BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,300	+C	1.6%	intergenic (‑96/‑209)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,300	1	.→C	1.6%	9.4 0.0 ‑0.1	376	intergenic (‑96/‑209)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (3/3): ref base (181/189): total (184/192)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.33e-01

Only 100 of 427 total aligned reads displayed.


ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTC  >  NC_014034/2249201‑2249399


 cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.c                                                                                                       >  2:5213635/1‑101 (MQ=255)


  aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.cc                                                                                                      >  2:1228386/1‑101 (MQ=255)


   tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGAGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.ccc                                                                                                     <  2:3391020/101‑1 (MQ=255)


    ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.cccc                                                                                                    <  1:1317068/101‑1 (MQ=255)


    ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.cccc                                                                                                    <  2:3882764/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.ccccc                                                                                                   <  1:5914650/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.ccccc                                                                                                   <  2:2036166/101‑1 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.ccccc                                                                                                   >  2:2092041/1‑101 (MQ=255)


     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.ccccc                                                                                                   <  2:4239874/101‑1 (MQ=255)


           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGata                                                                                             <  2:6782563/101‑1 (MQ=255)


            cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGTCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                            >  1:1547575/1‑101 (MQ=255)


             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                           >  1:57521/1‑101 (MQ=255)


              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGACAttt                                                                                          >  2:294350/1‑101 (MQ=255)


               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                         <  2:1135508/101‑1 (MQ=255)


               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                         <  1:2184343/101‑1 (MQ=255)


                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGTGACT.CCCCCGGGATATTTgg                                                                                        >  1:6948597/1‑100 (MQ=255)


                  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                      >  1:1003442/1‑101 (MQ=255)


                  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                      >  2:551654/1‑101 (MQ=255)


                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                     >  2:6118195/1‑101 (MQ=255)


                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGATT.CCCCCGGGATATTTGAGGCa                                                                                    <  1:4793157/101‑1 (MQ=255)


                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                    <  1:3017936/101‑1 (MQ=255)


                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                  <  1:4038052/101‑1 (MQ=255)


                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                 >  2:366742/1‑101 (MQ=255)


                        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                <  2:2553641/101‑1 (MQ=255)


                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAggagatcgga                                                                              >  1:5219900/1‑93 (MQ=38)


                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                              >  2:464063/1‑101 (MQ=255)


                           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                             <  1:5492042/101‑1 (MQ=255)


                               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                      >  1:5995169/1‑101 (MQ=255)


                                gtGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                     <  2:5575516/100‑1 (MQ=255)


                                aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                     >  2:1249340/1‑101 (MQ=255)


                                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGGGGc                                                                   >  1:4390792/1‑101 (MQ=255)


                                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                  <  2:6931060/101‑1 (MQ=255)


                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                <  1:672067/101‑1 (MQ=255)


                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                               >  2:1715009/1‑101 (MQ=255)


                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                               <  1:4127248/101‑1 (MQ=255)


                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                               <  2:4898866/101‑1 (MQ=255)


                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                             <  1:6306779/101‑1 (MQ=255)


                                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                            <  1:3911275/101‑1 (MQ=255)


                                           ccGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                          <  2:3509808/101‑1 (MQ=255)


                                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                          <  2:6565228/101‑1 (MQ=255)


                                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                         <  1:4523314/101‑1 (MQ=255)


                                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                       <  2:5036811/101‑1 (MQ=255)


                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                      >  2:1906171/1‑101 (MQ=255)


                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                      <  2:456697/101‑1 (MQ=255)


                                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                     >  1:6156798/1‑101 (MQ=255)


                                                 cgtGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                    <  2:735009/99‑1 (MQ=255)


                                                 cTTGGTCACCCGGGGAAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                    >  1:6863780/1‑101 (MQ=38)


                                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                    >  1:4227783/1‑101 (MQ=255)


                                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                  >  2:1649286/1‑101 (MQ=255)


                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                 <  2:2423721/101‑1 (MQ=255)


                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                 >  2:5280081/1‑101 (MQ=255)


                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                 <  1:926334/101‑1 (MQ=255)


                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                 <  1:1715073/101‑1 (MQ=255)


                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                 >  1:4654927/1‑101 (MQ=255)


                                                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                               <  2:2250676/101‑1 (MQ=255)


                                                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                              <  1:2230758/101‑1 (MQ=255)


                                                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                              <  1:5137004/101‑1 (MQ=255)


                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                            >  2:859900/1‑101 (MQ=255)


                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                            <  2:2672973/101‑1 (MQ=255)


                                                               gCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                      <  2:1056906/101‑1 (MQ=255)


                                                               gCAAGAAGGGGGCTCTG.CCCCTGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                      <  2:1181186/101‑1 (MQ=255)


                                                                cAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                     >  2:641707/1‑100 (MQ=255)


                                                                 aagaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                    >  1:5442099/1‑101 (MQ=255)


                                                                   gaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                  <  1:3794454/101‑1 (MQ=255)


                                                                    gagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                 <  1:641727/100‑1 (MQ=255)


                                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                 >  2:4112686/1‑101 (MQ=255)


                                                                    aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                 <  2:1537081/101‑1 (MQ=255)


                                                                     agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                >  2:5702431/1‑101 (MQ=255)


                                                                          gCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                           >  1:6641539/1‑101 (MQ=255)


                                                                           ctctGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                          >  2:440226/1‑101 (MQ=255)


                                                                           ctctGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                          <  1:3419422/101‑1 (MQ=255)


                                                                            tctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                         >  1:1436022/6‑101 (MQ=255)


                                                                            ctgg.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                        >  1:5598807/4‑101 (MQ=255)


                                                                             ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                        <  2:5809293/97‑1 (MQ=255)


                                                                               gccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                      >  1:1891169/3‑101 (MQ=255)


                                                                               gacccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                      >  2:2783494/3‑101 (MQ=255)


                                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                <  1:3627731/101‑1 (MQ=255)


                                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                >  2:3222722/1‑101 (MQ=255)


                                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggggg                >  1:1163344/1‑101 (MQ=255)


                                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc              <  1:3350564/101‑1 (MQ=255)


                                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc              >  1:2832057/1‑101 (MQ=255)


                                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg            >  1:603282/1‑101 (MQ=255)


                                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg            >  2:1339030/1‑101 (MQ=255)


                                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg            <  2:3073668/101‑1 (MQ=255)


                                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAAGGCGCAGCCAGAGGGGGGCAg            >  1:497490/1‑101 (MQ=255)


                                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc           >  1:2098610/1‑101 (MQ=255)


                                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc           >  2:6251473/1‑101 (MQ=255)


                                                                                            tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg          <  2:684197/101‑1 (MQ=255)


                                                                                            caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc              >  1:1071622/6‑101 (MQ=18)


                                                                                               gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAgc           <  1:3722323/99‑1 (MQ=18)


                                                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc       >  1:316258/1‑101 (MQ=255)


                                                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc       >  1:2353522/1‑101 (MQ=255)


                                                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccc     >  1:3079255/1‑101 (MQ=255)


                                                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccc     <  1:1228434/101‑1 (MQ=255)


                                                                                                  gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccc    <  2:5441827/101‑2 (MQ=255)


                                                                                                  gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccc    <  1:294354/101‑2 (MQ=255)


                                                                                                   aCT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct   <  2:619111/101‑3 (MQ=255)


                                                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct   <  1:5280347/101‑3 (MQ=255)


                                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt  <  2:603262/101‑4 (MQ=255)


                                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt  <  1:741631/101‑4 (MQ=255)


ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTC  >  NC_014034/2249201‑2249399

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG