Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,280 +C 100% intergenic (‑76/‑229) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,2801.→C100.0% 1291.6 347intergenic (‑76/‑229)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (172/172):  ref base (1/2):  total (173/174)

Only 100 of 394 total aligned reads displayed.

TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCC  >  NC_014034/2249180‑2249377
                                                                                                     |                                                                                                   
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                    >  2:6167756/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                   >  2:3041738/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                   >  1:398629/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                   >  2:6055935/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                   >  2:4031212/1‑101 (MQ=255)
    ggACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc.cc                                                                                                <  2:2277249/101‑1 (MQ=255)
    ggACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAACTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc.cc                                                                                                <  1:6442183/101‑1 (MQ=255)
     gACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc.ccc                                                                                               <  1:1728911/101‑2 (MQ=255)
      aCAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc.cccg                                                                                              >  1:5049348/1‑99 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGAGCACCCGGGGCAAGAAGGGGGCTCTG.cc.cccgtcc                                                                                           >  1:1373105/1‑96 (MQ=255)
          aagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCc.cc.gtccc                                                                                          >  1:3521591/1‑101 (MQ=255)
           agaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCc.cc.gtcccg                                                                                         >  1:1890917/1‑101 (MQ=255)
               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGcc                                                                                     >  2:6271493/1‑101 (MQ=255)
               cGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGcc                                                                                     <  2:5137033/101‑1 (MQ=255)
                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCggg                                                                                  <  2:1960669/101‑1 (MQ=255)
                   ggACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGa                                                                                 >  1:1663248/1‑101 (MQ=255)
                    gACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGAc                                                                                <  2:2842587/101‑1 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACt                                                                               >  2:901445/1‑101 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTc                                                                              >  2:5047101/1‑101 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTc                                                                              >  2:1687861/1‑101 (MQ=255)
                       aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTcc                                                                             >  1:5238297/1‑101 (MQ=255)
                       aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTcc                                                                             >  2:1228386/1‑101 (MQ=255)
                       aTGGTACTCGCTTTCGTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCGCC.GTCCCGTGCCGGGACTcc                                                                             >  1:5627904/1‑101 (MQ=255)
                        tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTccc                                                                            >  2:1855683/1‑101 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTcccc                                                                           <  1:3279762/101‑1 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTcccc                                                                           >  2:308221/1‑101 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTccccc                                                                          <  2:3765262/101‑1 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTccccc                                                                          <  2:4239874/101‑1 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCgg                                                                        <  1:4745751/101‑1 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCgg                                                                        <  1:2119735/101‑1 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCgg                                                                        >  1:552079/1‑101 (MQ=255)
                               cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGat                                                                     <  1:6018402/101‑1 (MQ=255)
                                 cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGatat                                                                   >  1:767480/1‑101 (MQ=255)
                                 cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGatat                                                                   <  2:5847845/101‑1 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  >  1:57521/1‑101 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  <  2:6465825/101‑1 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  >  2:6347239/1‑101 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  >  1:5332359/1‑101 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCCCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATAtt                                                                  <  1:3405019/101‑1 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                <  2:1585695/101‑1 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                >  1:6353619/1‑101 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                                <  1:6722059/101‑1 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                               >  1:1092226/1‑101 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             >  2:6916589/1‑101 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                             >  1:1003442/1‑101 (MQ=255)
                                        tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                            >  2:5465816/1‑101 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                           <  1:6056247/101‑1 (MQ=255)
                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                         <  1:4038052/101‑1 (MQ=255)
                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGa                                                        >  1:1537141/1‑101 (MQ=255)
                                             ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                       <  2:2553641/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                     <  1:663938/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                     >  2:464063/1‑101 (MQ=255)
                                                ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                    >  2:4434276/1‑101 (MQ=255)
                                                 tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                   <  2:767080/101‑1 (MQ=255)
                                                 tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                   >  1:6910931/1‑101 (MQ=255)
                                                 tGAGATGCACAGATACCGCAGCCTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                   <  2:2056521/101‑1 (MQ=255)
                                                  gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                  <  2:1186085/101‑1 (MQ=255)
                                                  gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                  <  2:4117691/101‑1 (MQ=255)
                                                      tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggggg                                              <  2:1299321/101‑1 (MQ=255)
                                                      tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTATGCCC.CC.GTCCCGTGCCGGGACTCCCTCGGGATATTTGAGGCAAGATGAAgggggg                                              <  2:4561484/101‑1 (MQ=39)
                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                             <  2:4842043/101‑1 (MQ=255)
                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGc                                             >  1:4547336/1‑101 (MQ=255)
                                                       gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTGGAGGCAAGATGAAGGGGGGc                                             >  1:4390792/1‑101 (MQ=255)
                                                        cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                            <  1:2723189/101‑1 (MQ=255)
                                                         acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCtt                                           >  2:3350441/1‑101 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                          <  2:552959/101‑1 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                          >  2:5597774/1‑101 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                         <  1:38456/101‑1 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                         <  1:4127248/101‑1 (MQ=255)
                                                             agcccGCAGCTTGGGCCCCAGGCGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                       <  2:1162897/98‑1 (MQ=37)
                                                             aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                       >  2:1482032/1‑101 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAGGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                     >  2:3406726/1‑101 (MQ=255)
                                                                ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                    <  2:6565228/101‑1 (MQ=255)
                                                                ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                    <  1:2198946/101‑1 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGGCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                <  2:5742870/101‑1 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                <  1:378241/101‑1 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                                >  1:2310053/1‑101 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                              >  1:4227783/1‑101 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                              >  2:2951317/1‑101 (MQ=255)
                                                                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaaa                            >  2:3886469/1‑101 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                           <  1:926334/101‑1 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                           >  2:5280081/1‑101 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                           <  2:2423721/101‑1 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                           >  1:4807769/1‑101 (MQ=255)
                                                                          ggCACCCGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGCAAata                          <  2:3521455/101‑1 (MQ=255)
                                                                              cccGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                      <  1:5925036/101‑1 (MQ=255)
                                                                               ccGGGGCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                     >  1:300406/1‑101 (MQ=255)
                                                                                 ggggCAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggg                   >  2:2198174/1‑101 (MQ=255)
                                                                                     cAAGAAGGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg               >  1:5161729/1‑100 (MQ=255)
                                                                                      aaggagGGGGCCCTG.CC.CCAATCCCTTGCCCGGACTCCCCCTGTATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTTCCGAAAATATCCCGGGGGGTg              <  2:677531/101‑1 (MQ=25)
                                                                                      aagaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg              >  2:2169379/1‑101 (MQ=255)
                                                                                       agaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa             <  1:1277483/101‑1 (MQ=255)
                                                                                       agaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa             >  2:2681487/1‑101 (MQ=255)
                                                                                        gaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa            >  1:547608/1‑101 (MQ=255)
                                                                                        gaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa            <  1:3082853/101‑1 (MQ=255)
                                                                                        gaagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa            <  2:7035583/101‑1 (MQ=255)
                                                                                         aagGGGGCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt           <  1:6978797/101‑1 (MQ=255)
                                                                                           gggggCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg         <  1:1188798/101‑1 (MQ=255)
                                                                                            ggggCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc        >  1:6242450/1‑101 (MQ=255)
                                                                                             gggCTCTGCCC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg       >  1:2913370/1‑101 (MQ=255)
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TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CC.CC.GTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCC  >  NC_014034/2249180‑2249377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG