Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,975 +CC 100% intergenic (+248/‑257) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,9751.→C100.0% 18.6 17intergenic (+248/‑257)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (1/15):  ref base (0/1):  total (1/16)
*NC_0140342,185,9752.→C100.0% 18.5 17intergenic (+248/‑257)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (1/15):  ref base (0/1):  total (1/16)

AAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCA  >  NC_014034/2185875‑2186067
                                                                                                         ||                                                                                              
aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..g                                                                                                  >  2:387688/1‑101 (MQ=255)
aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..g                                                                                                  <  1:4249842/101‑1 (MQ=255)
aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..g                                                                                                  <  2:3261211/101‑1 (MQ=255)
 agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGTTGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..c                                                                                               <  1:4555164/101‑1 (MQ=37)
 agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..c                                                                                               <  1:4800874/101‑1 (MQ=255)
 agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..c                                                                                               >  1:6706418/1‑101 (MQ=255)
     gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTTACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..ccccc                                                                                           <  2:3388154/101‑3 (MQ=37)
      cGGCTTGCCCCGACGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccg                                                                                          <  2:6559502/101‑4 (MQ=25)
       ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccgg                                                                                         >  2:5097781/1‑97 (MQ=25)
       ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccgg                                                                                         >  2:1095687/1‑97 (MQ=25)
         cTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGGGGG..G..Ccc..gcggcg                                                                                     >  2:399100/1‑97 (MQ=25)
          ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcgg                                                                                      >  2:1013453/1‑94 (MQ=25)
           tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcggg                                                                                     >  2:5563753/1‑93 (MQ=21)
            tccccGACGGGCTTG.TTTTGATGGCAGAAGAAACGTCGAACCGCCCCC.TTTCACCTTGGCTCAAATAGCCCCGGCGGCCAGGGGCGGC..G..cccctggcggag                                                                                    >  2:4624593/2‑92 (MQ=255)
            gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcgggg                                                                                    >  1:190550/1‑92 (MQ=21)
             ccccGAAGGCCTTG.TTTTGATGGCCCAAGAAACGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcgggtc                                                                                   <  2:7027853/101‑11 (MQ=18)
                                           aaaCGCCGAACCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcc                                                    <  2:4176276/101‑1 (MQ=2)
                                             ccGCCGACCCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGC..C..CCCCGGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                  <  1:6478459/100‑1 (MQ=2)
                                             aCGCCGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                  <  1:6207504/101‑1 (MQ=2)
                                              cGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCa                                                 <  2:5735672/101‑1 (MQ=0)
                                               gccgaccccccccc.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg                                                <  1:4208691/91‑1 (MQ=2)
                                                ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGTTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                               <  2:2203682/101‑1 (MQ=0)
                                                ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                               <  1:1013488/101‑1 (MQ=2)
                                                     ccG.CCCCTTTTCGCCTTGGCTCAAATATCCCGGGGGTCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                          <  2:5196429/101‑1 (MQ=2)
                                                           cc.TTTCCCCTTGGCTCAAATAGCCCGGGGGCCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                    <  1:73419/101‑1 (MQ=9)
                                                           cc.TTTCCCCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                    <  2:4847608/101‑1 (MQ=9)
                                                                    cggggCTCAAATACCCGGGGGGCCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                            <  1:5755541/98‑1 (MQ=2)
                                                                         cTCAAATATCCGGGGGGCCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc                       <  2:930069/101‑1 (MQ=9)
                                                                          ccAAATACCCGGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCt                      <  1:5313585/100‑1 (MQ=12)
                                                                            aaaTACCCGGGGGGTCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGt                    <  2:3569245/101‑1 (MQ=9)
                                                                             atccgccggggggTCGGGGGGCGGACCC..CCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACCCCCCAGCGCCCCCGACCGACTTGCGACGGCCTGTc                   <  1:5413274/93‑1 (MQ=2)
                                                                             aaTATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGGTGCCTCC.GGCG.AAGCCCCAGGCGGAACAACCCACCGCCCCCGACCGCCCTGCGACgggccggc                   >  2:6222610/1‑95 (MQ=2)
                                                                                   cggggggGCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaa             <  1:5564043/99‑1 (MQ=9)
                                                                                      ggggTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg          <  1:1095722/101‑1 (MQ=18)
                                                                                      ggggCCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg          <  2:4854786/101‑1 (MQ=12)
                                                                                         gTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGccc       <  2:6535218/101‑1 (MQ=18)
                                                                                              ggggCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCca  <  1:6249211/101‑1 (MQ=18)
                                                                                                         ||                                                                                              
AAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCA  >  NC_014034/2185875‑2186067

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG