Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,308,282 +C 95.5% coding (515/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,308,2821.→C95.5% 16.3 ‑2.0 ‑1.2177coding (515/1203 nt)RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (73/96):  ref base (6/2):  total (79/98)
Fisher's exact test strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.78e-02

Only 100 of 188 total aligned reads displayed.

CCGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAAGGAATGCCGATCTTCTGCTTG.CCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAGG  >  NC_014034/2308181‑2308377
                                                                                                      |                                                                                               
ccGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTg                                                                                                  <  2:6245118/101‑1 (MQ=255)
 cGTCAGCTGCAGCCCGCGTTTCGCAAGCGCGGCCTCCAGGGCCGCAAGCCCGGCCCGGCCGCAACTGTGGTCCTGCTAAA.GAATGCCCATCTTCTGCTTga                                                                                                 >  1:1497027/1‑100 (MQ=255)
    cAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTG.cccc                                                                                             >  1:1071763/1‑100 (MQ=255)
     aGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTG.ccccg                                                                                            <  2:2091995/101‑3 (MQ=255)
      gCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTG.CCCcgg                                                                                           <  2:5305809/101‑1 (MQ=255)
        tGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTG.CCCcgggt                                                                                         >  1:729694/1‑99 (MQ=255)
        tGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTG.CCCcgggt                                                                                         >  1:2135986/1‑99 (MQ=255)
         gCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGtc                                                                                         >  1:5840760/1‑101 (MQ=255)
         gCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGtc                                                                                         <  2:5006147/101‑1 (MQ=255)
          cAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGtct                                                                                        <  2:5512486/101‑1 (MQ=255)
            gcccgttgttCGCAAGCGCCGCCTCCGGCCCCGCAAGCCAGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCg                                                                                      <  2:3309725/93‑1 (MQ=255)
            gCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCg                                                                                      <  2:2179916/101‑1 (MQ=255)
            gCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCg                                                                                      <  1:4439713/101‑1 (MQ=255)
            gCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCg                                                                                      <  2:1751436/101‑1 (MQ=255)
                gcgTTTCGCAAGCGCCGCCTCCAGCGCCGCAAACCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCa                                                                                  <  1:3534783/101‑1 (MQ=255)
                 cgTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAg                                                                                 <  2:6039654/101‑1 (MQ=255)
                 cgTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAg                                                                                 >  1:5305972/1‑101 (MQ=255)
                  gTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGa                                                                                >  1:368387/1‑101 (MQ=255)
                   tttCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGAt                                                                               <  2:1421909/101‑1 (MQ=255)
                   ttgcGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGAt                                                                               <  2:5304777/98‑1 (MQ=255)
                    ttCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATg                                                                              >  1:3418393/1‑101 (MQ=255)
                    ttCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATg                                                                              <  2:6751616/101‑1 (MQ=255)
                    ttCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATg                                                                              >  1:204419/1‑101 (MQ=255)
                      cGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGtt                                                                            >  1:7014928/1‑101 (MQ=255)
                       gCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTg                                                                           >  1:6737142/1‑101 (MQ=255)
                       gCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTg                                                                           <  2:6997980/101‑1 (MQ=255)
                       gCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTg                                                                           >  1:1378929/1‑101 (MQ=255)
                       gCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTg                                                                           >  1:1691218/1‑101 (MQ=255)
                       gCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTg                                                                           >  1:2632942/1‑101 (MQ=255)
                        cAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGc                                                                          <  1:6072435/101‑1 (MQ=255)
                          aGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCAc                                                                        >  2:325851/1‑101 (MQ=255)
                             gccgccTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCa                                                                     <  2:2112014/101‑1 (MQ=255)
                               cgccTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAgg                                                                   <  2:5114952/101‑1 (MQ=255)
                                 ccTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcc                                                                 <  2:1260637/101‑1 (MQ=255)
                                  cTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcct                                                                <  1:2899567/101‑1 (MQ=255)
                                   tCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctc                                                               <  2:4648697/101‑1 (MQ=255)
                                    ccAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcg                                                              <  2:1173419/101‑1 (MQ=255)
                                    ccAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcg                                                              >  1:2835464/1‑101 (MQ=255)
                                     cAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcgg                                                             <  1:6456048/101‑1 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         <  2:3597738/101‑1 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         <  2:2866800/101‑1 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         <  2:2411039/101‑1 (MQ=255)
                                         gatgcAATCCCGGCCTGGCCGACACTGTCGTCGTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGCCCAGATGTTGCGCCCAggcctcggcctc                                                         <  2:2885466/98‑1 (MQ=255)
                                          ccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGgcctcggcctcg                                                        <  2:381712/101‑1 (MQ=255)
                                           cgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGcctcggcctcgg                                                       <  2:1312426/101‑1 (MQ=255)
                                            gcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCctcggcctcggc                                                      >  1:501012/1‑101 (MQ=255)
                                               aGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGcc                                                   >  1:3666046/1‑101 (MQ=255)
                                                 cccggcccggCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCAt                                                 <  1:6335100/101‑1 (MQ=255)
                                                 cccggcccggCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCAt                                                 <  2:3168756/101‑1 (MQ=255)
                                                  ccggcccggcCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATa                                                <  1:6528344/101‑1 (MQ=255)
                                                  ccggcccggcCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATa                                                >  1:245705/1‑101 (MQ=255)
                                                   cggcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATag                                               >  1:122737/1‑101 (MQ=255)
                                                    ggcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagg                                              >  1:5273283/1‑101 (MQ=255)
                                                    ggcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagg                                              <  1:4770699/101‑1 (MQ=255)
                                                     gcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagga                                             <  1:1119780/101‑1 (MQ=255)
                                                         ggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAgggg                                         >  1:4544369/1‑101 (MQ=255)
                                                            cGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGgcgc                                      <  2:77847/101‑1 (MQ=255)
                                                             gAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCa                                     <  2:6364954/101‑1 (MQ=255)
                                                             gAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCa                                     >  1:5322745/1‑101 (MQ=255)
                                                              aaaCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAg                                    >  1:2885538/1‑101 (MQ=255)
                                                              aaaCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAg                                    <  2:4168555/101‑1 (MQ=255)
                                                               aaCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAgg                                   <  2:1898997/101‑1 (MQ=255)
                                                                  tGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTg                                >  1:1014398/1‑101 (MQ=255)
                                                                   gtcgtcCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGa                               >  1:5979571/1‑101 (MQ=255)
                                                                    tcgtcCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaa                              <  1:2569145/101‑1 (MQ=255)
                                                                     cgtcCTGA.ACAAGAATGCCGATCTTCGGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaaa                             <  2:5699301/101‑1 (MQ=255)
                                                                      gtcCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaaaa                            <  2:5870029/101‑1 (MQ=255)
                                                                      gtcCTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaaaa                            <  2:2529118/101‑1 (MQ=255)
                                                                         cTGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAt                         <  2:122736/101‑1 (MQ=255)
                                                                          tGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAtt                        <  2:4544129/101‑1 (MQ=255)
                                                                          tGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAtt                        >  1:5341606/1‑101 (MQ=255)
                                                                          tGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAtt                        <  2:3547439/101‑1 (MQ=255)
                                                                          tGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAtt                        <  2:1071728/101‑1 (MQ=255)
                                                                          tGATAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAtt                        <  2:5322478/101‑1 (MQ=255)
                                                                            aTAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgc                      <  2:6548769/101‑1 (MQ=255)
                                                                            aTAAA.GAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgc                      >  1:3832732/1‑101 (MQ=255)
                                                                              taaagAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgcg                     <  2:7051894/97‑1 (MQ=255)
                                                                                aagAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCa                   >  1:3979082/3‑101 (MQ=255)
                                                                                 agAATGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCaa                  >  1:6348624/2‑101 (MQ=255)
                                                                                   aaTGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAtt                <  2:3350903/101‑1 (MQ=255)
                                                                                   aaTGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAtt                >  1:5593859/1‑101 (MQ=255)
                                                                                    aTGCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAttt               <  2:2835362/101‑1 (MQ=255)
                                                                                      gCCGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCg             >  1:6155823/1‑101 (MQ=255)
                                                                                        cGATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGa           >  1:2723485/1‑101 (MQ=255)
                                                                                         gATCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGAt          <  2:6955803/101‑1 (MQ=255)
                                                                                          aTCTTCTGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATc         <  2:1378878/101‑1 (MQ=255)
                                                                                           tcttctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcg        >  1:4943579/1‑101 (MQ=255)
                                                                                           tcttctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcg        >  1:5687175/1‑101 (MQ=255)
                                                                                            cttctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgc       >  1:450252/1‑101 (MQ=255)
                                                                                            cttctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgc       >  1:2250822/1‑101 (MQ=255)
                                                                                             ttctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgcg      <  2:5840458/101‑1 (MQ=255)
                                                                                             ttctGCTTGCCCCGGGTCTCGACCAGATGTCGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgcg      <  2:4294491/101‑1 (MQ=255)
                                                                                              tctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCgcgc     <  2:3893039/101‑1 (MQ=255)
                                                                                               ctGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCa    >  2:6067325/1‑101 (MQ=255)
                                                                                                tGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAg   >  1:374764/1‑101 (MQ=255)
                                                                                                tGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAg   >  2:4385608/1‑101 (MQ=255)
                                                                                                tGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAg   <  1:1293681/101‑1 (MQ=255)
                                                                                                tGCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGAGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAg   >  1:6418520/1‑101 (MQ=255)
                                                                                                 gCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAgg  >  2:3199198/1‑101 (MQ=255)
                                                                                                 gCTTGCCCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTAGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAgg  >  2:4862305/1‑101 (MQ=255)
                                                                                                      |                                                                                               
CCGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAAGGAATGCCGATCTTCTGCTTG.CCCGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAGG  >  NC_014034/2308181‑2308377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG