Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,177 +C 100% coding (866/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1771.→C100.0% 1136.0 301coding (866/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (152/148):  ref base (0/0):  total (153/148)

Only 100 of 333 total aligned reads displayed.

CGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180079‑2180275
                                                                                                     |                                                                                                  
cGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAgg                                                                                                     >  2:1812486/1‑101 (MQ=255)
  gCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.cc                                                                                                  <  1:5213840/101‑2 (MQ=255)
   cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccg                                                                                                 <  1:199274/101‑3 (MQ=255)
     tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggt                                                                                               <  2:4861889/101‑5 (MQ=255)
     tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggt                                                                                               <  1:5911199/101‑5 (MQ=255)
     tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggg                                                                                               >  1:1008766/1‑97 (MQ=255)
     tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggg                                                                                               >  2:2465767/1‑97 (MQ=255)
     tctgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggt                                                                                               >  1:694652/3‑97 (MQ=255)
      gtgGATCGATGCGGCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggtg                                                                                              <  2:4278805/101‑6 (MQ=255)
       tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGgtgt                                                                                              >  2:7101773/1‑101 (MQ=255)
       tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGgtgt                                                                                              >  2:2950119/1‑101 (MQ=255)
       tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.ccggggg                                                                                             >  2:2258222/1‑95 (MQ=255)
           tCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcc                                                                                          <  1:2575619/101‑4 (MQ=255)
           tCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCCATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcc                                                                                          <  1:3888531/101‑4 (MQ=255)
            cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgcca                                                                                         >  2:620314/1‑97 (MQ=255)
             gATGCGACCGTGGCCGCGTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgccaa                                                                                        <  2:3251749/101‑6 (MQ=255)
             gATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTcgccaa                                                                                        >  1:1087706/1‑96 (MQ=255)
              aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                                       <  1:5381363/101‑1 (MQ=255)
              aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                                       <  2:4333257/101‑1 (MQ=255)
              aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAg                                                                                       >  1:4455501/1‑101 (MQ=255)
                 cGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCAt                                                                                    <  1:3143904/101‑1 (MQ=255)
                  gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATg                                                                                   <  2:464455/101‑1 (MQ=255)
                    ccGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGACAAGCATGGc                                                                                 <  1:6231366/101‑1 (MQ=255)
                     cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCa                                                                                <  1:4412454/101‑1 (MQ=255)
                       tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAAc                                                                              <  1:5794972/101‑1 (MQ=255)
                       tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAAc                                                                              <  2:4242769/101‑1 (MQ=255)
                       tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAAc                                                                              >  2:2917095/1‑101 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             <  2:5166022/101‑1 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             >  1:4550411/1‑101 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             <  2:209576/101‑1 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             <  2:6738282/101‑1 (MQ=255)
                            gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                         >  1:5942361/1‑101 (MQ=255)
                            gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                         >  1:1582374/1‑101 (MQ=255)
                             ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                                        <  2:282221/101‑1 (MQ=255)
                             ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                                        <  1:4060589/101‑1 (MQ=255)
                              ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctct                                                                       <  1:6046919/101‑1 (MQ=255)
                               tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                      <  2:5301881/101‑1 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    <  1:5183841/101‑1 (MQ=255)
                                   tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGcc                                                                  <  2:1009669/101‑1 (MQ=255)
                                    cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                 >  1:842670/1‑101 (MQ=255)
                                    cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                 >  2:5564614/1‑101 (MQ=255)
                                     ntNNNNAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                <  1:2427718/100‑1 (MQ=255)
                                     gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                >  1:3634352/1‑101 (MQ=255)
                                       gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                              <  1:433419/101‑1 (MQ=255)
                                          cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                           <  1:6322418/101‑1 (MQ=255)
                                           aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                          >  2:6489765/1‑100 (MQ=255)
                                           aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                          <  1:5248165/101‑2 (MQ=255)
                                           aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                          >  1:188715/1‑100 (MQ=255)
                                           aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                          <  1:4692088/101‑2 (MQ=255)
                                            aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                         >  1:2385523/1‑99 (MQ=255)
                                              cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                       >  2:4524119/1‑97 (MQ=255)
                                              cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                       >  1:1206809/1‑97 (MQ=255)
                                               ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                     >  1:7130434/1‑101 (MQ=255)
                                                cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                    >  1:50371/1‑101 (MQ=255)
                                                 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                   >  1:5775520/1‑100 (MQ=255)
                                                  aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                  >  2:4711423/1‑99 (MQ=255)
                                                   ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                 >  1:4876632/1‑98 (MQ=255)
                                                    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                >  2:2008520/1‑97 (MQ=255)
                                                     aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGCA.CCTCGCC.ATTCcg                                              >  2:1524996/1‑101 (MQ=255)
                                                        cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                           >  1:1656405/1‑101 (MQ=255)
                                                         ggCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                                          >  2:537129/1‑101 (MQ=255)
                                                               ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                    >  1:6527422/1‑101 (MQ=255)
                                                                gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                   >  2:5656942/1‑101 (MQ=255)
                                                                 ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                  <  2:3431446/101‑1 (MQ=255)
                                                                 ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                  <  2:3027148/101‑1 (MQ=255)
                                                                  cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGGaaa                                 >  1:3303375/1‑101 (MQ=255)
                                                                  cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                 <  2:3506725/101‑1 (MQ=255)
                                                                   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                >  2:5353342/1‑101 (MQ=255)
                                                                    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGTGGGctatgtgacc                               <  2:1463611/101‑10 (MQ=255)
                                                                    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGAGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGTGGGctatgtgacc                               >  1:1463671/1‑92 (MQ=255)
                                                                    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGACGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                               >  1:5013444/1‑100 (MQ=255)
                                                                     tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                              >  2:2727629/1‑99 (MQ=255)
                                                                     tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                              <  1:3466824/101‑3 (MQ=255)
                                                                      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                             >  1:59795/1‑98 (MQ=255)
                                                                       aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                            >  2:2601772/1‑97 (MQ=255)
                                                                       aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggac                            >  2:5759140/1‑97 (MQ=255)
                                                                        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                          <  1:2133959/101‑1 (MQ=255)
                                                                         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                         >  1:6695578/1‑101 (MQ=255)
                                                                         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                         >  2:4443109/1‑101 (MQ=255)
                                                                           cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                       >  2:5929878/1‑101 (MQ=255)
                                                                            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      <  2:1305509/101‑1 (MQ=255)
                                                                            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      >  1:3208160/1‑101 (MQ=255)
                                                                            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      >  1:5540870/1‑101 (MQ=255)
                                                                              cttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                    <  2:4058906/95‑1 (MQ=255)
                                                                                  gcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                >  2:2419310/3‑101 (MQ=255)
                                                                                   cgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               <  1:1172521/100‑1 (MQ=255)
                                                                                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa             >  2:3888818/7‑101 (MQ=255)
                                                                                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa             <  1:6876219/95‑1 (MQ=255)
                                                                                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa             <  2:811531/95‑1 (MQ=255)
                                                                                     ctggcgccGAACAAGGCCGGTGACGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa             >  2:6855116/7‑101 (MQ=255)
                                                                                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAAGTGCCTCACGTCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGaaa            >  1:7071775/6‑101 (MQ=255)
                                                                                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa            <  2:7130060/96‑1 (MQ=255)
                                                                                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGCCAC.CGAAAAGGCCGAGTGGGGCCTGaaa            >  1:5706828/6‑101 (MQ=255)
                                                                                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt           <  1:3062684/97‑1 (MQ=255)
                                                                                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt           >  2:3839004/5‑101 (MQ=255)
                                                                                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      <  1:1812550/101‑1 (MQ=255)
                                                                                             gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg     <  1:2727731/101‑1 (MQ=255)
                                                                                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa    <  2:6021427/101‑1 (MQ=255)
                                                                                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa    <  1:6490095/101‑1 (MQ=255)
                                                                                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt  <  1:5314079/101‑1 (MQ=255)
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CGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180079‑2180275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG