Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,276 +G 100% coding (101/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2761.→G100.0% 917.4 253coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (139/111):  ref base (2/1):  total (141/112)

Only 100 of 295 total aligned reads displayed.

ACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTT..G..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCC  >  NC_014034/2177180‑2177353
                                                                                                       |                                                                                   
acaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..t                                                                                <  1:4745014/101‑1 (MQ=255)
 caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tg                                                                               >  1:6283456/1‑100 (MQ=255)
  aTCGCCATCTATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgt                                                                              <  2:2224564/101‑3 (MQ=255)
  aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgt                                                                              <  1:2522710/101‑3 (MQ=255)
  aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgt                                                                              >  2:3192024/1‑99 (MQ=255)
    cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcg                                                                            >  2:1554143/1‑97 (MQ=255)
    cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcg                                                                            <  1:1322925/101‑5 (MQ=255)
    cGCCACCGACCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcg                                                                            <  1:2236789/101‑5 (MQ=255)
       cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcggtc                                                                         <  2:800904/101‑8 (MQ=255)
       cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcggtc                                                                         <  2:1223290/101‑8 (MQ=255)
         tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..g..g..tgtcggtctt                                                                       <  1:7020991/101‑10 (MQ=255)
          cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.g                                                                       >  2:4545631/1‑101 (MQ=255)
          cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.g                                                                       >  1:725240/1‑101 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.gg                                                                      >  2:4146521/1‑100 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.gg                                                                      >  2:6025653/1‑100 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.gg                                                                      <  2:2757341/101‑2 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.gg                                                                      >  1:102319/1‑100 (MQ=255)
            aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggt                                                                     <  2:357225/101‑3 (MQ=255)
            aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggt                                                                     <  1:4632171/101‑3 (MQ=255)
               ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgat                                                                  >  1:3367193/1‑96 (MQ=255)
               ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgat                                                                  >  2:3039974/1‑96 (MQ=255)
               ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgat                                                                  <  2:5836909/101‑6 (MQ=255)
                tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgatc                                                                 >  2:1767510/1‑95 (MQ=255)
                tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgatc                                                                 <  2:6739463/101‑7 (MQ=255)
                 gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgatca                                                                >  2:728524/1‑94 (MQ=255)
                    cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTt.ggtgatcagac                                                             <  2:5061914/101‑11 (MQ=255)
                      cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGAtcagacag                                                            >  2:3125991/1‑101 (MQ=255)
                      cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGAtcagacag                                                            <  2:3363886/101‑1 (MQ=255)
                      cATAGGTCGAGAGCACCAGCCCCATCTCGGNCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGAtcagacag                                                            <  1:2822373/101‑1 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAgg                                                           >  2:141270/1‑101 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAgg                                                           >  1:3228219/1‑101 (MQ=255)
                        tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGc                                                          <  1:3189169/101‑1 (MQ=255)
                        tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGc                                                          >  2:6141692/1‑101 (MQ=255)
                         aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGcc                                                         >  1:4879223/1‑101 (MQ=255)
                          ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGccc                                                        >  2:5897778/1‑101 (MQ=255)
                          ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGccc                                                        <  2:3898558/101‑1 (MQ=255)
                          ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGccc                                                        >  1:5572367/1‑101 (MQ=255)
                           tctgAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGcccc                                                       <  2:1281417/98‑1 (MQ=255)
                             cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCgg                                                     >  2:6734748/1‑101 (MQ=255)
                              gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGgcccaga                                                    >  1:1281465/1‑98 (MQ=255)
                               agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                   >  1:4472679/4‑101 (MQ=255)
                               agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                   <  1:6882856/98‑1 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                  <  1:6133351/99‑1 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGAACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGG..G..TCGGTCTTGGTGATCAGACAGGCCCCgcgtt                                                  >  2:6928645/3‑97 (MQ=255)
                                 agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                 <  1:2721977/100‑1 (MQ=255)
                                  gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                <  2:4445084/101‑1 (MQ=255)
                                    cccACCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                              <  1:3336668/100‑1 (MQ=255)
                                    aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                              >  2:2419112/1‑101 (MQ=255)
                                     ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                             >  1:989649/1‑101 (MQ=255)
                                      cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                            >  2:6516097/1‑101 (MQ=255)
                                      cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                            >  1:5436595/1‑101 (MQ=255)
                                      cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                            <  1:3727032/101‑1 (MQ=255)
                                       aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                          >  1:5290638/1‑101 (MQ=255)
                                        gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                         <  1:758724/101‑2 (MQ=255)
                                        gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                         <  2:5556216/101‑2 (MQ=255)
                                         ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                        >  1:4998200/1‑99 (MQ=255)
                                          cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                       <  2:5144240/101‑4 (MQ=255)
                                          cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                       <  1:1158659/101‑4 (MQ=255)
                                            cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                     >  2:1136950/1‑96 (MQ=255)
                                                tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                  <  1:3839315/101‑1 (MQ=255)
                                                 cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                 <  1:3128263/101‑1 (MQ=255)
                                                  ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                >  1:4188001/1‑101 (MQ=255)
                                                       ccTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                           <  2:6327953/101‑1 (MQ=255)
                                                       ccTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                           >  2:379502/1‑101 (MQ=255)
                                                        cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                          >  1:166829/1‑101 (MQ=255)
                                                        cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                          >  2:3471048/1‑101 (MQ=255)
                                                           tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                       >  2:4199414/1‑101 (MQ=255)
                                                             ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                     <  2:903212/101‑1 (MQ=255)
                                                             ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                     <  1:6735090/101‑1 (MQ=255)
                                                              cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                    <  1:1945902/101‑1 (MQ=255)
                                                              cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                    <  2:5605554/101‑1 (MQ=255)
                                                               gAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                   >  1:2850857/1‑101 (MQ=255)
                                                               gAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                   <  1:3537886/101‑1 (MQ=255)
                                                                aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                  <  1:5391602/101‑1 (MQ=255)
                                                                aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                  >  2:4336992/1‑101 (MQ=255)
                                                                aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                  >  2:6284777/1‑101 (MQ=255)
                                                                  gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                >  2:2531885/1‑101 (MQ=255)
                                                                  gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGAAAAACGc                >  1:3065350/1‑101 (MQ=255)
                                                                    aCCTTCCTTCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa              <  2:304914/101‑1 (MQ=255)
                                                                     ccttccttCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g            >  2:3416091/1‑101 (MQ=255)
                                                                     ccttccttCATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g            >  2:4713858/1‑101 (MQ=255)
                                                                      cttccttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg           <  2:539091/101‑1 (MQ=255)
                                                                       ttccttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg          <  2:3986841/101‑2 (MQ=255)
                                                                        tccttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc         >  2:5678019/1‑99 (MQ=255)
                                                                         ccttcATCTTTACGAAAAA..GGTGTT..G..GTTTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca        <  1:1047807/101‑4 (MQ=255)
                                                                         ccttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca        <  2:5323030/101‑4 (MQ=255)
                                                                         ccttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca        <  1:5762143/101‑4 (MQ=255)
                                                                           ttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga      >  2:6611590/1‑96 (MQ=255)
                                                                           ttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga      >  1:5479165/1‑96 (MQ=255)
                                                                           ttcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga      >  1:1434676/1‑96 (MQ=255)
                                                                            tcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag      >  2:455211/1‑101 (MQ=255)
                                                                            tcATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag      >  1:4264721/1‑101 (MQ=255)
                                                                             cATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg     <  1:6610170/101‑1 (MQ=255)
                                                                             cATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg     >  1:5914718/1‑101 (MQ=255)
                                                                             cATCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg     >  2:6437668/1‑101 (MQ=255)
                                                                              aTCTTGACG.AAAAATGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc    <  1:6928999/101‑1 (MQ=255)
                                                                              aTCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc    >  1:4019831/1‑101 (MQ=255)
                                                                               tCTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcc   >  1:1997928/1‑101 (MQ=255)
                                                                                cTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc  <  1:2372150/101‑1 (MQ=255)
                                                                                cTTGACG.AAAAAGGG.GTT..GGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc  >  1:1567/1‑101 (MQ=255)
                                                                                                       |                                                                                   
ACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTT..G..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCC  >  NC_014034/2177180‑2177353

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG