Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	121.2	38	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (4/34):  ref base (0/0):  total (4/34)

GTTCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                      |                                                                                                 gtgggggCGGAATAGGAACCGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                   <  2:329176/97‑1 (MQ=255) gtgcGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                   <  2:6131746/98‑1 (MQ=255) gtgcGGGCGAAATAGGAACCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                   <  2:2036860/98‑1 (MQ=255) gTTGGGGTGGAATAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                   <  2:4852256/101‑1 (MQ=255)  tgcGGGCGGAATAGGAACCGCGCCCCGGGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.g                                                                                                 <  1:1359781/99‑1 (MQ=255)   tggggCGAAATAGAAAACCCGCCCCGCGTTCCGCCGCCCGCAGCGCCCGGTTGCGGCATG.CCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                               <  1:3405422/99‑3 (MQ=37)    cGGGCGGAATAGGAACCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                               <  2:4605456/101‑3 (MQ=255)    cGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                               <  1:4223561/101‑3 (MQ=255)    cGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                               >  1:4382062/1‑99 (MQ=255)     gggCGGAATAGGAACCGCGGCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcg                                                                                              <  2:5850415/101‑4 (MQ=39)       gCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggg                                                                                            >  1:3245746/1‑96 (MQ=255)        gggAATAGGAACCGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggc                                                                                           <  2:3245630/100‑7 (MQ=38)        cGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggc                                                                                           >  1:5235702/1‑95 (MQ=255)           aaggggAACCGCGCCCCGCGTTGCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                         <  1:3161895/97‑1 (MQ=25)           aaTGGGAACCGCCCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                         <  1:6045925/101‑1 (MQ=25)           aaTAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                         <  1:3692838/101‑1 (MQ=37)           aaTAGGAACCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                         <  2:4453868/101‑1 (MQ=37)           aaTAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                         <  1:13313/101‑1 (MQ=25)            aTAGGACACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                        <  1:4871919/101‑1 (MQ=37)            aTAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                        <  2:4378050/101‑1 (MQ=37)             tAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                       <  1:4294205/101‑1 (MQ=37)             tAGGAACCGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                       <  2:5592964/101‑1 (MQ=37)             aaGGAAACGCCCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                       <  2:7014257/100‑1 (MQ=37)              agtaagcgcgCCCCTCGTCTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                      <  1:6597892/95‑1 (MQ=25)              aGGAAACGCGCCCCGCGTTGCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                      <  2:6869036/101‑1 (MQ=37)               ggAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                     <  1:2778250/101‑1 (MQ=25)               ggAAACGCGGCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                     <  1:3169872/101‑1 (MQ=37)               ggAAACGCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                     <  1:1265745/101‑1 (MQ=25)               ggAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                     <  2:3232245/101‑1 (MQ=37)                gAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcc                                                                                    >  2:5618293/1‑100 (MQ=255)                  accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                  <  2:5118470/99‑1 (MQ=25)                  accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                  <  2:6453239/99‑1 (MQ=25)                   aCGGGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                 <  2:6831639/101‑1 (MQ=25)                   aCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                 <  2:2901215/101‑1 (MQ=25)                    cgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                                <  1:5611129/101‑1 (MQ=25)                      cgccccCCGTCTCCCCGCCCCCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGTCGAGAAGcgcccgcc                                                                              <  1:5618583/98‑1 (MQ=21)                       gACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                            >  1:5354158/1‑101 (MQ=255)                       cccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                            <  1:4853413/99‑1 (MQ=25)                       cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                            <  1:4452096/99‑1 (MQ=25)                        ccccctttttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                           <  2:401784/94‑1 (MQ=25)                        ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                           <  1:6301453/100‑1 (MQ=255)                         cccccttccccccGCCCGCAGCGCCCGTTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                          <  1:650591/92‑2 (MQ=21)                         cccGCGTTCCCCCGCCCGCGGCCCCCGGTGGCGGCATGCCCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                          <  2:5353886/101‑2 (MQ=21)                           cccgtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                        <  1:5862280/94‑4 (MQ=21)                            ggttcgccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                       <  2:5536887/95‑5 (MQ=21)                            gggtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                       <  1:1130576/95‑5 (MQ=21)                            gcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                       <  1:5763948/95‑5 (MQ=21)                            cgcctgccccGCCCGCGGCGCCCGGTTGCGTCATGACCGTTTCAGCCCGCACAACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                       <  2:3361139/95‑5 (MQ=17)                             ggTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                      <  1:1011417/100‑6 (MQ=21)                             cgTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                      <  1:3454380/101‑6 (MQ=25)                               tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                    <  1:76691/98‑8 (MQ=21)                                                              gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGGGGTGGCCGAGGGCCCTTGGGCCCCTCggggt                                      >  2:3280255/1‑100 (MQ=17)                                                              gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGt                                     >  2:3177167/1‑101 (MQ=17)                                                                                  caGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCa                 >  2:4313916/1‑101 (MQ=17)                                                                                             gcccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGGCACAGGACCTCTGTGAAGCCTGGCCAAGGCg      >  1:613325/1‑101 (MQ=17)                                                                                              cccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGa     >  1:4768487/1‑101 (MQ=18)                                                                                                cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  2:2722409/1‑101 (MQ=18)                                                                                                cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  1:6657914/1‑101 (MQ=18)                                                                                                cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  1:4581203/1‑101 (MQ=18)                                                                                                 ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:2062315/1‑101 (MQ=18)                                                                                                 ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:757934/1‑101 (MQ=18)                                                                                                      |                                                                                                 GTTCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG