Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,331 +A 1.9% intergenic (‑127/‑178) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3312.→A 1.9% 18.0 0.0 ‑0.7362intergenic (‑127/‑178)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (181/174):  total (185/177)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.88e-01

Only 100 of 392 total aligned reads displayed.

GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430
                                                                                                         |                                                                                                           
gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                                                              >  1:5995169/1‑101 (MQ=255)
   gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGG..G..Gc                                                                                                       >  1:4390792/1‑101 (MQ=255)
    cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCt                                                                                                      <  1:2723189/101‑1 (MQ=255)
     acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCtt                                                                                                     <  2:3055129/101‑1 (MQ=255)
     acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCtt                                                                                                     >  1:289927/1‑101 (MQ=255)
      caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCttt                                                                                                    <  1:5867733/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTc                                                                                                   <  1:4127248/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTc                                                                                                   >  2:6791554/1‑101 (MQ=255)
           aCCGCAGCTTGGGCACCCGGGGCAGGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCt                                                                                               >  2:3406726/1‑101 (MQ=255)
             cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTg                                                                                             <  1:4523314/101‑1 (MQ=255)
                  cTTGGTCACCCGGGGAAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                        >  1:6863780/1‑101 (MQ=38)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                        >  1:4227783/1‑101 (MQ=255)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaa                                                                                        >  2:2951317/1‑101 (MQ=255)
                   ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaaa                                                                                       >  2:355352/1‑101 (MQ=255)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGaaaa                                                                                      >  2:1649286/1‑101 (MQ=255)
                     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAat                                                                                     >  2:5280081/1‑101 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATc                                                                                  >  1:382347/1‑101 (MQ=255)
                          cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATccc                                                                                >  2:859900/1‑101 (MQ=255)
                           ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                                               <  2:4546550/101‑1 (MQ=255)
                           ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCg                                                                               <  2:1173185/101‑1 (MQ=255)
                            cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgg                                                                              <  2:461277/101‑1 (MQ=255)
                            cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgg                                                                              <  2:561713/101‑1 (MQ=255)
                             ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                             >  2:2198174/1‑101 (MQ=255)
                             ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                             >  2:4336443/1‑101 (MQ=255)
                               ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggggg                                                                           >  2:6919529/1‑101 (MQ=255)
                                   agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                       >  2:2681487/1‑101 (MQ=255)
                                   agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                       <  1:366746/101‑1 (MQ=255)
                                     aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    <  2:1537081/101‑1 (MQ=255)
                                     aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                    >  2:4112686/1‑101 (MQ=255)
                                      agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.Tg                                                                   >  2:6160690/1‑101 (MQ=38)
                                      agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                   <  2:2666785/101‑1 (MQ=255)
                                       gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                  >  2:5819885/1‑101 (MQ=255)
                                       gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                  <  1:1188798/101‑1 (MQ=255)
                                        agggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                 <  1:5466088/100‑1 (MQ=255)
                                         gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                <  1:1009599/101‑1 (MQ=255)
                                          ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGTGGTGAA.TGgcgc                                                               <  1:5131447/101‑1 (MQ=255)
                                            ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                           <  1:3419422/101‑1 (MQ=255)
                                             tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                          >  1:1436022/6‑101 (MQ=255)
                                                gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                       >  2:5410155/3‑101 (MQ=255)
                                                gaccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                       >  2:2783494/3‑101 (MQ=255)
                                                   cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                    >  2:3838024/1‑101 (MQ=255)
                                                    ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                   <  1:1987405/101‑1 (MQ=255)
                                                     cctcccggGCCGGGGCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                  <  2:6499042/99‑1 (MQ=255)
                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                 >  2:3222722/1‑101 (MQ=255)
                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                <  2:1059368/101‑1 (MQ=255)
                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                               <  1:3350564/101‑1 (MQ=255)
                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                               <  1:3041851/101‑1 (MQ=255)
                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                              >  1:1727764/1‑101 (MQ=255)
                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                             >  1:603282/1‑101 (MQ=255)
                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                            >  2:1023740/1‑101 (MQ=255)
                                                            tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                           <  2:684197/101‑1 (MQ=255)
                                                            caagcggACTCCCCCCGGGATATTTGGAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggga                                               >  1:4752660/6‑100 (MQ=18)
                                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                                         >  2:4909331/1‑101 (MQ=255)
                                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGAGAA..GCCAGAGGGGGGCAgcg.c                                         >  1:5228563/1‑101 (MQ=255)
                                                              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                                        <  2:1744695/101‑1 (MQ=255)
                                                               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                       >  1:2353522/1‑101 (MQ=255)
                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                      <  2:5556588/101‑1 (MQ=255)
                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                      <  1:6323577/101‑1 (MQ=255)
                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                     <  1:2951426/101‑1 (MQ=255)
                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                     >  2:6246519/1‑101 (MQ=255)
                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                   <  1:6672530/101‑3 (MQ=255)
                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                   >  1:3128543/1‑99 (MQ=255)
                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TTGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                  <  2:1314294/101‑4 (MQ=255)
                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                  <  1:741631/101‑4 (MQ=255)
                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                  <  2:603262/101‑4 (MQ=255)
                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                  <  2:1236503/101‑4 (MQ=255)
                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                 <  1:6739474/101‑5 (MQ=255)
                                                                        ccccGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tg                                  >  1:6246344/1‑100 (MQ=0)
                                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTcgc                               <  2:3106532/101‑1 (MQ=255)
                                                                          ccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgg                             <  1:208961/101‑1 (MQ=255)
                                                                           cGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgccc                               >  1:1102536/1‑97 (MQ=0)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                           <  1:2171587/101‑1 (MQ=255)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                           >  2:1144534/1‑101 (MQ=255)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                           <  1:355356/101‑1 (MQ=255)
                                                                            gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TAGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggc                           >  2:2394347/1‑101 (MQ=255)
                                                                             ggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcg                          >  2:191590/1‑101 (MQ=255)
                                                                              gATATTTGAGCCAAGATGAA...GGGG..G..GCTTTCAGCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcgg                         <  2:717367/101‑1 (MQ=25)
                                                                                 atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcg                      >  1:481809/1‑101 (MQ=255)
                                                                                 atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcg                      >  1:533331/1‑101 (MQ=255)
                                                                                  tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                     <  2:4182580/101‑1 (MQ=255)
                                                                                  tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                     <  2:3958137/101‑1 (MQ=255)
                                                                                   ttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg                    <  2:6668292/101‑1 (MQ=255)
                                                                                      aGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcg                 <  2:547596/101‑1 (MQ=255)
                                                                                      aGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcg                 >  2:5770225/1‑101 (MQ=255)
                                                                                           aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACgcgcgc            >  1:6603896/1‑101 (MQ=255)
                                                                                            gATGGA...GGGG..G..GCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc           <  1:1593521/101‑1 (MQ=37)
                                                                                            gATGAA...GGGG..G..TCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc           <  2:4390570/101‑1 (MQ=255)
                                                                                            gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc           >  1:1339850/1‑101 (MQ=255)
                                                                                             aTGAA...GGGG..T..GCGTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg          <  2:1376315/101‑1 (MQ=25)
                                                                                             aTGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg          >  2:776742/1‑101 (MQ=255)
                                                                                             aTGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg          >  1:7052704/1‑101 (MQ=255)
                                                                                              tGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccgc         <  1:2206746/101‑1 (MQ=255)
                                                                                               gAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa        <  1:6134862/101‑1 (MQ=255)
                                                                                                 a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAAc      >  2:2892132/1‑101 (MQ=255)
                                                                                                     gggg..g..gCTTTCATCTTGCCGCAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCCACa     <  1:5598064/101‑1 (MQ=25)
                                                                                                     gggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa     >  1:4009044/1‑101 (MQ=255)
                                                                                                     gggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa     <  1:3782101/101‑1 (MQ=255)
                                                                                                       gg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCATTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg   <  1:5007893/101‑1 (MQ=255)
                                                                                                        g..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt  <  2:5049094/101‑1 (MQ=255)
                                                                                                        g..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGCGCGGCCGCGACGCGCGCCGCAACAGGt  <  2:2062465/101‑1 (MQ=255)
                                                                                                         |                                                                                                           
GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG