Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,330,295 +A 96.8% intergenic (+270/+42) RCAP_rcc02161 → / ← mrcA hypothetical protein/penicillin‑binding protein 1A

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,330,2954.→A96.8% 2.3 ‑2.4 0.093intergenic (+270/+42)RCAP_rcc02161/mrcAhypothetical protein/penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  new base (51/39):  ref base (0/3):  total (51/42)
Fisher's exact test strand distribution p-value = 8.85e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGG..G....GCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTCGCCTTCTTCGGGATCACCCGCT  >  NC_014034/2330195‑2330395
                                                                                                       |                                                                                                        
tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..g                                                                                                           >  1:3538518/1‑101 (MQ=255)
tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..g                                                                                                           >  1:1169066/1‑101 (MQ=255)
tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccggggggccgggg..g                                                                                                           >  2:680591/1‑101 (MQ=255)
tATTTGCGACAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccggggggccgggg..g                                                                                                           >  2:2102633/1‑101 (MQ=255)
 aTTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gc                                                                                                          >  2:113886/1‑100 (MQ=255)
 aTTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gc                                                                                                          >  1:6673074/1‑100 (MQ=255)
 aTTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gc                                                                                                          <  2:4033966/101‑2 (MQ=255)
    tGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcgga                                                                                                       >  1:6898405/1‑97 (MQ=255)
    tGCGCCAACAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcgga                                                                                                       <  2:5646693/101‑5 (MQ=255)
     gcgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggag                                                                                                      >  1:1800642/1‑96 (MQ=255)
      cgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagc                                                                                                     <  2:4880701/101‑7 (MQ=255)
      cgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagc                                                                                                     <  2:2824134/101‑7 (MQ=255)
        ccAAGAAAAAGCCGGGGCGCTCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccggggggccgggg..gcgcatccc                                                                                                   <  1:6854596/101‑9 (MQ=255)
        ccAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagccc                                                                                                   >  2:1080742/1‑93 (MQ=255)
        ccAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagccc                                                                                                   >  1:3020236/1‑93 (MQ=255)
         cAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagcccc                                                                                                  <  2:545113/101‑10 (MQ=38)
         cAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagcccc                                                                                                  >  1:5214454/1‑92 (MQ=38)
          aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagccccc                                                                                                 >  1:2913119/1‑91 (MQ=38)
          aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggg..gcggagccccc                                                                                                 <  2:488404/101‑11 (MQ=38)
          aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGT.CCGGG..G....Gcggagccccc                                                                                            <  2:3081204/101‑10 (MQ=38)
                    cGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGc                                                                                       >  2:4128519/1‑101 (MQ=25)
                        gcgcgcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAg                                                                                   <  2:324748/101‑1 (MQ=25)
                          gcgcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGc                                                                                 <  2:3696255/101‑1 (MQ=25)
                           ggcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGCCGGGTCGCCCAGGCa                                                                                <  1:6869086/100‑1 (MQ=21)
                           cgcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGGGGcc                                                                                >  2:6277288/1‑100 (MQ=21)
                            gcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGGggggg                                                                               >  1:6594473/1‑98 (MQ=21)
                             cATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATc                                                                              <  1:1453312/101‑1 (MQ=25)
                              aTGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGcccgcggagcg                                                                             >  2:2238722/1‑92 (MQ=18)
                                gCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGGCGAGGCATCGcc                                                                           >  2:59478/1‑101 (MQ=21)
                                gCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCgggggcgcc                                                                           >  1:375832/1‑94 (MQ=18)
                                gCGGGCCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGcc                                                                           <  1:4128714/101‑1 (MQ=21)
                                  ggggCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.c                                                                        <  2:1864584/101‑1 (MQ=25)
                                  ggggCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.c                                                                        <  2:3142352/101‑1 (MQ=25)
                                         cGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCCCGGGACCGCCC.CCGCGaaa                                                                 >  1:6184276/1‑101 (MQ=12)
                                             ccACCGGCCTCCCTGCCGCCTTTCCCCTGGGGCCAAAGCGCCCGGGTGTCCTGGGCCGCC..GCCCCCGGCGGCTCTCCGAGGCATCGCCT.CGGCGAAATccc                                                             <  2:6955910/100‑1 (MQ=255)
                                              cACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGGGCCCGGGGAACGCCC.CGGCGGAAAcccc                                                            >  1:4428821/1‑100 (MQ=9)
                                               cccGGCGGCCGGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCtt                                                           <  2:2620781/100‑1 (MQ=21)
                                                ccGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTa                                                          <  2:403946/101‑1 (MQ=25)
                                                ccGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGGGGCCTCGCCC.CTGCgccaaccccta                                                          >  2:4081402/1‑91 (MQ=12)
                                                   gcggcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTa                                                       <  2:6361297/101‑1 (MQ=25)
                                                   gcctcccCGGCGGGTTTCCCCTGGGGCAAAAGGACCCGGGGGGCCGGGG..GGGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTa                                                       <  1:3306320/97‑1 (MQ=12)
                                                    cggcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTaa                                                      <  2:3060830/101‑1 (MQ=25)
                                                     ggcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTaaa                                                     <  2:5061430/101‑1 (MQ=25)
                                                     ggcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGTGCCGAGGCATCGCCC.CGGCGAAAACCCCTAGTaaa                                                     >  2:6779831/1‑101 (MQ=18)
                                                     ggcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGGGCCGGGGGGTCCCCC.CGGCGGAAACCCCTTGTaaa                                                     >  1:197307/1‑101 (MQ=9)
                                                      gcCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAg                                                    <  1:6986824/101‑1 (MQ=25)
                                                       cccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGGAGCGCCC.CCGCGGAAAACCCTAGTaaaaa                                                   >  1:610043/1‑99 (MQ=9)
                                                        ccGGGCGGCTTTCCCCTGGGGCCAAAGATCCGGGGGGTCGGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAc                                                  <  1:1575091/101‑1 (MQ=21)
                                                        ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAc                                                  <  1:5821915/101‑1 (MQ=25)
                                                        ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAc                                                  <  2:2524808/101‑1 (MQ=25)
                                                        ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAc                                                  <  1:1866282/101‑1 (MQ=25)
                                                        ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCACCGCCT.CGGCGAAATCCCTTAGTAAAGAc                                                  <  1:5061324/101‑1 (MQ=21)
                                                         cGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAcc                                                 <  2:4917268/101‑1 (MQ=25)
                                                         cGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGGGCCGGGGGCTCGCCC.CCGGGGAAACCCCTAGTaaaaaac                                                 >  1:6786019/1‑97 (MQ=2)
                                                         cGGGCGGCTTCCACCGGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGAcc                                                 <  1:355288/101‑1 (MQ=21)
                                                          gggCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGGATCGCCTCCGGCAAAACCCCTT.GTaaaaaacc                                                >  2:1989063/1‑96 (MQ=9)
                                                              ggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCCAGGCATCGCCC.CCGCGCAAACCCCTAGTAAAAAccccccc                                            >  2:2372298/1‑100 (MQ=9)
                                                               gcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTGGCCGCGGCATCGCCC.CCGCGGAATCCCCTCGTTAAAAcccccccc                                           >  1:6663816/1‑99 (MQ=9)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCgg                                         <  2:3194620/101‑1 (MQ=21)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGGGGCACCGCCT.CGGCGGAATCCCCTAGTAAAGAAAGCCCCCgg                                         >  2:2683063/1‑101 (MQ=12)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CCGCCAAATCCCCTAGTAAAAACCCCCCGCgg                                         >  1:2483668/1‑101 (MQ=12)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCAGCGCCC.CCGCGAAATCCCCTCGTAAAGAactcccgccg                                         >  2:3248558/1‑99 (MQ=12)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCCGGGGATCGCCC.CGGCGAAAACCCCTTGTAAAAAACGCCCCCgg                                         >  1:796967/1‑101 (MQ=2)
                                                                 tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCCCCGCGGCATCGCCT.CCGCGCAAGACCGCAGTAAAAACCGCCCGCgg                                         >  1:7070388/1‑101 (MQ=9)
                                                                  ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGGCGAGGCATCCCCT.CCGCCAAATTCCCTTCTACAGAACCCCCGCGGa                                        >  2:2243396/1‑101 (MQ=2)
                                                                  ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGGAGCGCCT.CGGCGGAAACCCCTAGTAAAAAACGCCCGCggg                                        >  1:1524455/1‑100 (MQ=9)
                                                                  ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGCATCGCCC.CCGCGCAAAACCCTAGTAAAAAccgccccccgg                                        >  2:6556563/1‑96 (MQ=9)
                                                                  ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGGCGCCGCT.CCGCGGAATCCCCTCGTTAAGAacgccccccgc                                        >  1:5990560/1‑96 (MQ=0)
                                                                  ttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGGAGCGCCC.CCGCGGAAACCCCTAGCAAAAAACCCCCCCggg                                        >  1:73225/1‑100 (MQ=0)
                                                                   tCACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGAc                                       <  1:453115/101‑1 (MQ=21)
                                                                    cACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCC.CCGCGGAAACCCCTAGTAAAAACCGCCCGCGGGCa                                      >  2:2821029/1‑101 (MQ=12)
                                                                    cACCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCGTCGCCC.CGGCGAAAACCCCTAATAAAGAACGCCCGCGGAcc                                      >  2:3780056/1‑100 (MQ=12)
                                                                     aCCTGGGGCCAAAGATCCGGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAg                                     <  1:6576892/101‑1 (MQ=21)
                                                                     aCCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGGGGCATCGCCT.CGGCGGAAACCCCTAGTAAAAAACGCCCCCGGCCAg                                     >  1:1973895/1‑101 (MQ=9)
                                                                     aCCTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCT.CCGCGGAAACCCCTAGTCAAAAacgccccccggccg                                     >  2:5913006/1‑93 (MQ=9)
                                                                      ccTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAgg                                    <  2:3472152/101‑1 (MQ=21)
                                                                      ccTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGCAGCGCCC.CCGGGGAATCCCCTAGTAAAAAACGCCCCCGGGCacg                                    >  1:5048408/1‑99 (MQ=2)
                                                                      ccTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCT.CCGCGCAAACCCTTAGTAAAGAACGCCCGCGGACAgg                                    >  2:6442890/1‑101 (MQ=18)
                                                                       cTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAggg                                   <  1:5458412/101‑1 (MQ=21)
                                                                       cTGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGGCTCGCCC.CGGCGGAATCCCTCAGTCAAGAACGCCCCCGGCCCggg                                   >  1:2291907/1‑101 (MQ=2)
                                                                       cTGGGGCCAAAGAGCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAggg                                   <  2:3845483/101‑1 (MQ=21)
                                                                        tGGGGCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCCAGGCATCCCCG.CGGCGAAAAACCCTAGTAAAGAACGCCCCCGGGCAgcgg                                  >  2:6165805/1‑98 (MQ=2)
                                                                         ggggCCAAATGTCCCGGGGGTCGGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGAt                                 <  2:244564/101‑1 (MQ=21)
                                                                         ggggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CCGCGGCAACCCCTTGAAAAAAACGCCCGCGGAGAgcggg                                 >  2:4514859/1‑97 (MQ=0)
                                                                          gggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATc                                <  2:6898704/101‑1 (MQ=21)
                                                                          gggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CGGCGGAAACCCTTAGTAAAAAACGCCCCCGGGCAGGGagc                                >  1:2171409/1‑99 (MQ=9)
                                                                          gggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CGGCGGAAACCCCTAGTAAAGAACCCCCGCCGACAGGGaac                                >  2:2566433/1‑99 (MQ=9)
                                                                           ggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATcc                               <  2:6663474/101‑1 (MQ=21)
                                                                           ggCCAAAGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CCGCGAAAACCCCTAGTAAAAACCGCCCCCgggccagggacc                               >  2:4726457/1‑91 (MQ=9)
                                                                             ccAAAGACCCCGGGGGCCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGa                             <  1:3666250/101‑1 (MQ=21)
                                                                               aaaGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGGGCCGAGGCACCGCCC.CGGCGAAAACCCTTACTAAAAACCGCCCGCCGACAGGGATCCGCAg                           >  1:647815/1‑101 (MQ=12)
                                                                               aaaGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGCGGCATCGCCC.CGGCGAAATCCCTTAGTAAAAACCCCCCCCGGGCAGGGATCCGCAg                           >  2:3890207/1‑101 (MQ=9)
                                                                               aaaGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CCGCGAAAACCCCTAGTAAAAAACGCCCGCGGACAggggaccgcag                           >  2:3614171/1‑93 (MQ=9)
                                                                                aaGATCCCGGGGGTCGGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAgg                          <  1:4412296/101‑1 (MQ=25)
                                                                                aaGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGGATCGCCC.CGGCGAAAACCCTTAGTAAAGACCGCCCCCGGGCAGGGACCCGAAgg                          >  2:5591370/1‑101 (MQ=12)
                                                                                aaGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCC.CGGCGAAATCCCTTAGTAAAAAACCCCCCCGGACAGGGAGCCGAAgg                          >  1:582409/1‑101 (MQ=12)
                                                                                aaGATCCCGGGGGGCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAgg                          <  1:2769629/101‑1 (MQ=25)
                                                                                 aGATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGGGGCCGGGGCATCGCCC.CGGCGAAAACCCTTAGTAAAGACCGCCCGCGGGCCGGGAGCCGAAggg                         >  1:2609330/1‑100 (MQ=12)
                                                                                  gATCCCGGGGGTCCGGGG..GCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTc                        <  1:535481/101‑1 (MQ=25)
                                                                                                      g....gCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTCGCCTTCTTCGGGATCACCCGCt  <  1:4370849/101‑1 (MQ=255)
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TATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGG..G....GCCCCCGGCGGGTCGCCGAGGCATCGCCT.CGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTCGCCTTCTTCGGGATCACCCGCT  >  NC_014034/2330195‑2330395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG