MQ=255)
                              tCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGcgac                                                                    <  2:4245361/101‑1 (MQ=255)
                               cGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGcgacg                                                                   <  2:3335435/101‑1 (MQ=255)
                                 cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCgacgac                                                                 <  1:4839166/101‑1 (MQ=255)
                                  aaCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGtctacg                                                                >  1:2241250/1‑101 (MQ=255)
                                  aaCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGacgacg                                                                >  1:6471330/1‑101 (MQ=255)
                                    cTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTCAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGt                                                              >  2:6076861/1‑101 (MQ=255)
                                     tttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTg                                                             >  2:6990311/1‑101 (MQ=255)
                                         cTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAgg                                                         <  2:1063046/101‑1 (MQ=255)
                                         cTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAgg                                                         <  2:1410003/101‑1 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        <  2:592528/101‑1 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        >  1:2418507/1‑101 (MQ=255)
                                           tCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCa                                                       >  2:1247213/1‑101 (MQ=255)
                                            ccGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGCGAATGCaa                                                      >  1:6911759/1‑101 (MQ=255)
                                              gcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAg                                                    <  1:7125087/101‑1 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   >  2:3645444/1‑101 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   <  1:3586732/101‑1 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   >  2:435014/1‑101 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   <  1:29732/101‑1 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGc                                                   <  1:1409723/101‑1 (MQ=255)
                                                  tgATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                <  2:2313516/100‑1 (MQ=255)
                                                  ggATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                <  2:2241155/100‑1 (MQ=255)
                                                  cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                >  1:5932116/1‑101 (MQ=255)
                                                  cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                <  1:1044391/101‑1 (MQ=255)
                                                   gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACt                                               >  1:4095656/1‑101 (MQ=255)
                                                     ttcggtgAAGCTTCGACGCGCGGGCTGTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTGTCCAGGGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGt                                             <  1:3306775/95‑1 (MQ=255)
                                                       cGTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCg                                           >  1:3824782/1‑101 (MQ=255)
                                                        gTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGc                                          <  1:993176/101‑1 (MQ=255)
                                                        gTGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGc                                          >  1:119983/1‑101 (MQ=255)
                                                         tGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCt                                         <  2:4104523/101‑1 (MQ=255)
                                                         tGGAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGAAACTGTCGCt                                         <  1:3738224/101‑1 (MQ=255)
                                                          ggAAGTTTCGACACGCGGGCGTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        <  2:4172236/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        <  1:2058242/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        >  1:4333446/1‑101 (MQ=255)
                                                            aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                                      >  1:4434643/1‑101 (MQ=255)
                                                            aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                                      <  1:3212006/101‑1 (MQ=255)
                                                            aaGCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                                      >  1:6025568/1‑101 (MQ=255)
                                                              gCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGa                                    >  2:1364966/1‑101 (MQ=255)
                                                              gCTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGa                                    <  2:359612/101‑1 (MQ=255)
                                                               cTTCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCCAAGCAACGGTCGCTACTGAt                                   <  1:6383499/101‑1 (MQ=255)
                                                                ttCGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                                  <  1:6616808/101‑1 (MQ=255)
                                                                  cGACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTa                                >  1:2492123/1‑101 (MQ=255)
                                                                   gACGCGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg                               <  1:6259797/101‑1 (MQ=255)
                                                                   gACACGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg                               <  1:3173735/101‑1 (MQ=255)
                                                                    acgcgcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              <  2:7105499/98‑1 (MQ=255)
                                                                    acacGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              >  2:5578692/1‑101 (MQ=255)
                                                                    acacGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              <  1:6799913/101‑1 (MQ=255)
                                                                    acacGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCCAGa                              <  1:5600821/101‑1 (MQ=255)
                                                                     cacGCGGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGat                             <  2:2637751/101‑1 (MQ=255)
                                                                       cgcgGGCTTTGA.GGGTCCTAAGTTCAAAGAC.C.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGatat                           <  2:4902779/101‑1 (MQ=255)
                                                                        gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          <  1:4911240/101‑1 (MQ=255)
                                                                        gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          >  1:3655560/1‑101 (MQ=255)
                                                                        gcgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          <  1:3417521/101‑1 (MQ=255)
                                                                         cgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         <  2:3220365/101‑1 (MQ=255)
                                                                         cgGGCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         <  1:1865518/101‑1 (MQ=255)
                                                                         cgGGCCTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         >  2:923995/1‑101 (MQ=255)
                                                                          gggCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        <  2:1925009/101‑1 (MQ=255)
                                                                          gggATTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        >  1:6837540/1‑101 (MQ=255)
                                                                            gCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc                      <  2:689730/101‑1 (MQ=255)
                                                                            gCTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc                      <  1:3853686/101‑1 (MQ=255)
                                                                             gtttGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc                     <  2:5082409/100‑1 (MQ=255)
                                                                             cTTTGAGGGG.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc                     <  1:2624225/101‑1 (MQ=255)
                                                                               ttgagggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGc                   >  2:2516902/6‑101 (MQ=255)
                                                                                tgagggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGGAAAGCAACTGTCGCTACTGACCTAGATATAATTCCGcc                  >  2:4736453/5‑101 (MQ=255)
                                                                                tgagggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  1:1538351/5‑101 (MQ=255)
                                                                                tgagggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  1:668497/5‑101 (MQ=255)
                                                                                  agggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCaa                >  1:5261212/3‑101 (MQ=255)
                                                                                   gggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAAt               >  2:6719710/2‑101 (MQ=255)
                                                                                   gggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAAt               <  2:6347738/100‑1 (MQ=255)
                                                                                    ggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATg              <  1:5132211/101‑1 (MQ=255)
                                                                                    ggg.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATg              <  1:1987222/101‑1 (MQ=255)
                                                                                      g.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            <  2:383317/101‑1 (MQ=255)
                                                                                      g.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            <  1:3069274/101‑1 (MQ=255)
                                                                                      g.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            <  2:5215873/101‑1 (MQ=255)
                                                                                      g.CCAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGtt            >  2:3057530/1‑101 (MQ=255)
                                                                                        ccAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTg           <  2:1992799/101‑1 (MQ=255)
                                                                                        ccAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTg           <  2:1768076/101‑1 (MQ=255)
                                                                                        ccAAAGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTg           <  1:5362135/101‑1 (MQ=255)
                                                                                            aGTTCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCg       >  2:6208485/1‑101 (MQ=255)
                                                                                              ttCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGa     <  1:812136/101‑1 (MQ=255)
                                                                                               tCAAAG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGac    >  1:2861824/1‑101 (MQ=255)
                                                                                                 aaaG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGacac  >  2:547273/1‑101 (MQ=255)
                                                                                                 aaaG.GCC.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGacac  <  2:2871678/101‑1 (MQ=255)
                                                                                                       |                                                                                                
ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GGG.CCAAAGTTCAAAG.G.C.CCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG