Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,869	+G	100%	intergenic (‑201/+92)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,869	1	.→G	100.0%	67.1	26	intergenic (‑201/+92)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (26/0):  ref base (0/0):  total (26/0)

CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962                                                                                                      |                                                                                               cgccccCCGTCTCCCCGCCCCCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGTCGAGAAGcgcccgcc                                                                                                 <  1:5618583/98‑1 (MQ=21)  gACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                               >  1:5354158/1‑101 (MQ=255)  cccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                               <  1:4853413/99‑1 (MQ=25)  cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                               <  1:4452096/99‑1 (MQ=25)   ccccctttttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                              <  2:401784/94‑1 (MQ=25)   ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                              <  1:6301453/100‑1 (MQ=255)    cccccttccccccGCCCGCAGCGCCCGTTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                             <  1:650591/92‑2 (MQ=21)    cccGCGTTCCCCCGCCCGCGGCCCCCGGTGGCGGCATGCCCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                             <  2:5353886/101‑2 (MQ=21)      cccgtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                           <  1:5862280/94‑4 (MQ=21)       ggttcgccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                                          <  2:5536887/95‑5 (MQ=21)       gggtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                                          <  1:1130576/95‑5 (MQ=21)       gcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                                          <  1:5763948/95‑5 (MQ=21)       cgcctgccccGCCCGCGGCGCCCGGTTGCGTCATGACCGTTTCAGCCCGCACAACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                                          <  2:3361139/95‑5 (MQ=17)        ggTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                                         <  1:1011417/100‑6 (MQ=21)        cgTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                                         <  1:3454380/101‑6 (MQ=25)          tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                                       <  1:76691/98‑8 (MQ=21)                                         gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGGGGTGGCCGAGGGCCCTTGGGCCCCTCggggt                                                         >  2:3280255/1‑100 (MQ=17)                                         gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGt                                                        >  2:3177167/1‑101 (MQ=17)                                                             caGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCa                                    >  2:4313916/1‑101 (MQ=17)                                                                        gcccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGGCACAGGACCTCTGTGAAGCCTGGCCAAGGCg                         >  1:613325/1‑101 (MQ=17)                                                                         cccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGa                        >  1:4768487/1‑101 (MQ=18)                                                                          ctgccccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt                       >  2:3850297/9‑101 (MQ=18)                                                                           cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                      >  2:2722409/1‑101 (MQ=18)                                                                           cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                      >  1:6657914/1‑101 (MQ=18)                                                                           cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                      >  1:4581203/1‑101 (MQ=18)                                                                            ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                     >  2:757934/1‑101 (MQ=18)                                                                            ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                     >  2:2062315/1‑101 (MQ=18)                                                                            gacccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGCCTCAGGACCCCTGTGACGCATGGCCAAGGGGATgg                     >  2:2899044/7‑101 (MQ=18)                                                                             gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                    >  1:5403698/6‑101 (MQ=18)                                                                                cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                 >  2:732402/3‑101 (MQ=21)                                                                                 ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                >  1:2804317/2‑101 (MQ=21)                                                                                  gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg               >  1:1332634/1‑101 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGGGATGGCCTTGGcc             >  2:3274935/4‑101 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             >  1:33007/4‑101 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             >  1:2446238/4‑101 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             >  2:1567750/4‑101 (MQ=21)                                                                                      gcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCaa           >  1:2549207/2‑101 (MQ=21)                                                                                        gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg         >  2:2612706/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  1:2570879/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  2:4604249/1‑101 (MQ=21)                                                                                          gaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc       >  2:2361209/1‑101 (MQ=21)                                                                                           aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa      >  2:3347017/1‑101 (MQ=21)                                                                                           aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa      >  2:4400065/1‑101 (MQ=21)                                                                                           aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa      >  1:1063759/1‑101 (MQ=21)                                                                                               gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc  >  2:1840958/1‑101 (MQ=21)                                                                                                      |                                                                                               CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 38 ≤  ATCG