Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,472,573 +G 7.2% intergenic (‑158/+25) RCAP_rcc02296 ← / ← RCAP_rcc02297 GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,472,5730.→G 7.2% 41.1 ‑0.4 ‑1.4194intergenic (‑158/+25)RCAP_rcc02296/RCAP_rcc02297GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  new base (13/1):  ref base (152/28):  total (165/29)
Fisher's exact test strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.64e-02

Only 100 of 215 total aligned reads displayed.

TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670
                                                                                                                                                                                                 
tGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgc                                                                                            >  2:199532/1‑101 (MQ=255)
 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                           <  1:4688566/101‑1 (MQ=255)
 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                           <  2:5905486/101‑1 (MQ=255)
 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                           <  1:1579689/101‑1 (MQ=255)
   gggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.cc                                                                                        <  1:6046647/101‑1 (MQ=255)
     gCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCt                                                                                      >  2:4440220/1‑101 (MQ=255)
     gCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCt                                                                                      <  1:2853477/101‑1 (MQ=255)
       tctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTcc                                                                                    >  1:4736392/1‑101 (MQ=255)
        ctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTccc                                                                                   <  1:70548/101‑1 (MQ=255)
         tGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTcccc                                                                                  <  2:5533835/101‑1 (MQ=255)
         tGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTcccc                                                                                  <  1:671985/101‑1 (MQ=255)
          gCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCg                                                                                 >  2:4625229/1‑101 (MQ=255)
           cccccAACCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGc                                                                                <  2:3554578/101‑1 (MQ=255)
           cccccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGc                                                                                <  2:2471120/101‑1 (MQ=255)
            ccccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGcc                                                                               >  2:2142568/1‑101 (MQ=255)
              ccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCt                                                                             <  2:1596876/101‑1 (MQ=255)
               cAACCCCCGGGGATATTTGCAGCAAGATGAGGGCCCGTGCTTTCATCTTGCCTCAAATACCCGGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCtt                                                                            <  2:4741972/101‑1 (MQ=37)
               aaagcccccGGGATATTGGCAGAAAGATGAAGGGCAGTGCTTTCATCTTGCCTCAAATTTCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCtt                                                                            <  2:5129907/97‑1 (MQ=37)
                caccccccGGGATTTTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTg                                                                           <  2:2298131/98‑1 (MQ=37)
                aaaCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTg                                                                           >  1:3443023/1‑101 (MQ=255)
                        gggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTg                                                                   >  2:1753650/1‑101 (MQ=255)
                        gggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTg                                                                   >  2:5378146/1‑101 (MQ=255)
                         ggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTgc                                                                  >  1:6039422/1‑101 (MQ=255)
                         ggATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTgc                                                                  >  2:916915/1‑101 (MQ=255)
                          gATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTgcg                                                                 >  2:491751/1‑101 (MQ=255)
                          gATATTTGCAGCAAGATGAAGGACCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGG..GGGCAGCA.CCCTCCCCGCCCTTGTCAGCCTgcg                                                                 >  1:1966685/1‑101 (MQ=38)
                           atatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTgcgc                                                                >  2:716451/1‑101 (MQ=255)
                            tatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCa                                                               >  2:69253/1‑101 (MQ=255)
                            tatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCa                                                               >  1:2231619/1‑101 (MQ=255)
                            tatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCa                                                               >  2:1624908/1‑101 (MQ=255)
                              tttGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAgg                                                             >  2:6527872/1‑101 (MQ=255)
                              ttggcagcaAGATGAGGGGCCTTTTTTTCTTCTTCCCACAAATCCCCGGGGTGGGGT..GGGGGGGGGCCCCCC.CCCCCCCCCCCCTTGTCAGCCTGCGCAgg                                                             <  2:2433133/98‑1 (MQ=255)
                              ttggcagaaAGATGAGGGGCGGTGTTTTCTTC.TCCCCCAAA.ATCCCCGGGGGGGGGTGGGGGGGGGGCAGCC.CCCCCCCCCCCCTTGTCAGCCTGCGCAgg                                                             <  1:1007602/98‑1 (MQ=255)
                                  cagcaAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAggcggc                                                         >  2:6204490/1‑101 (MQ=255)
                                    gcaAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGt                                                       >  1:3903428/1‑101 (MQ=255)
                                     caAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTc                                                      >  1:2348132/1‑101 (MQ=255)
                                     caAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTc                                                      >  2:1907913/1‑101 (MQ=255)
                                      aaGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     >  1:1464307/1‑101 (MQ=255)
                                         aTGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAg                                                  >  2:2354923/1‑101 (MQ=255)
                                         aTGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAg                                                  >  1:4842477/1‑101 (MQ=255)
                                           gAAGGGCCGTGCTTTCATCTTGCCTCAAACATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggc                                                >  1:5323689/1‑101 (MQ=255)
                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  1:6756518/1‑101 (MQ=255)
                                              ggcccgttttttCATCTGGCCT.AAA.ATCCCGGGGGGGGGGTGGGGGGGGGCCCGCC.CCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcggc                                             <  2:6712541/92‑1 (MQ=255)
                                                 ccGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  1:2628716/1‑101 (MQ=255)
                                                 ccGTGCTTTCATATTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  1:6342038/1‑101 (MQ=255)
                                                  cGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         >  1:2908989/1‑101 (MQ=255)
                                                  cGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         >  1:3779070/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  1:2067342/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  2:3705850/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  1:6911640/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  2:3275671/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  2:2838224/1‑101 (MQ=255)
                                                     gCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtc                                      >  1:2527403/1‑101 (MQ=255)
                                                     gCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtc                                      >  1:303129/1‑101 (MQ=255)
                                                        ttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagt                                   >  1:1593374/1‑101 (MQ=255)
                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  1:6141183/1‑101 (MQ=255)
                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  2:6447618/1‑101 (MQ=255)
                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  1:169347/1‑101 (MQ=255)
                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  2:6135781/1‑101 (MQ=255)
                                                             cTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcag                              >  1:5543811/1‑101 (MQ=255)
                                                              ttGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagt                             >  1:918543/1‑101 (MQ=255)
                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  2:5658243/1‑101 (MQ=255)
                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  1:242593/1‑101 (MQ=255)
                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  2:202070/1‑101 (MQ=255)
                                                               tGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagtt                            >  1:4896904/1‑101 (MQ=255)
                                                                gCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTc                           >  1:3720483/1‑101 (MQ=255)
                                                                 ccccAAATTGCCCGGGGGGGGGTGGGGGG..GGGCCGCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTc                           <  2:5477856/98‑1 (MQ=255)
                                                                  cTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCca                         >  1:1270947/1‑101 (MQ=255)
                                                                  cTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCca                         >  1:1392747/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcaca                       >  1:477166/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcaca                       >  1:980417/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCACCGCCCTTGTCAGCCTGCGCAGGCGGCGTGAAAGGCGGCGCGGCTCAGTTTCAGGTCcaag                       >  1:3296269/1‑99 (MQ=255)
                                                                     tcaaatCCCGGGGGGGGGGTGCGGGGGGGCCCGCC.CCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcac                        <  2:3982175/97‑1 (MQ=255)
                                                                     caaaatCCCGGGGGGGGGGTGGGGGGGGGCCCGCC.CCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcac                        <  2:4593155/97‑1 (MQ=255)
                                                                     aaaTATCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACAt                      >  2:4513548/1‑101 (MQ=255)
                                                                        tcttccGGGGGGGGGGGGGGGGGGGGGCGCCC.CCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATg                     <  1:3562297/97‑1 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  1:5881418/1‑101 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  1:633761/1‑101 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  2:6008361/1‑101 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGAGTCAAGGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  2:5255841/1‑101 (MQ=255)
                                                                         atCGCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTGAGTTTCAGTTCCACATGCgg                  >  1:5620833/1‑101 (MQ=255)
                                                                         atCCCGGGTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCgg                  >  1:5762069/1‑101 (MQ=255)
                                                                           cccGGGGGGGGGGGGGGGGGGGGCCGGCC.CCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCgg                  <  1:6262612/101‑1 (MQ=255)
                                                                              gggTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAggg             >  2:3464058/1‑100 (MQ=255)
                                                                               ggTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAt            >  1:3968765/1‑101 (MQ=255)
                                                                               ggTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAt            >  2:893102/1‑101 (MQ=255)
                                                                                gTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATc           >  1:5359746/1‑101 (MQ=255)
                                                                                gTGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATc           >  2:1395093/1‑101 (MQ=255)
                                                                                 tGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCg          >  1:3827549/1‑101 (MQ=255)
                                                                                 tGGGGTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCg          >  2:847427/1‑101 (MQ=255)
                                                                                  ggggTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGc         >  2:6305295/1‑101 (MQ=255)
                                                                                   gggTTCGGGG..AGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCa        >  1:4150555/1‑101 (MQ=255)
                                                                                      ggttcgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCAt       >  1:5494337/6‑101 (MQ=255)
                                                                                       gttcgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      >  2:5759853/5‑101 (MQ=255)
                                                                                       gttcgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      >  1:4591841/5‑101 (MQ=255)
                                                                                       gtgggggGGGGGCACCC.CCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      <  2:4302658/99‑1 (MQ=255)
                                                                                        ttcgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcg     >  1:834024/4‑101 (MQ=255)
                                                                                          cgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  2:3192599/2‑101 (MQ=255)
                                                                                          cgggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  1:4644878/2‑101 (MQ=255)
                                                                                           gggGAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgggc  >  1:4016591/1‑101 (MQ=255)
                                                                                                                                                                                                 
TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCG.CCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG