Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,080 +C 90.8% intergenic (+128/‑114) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0801.→C90.8% 54.7 0.0 ‑0.3218intergenic (+128/‑114)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (110/87):  ref base (11/9):  total (122/96)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.15e-01

Only 100 of 263 total aligned reads displayed.

GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170
                                                                                                        |                                                                                           
gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc                                                                                           >  1:2701939/1‑101 (MQ=18)
 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.c                                                                                         >  2:6585192/1‑101 (MQ=18)
    ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                                                      <  2:120243/101‑2 (MQ=17)
    ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccc                                                                                      <  2:1570929/101‑2 (MQ=17)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccct                                                                                     >  2:843344/1‑99 (MQ=18)
      ccccGGGATATTGGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                                    >  1:6308386/1‑98 (MQ=17)
        ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctgcc                                                                                  >  1:1756818/1‑96 (MQ=17)
         cGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.CC.CCCTGccc                                                                                  >  2:3513234/1‑101 (MQ=18)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 >  1:6612003/1‑101 (MQ=17)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 <  2:1733916/101‑1 (MQ=17)
           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                                                <  2:6340765/101‑1 (MQ=0)
           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                                                >  1:2857575/1‑101 (MQ=2)
           ggATATTTGCAGCAAGATGAAACAGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cc.cccctgccacg                                                                               >  1:3366503/1‑93 (MQ=14)
             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                                              <  1:6014660/101‑3 (MQ=2)
             atatTTGCAGCAAGATGAACCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                                                                              <  1:6198708/101‑1 (MQ=17)
              tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccg                                                                             >  2:4669774/1‑101 (MQ=2)
              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgccg                                                                             >  2:3164905/1‑101 (MQ=17)
               atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                                            >  1:3954242/1‑101 (MQ=2)
               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGCGGGCAGCCC.CC.CCCTGCCCcggcgg                                                                            >  2:6129407/1‑101 (MQ=18)
                tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGt                                                                           >  2:5128325/1‑101 (MQ=17)
                 ttGCAGCAAGATTAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                                                                          <  2:2494455/101‑1 (MQ=17)
                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                                                                          >  1:3639477/1‑101 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                                         <  1:6524599/101‑1 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                                         <  2:2441416/101‑1 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                                         <  2:4133783/101‑1 (MQ=17)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                                                                         <  1:6766555/101‑1 (MQ=17)
                      gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                                                     >  1:5794103/1‑101 (MQ=18)
                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                                    >  2:3891263/1‑101 (MQ=18)
                         aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                                                  >  1:3811002/1‑101 (MQ=17)
                          gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcggc                                                                 <  1:6883905/101‑1 (MQ=17)
                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCACGGCGGTCCGTAGCGgcgc                                                               >  2:1810558/1‑101 (MQ=17)
                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                                               >  2:4546162/1‑101 (MQ=18)
                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCAGTGGCGgcgc                                                               >  2:605370/1‑101 (MQ=17)
                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                              >  1:334723/1‑101 (MQ=18)
                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             >  2:2019594/1‑101 (MQ=18)
                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  2:4070976/101‑1 (MQ=17)
                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            >  1:4201836/1‑101 (MQ=17)
                                aCCGGATGCCTTTCATCTTGCCCGAAATATGCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           <  1:229743/101‑1 (MQ=17)
                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           <  1:4103792/101‑1 (MQ=17)
                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           >  2:6531082/1‑101 (MQ=18)
                                 ccGGATGCTTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          <  2:3555338/101‑1 (MQ=17)
                                 ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                          >  1:4464566/1‑101 (MQ=18)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  1:3521948/1‑101 (MQ=18)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGCGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  2:3456692/101‑1 (MQ=17)
                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                        >  2:4260213/1‑101 (MQ=17)
                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCGTGAcg                                                        >  1:3440069/1‑101 (MQ=17)
                                    gATGCCTTTCATCTTGCCCGAAATAGCCGGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       <  2:5558272/101‑1 (MQ=17)
                                     aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                                      <  2:2701843/101‑1 (MQ=17)
                                     aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                                      >  1:2970561/1‑101 (MQ=18)
                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   >  1:4061165/1‑101 (MQ=18)
                                         cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  >  2:3644775/1‑101 (MQ=18)
                                           ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGCGGGGGGC.AGCGCCC.CCCTGCTCCGGAGGTCCGTGGCTGCGCCTTGACGCGCggggg                                                >  2:5769270/1‑101 (MQ=9)
                                           ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCTC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                                                <  1:1009584/101‑1 (MQ=18)
                                           ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCCGGGCggggg                                                >  2:109252/1‑101 (MQ=17)
                                            tCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               <  1:3608431/101‑1 (MQ=18)
                                             cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:3206132/1‑101 (MQ=18)
                                             cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCGCCC.CCCTGCCCCGGCGGCCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:4780192/1‑101 (MQ=18)
                                               tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                            >  1:4692107/1‑101 (MQ=18)
                                               tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcccg                                            >  1:6141678/1‑101 (MQ=18)
                                                cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                                           >  2:2057294/1‑101 (MQ=18)
                                                  tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                         >  2:1837980/1‑101 (MQ=18)
                                                   gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                        >  1:5227265/1‑101 (MQ=21)
                                                    cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                                       >  2:2824829/1‑101 (MQ=18)
                                                     ccGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CA.AGGGGGGGGCAGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  1:1136679/101‑1 (MQ=21)
                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:5881703/101‑1 (MQ=18)
                                                       gAAATCGCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  2:2631363/101‑1 (MQ=18)
                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   <  2:3122171/101‑1 (MQ=18)
                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   <  2:2995275/101‑1 (MQ=18)
                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   <  2:1953456/101‑1 (MQ=18)
                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCACCCTGCCCCGGCGGCCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                   >  2:5320501/1‑101 (MQ=18)
                                                         aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                  >  1:609426/1‑101 (MQ=18)
                                                              cccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGGCCCGGCGGGCCGTGGCGGCGCGTTGGCGGGCGGGGGGGCGGGCGGTAAccagacg                             >  2:2270117/1‑96 (MQ=9)
                                                              cccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             >  1:1714053/1‑101 (MQ=21)
                                                               ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  1:3084703/101‑1 (MQ=21)
                                                               ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  1:1322901/101‑1 (MQ=21)
                                                                cGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGCAATTg                           >  2:5065239/1‑101 (MQ=21)
                                                                 ggggggTGAA.TGGC..GAAG.CCGGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          >  1:319282/1‑101 (MQ=25)
                                                                 ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          >  1:4044295/1‑101 (MQ=25)
                                                                  gggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:6177567/1‑101 (MQ=25)
                                                                  gggggTGAA.GGGC..GAAG.CCAGAGGGGGGCAGCCC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         <  1:2273409/101‑1 (MQ=25)
                                                                   ggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:6449688/1‑101 (MQ=25)
                                                                    gggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       <  1:2053421/101‑1 (MQ=25)
                                                                    gggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       <  1:1593894/101‑1 (MQ=25)
                                                                                 gcgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              >  2:3801790/11‑101 (MQ=38)
                                                                                  cgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             >  2:2369736/10‑101 (MQ=38)
                                                                                    aagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  1:4217239/8‑101 (MQ=38)
                                                                                    aagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  1:4549095/8‑101 (MQ=38)
                                                                                    aagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           <  2:2499618/94‑1 (MQ=38)
                                                                                     agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          <  1:1534891/95‑1 (MQ=38)
                                                                                     agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  1:916279/7‑101 (MQ=38)
                                                                                      gccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         >  1:247908/6‑101 (MQ=38)
                                                                                       ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:4831207/5‑101 (MQ=38)
                                                                                       ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:5377060/97‑1 (MQ=38)
                                                                                        cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  1:551457/4‑101 (MQ=38)
                                                                                        cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCGGCGATcccc       >  2:4226428/4‑99 (MQ=38)
                                                                                         agaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      <  1:7107821/99‑1 (MQ=39)
                                                                                           aGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:5668367/1‑101 (MQ=255)
                                                                                            ggggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   <  2:5868442/101‑1 (MQ=255)
                                                                                             gggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:4415851/1‑101 (MQ=255)
                                                                                             gggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:6430038/1‑101 (MQ=255)
                                                                                                        |                                                                                           
GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG