Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,056 +T 4.6% intergenic (+104/‑138) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0561.→T 4.6% 40.0 0.0 ‑1.7261intergenic (+104/‑138)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (131/118):  total (137/124)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.91e-02

Only 100 of 285 total aligned reads displayed.

GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493956‑2494152
                                                                                                     |                                                                                                      
ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGAAGCAAGATGACACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGaa                                                                                                         >  2:4653910/1‑101 (MQ=17)
 gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                       <  1:1026977/101‑1 (MQ=255)
 gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                       <  1:4792651/101‑1 (MQ=255)
  ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                      <  2:530791/101‑1 (MQ=255)
  ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                      <  1:4921060/101‑1 (MQ=255)
   gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                     <  2:6960075/101‑1 (MQ=255)
   gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTCAA.Tgg                                                                                                     <  2:3512697/101‑1 (MQ=255)
    cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                    >  1:6622282/1‑101 (MQ=255)
    cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                    >  2:1581032/1‑101 (MQ=255)
    cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                    <  1:3334951/101‑1 (MQ=255)
     gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                 >  2:5881401/1‑101 (MQ=255)
      ctccccccCGTCCGCAAGCCGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                               <  1:848607/98‑1 (MQ=18)
        gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             <  1:4131707/101‑3 (MQ=255)
            cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                         <  1:2613364/97‑7 (MQ=255)
             ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                        <  2:1889109/98‑8 (MQ=255)
                      aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCag                                                                               >  1:3415288/1‑100 (MQ=18)
                       gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                              >  1:5933131/1‑99 (MQ=18)
                       gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                              >  2:5420299/1‑99 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                             >  2:3608282/1‑98 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                             >  2:4364566/1‑98 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                             >  1:381771/1‑98 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                             >  2:5033816/1‑98 (MQ=18)
                            cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cccc                                                                       <  2:252539/101‑1 (MQ=17)
                            cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc                                                                       <  2:718542/101‑1 (MQ=17)
                             tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.c                                                                     >  2:1641865/1‑101 (MQ=17)
                              ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.cc                                                                    >  1:4038/1‑100 (MQ=17)
                              ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.cc                                                                    >  1:2407036/1‑100 (MQ=17)
                               cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.cct                                                                   <  2:3550655/101‑3 (MQ=17)
                                  ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.cctgcc                                                                <  1:994628/101‑6 (MQ=17)
                                    gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCC.CTGcccc                                                               >  1:6228942/1‑101 (MQ=0)
                                    gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCC.CTGcccc                                                               <  2:6159028/101‑1 (MQ=0)
                                     ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCC.CTGCCCcg                                                              >  1:2857575/1‑101 (MQ=2)
                                     ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCcg                                                              <  2:5653150/101‑1 (MQ=17)
                                     ggATATTTGCAGCAAGATGAAACAGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc.cctgccacg                                                             >  1:3366503/1‑93 (MQ=14)
                                      gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCcgg                                                             >  2:31145/1‑101 (MQ=18)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCC.CTGCCCcgcc                                                            <  1:6014660/101‑3 (MQ=2)
                                        tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCC.CTGCCCcgccg                                                           >  1:2739692/1‑101 (MQ=2)
                                        tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCcggcg                                                           >  1:6142998/1‑101 (MQ=17)
                                        tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCcgccg                                                           >  2:3164905/1‑101 (MQ=17)
                                          tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCcggcggg                                                         >  1:3783275/1‑100 (MQ=17)
                                          tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGt                                                         <  2:6449675/101‑1 (MQ=17)
                                          tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGt                                                         >  2:5128325/1‑101 (MQ=17)
                                           ttGCAGCAAGATTAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTc                                                        <  2:2494455/101‑1 (MQ=17)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTcc                                                       <  2:2441416/101‑1 (MQ=17)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTcc                                                       >  1:3578887/1‑101 (MQ=18)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTcc                                                       <  1:707982/101‑1 (MQ=17)
                                                    gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTggcggc                                               <  1:6883905/101‑1 (MQ=17)
                                                      tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGgcgc                                             >  2:4546162/1‑101 (MQ=18)
                                                       gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGcc                                            <  2:6581054/101‑1 (MQ=17)
                                                         aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:4070976/101‑1 (MQ=17)
                                                          aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:375861/101‑1 (MQ=17)
                                                          aCCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:1348192/101‑1 (MQ=17)
                                                           ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        >  1:4464566/1‑101 (MQ=18)
                                                            cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  1:3521948/1‑101 (MQ=18)
                                                            cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGCGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:3456692/101‑1 (MQ=17)
                                                            cGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGat                                       <  1:4085202/101‑2 (MQ=17)
                                                             ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:4260213/1‑101 (MQ=17)
                                                             ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:3847130/1‑101 (MQ=17)
                                                               aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    >  2:6118784/1‑101 (MQ=18)
                                                               aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    >  1:2970561/1‑101 (MQ=18)
                                                                tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  1:1574503/101‑1 (MQ=17)
                                                                 gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:4196347/1‑101 (MQ=17)
                                                                  ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 >  1:4061165/1‑101 (MQ=18)
                                                                   cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  2:3644775/1‑101 (MQ=18)
                                                                   cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAccggcggg                                >  2:412531/1‑101 (MQ=17)
                                                                     ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGCGGGGGGC.AGCGCCCCC.CTGCTCCGGAGGTCCGTGGCTGCGCCTTGACGCGCggggg                              >  2:5769270/1‑101 (MQ=9)
                                                                     ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCTC.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              <  1:1009584/101‑1 (MQ=18)
                                                                      tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                             >  1:5355279/1‑100 (MQ=17)
                                                                      tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:1717802/101‑1 (MQ=18)
                                                                      tCATCTGGCCCGAGATGTCGCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:6428046/101‑1 (MQ=16)
                                                                       cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  2:3206132/1‑101 (MQ=18)
                                                                        aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                           <  1:4211456/101‑1 (MQ=18)
                                                                         tcgtGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:1837078/98‑1 (MQ=18)
                                                                         cttggCCCGAAATTACCCGGGGGGGGGA.AGGCG.G.AG.CCCAGGGGGGGGCAGCC.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  1:3611082/97‑1 (MQ=255)
                                                                          cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  2:2057294/1‑101 (MQ=18)
                                                                          cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         <  2:1170636/101‑1 (MQ=18)
                                                                           ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        <  1:3914345/101‑1 (MQ=18)
                                                                              cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     >  2:2824829/1‑101 (MQ=18)
                                                                               ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:6003847/101‑1 (MQ=18)
                                                                                 gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:1978730/101‑1 (MQ=18)
                                                                                  aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAAc                 >  1:522527/1‑101 (MQ=18)
                                                                                  aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  2:2995275/101‑1 (MQ=18)
                                                                                  aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  2:2673316/1‑101 (MQ=18)
                                                                                   aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:1912982/1‑101 (MQ=21)
                                                                                   aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGGGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:609426/1‑101 (MQ=18)
                                                                                    attacccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               <  2:6304074/97‑1 (MQ=21)
                                                                                    atatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               <  2:1753697/101‑1 (MQ=21)
                                                                                     tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:4738294/1‑101 (MQ=21)
                                                                                       tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:5776040/101‑1 (MQ=21)
                                                                                         ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:1322901/101‑1 (MQ=21)
                                                                                         ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:3084703/101‑1 (MQ=21)
                                                                                         ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:6147874/101‑1 (MQ=21)
                                                                                          cGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg         <  1:6586212/101‑1 (MQ=25)
                                                                                           ggggggTGAA.TGGC..GAAG.CCGGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:319282/1‑101 (MQ=25)
                                                                                            gggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCggagttccg       >  2:2270481/1‑93 (MQ=18)
                                                                                            gggggTGAA.GGGC..GAAG.CCAGAGGGGGGCAGCCC.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:2273409/101‑1 (MQ=25)
                                                                                             ggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa      >  1:6449688/1‑101 (MQ=25)
                                                                                              gggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:2053421/101‑1 (MQ=25)
                                                                                                gTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCCC.CCCC.GTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAg   <  1:1641926/101‑1 (MQ=25)
                                                                                                 tGAA.TGGC..GAAG.CCAGAGGGCGGC.AGCG.CCCCTCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  <  1:4654149/101‑1 (MQ=37)
                                                                                                     |                                                                                                      
GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493956‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG