Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 1077.2 288coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (156/131):  ref base (0/0):  total (157/131)

Only 100 of 320 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCT  >  NC_014034/2177248‑2177433
                                                                                                        |                                                                                             
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                >  1:459387/1‑101 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTG..GTATCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                                              >  1:5427846/1‑101 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                                             <  2:539091/101‑1 (MQ=255)
     ccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                                                          <  1:5762143/101‑4 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                         <  2:3049586/101‑5 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                        >  1:1434676/1‑96 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                        >  2:3360248/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                       >  2:6437668/1‑101 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                                                   <  1:2372150/101‑1 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                                                   >  2:1299/1‑101 (MQ=255)
                       aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                                                         <  2:1865664/91‑1 (MQ=255)
                          ggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                                                     <  1:1767574/94‑1 (MQ=255)
                           gttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAgct                                                                     >  1:916842/7‑99 (MQ=255)
                           gttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                                                    >  1:6263395/7‑101 (MQ=255)
                            ttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                                                   >  2:4459459/6‑100 (MQ=255)
                            ttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                                                   >  2:4675264/6‑100 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                  <  1:4297094/97‑3 (MQ=255)
                              ttggtgccggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCATCAGGGCCAG.ttt                                                                   >  1:7003396/8‑100 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                 >  2:6226432/4‑98 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                >  2:6173987/3‑97 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                >  1:3127068/3‑97 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                <  2:6903137/99‑5 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                >  2:2192588/3‑97 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                >  2:779587/3‑97 (MQ=255)
                                tg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                               >  2:2357910/2‑96 (MQ=255)
                                tg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                               >  2:4752717/2‑96 (MQ=255)
                                     cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                             >  1:6111533/1‑101 (MQ=255)
                                         cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                         >  1:249548/10‑101 (MQ=255)
                                         cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                         >  2:4265960/10‑101 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                        <  2:4951004/93‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                        <  2:5014537/93‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                        >  1:4061098/9‑101 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGCTTGCGCATCATCAc                                                        >  1:5085754/9‑101 (MQ=255)
                                          ttggtaatcagacagGCCCCGGTTTCGGCCAA.AGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                        <  2:2792634/93‑1 (MQ=255)
                                           tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                       >  1:2033523/8‑101 (MQ=255)
                                           tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                       <  1:4246466/94‑1 (MQ=255)
                                            ggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                      >  1:1333646/7‑101 (MQ=255)
                                            ggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                      >  2:1401518/7‑101 (MQ=255)
                                             gtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGt                                                     >  2:300337/6‑101 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                    >  1:4970288/5‑101 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                    <  1:2328783/97‑1 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                    >  1:1175988/5‑101 (MQ=255)
                                               gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                   >  2:2149621/4‑101 (MQ=255)
                                                atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                                                  >  2:197294/3‑101 (MQ=255)
                                                atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                                                  <  1:3560558/99‑1 (MQ=255)
                                                 tcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                                                 <  2:6697567/100‑1 (MQ=255)
                                                      cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                            <  2:6111221/101‑1 (MQ=255)
                                                      cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                            >  1:4914882/1‑101 (MQ=255)
                                                      cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                            >  1:4388805/1‑101 (MQ=255)
                                                       agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                           <  2:2527028/101‑1 (MQ=255)
                                                       agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                           <  1:5218901/101‑1 (MQ=255)
                                                       agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                           >  1:1970353/1‑101 (MQ=255)
                                                       agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                           <  2:3951730/101‑1 (MQ=255)
                                                        ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                          <  2:4187806/101‑1 (MQ=255)
                                                          ccccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                        <  1:1582301/101‑1 (MQ=255)
                                                          caatacatccGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                        >  2:5894119/10‑101 (MQ=255)
                                                            ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                      <  2:639662/101‑1 (MQ=255)
                                                            ccGGTTTCGGCC.GAAGCCCCCTCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCCACAGGGCCAGTTTGCGCATCATCACGGCCTCTCCTCCCGTTTc                                      <  1:6862521/101‑1 (MQ=255)
                                                             cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                     <  2:3493950/101‑1 (MQ=255)
                                                              ggTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                    <  2:5444782/101‑1 (MQ=255)
                                                               gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                   <  1:3208411/101‑1 (MQ=255)
                                                               gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                   <  2:3803854/101‑1 (MQ=255)
                                                                tttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                  <  2:4060922/101‑1 (MQ=255)
                                                                 ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                 >  2:3913505/1‑101 (MQ=255)
                                                                  tCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                <  2:5724390/101‑1 (MQ=255)
                                                                  tCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                <  2:2029277/101‑1 (MQ=255)
                                                                    ggCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                              >  1:6047691/1‑101 (MQ=255)
                                                                        cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                           >  2:6991808/4‑101 (MQ=255)
                                                                         gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                          >  1:6059195/3‑101 (MQ=255)
                                                                         gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                          <  1:5645362/99‑1 (MQ=255)
                                                                         gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                          >  1:1545736/3‑101 (MQ=255)
                                                                          aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccccc                         >  1:1878828/2‑100 (MQ=255)
                                                                          aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                         >  1:6724403/2‑101 (MQ=255)
                                                                          aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                         <  1:383245/100‑1 (MQ=255)
                                                                           aGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                        <  1:2784633/101‑1 (MQ=255)
                                                                            gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                       <  1:3040087/101‑1 (MQ=255)
                                                                             ccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcat                      <  2:925184/101‑3 (MQ=255)
                                                                               gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                    <  1:1030184/101‑1 (MQ=255)
                                                                               gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                    <  1:6189340/101‑1 (MQ=255)
                                                                               gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                    <  1:99212/101‑1 (MQ=255)
                                                                               gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                    <  2:102318/101‑1 (MQ=255)
                                                                                ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                   <  1:6516427/101‑1 (MQ=255)
                                                                                ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                   >  1:6617941/1‑101 (MQ=255)
                                                                                  ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                 >  2:3189027/1‑101 (MQ=255)
                                                                                   cGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                >  2:5620560/1‑101 (MQ=255)
                                                                                    ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc               >  1:4663887/1‑101 (MQ=255)
                                                                                    ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc               <  1:5908353/101‑1 (MQ=255)
                                                                                    ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc               >  1:615046/1‑101 (MQ=255)
                                                                                    ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc               >  2:4591887/1‑101 (MQ=255)
                                                                                     gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt              >  1:853995/1‑101 (MQ=255)
                                                                                     gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt              >  1:3937465/1‑101 (MQ=255)
                                                                                     gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt              <  2:1142794/101‑1 (MQ=255)
                                                                                        aGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg           <  1:4530200/101‑1 (MQ=255)
                                                                                          cgcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc         <  1:3866630/101‑1 (MQ=255)
                                                                                             ggAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa      >  2:1629147/1‑101 (MQ=255)
                                                                                              gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg     >  1:6320524/1‑101 (MQ=255)
                                                                                              gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg     >  1:2417446/1‑101 (MQ=255)
                                                                                              gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg     <  1:2157170/101‑1 (MQ=255)
                                                                                               aaaaCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt    >  2:16993/1‑101 (MQ=255)
                                                                                                aaaCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc   <  2:5290372/101‑1 (MQ=255)
                                                                                                        |                                                                                             
ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCT  >  NC_014034/2177248‑2177433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG