Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,780,410 +T 1.5% coding (980/1257 nt) RCAP_rcc02591 ← surface presentation of antigens protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,780,4101.→T 1.5% 8.1 ‑3.5 ‑0.2273coding (980/1257 nt)RCAP_rcc02591surface presentation of antigens protein family
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (112/157):  total (116/157)
Fisher's exact test strand distribution p-value = 3.16e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.67e-01

Only 100 of 314 total aligned reads displayed.

CAGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780310‑2780510
                                                                                                                                                                                                           
 aGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCt                                                                                                       <  1:6128661/101‑1 (MQ=255)
 aGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCt                                                                                                       >  2:3950644/1‑101 (MQ=255)
  gACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCtt                                                                                                      <  1:1021928/101‑1 (MQ=255)
  gACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCtt                                                                                                      <  2:1523087/101‑1 (MQ=255)
   gccgccgCCACTGCCGGTCAGAGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTg                                                                                                     <  2:5827076/100‑1 (MQ=255)
    ccgccgCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTgg                                                                                                    <  1:6609375/101‑1 (MQ=255)
     cccccccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGc                                                                                                   <  1:1126230/96‑1 (MQ=255)
      gccgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCtttggc                                                                                                  >  2:1753244/1‑97 (MQ=255)
        cgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGgccc                                                                                                >  1:738762/1‑101 (MQ=255)
        cgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGcccc                                                                                                <  1:2866756/101‑1 (MQ=255)
        cgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGcccc                                                                                                <  2:3225486/101‑1 (MQ=255)
           cACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGcc                                                                                             <  2:5240503/101‑1 (MQ=255)
            aCTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCt                                                                                            <  2:5662049/101‑1 (MQ=255)
            aCTGCCGGGCAGCGGATCATAGGCCGGGGAATCGAGCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGccc                                                                                            >  1:6414257/1‑100 (MQ=255)
              tGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCg                                                                                          <  2:710854/101‑1 (MQ=255)
               gCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGa                                                                                         <  2:652035/101‑1 (MQ=255)
                   ggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGTCCCCccccccaggg                                                                                     >  1:3026073/1‑93 (MQ=255)
                   ggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCCCGGAggg                                                                                     >  1:978574/1‑101 (MQ=255)
                     cAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGTCCCGCCCCgcacgggg                                                                                   >  2:2924401/1‑94 (MQ=255)
                        cGGATCATAGGCAGGGGAATCGATCCGGCCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCGCGGCAAAGGGGCTTTGCCCCGCCTCGAAGggggggg                                                                                >  1:5066936/1‑99 (MQ=255)
                           aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCCCGGAGgggcgcgggc                                                                             >  2:4376676/1‑99 (MQ=255)
                            tCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCGCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCCCCCCCCAGGGGGGCGGCac                                                                            >  2:3451526/1‑101 (MQ=255)
                             cATGGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCacg                                                                           <  2:2756777/101‑1 (MQ=255)
                             cATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCacg                                                                           <  1:2133447/101‑1 (MQ=255)
                               tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCTCCCAGGGGGGCGGGacggc                                                                         >  2:203695/1‑99 (MQ=255)
                               tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCCCCCCGAAGGGCGGGGGGacgac                                                                         >  1:2432834/1‑101 (MQ=255)
                                aGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCacgacg                                                                        <  2:6783625/101‑1 (MQ=255)
                                  gCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCCCGCAGGGGGGGGGCCCGAggcg                                                                      >  2:3029569/1‑101 (MQ=255)
                                     ggggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCCCCAAGGGCGGGGGGGCGACgcgggg                                                                   >  2:5394656/1‑99 (MQ=255)
                                      gggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTTCCCCCCCCCGCATTGCGGCGGCCCCACACGgc.gc                                                                 >  2:862037/1‑101 (MQ=255)
                                      gggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGgc.gc                                                                 <  1:1730833/101‑1 (MQ=255)
                                       ggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCTCCGAGGGGGGCGGGGCGACGCGgc.gcc                                                                >  2:406832/1‑100 (MQ=255)
                                        gAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCCCCAAGGGGGGGGGGCCGACGCGGC.GCAc                                                               >  1:7034223/1‑101 (MQ=255)
                                          aTCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCCCGAAGGGGGGCGGCCCGACgcggc.ggcccc                                                             >  1:1809057/1‑96 (MQ=255)
                                            cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGTCCCCCCCCGGAGGGGGGGGGGGCGACGCGGC.GCACCctc                                                           >  1:1982015/1‑101 (MQ=255)
                                            cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGTCCCCCCCCCCCGGGGGGCCGCCCGGCCCGGG.GCACCctc                                                           >  1:3266275/1‑101 (MQ=255)
                                            cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttc                                                           <  2:6096559/101‑1 (MQ=255)
                                             gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCTCGGAGGGGGGCGGGACGACGCGGC.GCCCCttcg                                                          >  2:2667371/1‑101 (MQ=255)
                                             gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCTCGCAGGGGGGCGGGACGACGCGGC.GCGCCttcc                                                          >  2:4939601/1‑100 (MQ=255)
                                             gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCCCCCCCCAGGGGGGGGGCGCGACGCGGC.GCACCctcg                                                          >  1:393037/1‑101 (MQ=255)
                                             gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcg                                                          <  1:2687742/101‑1 (MQ=255)
                                               tCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCCCGAAGGGGGGCGGCGCGACGCGGC.ACACCctcggg                                                        >  2:1870678/1‑100 (MQ=255)
                                                ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCTCGAAGGGCGGCGGGACGACGCGGC.GGCCCctcgggt                                                       >  2:836806/1‑99 (MQ=255)
                                                ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCCCCCCGAAGGGCGGCGGCACGACGCGGC.GCGCCctcggtt                                                       >  1:4512487/1‑101 (MQ=255)
                                                ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggtt                                                       <  2:3675239/101‑1 (MQ=255)
                                                ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggtt                                                       <  1:5388313/101‑1 (MQ=255)
                                                 gatctgAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggttc                                                      <  2:3562279/96‑1 (MQ=255)
                                                 cGGTCGAGGGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggttc                                                      <  2:1198200/101‑1 (MQ=255)
                                                 cGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggttc                                                      <  2:1583868/101‑1 (MQ=255)
                                                  ggTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggttcg                                                     <  2:4569575/101‑1 (MQ=255)
                                                   gTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCttcggttcgg                                                    <  1:931837/101‑1 (MQ=255)
                                                     cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGGACGACGCGGC.GCACCCTCGGTTCgggg                                                  >  2:4593704/1‑100 (MQ=255)
                                                        gcggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGggcggc                                               <  2:2432740/101‑1 (MQ=255)
                                                         cggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCCCCTCGAAGGGCGGCGGCGCGACGCGGC.GCACCCTCGGGTCGGgcggcg                                              >  1:67439/1‑101 (MQ=255)
                                                          ggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGGACGACGCGGCGGCCCCTTCGG.TCGGGGGgcgc                                             >  2:2545044/1‑101 (MQ=255)
                                                            cgcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCa                                           <  1:825703/101‑1 (MQ=255)
                                                            cgcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCa                                           <  1:856950/101‑1 (MQ=255)
                                                               gcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCCCGAAGGGGGGCGGCACGACGCGGC.GGACCCTCGGGTCGGGGGGGGCCCTcc                                        >  1:3344830/1‑100 (MQ=255)
                                                               gcccgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCa                                        <  1:1046102/98‑1 (MQ=255)
                                                                cgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.g                                      <  2:2797237/101‑1 (MQ=255)
                                                                 gcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.Gc                                     <  2:6019779/101‑1 (MQ=255)
                                                                    tcccGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGTCCCCGCCTCGAAGGGCGTCGGTACGACGCGTC.GCTCCTTCGGTTCGGGCGGCGCCATCA.Gctct                                  <  2:823989/100‑1 (MQ=255)
                                                                    gCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCCTCCGTTCGGGGGGCGCCCTCC.Gctct                                  >  2:6271067/1‑101 (MQ=255)
                                                                     cccGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCtctt                                 <  1:3684539/101‑1 (MQ=255)
                                                                     cccGCGGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCtctt                                 <  1:5559011/101‑1 (MQ=255)
                                                                       cGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCGCCCCGAAGGGGGGCGGCACGACGCGGG.GCACCCTCGGGTCGGGGGGGGCCATCC.GCccctct                               >  1:1499331/1‑101 (MQ=255)
                                                                       cGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCtcttct                               <  2:420211/101‑1 (MQ=255)
                                                                        gCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTcttctt                              <  2:6418525/101‑1 (MQ=255)
                                                                        gCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTcttctt                              <  1:3410634/101‑1 (MQ=255)
                                                                        gCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCCCCCCGCAGGGCGGCGGCACGACGCCGC.GGGCCCTCGGTTCCGGCCGGGCCCTCACCCtcttct                               >  1:4436391/1‑101 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCCTCA.GCGCTTCTTcc                            >  2:3266626/1‑101 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCCTCGGTTCGGGCGGGGCCACCA.GCTcctctttc                            >  1:3547873/1‑99 (MQ=255)
                                                                           gggCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCt                           >  1:1253933/1‑101 (MQ=255)
                                                                           gggCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCt                           <  2:5533748/101‑1 (MQ=255)
                                                                           gggCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCt                           <  2:1104344/101‑1 (MQ=255)
                                                                            ggCGCCGATTTCCCGGCAAAGGGGCTTTGCCCCCCCCCGAAGGGGGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCCCTTCTTCCTc                          >  1:3901168/1‑101 (MQ=255)
                                                                            ggCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCCTCA.GCTcttctttccc                          >  2:4708167/1‑97 (MQ=255)
                                                                            ggCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTc                          <  2:2717662/101‑1 (MQ=255)
                                                                             gcgcCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGGGGCGGCACGACGCGGC.GCACCCTCGGTTTGGGCGGCGCCATCC.GCTCTTCTTTCTcc                         >  1:1700882/1‑100 (MQ=255)
                                                                              ggcCGATTTCCCGGCAAAGGAGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCgt                        <  1:5604955/100‑1 (MQ=255)
                                                                                 cGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGtt                     <  1:5878638/101‑1 (MQ=255)
                                                                                 cGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGtt                     <  2:4667076/101‑1 (MQ=255)
                                                                                  gATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCTCCCTCA.GCTCTTCTTCCTCGTGTTc                    >  1:4715022/1‑101 (MQ=255)
                                                                                  gATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTc                    <  2:3880721/101‑1 (MQ=255)
                                                                                  gATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTc                    <  2:2600785/101‑1 (MQ=255)
                                                                                  gATTTCCCGGCAAAGGGGCTTGGCCCCGCCCCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCCTGGTc                    >  1:6927633/1‑101 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCg                   <  1:2227953/101‑1 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCg                   <  1:533763/101‑1 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCCTCGGTTCGGGCGGGGCCCTCC.GCCCCTCTTCCCCGTGGTcc                   >  1:417933/1‑100 (MQ=255)
                                                                                    tttCCCGGCAAAGGGGCTTGGCCCCGCCCCGAAGGGGGGCGGCACGACGCGGC.GCACCTTCGGGTCGGGCGGCGCCCTCA.GCCCTTCTTccccccggtccc                  >  1:5216667/1‑91 (MQ=255)
                                                                                       cccGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCa               <  2:1705950/101‑1 (MQ=255)
                                                                                              aaGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAc        <  2:6203821/101‑1 (MQ=255)
                                                                                              aaGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAc        <  1:4285082/101‑1 (MQ=255)
                                                                                               aGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGTCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  2:341197/101‑1 (MQ=255)
                                                                                               aGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  1:6466238/101‑1 (MQ=255)
                                                                                               aGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  1:3768301/101‑1 (MQ=255)
                                                                                               aGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  1:4404079/101‑1 (MQ=255)
                                                                                               aGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCCTCGGGTCGGGCGGGGCCCTCA.GCTCTTCTTCCTCCTGTTCCATCAGccccccc       >  2:5940290/1‑96 (MQ=255)
                                                                                                 gggCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgc     >  2:1225812/1‑101 (MQ=255)
                                                                                                   gCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgcgc   >  1:2941495/1‑101 (MQ=255)
                                                                                                                                                                                                           
CAGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTGGCCCCGCCTCGAAGGGCGGCGGCACGACGCGGC.GCACCTTCGGTTCGGGCGGCGCCATCA.GCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780310‑2780510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG