Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,391 +C 97.1% intergenic (‑187/‑118) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3911.→C97.1% 17.7 ‑0.7 ‑0.8305intergenic (‑187/‑118)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (159/137):  ref base (6/3):  total (165/140)
Fisher's exact test strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.51e-01

Only 100 of 376 total aligned reads displayed.

TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGT  >  NC_014034/2249291‑2249484
                                                                                                          |                                                                                              
tGCCGGGACTCCCCCGGGATATTTGAGGCAAGAGGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                                                                                 >  1:3719900/1‑101 (MQ=255)
   cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cc                                                                                            >  1:316258/1‑101 (MQ=255)
    gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccc                                                                                           <  1:6323577/101‑1 (MQ=255)
    cggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccc                                                                                           <  1:4473121/100‑1 (MQ=255)
     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                          >  1:3079255/1‑101 (MQ=255)
       aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccct                                                                                        <  1:6672530/101‑3 (MQ=255)
        cgcccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccctt                                                                                       <  2:4144243/99‑4 (MQ=255)
         tCCCCCGGGATATCTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttc                                                                                      <  2:4337424/101‑5 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTcgc                                                                                     <  2:3106532/101‑1 (MQ=255)
             ccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgg                                                                                   <  1:3788549/101‑1 (MQ=255)
              cGGGATNNGGGAGGNANNNGGANGGGN..NNNNGTCATCTTGCCGAAAAGAGCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcggg                                                                                  <  1:4935326/101‑1 (MQ=16)
              cGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCCtgccc                                                                                     >  2:7123796/1‑97 (MQ=0)
              cGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcggg                                                                                  >  2:4408840/1‑101 (MQ=255)
               gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                                                 <  1:6769494/101‑1 (MQ=255)
                ggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCCGCGCC.CCCCTTCgcgggcg                                                                                >  2:3008515/1‑101 (MQ=255)
                  atatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGGA.TGGCGCA..GCCAGAGGGGGGCAGGGCC.CCCCTTCGCCggcggc                                                                              >  1:6513119/1‑101 (MQ=38)
                   tatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                                             <  2:5411508/101‑1 (MQ=255)
                   tatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                                             >  2:5920788/1‑101 (MQ=255)
                     tttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                                           <  2:3958137/101‑1 (MQ=255)
                     tttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                                           >  2:375649/1‑101 (MQ=255)
                        gAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAc                                                                        <  1:5047355/101‑1 (MQ=255)
                              aGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACgcgcgc                                                                  <  1:4762198/101‑1 (MQ=255)
                               gATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                                 <  2:552067/101‑1 (MQ=255)
                                aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGTAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                <  2:6910577/101‑1 (MQ=255)
                                aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                                >  1:7052704/1‑101 (MQ=255)
                                aTGAAGGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGagccg                                                                >  2:840316/1‑101 (MQ=37)
                                  gAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                              >  2:6834844/1‑101 (MQ=255)
                                  gAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                              <  2:2063484/101‑1 (MQ=255)
                                   aaGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CACCCCTTCGCGGGCGGTCGCCACGGGCGCCGCaa                                                             >  2:4028710/1‑101 (MQ=25)
                                    aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                            >  2:2892132/1‑101 (MQ=255)
                                     gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                                           >  1:4009044/1‑101 (MQ=255)
                                      ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                          >  1:673427/1‑101 (MQ=255)
                                        g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                        >  1:3438974/1‑101 (MQ=255)
                                           ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                       >  2:5093846/1‑101 (MQ=255)
                                             cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                                                     <  1:293067/101‑1 (MQ=255)
                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCggg                                                    >  1:3938455/1‑99 (MQ=255)
                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                    <  1:2949020/101‑1 (MQ=255)
                                                tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                                  >  2:2562612/1‑101 (MQ=255)
                                                tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                                  >  2:2436999/1‑101 (MQ=255)
                                                 cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGCGGGGCAGCG.CACCCCTTCGCGGGCGGCCGCGGAGCGCGCCGCAACTGGTCgaccac                                                 >  2:579232/1‑96 (MQ=25)
                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                <  1:1185207/101‑1 (MQ=255)
                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                <  1:6118507/101‑1 (MQ=255)
                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                >  2:3887808/1‑101 (MQ=255)
                                                   tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                               >  2:650504/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                              >  1:2357460/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                              >  1:429545/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGATCggaagagca                                              >  1:1600311/1‑93 (MQ=25)
                                                    cTTACCGAAGTTATGCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                              <  1:2783596/101‑1 (MQ=37)
                                                     ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                             <  1:3351043/101‑1 (MQ=255)
                                                         cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                         <  2:1316993/101‑1 (MQ=255)
                                                          gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                        >  1:1861232/1‑101 (MQ=255)
                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                      >  2:3185353/1‑101 (MQ=255)
                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                      <  1:1249388/101‑1 (MQ=255)
                                                             aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                     <  1:1482092/101‑1 (MQ=255)
                                                             aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                     >  1:3570184/1‑101 (MQ=255)
                                                              atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                                    >  2:1069444/1‑101 (MQ=255)
                                                              atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                                    >  1:1361896/1‑101 (MQ=255)
                                                              atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                                    <  2:2831955/101‑1 (MQ=255)
                                                                atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                                  >  2:3488088/1‑101 (MQ=255)
                                                                  cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                <  1:6168075/101‑1 (MQ=255)
                                                                    cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                              >  1:5649607/1‑101 (MQ=255)
                                                                     ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                             >  2:1860484/1‑101 (MQ=255)
                                                                     ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                             >  1:6042181/1‑101 (MQ=255)
                                                                      gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                            <  1:1947744/101‑1 (MQ=255)
                                                                      gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                            <  1:5818771/101‑1 (MQ=255)
                                                                       ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                           <  2:2193081/101‑1 (MQ=255)
                                                                       ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                           >  1:2690359/1‑101 (MQ=255)
                                                                       ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                           >  1:735699/1‑101 (MQ=255)
                                                                        gggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                          <  2:2349527/101‑1 (MQ=255)
                                                                         ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                         >  2:776699/1‑101 (MQ=255)
                                                                           tGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGGCGCACCCATGTc                       <  1:5126367/101‑1 (MQ=255)
                                                                            gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                      >  2:6197319/1‑101 (MQ=255)
                                                                            gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                      <  1:1023775/101‑1 (MQ=255)
                                                                             aa.TGGCGCG..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                     <  2:1555866/101‑1 (MQ=255)
                                                                             aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGGCGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                     >  2:4625112/1‑101 (MQ=255)
                                                                             aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                     <  1:17518/101‑1 (MQ=255)
                                                                             aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                     >  2:1937155/1‑101 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                    >  2:6724162/1‑101 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                    >  2:479309/1‑101 (MQ=255)
                                                                                tGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                   >  2:5639592/1‑101 (MQ=255)
                                                                                 ggCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                  >  1:1090758/1‑101 (MQ=255)
                                                                                    gcA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg               >  1:4576935/1‑101 (MQ=255)
                                                                                     cA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg              >  2:1731007/1‑101 (MQ=255)
                                                                                     cA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg              >  1:4393090/1‑101 (MQ=255)
                                                                                      a..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc             <  2:1980247/101‑1 (MQ=255)
                                                                                           cAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc          >  2:5443691/1‑101 (MQ=255)
                                                                                           cAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc          <  2:6248534/101‑1 (MQ=255)
                                                                                           cAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc          >  1:224702/1‑101 (MQ=255)
                                                                                             gagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt        <  2:3616839/101‑1 (MQ=255)
                                                                                             gagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt        <  1:901469/101‑1 (MQ=255)
                                                                                             gagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt        <  1:5573573/101‑1 (MQ=255)
                                                                                             gagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt        <  1:2166484/101‑1 (MQ=255)
                                                                                             gagGGGGCCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt        <  2:5994864/101‑1 (MQ=255)
                                                                                              agGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc       <  1:2092121/101‑1 (MQ=255)
                                                                                              agGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc       <  1:6919883/101‑1 (MQ=255)
                                                                                              agGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc       >  2:1151181/1‑101 (MQ=255)
                                                                                               ggggggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCg      >  2:2717926/1‑101 (MQ=255)
                                                                                                 ggggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt    <  1:5410427/101‑1 (MQ=255)
                                                                                                  gggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATg   >  2:6117975/1‑101 (MQ=255)
                                                                                                   ggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGt  >  2:145337/1‑101 (MQ=255)
                                                                                                          |                                                                                              
TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGT  >  NC_014034/2249291‑2249484

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG