Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,060 +A 4.9% intergenic (+108/‑134) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0602.→A 4.9% 41.1 0.0 ‑0.8247intergenic (+108/‑134)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (126/109):  total (132/115)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 265 total aligned reads displayed.

CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152
                                                                                                      |                                                                                                  
cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                     <  2:1639586/101‑1 (MQ=255)
 gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                  >  2:5881401/1‑101 (MQ=255)
  ctccccccCGTCCGCAAGCCGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                <  1:848607/98‑1 (MQ=18)
        cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  2:6425092/97‑7 (MQ=255)
         ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                         <  1:2421694/98‑8 (MQ=255)
                 aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                                                 <  1:4841224/101‑1 (MQ=18)
                   gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                               >  1:5933131/1‑99 (MQ=18)
                    cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                              >  2:4103598/1‑98 (MQ=18)
                    cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                              >  2:4364566/1‑98 (MQ=18)
                    cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                              >  2:5033816/1‑98 (MQ=18)
                     ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAACATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                             >  1:6144098/1‑97 (MQ=18)
                      gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                          >  1:2701939/1‑101 (MQ=18)
                      gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                          >  2:30725/1‑101 (MQ=18)
                       aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                        >  2:6585192/1‑101 (MQ=18)
                       aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                        >  2:2850360/1‑101 (MQ=18)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCaccgcccc                                                                          >  2:5968552/1‑94 (MQ=18)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc                                                                       >  1:7090691/1‑101 (MQ=18)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc                                                                       <  2:718542/101‑1 (MQ=17)
                         tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.cc.cc.c                                                                     <  2:2668826/101‑1 (MQ=17)
                          ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cc                                                                    <  2:120243/101‑2 (MQ=17)
                           cccccGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cct                                                                   <  1:3292237/101‑3 (MQ=17)
                           cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cc.cct                                                                   <  2:3212537/101‑3 (MQ=17)
                               cGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.CC.CC.CTGccc                                                                >  2:3513234/1‑101 (MQ=18)
                                gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGcccc                                                               <  2:6159028/101‑1 (MQ=0)
                                gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGcccc                                                               <  2:1733916/101‑1 (MQ=17)
                                gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGcccc                                                               >  2:1766453/1‑101 (MQ=17)
                                 ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcg                                                              >  1:5969404/1‑101 (MQ=18)
                                 ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcg                                                              >  2:3475203/1‑101 (MQ=18)
                                 ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcg                                                              <  2:5653150/101‑1 (MQ=17)
                                  gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGCCCcgc                                                             >  1:6081322/1‑100 (MQ=2)
                                   atatTTGCAGCAAGATGAACCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggc                                                            <  1:6198708/101‑1 (MQ=17)
                                    tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggcg                                                           >  2:2512571/1‑101 (MQ=17)
                                      tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCcggcggg                                                         >  1:3783275/1‑100 (MQ=17)
                                       ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CC.CTGCCCcgccggat                                                        <  2:5078699/101‑3 (MQ=2)
                                       ttGCAGCAAGATTAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTc                                                        <  2:2494455/101‑1 (MQ=17)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       <  2:2441416/101‑1 (MQ=17)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       >  2:6090311/1‑101 (MQ=18)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTcc                                                       <  1:6766555/101‑1 (MQ=17)
                                            gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTgg                                                   >  1:4493287/1‑101 (MQ=18)
                                              aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGCCCGTggcg                                                 >  1:3383889/1‑101 (MQ=17)
                                               aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTggcgg                                                >  1:3811002/1‑101 (MQ=17)
                                                tggaaaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTggcggc                                               >  2:1944999/5‑101 (MQ=17)
                                                gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGGCCGGggcggc                                               >  1:3877195/1‑101 (MQ=17)
                                                  tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGgcgc                                             >  2:3155880/1‑101 (MQ=18)
                                                  tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGgcgc                                             >  2:4546162/1‑101 (MQ=18)
                                                   gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGGGGCGGCGcc                                            >  1:2865893/1‑101 (MQ=17)
                                                     aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:4070976/101‑1 (MQ=17)
                                                     aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  1:2830502/1‑101 (MQ=18)
                                                     aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGACC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  2:4633550/1‑101 (MQ=18)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATGCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:229743/101‑1 (MQ=17)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         >  2:6531082/1‑101 (MQ=18)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:4103792/101‑1 (MQ=17)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:375861/101‑1 (MQ=17)
                                                      aCCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  2:1348192/101‑1 (MQ=17)
                                                       ccGGATGCTTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  2:3555338/101‑1 (MQ=17)
                                                        cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  1:4085201/101‑1 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:4260213/1‑101 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:3847130/1‑101 (MQ=17)
                                                            tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  1:1574503/101‑1 (MQ=17)
                                                             gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                  >  2:4196347/1‑101 (MQ=17)
                                                               cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  2:3644775/1‑101 (MQ=18)
                                                               cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  1:6252574/1‑101 (MQ=17)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGCGGGGGGC.AGCGCCC.CC.CTGCTCCGGAGGTCCGTGGCTGCGCCTTGACGCGCggggg                              >  2:5769270/1‑101 (MQ=9)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCTC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              <  1:1009584/101‑1 (MQ=18)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGCCGGGCggggg                              >  2:109252/1‑101 (MQ=17)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              <  1:1828257/101‑1 (MQ=18)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                             >  1:5355279/1‑100 (MQ=17)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:1717802/101‑1 (MQ=18)
                                                                  tCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  1:3608431/101‑1 (MQ=18)
                                                                     tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:6181575/101‑1 (MQ=18)
                                                                     tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcccg                          >  1:6141678/1‑101 (MQ=18)
                                                                      cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  2:2057294/1‑101 (MQ=18)
                                                                       ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        >  1:4032261/1‑101 (MQ=18)
                                                                       ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        <  2:316532/101‑1 (MQ=18)
                                                                        tgaccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCACC.CTGCCCCGGAGGAAAGTGAAGGCGCCTTGTCGGGCGGGGGGGCGGGc                       >  1:3451881/4‑101 (MQ=2)
                                                                        tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       >  2:1837980/1‑101 (MQ=18)
                                                                         gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCAC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  1:5227265/1‑101 (MQ=21)
                                                                         gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCa                      >  1:3185389/1‑101 (MQ=18)
                                                                          cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  1:4137702/101‑1 (MQ=18)
                                                                           ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:3557141/101‑1 (MQ=18)
                                                                           ccGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CA.AGGGGGGGGCAGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:1136679/101‑1 (MQ=21)
                                                                            cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:6480476/101‑1 (MQ=18)
                                                                             gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:1978730/101‑1 (MQ=18)
                                                                              aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCACC.CTGCCCCGGCGGCCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  2:5320501/1‑101 (MQ=18)
                                                                                 tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:4738294/1‑101 (MQ=21)
                                                                                   tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:5776040/101‑1 (MQ=21)
                                                                                     ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  1:3084703/101‑1 (MQ=21)
                                                                                      cGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg         <  1:6586212/101‑1 (MQ=25)
                                                                                       ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:4044295/1‑101 (MQ=25)
                                                                                       ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:1473541/1‑101 (MQ=25)
                                                                                       ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        <  1:6432116/101‑1 (MQ=25)
                                                                                        gggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCggagttccg       >  2:2270481/1‑93 (MQ=18)
                                                                                        gggggTGAA.GGGC..GAAG.CCAGAGGGGGGCAGCCC.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:2273409/101‑1 (MQ=25)
                                                                                          gggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:2053421/101‑1 (MQ=25)
                                                                                          gggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  2:2738464/101‑1 (MQ=25)
                                                                                           ggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  2:5429476/1‑101 (MQ=25)
                                                                                           ggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:3354257/101‑1 (MQ=25)
                                                                                           ggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:4227174/101‑1 (MQ=25)
                                                                                            gTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCCC.CC.CC.GTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAg   <  1:1641926/101‑1 (MQ=25)
                                                                                             tGAA.TGGC..GAAG.CCAGAGGGCGGC.AGCG.CC.CCTCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  <  1:4654149/101‑1 (MQ=37)
                                                                                                      |                                                                                                  
CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CC.CTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG