Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,374 +G 1.7% intergenic (‑170/‑135) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3742.→G 1.7% 10.4 ‑1.5 ‑0.2385intergenic (‑170/‑135)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (198/179):  total (203/182)
Fisher's exact test strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.81e-01

Only 100 of 412 total aligned reads displayed.

GCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGC  >  NC_014034/2249275‑2249473
                                                                                                            |                                                                                                      
 ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                                                      >  1:4492496/1‑101 (MQ=255)
  ctgg.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                    >  1:5598807/4‑101 (MQ=255)
   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                    >  1:3296881/5‑101 (MQ=255)
   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                    >  1:1474279/5‑101 (MQ=255)
   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                                    >  1:4146978/5‑101 (MQ=255)
    tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                                   <  2:1047666/98‑1 (MQ=255)
    tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                                   <  1:5088714/98‑1 (MQ=255)
     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                                  >  2:2925381/3‑101 (MQ=255)
     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                                  >  1:1891169/3‑101 (MQ=255)
     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                                  <  2:1112281/99‑1 (MQ=255)
      cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                                 <  1:5260297/100‑1 (MQ=255)
       ccccCTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                                <  2:2304837/101‑1 (MQ=255)
        cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                                               >  1:5460727/1‑101 (MQ=255)
         ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                              <  1:1987405/101‑1 (MQ=255)
            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                           <  2:4114806/101‑1 (MQ=255)
            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                           >  1:6248853/1‑101 (MQ=255)
             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                          >  2:5818469/1‑101 (MQ=255)
             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                          <  1:2169459/101‑1 (MQ=255)
              ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                                         >  2:5024477/1‑101 (MQ=255)
               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                        <  2:6706884/101‑1 (MQ=255)
               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                        <  2:3073668/101‑1 (MQ=255)
               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                        <  1:1649347/101‑1 (MQ=255)
               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                        >  2:1339030/1‑101 (MQ=255)
               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAg                                                                                        >  1:497490/1‑101 (MQ=255)
                gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                       <  1:4434498/101‑1 (MQ=255)
                gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                       >  1:2098610/1‑101 (MQ=255)
                 caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAAGATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                          >  1:3373146/6‑101 (MQ=18)
                     gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.c                                                                                >  1:5891997/1‑101 (MQ=255)
                      ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cc                                                                               <  2:5238031/101‑1 (MQ=255)
                      ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cc                                                                               <  2:569645/101‑1 (MQ=255)
                      ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cc                                                                               >  1:3638035/1‑101 (MQ=255)
                       gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccc                                                                              <  1:294354/101‑2 (MQ=255)
                        aCT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                                             <  2:619111/101‑3 (MQ=255)
                        aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                                             <  1:6672530/101‑3 (MQ=255)
                        aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                                             >  1:3128543/1‑99 (MQ=255)
                        aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                                             <  2:68211/101‑3 (MQ=255)
                         cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccctt                                                                            <  2:603262/101‑4 (MQ=255)
                         cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccctt                                                                            <  2:6863426/101‑4 (MQ=255)
                         cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccctt                                                                            <  2:1236503/101‑4 (MQ=255)
                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAC.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttc                                                                           >  2:4592005/1‑97 (MQ=255)
                            cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttcg                                                                          >  1:4684026/1‑96 (MQ=255)
                            cccccGGAATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAGCTGGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttcg                                                                          <  1:6161009/101‑6 (MQ=25)
                               ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCgcgg                                                                        >  2:2854614/1‑101 (MQ=255)
                                cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCC.CCtgccc                                                                          >  1:1102536/1‑97 (MQ=0)
                                cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCC.CCtgccc                                                                          >  2:7123796/1‑97 (MQ=0)
                                    atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGggcggc                                                                   >  1:6078253/1‑101 (MQ=255)
                                     tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGcc                                                                  <  2:5411508/101‑1 (MQ=255)
                                      atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCcg                                                                 <  1:2418644/101‑1 (MQ=255)
                                           aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcg                                                            <  2:547596/101‑1 (MQ=255)
                                           aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcg                                                            >  2:1909981/1‑101 (MQ=255)
                                            ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgc                                                           <  1:1378941/101‑1 (MQ=255)
                                               aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgcgcg                                                        >  2:3921484/1‑101 (MQ=255)
                                                aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACgcgcgc                                                       >  1:6603896/1‑101 (MQ=255)
                                                 gATGGA...GGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc                                                      <  1:1593521/101‑1 (MQ=37)
                                                 gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgcc                                                      >  2:2428170/1‑101 (MQ=255)
                                                  aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGagccg                                                     >  2:840316/1‑101 (MQ=37)
                                                    gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGCGGTTAGCG.CCCACCTTCGCGGAGGGTCGCGACGCGCGCCGCa                                                   >  2:2760385/1‑101 (MQ=25)
                                                     aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCCTCGCGGGCGGCCGCGCCGCGGGCCGCaa                                                  >  2:4330687/1‑101 (MQ=25)
                                                           ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                               >  1:673427/1‑101 (MQ=255)
                                                             g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                             >  1:3438974/1‑101 (MQ=255)
                                                             g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGCGCGGCCGCGACGCGCGCCGCAACAGGt                                             <  2:2062465/101‑1 (MQ=255)
                                                                    ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                        >  1:3966207/1‑101 (MQ=255)
                                                                     tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                       <  2:5934343/101‑1 (MQ=255)
                                                                      cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                      >  2:4388175/1‑101 (MQ=255)
                                                                      cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                      <  1:1769225/101‑1 (MQ=255)
                                                                       aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                     <  1:1185207/101‑1 (MQ=255)
                                                                        tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                    >  2:650504/1‑101 (MQ=255)
                                                                         cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                   >  1:4331909/1‑101 (MQ=255)
                                                                         cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                   >  1:4846329/1‑101 (MQ=255)
                                                                          ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                  <  2:4787661/101‑1 (MQ=255)
                                                                          tggCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                  <  1:2718445/99‑1 (MQ=255)
                                                                           tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                 <  2:2533459/101‑1 (MQ=255)
                                                                             ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                               <  2:402648/101‑1 (MQ=255)
                                                                             ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                               <  1:859924/101‑1 (MQ=255)
                                                                              cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                              <  2:6273172/101‑1 (MQ=255)
                                                                              cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGCCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                              >  2:3119263/1‑101 (MQ=255)
                                                                               gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                             <  1:5751657/101‑1 (MQ=255)
                                                                               gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                             <  1:3838187/101‑1 (MQ=255)
                                                                                 aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                           >  2:3185353/1‑101 (MQ=255)
                                                                                 aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                           >  1:3764805/1‑101 (MQ=255)
                                                                                  aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                          >  1:3570184/1‑101 (MQ=255)
                                                                                     atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                       >  2:3488088/1‑101 (MQ=255)
                                                                                       cccGGGGGGTGGA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                     <  2:3713814/101‑1 (MQ=255)
                                                                                          ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                  <  2:5627614/101‑1 (MQ=255)
                                                                                            ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                >  1:735699/1‑101 (MQ=255)
                                                                                              ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg              >  2:776699/1‑101 (MQ=255)
                                                                                               gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt             <  2:2175832/101‑1 (MQ=255)
                                                                                               gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt             >  1:6776734/1‑101 (MQ=255)
                                                                                               gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt             <  1:1319463/101‑1 (MQ=255)
                                                                                                 gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa           >  1:2250550/1‑101 (MQ=255)
                                                                                                 gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa           >  1:1085864/1‑101 (MQ=255)
                                                                                                 gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa           <  1:1023775/101‑1 (MQ=255)
                                                                                                  aa.TGGCGCG..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt          <  2:1555866/101‑1 (MQ=255)
                                                                                                   a.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCCTGTCATa         >  1:2850390/1‑101 (MQ=255)
                                                                                                     tGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa        <  2:1399046/101‑1 (MQ=255)
                                                                                                     tGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa        <  1:6966959/101‑1 (MQ=255)
                                                                                                         gcA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg    >  2:3799744/1‑101 (MQ=255)
                                                                                                         gcA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg    <  1:3041068/101‑1 (MQ=255)
                                                                                                          cA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg   >  2:1731007/1‑101 (MQ=255)
                                                                                                           a..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc  >  2:1954902/1‑101 (MQ=255)
                                                                                                            |                                                                                                      
GCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGC  >  NC_014034/2249275‑2249473

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG