Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 1193.0 302coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (174/126):  ref base (1/0):  total (175/127)

Only 100 of 325 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGG  >  NC_014034/2177218‑2177405
                                                                                                       |                                                                                               
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                  >  2:6516097/1‑101 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                  <  1:3727032/101‑1 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                                                >  1:5290638/1‑101 (MQ=255)
  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                                                                               <  2:5556216/101‑2 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                                                            <  2:3041653/101‑5 (MQ=255)
        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                                                          <  1:1034807/101‑1 (MQ=255)
        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCAGCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                                                          >  1:5014790/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                         <  2:830976/101‑1 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                        <  1:3839315/101‑1 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                        <  1:2844406/101‑1 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                      >  2:3118737/1‑101 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                      >  2:2817737/1‑101 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                      >  1:4188001/1‑101 (MQ=255)
             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                                                     >  2:3369807/1‑101 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                  >  1:3372200/1‑101 (MQ=255)
                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                                                >  1:166829/1‑101 (MQ=255)
                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                                               >  2:4546050/1‑101 (MQ=255)
                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                                                                              <  1:4716431/101‑1 (MQ=255)
                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                                                                              >  1:3246352/1‑101 (MQ=255)
                     tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                                             >  2:4199414/1‑101 (MQ=255)
                     tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                                             >  1:7073905/1‑101 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                          <  1:1945902/101‑1 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGcaaa                                                                         >  2:2751491/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGcaaa                                                                         >  2:4295062/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                                                         <  1:3537886/101‑1 (MQ=255)
                            gCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                      >  2:2531885/1‑101 (MQ=255)
                            gCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGAAAAACGc                                                                      >  1:3065350/1‑101 (MQ=255)
                            gCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGAG..TCGGTCTTGGTGATCAGACAGGACTCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGTAAAGGc                                                                      >  2:4651843/1‑101 (MQ=255)
                             cACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                                                     >  1:5751107/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                    <  1:511804/101‑1 (MQ=255)
                              aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                    >  1:459387/1‑101 (MQ=255)
                               ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                  >  2:4713858/1‑101 (MQ=255)
                               ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                  >  1:1865736/1‑101 (MQ=255)
                               ccttccttCATCTTGACGAAAAAGGGGTTG..GTATCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                  >  1:5427846/1‑101 (MQ=255)
                                  tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                               >  2:5678019/1‑99 (MQ=255)
                                  tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                               >  1:23238/1‑99 (MQ=255)
                                  tccttcATCTTGACGAAAAACGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCacggc                                                                <  1:6840917/101‑5 (MQ=255)
                                     ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                            <  1:3192140/101‑6 (MQ=255)
                                     ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                            >  1:5479165/1‑96 (MQ=255)
                                     ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                            >  1:1434676/1‑96 (MQ=255)
                                      tcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                            <  1:155025/101‑1 (MQ=255)
                                      tcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                            >  1:4264721/1‑101 (MQ=255)
                                        aTCTTGACGAAAAATGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                         <  1:6928999/101‑1 (MQ=255)
                                        aTCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                         >  1:4019831/1‑101 (MQ=255)
                                         tCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                        >  1:1997928/1‑101 (MQ=255)
                                          cTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                       >  1:2411273/1‑101 (MQ=255)
                                          cTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                       <  2:6738860/101‑1 (MQ=255)
                                                     aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                             <  1:1136998/91‑1 (MQ=255)
                                                     aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                             <  2:1182343/91‑1 (MQ=255)
                                                      ggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                            <  2:4008448/92‑1 (MQ=255)
                                                       gggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg                                           >  1:6053924/9‑101 (MQ=255)
                                                        ggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                         <  1:980549/94‑1 (MQ=255)
                                                         gttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                        >  1:6263395/7‑101 (MQ=255)
                                                         gttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                        <  1:5898081/95‑1 (MQ=255)
                                                          ttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                       >  1:61377/6‑100 (MQ=255)
                                                          ttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                       >  2:4459459/6‑100 (MQ=255)
                                                           tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                      >  2:1875737/5‑99 (MQ=255)
                                                           tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                      >  2:5128034/5‑99 (MQ=255)
                                                            ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGGAGAGGCCCCCAACAGGGCCAG.tttgc                                     >  2:3263516/4‑98 (MQ=255)
                                                            ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                     >  2:6226432/4‑98 (MQ=255)
                                                            ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                     >  1:6430006/4‑98 (MQ=255)
                                                             gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                    >  1:3127068/3‑97 (MQ=255)
                                                             gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                    >  2:6173987/3‑97 (MQ=255)
                                                             gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                    <  2:6903137/99‑5 (MQ=255)
                                                              tg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                   >  2:2357910/2‑96 (MQ=255)
                                                              tg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGAGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                   >  2:3433756/2‑96 (MQ=255)
                                                               g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                   >  2:586059/1‑101 (MQ=255)
                                                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                  >  1:903370/1‑101 (MQ=255)
                                                                      tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                              <  1:4495302/91‑1 (MQ=255)
                                                                      tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                              <  1:781896/91‑1 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                             >  1:249548/10‑101 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                             <  2:1985521/92‑1 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                             <  2:1357127/92‑1 (MQ=255)
                                                                       cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                             >  2:1099224/10‑101 (MQ=255)
                                                                        ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                            >  2:1790202/9‑101 (MQ=255)
                                                                        ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                            <  2:5291157/93‑1 (MQ=255)
                                                                          ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                          >  1:1333646/7‑101 (MQ=255)
                                                                            tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                        >  1:1175988/5‑101 (MQ=255)
                                                                               tcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                     >  1:6704821/2‑101 (MQ=255)
                                                                                cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                    >  2:5005000/1‑101 (MQ=255)
                                                                                 agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                   <  1:4936091/101‑1 (MQ=255)
                                                                                 agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                   >  2:6083800/1‑101 (MQ=255)
                                                                                 agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                   <  2:1434626/101‑1 (MQ=255)
                                                                                  gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                  <  2:2411179/101‑1 (MQ=255)
                                                                                     agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc               <  2:3951730/101‑1 (MQ=255)
                                                                                      ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg              >  2:925732/1‑101 (MQ=255)
                                                                                        ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt            <  1:1582301/101‑1 (MQ=255)
                                                                                        caatacatccGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt            >  2:5894119/10‑101 (MQ=255)
                                                                                         cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt           <  1:4358457/101‑1 (MQ=255)
                                                                                         cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt           <  2:2136897/101‑1 (MQ=255)
                                                                                          ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc          <  1:206918/101‑1 (MQ=255)
                                                                                          ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc          <  2:639662/101‑1 (MQ=255)
                                                                                           cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa         <  2:3493950/101‑1 (MQ=255)
                                                                                             gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt       <  1:2358742/101‑1 (MQ=255)
                                                                                             gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt       <  2:3803854/101‑1 (MQ=255)
                                                                                             gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt       >  2:2165795/1‑101 (MQ=255)
                                                                                              tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg      <  2:5436323/101‑1 (MQ=255)
                                                                                               ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt     >  2:3913505/1‑101 (MQ=255)
                                                                                                tCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc    <  2:4125282/101‑1 (MQ=255)
                                                                                                 cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg   <  2:4780238/101‑1 (MQ=255)
                                                                                                       |                                                                                               
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGG  >  NC_014034/2177218‑2177405

Base quality scores:  ATCG  < 3 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG